High Glycemic Index Research Articles

Tribulus terrestris Fruit Extract: Bioactive Compounds, ADMET Analysis, and Molecular Docking with Penicillin-Binding Protein 2a Transpeptidase of Methicillin-Resistant Staphylococcus epidermidis

Tribulus terrestris is a rich source of bioactive molecules and thrives in Mediterranean and desert climate regions worldwide. In this study, Tribulus terrestris methanolic HPLC fractions were evaluated for bioactive compounds and PBP2a transpeptidase inhibitors against methicillin-resistant Staphylococcus epidermidis (MRSE). Among the collected HPLC fractions, F02 of the methanol extract demonstrated potential activity against MRSE01 (15 ± 0.13 mm), MRSE02 (13 ± 0.21 mm), and MRSE03 (16 ± 0.14 mm) isolates. GC-MS analysis of the F02 fraction identified seventeen compounds. Among seventeen compounds, eight have favorable pharmacokinetics and medicinal chemistry; however, on the basis of in silico high water solubility, high GI absorption, blood–brain barrier non-permeability, lack of toxicity, and potential drug-likeness, 1-ethylsulfanylmethyl-2,8,9-trioxa-5-aza-1-sila-bicyclo[3.3.3]undecane and phthalimide, N-(1-hydroxy-2-propyl), were processed for molecular docking. 1-ethylsulfanylmethyl-2,8,9-trioxa-5-aza-1-sila-bicyclo[3.3.3]undecane formed three hydrogen bonds with Ser-452, Thr-584, and Asn-454 residues of the PBP2a transpeptidase. Similarly, phthalimide, N-(1-hydroxy-2-propyl)-formed four hydrogen bonds with Ser-396, Asn-454, Lys-399, and Ser-452 residues of PBP2a transpeptidase. These two compounds are proposed as novel putative PBP2a transpeptidase inhibitors. Further characterization of compounds extracted from Tribulus terrestris may aid in identifying novel PBP2a inhibitory agents for managing MRSE infections.

Interaction between riluzole treatment and dietary glycemic index in the disease progression of amyotrophic lateral sclerosis.

We examined whether riluzole treatment modifies the associations between the dietary glycemic index (GI) and load (GL) and disease progression in amyotrophic lateral sclerosis (ALS). Sporadic ALS patients in the Multicenter Cohort Study of Oxidative Stress who completed a baseline food frequency questionnaire were included (n = 304). Interactions between baseline riluzole treatment and GI/GL on functional decline and tracheostomy-free survival were examined using linear regression and Cox proportional hazard models adjusted for covariates. Age, sex, disease duration, diagnostic certainty, body mass index, bulbar onset, revised ALS functional rating scale (ALSFRS-r) total score, and forced vital capacity, from baseline were included as covariates. Baseline higher GI and GL were associated with less decline of ALSFRS-r total score at 3-month follow-up in the riluzole treatment group (RTG) but not in the no-riluzole group (NRG). When quartile groups were used, GI second [β = -1.9, 95% CI (-4.1, -0.2), p = 0.07], third [β = -3.0, 95% CI (-5.1, -0.8), p < 0.01] and fourth [β = -2.2, 95% CI (-4.3, -0.01), p < 0.05] quartile groups were associated with less ALSFRS-r decline at 3-months compared to the first quartile group (GI < 47.2) among the RTG. Similarly, GL fourth quartile group (GL > 109.5) was associated with less ALSFRS-r decline at 3 months compared to the first quartile group [β = -2.6, 95% CI (-4.7, -0.5), p < 0.05] among the RTG. In NRG, no statistically significant differences in ALSFRS-r decline were found among GI/GL quartile groups. High dietary GI and GL are associated with a slower functional decline only among ALS patients taking riluzole.

