High Fire Frequency Research Articles

Fire structures pine serotiny at different scales

Serotiny (delayed seed release with the consequent accumulation of a canopy seedbank) confers fitness benefits in environments with crown-fire regimes. Thus, we predicted that serotiny level should be higher in populations recurrently subjected to crown-fires than in populations where crown-fires are rare. In addition, under a high frequency of fires, space and resources are recurrently available, permitting recruitment around each mother to follow the seed rain shadow. Thus, we also predicted spatial aggregation of serotiny within populations. We compared serotiny, considering both the proportion and the age of serotinous cones, in populations living in contrasting fire regimes for two iconic Mediterranean pine species (Pinus halepensis, P. pinaster). We framed our results by quantitatively comparing the strength of the fire-serotiny relationship with previous studies worldwide. For the two species, populations living under high crown-fire recurrence regimes had a higher serotiny level than those populations where the recurrence of crown-fires was low. For P. halepensis (the species with higher serotiny), populations in high fire recurrence regimes had higher fine-scale spatial aggregation of serotiny than those inhabiting low fire recurrence systems. The strength of the observed fire-serotiny relationship in P. halepensis is among the highest in published literature. Fire regime shapes serotiny level among populations, and in populations with high serotiny, recurrent fires maintain a significant spatial structure for this trait. Consequently, fire has long-term evolutionary implications at different scales, emphasizing its prominent role in shaping the ecology of pines.

Abrupt and gradual transitions between low and hyperexcited firing frequencies in neuronal models with fast synaptic excitation: A comparative study

Hyperexcitability of neuronal networks is one of the hallmarks of epileptic brain seizure generation, and results from a net imbalance between excitation and inhibition that promotes excessive abnormal firing frequencies. The transition between low and high firing frequencies as the levels of recurrent AMPA excitation change can occur either gradually or abruptly. We used modeling, numerical simulations, and dynamical systems tools to investigate the biophysical and dynamic mechanisms that underlie these two identified modes of transition in recurrently connected neurons via AMPA excitation. We compare our results and demonstrate that these two modes of transition are qualitatively different and can be linked to different intrinsic properties of the participating neurons.

Electrophysiological Heterogeneity of Fast-Spiking Interneurons: Chandelier versus Basket Cells

In the prefrontal cortex, parvalbumin-positive inhibitory neurons play a prominent role in the neural circuitry that subserves working memory, and alterations in these neurons contribute to the pathophysiology of schizophrenia. Two morphologically distinct classes of parvalbumin neurons that target the perisomatic region of pyramidal neurons, chandelier cells (ChCs) and basket cells (BCs), are generally thought to have the same “fast-spiking” phenotype, which is characterized by a short action potential and high frequency firing without adaptation. However, findings from studies in different species suggest that certain electrophysiological membrane properties might differ between these two cell classes. In this study, we assessed the physiological heterogeneity of fast-spiking interneurons as a function of two factors: species (macaque monkey vs. rat) and morphology (chandelier vs. basket). We showed previously that electrophysiological membrane properties of BCs differ between these two species. Here, for the first time, we report differences in ChCs membrane properties between monkey and rat. We also found that a number of membrane properties differentiate ChCs from BCs. Some of these differences were species-independent (e.g., fast and medium afterhyperpolarization, firing frequency, and depolarizing sag), whereas the differences in the first spike latency between ChCs and BCs were species-specific. Our findings indicate that different combinations of electrophysiological membrane properties distinguish ChCs from BCs in rodents and primates. Such electrophysiological differences between ChCs and BCs likely contribute to their distinctive roles in cortical circuitry in each species.

Notes on the taxonomic and ecological significance of bark structure in the genus Virgilia (Fabaceae, Podalyrieae)

A comparative study of bark anatomy of all three taxa of the genus Virgilia has revealed interesting differences relating to the taxonomic relationships of the species and subspecies and also to the marked ecological differences (as reflected in the bark anatomy) between Virgilia oroboides subsp. oroboides (exposed to high fire frequencies in the western parts of the Cape Province of South Africa, from Cape Town to Swellendam) and V. oroboides subsp. ferruginea and Virgilia divaricata (growing in areas of relatively low fire frequency along the southern Cape coast from George to Port Elizabeth). The conspicuous difference in the appearance of the bark (thick and corky in V. oroboides subsp. oroboides; thin and smooth in V. oroboides subsp. ferruginea and V. divaricata) results from a distinct difference in the sclerification of periderm cells. Detailed descriptions of the bark anatomy of all three taxa are presented, showing a pattern of variation that supports the delimitation of species and subspecies. The discovery of crystals in the trichome cells of V. oroboides subsp. ferruginea appears to be a first record of the presence of crystals inside trichomes in the Fabaceae and is a useful diagnostic character for the subspecies. A remarkable feature of the bark of Virgilia is the common occurrence of acicular crystals arranged in radial (sheath-like) aggregates.

