Flow boiling in microchannels has been receiving a lot of attention in recent years because of its high heat flux removal capabilities at low flow rates and low pumping power. An important aspect of flow-boiling experiments is prediction or detection of the prevalent flow-regime. Currently, most researchers use high-speed camera for flow visualization for regime detection. However, in some cases due to limitations of the experimental setup and test-piece, such as multi-layer cooling of 3D IC stack, this may not be feasible. In this paper, the influence of flow-regime on frequency domain of local temperature data of the wetted surface is studied. Experiments have been performed synchronously with high speed flow visualization on a single microchannel with width and length of 2.54 mm and 25.4 mm respectively. The microchannel heights tested were 0.14,0.28 and 0.42mm. De-gassed, de-ionized water was used as the working fluid. Mass fluxes tested ranged from 200 to 1000 kg/(m2s). Depending on the prevalent flow regime, some of the highest of peak amplitudes in the frequency domain were quite distinct. Within the bounds of current experimental parameters, it is concluded that local transient temperature data can be a potential diagnostic tool for detection of flow-regimes. (A shorter version of this paper was presented at the 7th International Conference on Applied Energy (ICAE2015), March 28–31, 2015, Abu Dhabi, UAE (Original paper title: “Temperature transients for detection of flow-regimes in a mini/microchannel” and Paper No.: 430).)