Resistant starch's effect on blood glucose levels in individuals with prediabetes and diabetes mellitus: A systematic review

&lt;p&gt;&lt;strong&gt;&lt;em&gt;Background: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;Diabetes ranks among the ten leading causes of mortality. Patients with diabetes who do not effectively manage their condition run the risk of developing severe complications. An appropriate dietary regimen is considered a fundamental aspect of effectively managing diabetes. One potential treatment option for diabetics is resistant starch (RS), which is supposed to control blood glucose levels. &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Objectives: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;The objective of this study is to investigate the impact of resistant starch on food items characterized by a high glycemic index.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Methods: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;The methodology employed in this scholarly article is a systematic review. A comprehensive search was conducted across five prominent academic databases, namely EBSCO, PubMed, ProQuest, Scopus, and Google Scholar. The search strategy involved utilizing specific keywords, The presentation of the findings in this article adheres to the established guidelines and principles outlined in the Preferred Reporting Items for Systematic Review (PRISMA). &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Results:&lt;/em&gt;&lt;/strong&gt; &lt;em&gt;A total of eight statistically significant findings were observed, indicating that the consumption of starches effectively reduced blood sugar levels&lt;/em&gt;. The papers utilized genuine experimentation and quasi-experiment approaches within the past five years. &lt;em&gt;Numerous interventions involving resistant starch have demonstrated efficacy in the reduction of blood glucose levels. These interventions include the retrogradation of rice, the consumption of natural starch found in bananas and whole grains, as well as the addition of oil during the cooking process of rice. &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Conclusions: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;The inclusion of different forms of resistant starch in the dietary regimen of individuals with diabetes may be a viable option. However, it is important to acknowledge the significance of incorporating complementary food items in addition to the primary staple foods that are consumed. The utilization of resistant starch has been found to be an effective strategy for reducing blood glucose levels among individuals with diabetes.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;

Resistant starch's effect on blood glucose levels in individuals with prediabetes and diabetes mellitus: A systematic review

&lt;p&gt;&lt;strong&gt;&lt;em&gt;Background: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;Diabetes ranks among the ten leading causes of mortality. Patients with diabetes who do not effectively manage their condition run the risk of developing severe complications. An appropriate dietary regimen is considered a fundamental aspect of effectively managing diabetes. One potential treatment option for diabetics is resistant starch (RS), which is supposed to control blood glucose levels. &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Objectives: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;The objective of this study is to investigate the impact of resistant starch on food items characterized by a high glycemic index.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Methods: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;The methodology employed in this scholarly article is a systematic review. A comprehensive search was conducted across five prominent academic databases, namely EBSCO, PubMed, ProQuest, Scopus, and Google Scholar. The search strategy involved utilizing specific keywords, The presentation of the findings in this article adheres to the established guidelines and principles outlined in the Preferred Reporting Items for Systematic Review (PRISMA). &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Results:&lt;/em&gt;&lt;/strong&gt; &lt;em&gt;A total of eight statistically significant findings were observed, indicating that the consumption of starches effectively reduced blood sugar levels&lt;/em&gt;. The papers utilized genuine experimentation and quasi-experiment approaches within the past five years. &lt;em&gt;Numerous interventions involving resistant starch have demonstrated efficacy in the reduction of blood glucose levels. These interventions include the retrogradation of rice, the consumption of natural starch found in bananas and whole grains, as well as the addition of oil during the cooking process of rice. &lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Conclusions: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;The inclusion of different forms of resistant starch in the dietary regimen of individuals with diabetes may be a viable option. However, it is important to acknowledge the significance of incorporating complementary food items in addition to the primary staple foods that are consumed. The utilization of resistant starch has been found to be an effective strategy for reducing blood glucose levels among individuals with diabetes.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;

High Glycemic Index Foods Increase Oxidative Stress and Cause Chronic Musculoskeletal Pain