Chronic Alterations in Monoaminergic Cells in the Locus Coeruleus in Orexin Neuron-Ablated Narcoleptic Mice

Narcolepsy patients often suffer from insomnia in addition to excessive daytime sleepiness. Narcoleptic animals also show behavioral instability characterized by frequent transitions between all vigilance states, exhibiting very short bouts of NREM sleep as well as wakefulness. The instability of wakefulness states in narcolepsy is thought to be due to deficiency of orexins, neuropeptides produced in the lateral hypothalamic neurons, which play a highly important role in maintaining wakefulness. However, the mechanism responsible for sleep instability in this disorder remains to be elucidated. Because firing of orexin neurons ceases during sleep in healthy animals, deficiency of orexins does not explain the abnormality of sleep. We hypothesized that chronic compensatory changes in the neurophysiologica activity of the locus coeruleus (LC) and dorsal raphe (DR) nucleus in response to the progressive loss of endogenous orexin tone underlie the pathological regulation of sleep/wake states. To evaluate this hypothesis, we examined firing patterns of serotonergic (5-HT) neurons and noradrenergic (NA) neurons in the brain stem, two important neuronal populations in the regulation of sleep/wakefulness states. We recorded single-unit activities of 5-HT neurons and NA neurons in the DR nucleus and LC of orexin neuron-ablated narcoleptic mice. We found that while the firing pattern of 5-HT neurons in narcoleptic mice was similar to that in wildtype mice, that of NA neurons was significantly different from that in wildtype mice. In narcoleptic mice, NA neurons showed a higher firing frequency during both wakefulness and NREM sleep as compared with wildtype mice. In vitro patch-clamp study of NA neurons of narcoleptic mice suggested a functional decrease of GABAergic input to these neurons. These alterations might play roles in the sleep abnormality in narcolepsy.

Biomass and Its Allocation in Relation to Temperature, Precipitation, and Soil Nutrients in Inner Mongolia Grasslands, China

AimUnderstanding and predicting ecosystem functioning such as biomass accumulation requires an accurate assessment of large-scale patterns of biomass distribution and partitioning in relation to climatic and soil environments.MethodsWe sampled above- and belowground biomass from 26 sites spanning 1500 km in Inner Mongolian grasslands, compared the difference in aboveground, belowground biomass and below-aboveground biomass ratio (AGB, BGB, and B/A, respectively) among meadow steppe, typical steppe, and desert steppe types. The relationships between AGB, BGB, B/A and climatic and soil environments were then examined.ResultsWe found that AGB and BGB differed significantly among three types of grasslands while B/A did not differ. Structural equation model analyses indicated that mean annual precipitation was the strongest positive driver for AGB and BGB. AGB was also positively associated with soil organic carbon, whereas B/A was positively associated with total soil nitrogen.ConclusionsThese results indicated that precipitation positively influence plant production in Inner Mongolian grasslands. Contrary to the prediction from the optimal partitioning hypothesis, biomass allocation to belowground increased with soil total nitrogen, suggesting that more productive sites may increase belowground allocation as an adaptive strategy to potentially high fire frequencies.

Recent burning of boreal forests exceeds fire regime limits of the past 10,000 years

Wildfire activity in boreal forests is anticipated to increase dramatically, with far-reaching ecological and socioeconomic consequences. Paleorecords are indispensible for elucidating boreal fire regime dynamics under changing climate, because fire return intervals and successional cycles in these ecosystems occur over decadal to centennial timescales. We present charcoal records from 14 lakes in the Yukon Flats of interior Alaska, one of the most flammable ecoregions of the boreal forest biome, to infer causes and consequences of fire regime change over the past 10,000 y. Strong correspondence between charcoal-inferred and observational fire records shows the fidelity of sedimentary charcoal records as archives of past fire regimes. Fire frequency and area burned increased ∼6,000-3,000 y ago, probably as a result of elevated landscape flammability associated with increased Picea mariana in the regional vegetation. During the Medieval Climate Anomaly (MCA; ∼1,000-500 cal B.P.), the period most similar to recent decades, warm and dry climatic conditions resulted in peak biomass burning, but severe fires favored less-flammable deciduous vegetation, such that fire frequency remained relatively stationary. These results suggest that boreal forests can sustain high-severity fire regimes for centuries under warm and dry conditions, with vegetation feedbacks modulating climate-fire linkages. The apparent limit to MCA burning has been surpassed by the regional fire regime of recent decades, which is characterized by exceptionally high fire frequency and biomass burning. This extreme combination suggests a transition to a unique regime of unprecedented fire activity. However, vegetation dynamics similar to feedbacks that occurred during the MCA may stabilize the fire regime, despite additional warming.