Chronic musculoskeletal pain is a widespread condition that impacts quality of life and poses significant health care challenges. Recent research shows that dietary factors, especially the consumption of high glycemic index (GI) foods, play an important role in exacerbating musculoskeletal pain through metabolic and inflammatory pathways. High GI foods cause a rapid spike in blood glucose, which causes increased production of reactive oxygen species (ROS) resulting in oxidative stress. Oxidative stress disrupts normal cellular function, increases the production of pro-inflammatory cytokines, and exacerbates chronic inflammation, a major driver of musculoskeletal pain. This review discusses the mechanisms by which high GI foods increase oxidative stress and contribute to the onset and persistence of chronic musculoskeletal pain and discusses oxidative damage increasing degradation of musculoskeletal tissue, delaying recovery, and worsening pain perception. Previous studies linking high GI diets to increased oxidative stress and the incidence of musculoskeletal pain provide evidence of the potential for dietary modification as a non-invasive therapeutic strategy. In conclusion, reducing foods with a high glycemic index and adopting a diet rich in antioxidants and low glycemic index to reduce oxidative stress and inflammation is very necessary. Understanding the impact of diet on oxidative stress offers a promising avenue for the prevention and treatment of chronic musculoskeletal pain. Further research is needed to strengthen this association and develop targeted dietary interventions for CMP patients.

Infrared treatment influence on the buckwheat groats structural and mechanical properties

One of the most popular grain crops is buckwheat. Buckwheat and its processed products have high digestibility, high nutritional value and low glycemic index, which is beneficial for people with diabetes mellitus types I and II. Buckwheat groats – a processed buckwheat product – are the second most popular on the market after rice. Based on the results of the studies, a technology for infrared processing of buckwheat has been developed. The presence of two areas of infrared radiation impact on buckwheat has been confirmed: the area of thermal destruction (steam formation) and the area of drying, when the water inside the product diffuses to the surface in the form of liquid and evaporates from the surface. The optimal parameters for intensive infrared processing of buckwheat have been established: radiant flux power of 32–34 kW/m², humidity of 14%, temperature of 135–140 °C; processing time of 35–40 s. It was found that the radiant flux density during IR treatment should not exceed 35 kW/m² to avoid burning of the cereal surface and deterioration of consumer qualities. It was found that the bulk density of buckwheat during IR treatment decreases by 1.6 times compared to untreated cereals. It was found that during infrared heat treatment of buckwheat, moisture evaporates, and its humidity decreases to 7.5–8.0%, which allows classifying it as a long-term storage product. It was found that during infrared treatment according to the developed method, the amount of extractive substances in buckwheat groats increases by 4 times.

Evaluation of glycemic index, glycemic load and biochemical traits of rice associated with anti-diabetic properties

Diabetes mellitus, a modern lifestyle disease and metabolic disorder, is closely associated with an increased risk of cardiovascular disease. Research on carbohydrates, particularly white rice with a high glycemic index, has been linked to an increased risk of type II diabetes, heart disease, and cancer. In this study, we aimed to understand the nutritional composition, estimated glycemic index, and glycemic load of twenty-eight rice accessions, particularly focusing on those with low starch digestibility associated with low GI levels. The proximate composition analysis revealed that tested rice accessions exhibited higher levels of genetic variation for amylose (18.45 - 25.97%), phenolic content (5.00-34.08%), protein (5.52-14.54%), and crude fibre (1.64-3.91%) content in brown rice. Huge variability for estimat?ed glycemic index, ranging from low to high GI was observed among all the varieties (49.37 - 78.58%). Traditional varieties viz., Thavalakannan and Kavuni depicted low estimated glycemic index (49.37 % and 54.55 %) and moderate glycemic load (14.60 and 15.80), respectively. The estimated glycemic index exhibited significant and negative association with amylose (r = -0.57**), phenolic (r = -0.67**), and crude fibre (r = -0.52**) content. In contrast, glycemic load showed a significant positive correlation with the amount of carbohydrate content. Principal component analysis revealed considerable variability among rice accessions' biochemical traits with the first two principal components accounting for 68.57% of the total variance. The hierarchical clustering based on Darwin software identified two major clusters. Cluster I comprise popular varieties and Cluster II contains traditional varieties with low to moderate glycemic index. Moreover, identifying rice varieties with lower glycemic index can facilitate the development and enhancement of breeding lines for the diabetic population.

A maternal sweet diet is associated with the gut dysbiosis in the first trimester of pregnancy

BackgroundThe composition of maternal gut phylum in each trimester of pregnancy has been associated with fetal development, separately. Diet is a main effective factor on the gut composition of phylum. However, associations between dietary glycemic index (GI), load (GL) and total antioxidant capacity (TAC) not studied with the gut population of phylum in mothers at the first trimester of pregnancy.Materials and methodsNinety healthy pregnant women aged 18–40 yrs, in the first trimester, were participated. Stool samples were gathered in a fasting state. Population of dominant phylum was determined after DNA extraction based on the 16SrRNA expression, as a housekeeping gene. Dietary intake was collected by a validated food frequency questionnaire and dietary indices were computed.ResultsThe Proteobacteria population was significantly higher in the gut of pregnant mothers than the other phylum (p < 0.001). Participants in the highest level of dietary GI had lower Bacteroidetes (p < 0.001) and Actinobacteria (p = 0.04) in their gut compared to the lowest level. Participants in the lowest level of dietary GL had higher Bacteroidetes (p < 0.001) and lower proteobacteria (p = 0.04) in their gut than the highest level. Dietary selenium showed a significant negative effect on the Firmicutes (p = 0.04) and Proteobacteria (p = 0.04), however positively affected the Actinobacteria (p = 0.01) population. Dietary zinc and manganese showed a negative effect on the Firmicutes population (p = 0.01 and p = 0.003). Zinc and vitamin E showed a negative effect on the Proteobacteria population (p = 0.04 and p = 0.03).ConclusionsA maternal diet with high GI and GL have been associated with the gut dysbiosis, however dietary intake of selenium, zinc, manganese and vitamin E act in favor of the intestinal eubiosis in the first trimester of pregnancy.

Comparison of glycaemic index of honey and sports energy products

Consumers' interest towards glycaemic index (GI) is expanding due to their aspiration towards informed buying and increased awareness regarding diet. This is especially the case for people engaged in various physical activities. Their diet, besides general principles of a healthy balanced diet, should also meet the needs of training. Consumption of carbohydrates with different GI before, during and after training affects athletic performance, with high GI food being preferred. The aim was to compare the effect of honey and two different sports energy products on glycaemia and their potential in terms of sports performance. GI of acacia honey and two sports energy products (gel and jelly) was determined according to the method ISO 26642: 2010. Ten healthy males aged 23.2 years (20 to 27 years old) who are minimum 4 hours per week engaged in at least one sport were enrolled. Glycaemia was the least affected by honey (GI = 89.6 ± 37.1), while jelly increased glycaemia the most (GI = 162.1 ± 60.9), and the difference was statistically significant (p = 0.008). Honey also showed good acceptability, and did not differ in subjective satiety score to gel or jelly. The results show that honey can be a good alternative to various sports energy products, and a more affordable one.

Combination effect of formulated high glycemic index diet with glibenclamide on fasting blood sugar, lipid profile and CYP2C9 in rats

Combination effect of formulated high glycemic index diet with glibenclamide on fasting blood sugar, lipid profile and CYP2C9 in rats

Maternal insulinemic and inflammatory dietary patterns and risk of child neurodevelopmental delay.

Our aim was to assess the risk of higher insulinemic, inflammatory, and hyperglycemia potential in the diet during pregnancy with child neurodevelopmental delay. We enrolled 7,438 pregnant women participating in a prospective cohort study. The food frequency questionnaire was used to evaluate the empirical dietary index for hyperinsulinemia (EDIH), empirical dietary inflammatory pattern (EDIP), glycemic index (GI), and glycemic load (GL) during mid-pregnancy. Child neurodevelopmental assessment was conducted at 6-36 months postpartum using the Denver Developmental Screening Test-II (DDST-II) scale, and the Gesell Developmental Diagnosis Scale (GDDS) was administered to assess children who did not meet the criteria for passing the DDST-II screening. We documented 540 incident child neurodevelopmental delay cases over 7,438 pregnant women (median follow-up: 2 years). Pregnant women exhibiting the high levels of hyperinsulinemic or proinflammatory components, or GI encountered an elevated risk of child neurodevelopmental delay; HRs (95% CI) comparing highest to lowest dietary index quintiles were EDIH 1.48 (1.07,2.04; P trend = 0.017), EDIP 1.39 (1.05,1.84; P trend = 0.019) and GI 1.36 (1.02,1.81; P trend = 0.038). In sex-stratified analyses, these results remained significant only in boys (P trend = 0.018 for EDIH, P trend = 0.028 for EDIP, P trend = 0.029 for GI). The performance of combined model of EDIH and EDIP for boys is comparable to that of the combined model of EDIH, EDIP, and GI to assess the risk of neurodevelopmental delay (AUC [area under curve] 0.801 vs. AUC 0.830). Both values are higher than the AUC values achieved by models using either set of indicators individually. Our findings suggest that maternal consumption of diets with high insulinemic, inflammatory, or glycemic index properties may be associated with neurodevelopmental delays in children, particularly in boys. Higher insulinemic and inflammatory potentials in maternal diet may forecast neurodevelopmental delay in boys.

Effect of chickpea on the physicochemical, nutritional, antioxidant, and organoleptic characterization of corn extrudates.

Ready-to-eat snacks are very popular. However, they have a high glycemic index and lack proteins & micronutrients. This study prepared protein-enriched corn extrudates by adding chickpea grit supplements at varying concentrations (0-100 g kg-1). The protein contents of 100 g kg-1 supplemented extrudates increased by 66.66% and dietary fiber contents increased by 48.02% in comparison with the control. Bulk density increased by 1.46 times. However, the expansion ratio, porosity, and water absorption index decreased significantly (P < 0.05). The health-promoting characteristics of the extrudates increased in comparison with the control sample, i.e., total phenolic content increasing by 17.84%, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) contents by 11.38%, and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) content by 9.59%. Likewise, the potassium contents increased by 24.63% with the inclusion of 10% chickpea in corn extrudates. Sensory evaluation revealed that corn extrudates with up to 60 g kg-1 added chickpea achieved the highest acceptability among panelists. The addition of chickpea produced corn extrudates with higher protein and mineral content, which could mitigate malnutrition. © 2024 Society of Chemical Industry.

Pre-exercise ingestion of a low glycaemic index rice-based mixed meal increases fat oxidation and endurance running performance in a hot-humid environment.

Relative to exercise in a thermoneutral environment, there is only limited evidence demonstrating that a low glycaemic index (LGI) pre-exercise meal can enhance endurance exercise performance in a hot-humid (HH) condition. Also, previous studies predominantly utilised Western-based and single food items, with minimal focus on Asian-based mixed meals. This study aimed to investigate the impact of pre-exercise LGI and high glycaemic index (HGI) rice-based mixed meals on endurance performance among acclimatised trained athletes in HH condition (32 °C, 65% relative humidity). Twelve native-born endurance-trained male runners (age 22.0±5.8 years; peak oxygen consumption (V̇O2peak) 64.2±5.5mL kg-1 min-1) completed two trials consisting of 45 min steady-state (SS) run at 70% V̇O2peak followed by 10km performance run (TT10km). Three hours before exercise, participants consumed an isocaloric rice-based mixed meals containing either LGI (GI value=47) or HGI (GI value=80), providing 1.3g of carbohydrate (CHO) per kg of body mass. Participants ran faster during TT10km after consuming the LGI meal compared to the HGI meal (LGI: 55.18±1.22vs. HGI: 57.03±2.25min, p=0.010). End rectal temperature did not significantly differ between trials (LGI: 39.16±0.74vs. HGI: 38.95±0.46 °C, p=0.352). Fat oxidation was higher during the SS run in the LGI compared to the HGI trial (LGI: 0.19±0.05vs. HGI: 0.13±0.19g min-1, p=0.001). This study demonstrated that, relative to HGI, consuming a pre-exercise LGI rice-based mixed meal enhanced endurance performance in HH environment among acclimatised trained male athletes.

The combination of metformin and high glucose increased longevity of Caenorhabditis elegans a DAF-16/FOXO-independent manner: cancer/diabetic model via C. elegans.

Sedentary lifestyles and diets with high glycemic indexes are considered to be contributing factors to the development of obesity, type 2 diabetes in humans. Metformin, a biguanide medication commonly used to treat type 2 diabetes, has been observed to be associated with longevity; however, the molecular mechanisms underlying this observation are still unknown. The effects of metformin and high glucose, which have important roles in aging-related disease such as diabetes and cancer, were studied in lin-35 worms because they are associated with cancer-associated pRb function in mammals and have a tumour suppressor property. According to our results, the negative effect of high glucose on egg production of lin-35 worms was greater than that of N2 worms. High glucose shortened lifespan and increased body length and width in individuals of both strains. Metformin treatment alone extended the lifespan of N2 and lin-35 worms by reducing fertilization efficiency. However, when metformin was administered in the presence of high glucose, the lifespan of lin-35 worms was clearly longer compared to N2 worms. Additionally, we conclude that glucose and metformin in lin35 worms can extend life expectancy through a DAF-16/FOXO-independent mechanism. Furthermore, the results of this study will provide a new perspective on extending mammalian lifespan through the model organism C. elegans.

Relevance of high glycaemic index breakfast for heart rate variability among collegiate students with early and late chronotypes

ABSTRACT Plasma glucose spikes affect cardiac autonomic modulation resulting in a decrease of heart rate variability (HRV). We hypothesize that a later chronotype or a higher morning plasma melatonin level is associated with larger decreases of HRV following an early high glycaemic index (GI) breakfast. In persons with an early (n = 21) or a late (n = 15) chronotype who consumed a high GI breakfast at 7 a.m. glucose data were continuously monitored. Time domain HRV parameters were calculated from blood volume pulses derived by wireless wrist worn multisensor. HRV changes (values after minus values before breakfast) were associated with chronotype by multivariable linear regression adjusted for age, sex and baseline levels. Morning plasma melatonin levels were determined from samples drawn on the run-in day. Time domain parameters indicate a higher HRV before high GI breakfast in both chronotypes. A later chronotype tended to be associated with smaller decreases of mean interbeat intervals (p = 0.08) only; no associations were seen with morning melatonin levels. This exploratory analysis in a small sample provides a first indication that in young healthy adults later chronotype might be associated with reduced ANS activation following a high GI breakfast. Future studies should elucidate whether this indicates parasympathetic or sympathetic inhibition.

Dietary quality, perceived health, and psychological status as key risk factors for newly developed metabolic dysfunction–associated steatotic liver disease in a longitudinal study

ObjectivesThis study investigated biomarkers in individuals with newly developed metabolic dysfunction–associated steatotic liver disease (ND-MASLD) and examined the interplay between genetic predisposition and environmental factors using a machine learning approach in a large longitudinal study. MethodsParticipants were classified into four groups based on metabolic dysfunction–associated steatotic liver disease (MASLD) status between the first and second measurements with an approximate 5-y gap. A model was developed to identify early-stage biomarkers of ND-MASLD (n = 1603). Nutrient intake, dietary patterns, genetic variants, and psychosocial factors were compared among the no MASLD (n = 60 081), recovered MASLD (n = 3181), persistent MASLD (n = 670), and ND-MASLD (n = 1603) groups. Their association with ND-MASLD was also predicted using a machine learning approach. ResultsThe model incorporating ND-MASLD status, age, sex, dietary inflammatory index, and metabolic syndrome (MetS), especially low high-density lipoprotein cholesterol and hypertriglyceridemia, at the second measurement demonstrated an optimal fit. High carbohydrate intake with a high glycemic index was associated with elevated ND-MADSLD risk. Fatty liver index was lower in persistent MASLD followed by ND-MASLD, recovered MASLD, and no MASLD. Participants in the ND-MASLD group had lower vitamin D and total isoflavonoid intake and a lower modified healthy eating index, indicating unhealthy diets. The XGBoost and deep neural network models identified age, sex, MetS components, dietary antioxidants, self-rated health, psychological well-being indexes, and serum liver enzyme levels at the second measurement as significant predictors of ND-MASLD. However, polygenic risk scores were not included. ConclusionsEarly-stage biomarkers of ND-MASLD were closely linked to MetS incidence. Dietary quality, perceived health status, and psychological stress emerged as potential targets for MASLD prevention strategies, with lifestyle modifications potentially overriding genetic predispositions. The results indicate that preventive strategies about lifestyle modification should be developed for MASLD.

A low-carbohydrate, high-fat diet leads to unfavorable changes in blood lipid profiles compared to carbohydrate-rich diets with different glycemic indices in recreationally active men.

In addition to recent discussions of low-carbohydrate, high-fat diets (LCHF) from a performance perspective, there is a paucity of knowledge regarding influence of the combined effect of an exercise and nutritional intervention, which varies in carbohydrate (CHO) intake and glycemic indices, on blood lipid levels in recreationally active men. A total of 65 male runners (VO2 peak = 55 ± 8 mL·min-1·kg-1) completed a 10-week ad libitum nutritional regimen (LOW-GI: ≥ 65% low GI CHO per day, n = 24; HIGH-GI: ≥ 65% high GI CHO per day, n = 20; LCHF: ≤ 50 g CHO daily, n = 21) with a concurrent prescribed endurance training intervention. Fasting total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were determined before and after the intervention. Additionally, 24-h dietary recalls were completed twice weekly. Following the intervention, TC was significantly higher in LCHF (196 ± 37 mg·dL-1) compared to both LOW-GI (171 ± 41 mg·dL-1) and HIGH-GI (152 ± 28 mg·dL-1, p < 0.001). Additionally, LDL-C levels increased in LCHF (+17 ± 21 mg·dL-1, p = 0.001), while they decreased in both CHO groups (p < 0.05, respectively). Only the HIGH-GI group demonstrated a significant reduction in HDL-C (-3 ± 9 mg·dL-1, p = 0.006), while a decrease in TG was only significant in LOW-GI (-18 ± 36 mg·dL-1, p = 0.008). Although mean blood lipid levels remained within the normal range, the data indicate that a low-carbohydrate, high-fat (LCHF) diet leads to unfavorable changes in individual blood lipid profiles compared to carbohydrate-rich diets. Therefore, it is recommended that the impact of a low-carbohydrate diet on blood lipids be considered when counseling active and healthy individuals.

Orange-flesh sweet potato powder as a promising partial substitution rice flour to produce high quality and low glycemic index vermicelli

This study aims to improve the quality of vermicelli products by replacing a portion of rice flour with orange-fleshed sweet potato powder at four ratios ranging from 10 % (F1) to 25 % (F4) (5 % interval), while the control sample used 100 % rice flour (F0). The products were analyzed for quality and sensory characteristics. The analysis of five vermicelli production formulas revealed that the product made from formula F3, which contains OFSP powder and rice flour at a ratio of 20:80 %, had a relatively high amount of β-carotene (9.29 μg/g), a bright color (L∗ = 67.15, b∗ = 29.45), and a suitable hardness (46.79 g-force). The F3 sample had better cooking quality than the F0 sample, which was proved by the percentages of weight increase, volume increase, and dry matter loss of 59.88 %, 56.89 %, and 7.08 %, respectively. The estimated PRAL score of the product prepared from formula F3 was −1.45 (PRAL<0), indicating that the vermicelli product is alkaline, which can support and improve human health. A quantity of 100 g of vermicelli contains 25.15 g of carbohydrates, 3.17 g of protein, 1.48 g of lipids, 4.26 g of resistant starch, 2.43 g of fiber, and 1.66 g of ash. The reduction in estimated glycemic index (eGI) was also found, reaching a value of 51.24 for the F3 sample, whereas the eGI of F0 was 58.24 (medium-high range). Furthermore, microstructural image analysis (from a scanning electron microscope) of sample F3 indicated that OFSP powder addition could result in a better gel network structure than occurs in conventional vermicelli. The findings from this study will be a steppingstone to processing various popular food products with enhanced nutrients. This activity can also be developed on a larger production scale, improving the quality of life for communities, especially in areas with chronic vitamin A deficiency.

7970 C3 and C4 Levels Decrease Post-prandial in Adolescents with type 1 Diabetes

Abstract Disclosure: C.P. Damilano: None. C. Yu: None. D. Zhou: None. R.P. Hoffman: None. Introduction: Cardiovascular disease will be the primary cause of future death for adolescents with type 1 diabetes (T1D) and its origins are likely to occur in this age group. Adolescents with T1D have increased systemic inflammation which is likely to play a significant role in the pathogenesis of cardiovascular disease. The complement system, in particular, components C3 and C4 are part of the innate immune system that regulates inflammation. Increased C3 and C4 levels are associated with increased cardiometabolic risk. Objective: Most studies of inflammation are done while fasting but TNFα and IL6 increase postprandially in adolescent T1D following both high and low glycemic index (GI) meals. The goal of this study was to determine whether there are similar post-prandial changes in C3 and C4 levels. Methodology: Twelve, non-obese children and adolescents, 10-18 years old, with T1D were recruited from the Diabetes Clinic of Nationwide Children's Hospital. A randomized cross-over design for their two subsequent visits was used. After an overnight fast baseline blood samples were taken to measure plasma glucose, C3, total C4 protein, and total C4. Rapid acting insulin was given to cover meal carbohydrate without glucose correction, and blood samples were repeated (30, 60, 90, and 120 minutes) to measure glucose and C3 and C4 levels. Visits 1 and 2 were identical except for GI of the given meal. Visits were rescheduled if the fasting glucose was greater than 200 mg/dl. Repeated measures ANOVA and Spearman’s correlation were used. Results: Glucose response was significantly greater following high GI meal (Δglucose: high 52±12 vs low 6±5 mg/dl, mean±SE, p&amp;lt;0.001). For C3 levels, there was a significant post-prandial decrease (time effect p=0.049), but no meal effect or meal x time interaction. No specific time differences were found. C4 levels were also suppressed postprandially (p&amp;lt;0.001) and there was again no meal effect or meal by time interaction. Post hoc analysis found that C4 levels were lower at 60 min than at 0 min (p=0.007), 90 min (p=0.002) or 120 min (p&amp;lt;0.001). Conclusions: C3 and C4 levels decrease post-prandially in adolescents with T1D in contrast to increases in TNFα and IL6. Future research is clearly needed to clarify the postprandial interactions between the complement and inflammatory systems and potential pathogenic role in cardiovascular disease. Presentation: 6/3/2024

The association of dietary insulinemic indices with PI3K, PTEN, and Akt gene expressions in visceral and subcutaneous adipose tissues among individuals undergoing abdominal surgery.

The current study investigates the association between dietary insulinemic indices and Akt, PTEN, and PI3K gene expressions in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) among individuals undergoing abdominal surgery. This cross-sectional study was conducted on 176 individuals, aged 18-84 years, who had undergone abdominal surgery. The participants were classified based on body mass index (BMI) as normal (BMI < 25 kg/m2), overweight (BMI = 25-29.9 kg/m2), and obese (BMI ≥ 30 kg/m2). The food frequency questionnaire was used to determine dietary glycemic and insulinemic indices. Real-time polymerase chain reaction was conducted for the expression of PI3K, PTEN, and Akt genes. In the final adjusted model, in normal-weight patients, there was an inverse relationship between the lifestyle with a higher insulinemic potential and the PI3K gene expression in VAT. In addition, there was an inverse association between dietary insulin load and the Akt gene expression in VAT. However, a higher glycemic index was positively associated with the PTEN gene expression in VAT. In overweight patients, a high insulinemic potential of the diet was associated with higher PTEN gene expression in VAT. In obese individuals, there were positive associations between lifestyle index for insulin resistance and hyperinsulinemia and the PI3K gene expression in VAT. Moreover, the higher insulinemic potential of diet and lifestyle was positively related to a higher expression of the PTEN and Akt genes in VAT. Our findings revealed that high insulinemic lifestyles and dietary patterns may be related to the expression of PI3K, PTEN, and Akt in adipose tissues.