Metabolic Maturation of Auditory Neurones in the Superior Olivary Complex

Neuronal activity is energetically costly, but despite its importance, energy production and consumption have been studied in only a few neurone types. Neuroenergetics is of special importance in auditory brainstem nuclei, where neurones exhibit various biophysical adaptations for extraordinary temporal precision and show particularly high firing rates. We have studied the development of energy metabolism in three principal nuclei of the superior olivary complex (SOC) involved in precise binaural processing in the Mongolian gerbil (Meriones unguiculatus). We used immunohistochemistry to quantify metabolic markers for energy consumption (Na+/K+-ATPase) and production (mitochondria, cytochrome c oxidase activity and glucose transporter 3 (GLUT3)). In addition, we calculated neuronal ATP consumption for different postnatal ages (P0–90) based upon published electrophysiological and morphological data. Our calculations relate neuronal processes to the regeneration of Na+ gradients perturbed by neuronal firing, and thus to ATP consumption by Na+/K+-ATPase. The developmental changes of calculated energy consumption closely resemble those of metabolic markers. Both increase before and after hearing onset occurring at P12–13 and reach a plateau thereafter. The increase in Na+/K+-ATPase and mitochondria precedes the rise in GLUT3 levels and is already substantial before hearing onset, whilst GLUT3 levels are scarcely detectable at this age. Based on these findings we assume that auditory inputs crucially contribute to metabolic maturation. In one nucleus, the medial nucleus of the trapezoid body (MNTB), the initial rise in marker levels and calculated ATP consumption occurs distinctly earlier than in the other nuclei investigated, and is almost completed by hearing onset. Our study shows that the mathematical model used is applicable to brainstem neurones. Energy consumption varies markedly between SOC nuclei with their different neuronal properties. Especially for the medial superior olive (MSO), we propose that temporally precise input integration is energetically more costly than the high firing frequencies typical for all SOC nuclei.

Landscape‐scale eco‐evolutionary dynamics: Selection by seed predators and fire determine a major reproductive strategy

Recent work in model systems has demonstrated significant effects of rapid evolutionary change on ecological processes (eco-evolutionary dynamics). Fewer studies have addressed whether eco-evolutionary dynamics structure natural ecosystems. We investigated variation in the frequency of serotiny in lodgepole pine (Pinus contorta), a widespread species in which postfire seedling density and ecosystem structure are largely determined by serotiny. Serotiny, the retention of mature seeds in cones in a canopy seed bank, is thought to be an adaptation for stand-replacing fire, but less attention has been paid to the potential selective effects of seed predation on serotiny. We hypothesized that spatial variation in percentage serotiny in lodgepole pine forests results from an eco-evolutionary dynamic where the local level of serotiny depends on the relative strengths of conflicting directional selection from fire (favoring serotiny) and seed predation (favoring cones that open at maturity). We measured percentage serotiny, the abundance of American red squirrels (Tamiasciurus hudsonicus; the primary pre-dispersal seed predator of lodgepole pine), and several measures of forest structure in Yellowstone National Park, USA. Fire frequency strongly predicted the frequency of serotiny, a pattern that is well-supported in the literature. At sites with high fire frequency (return intervals of -135-185 years) where fire favors increased serotiny, squirrel abundance was negatively associated with serotiny, suggesting that selection from predation can overwhelm selection from fire when squirrels are abundant. At sites with low fire frequency (return intervals of -280-310 years), serotiny was nearly universally uncommon (< 10%). Finally, forest structure strongly predicted squirrel density independently of serotiny, and serotiny provided no additional explanatory power, suggesting that the correlation is caused by selection against serotiny exerted by squirrels, rather than squirrels responding to variation in percentage serotiny.

Dysmyelination of Auditory Afferent Axons Increases the Jitter of Action Potential Timing during High-Frequency Firing

Auditory neuropathies are linked to loss of temporal acuity of sound-evoked signals, which may be related to myelin loss. However, it is not known how myelin loss affects the waveform and temporal precision of action potentials (APs) in auditory CNS nerve terminals. Here we investigated the excitability of the calyx of Held nerve terminal in dysmyelinated auditory brainstems using the Long-Evans Shaker (LES) rat, a spontaneous mutant where compact myelin wrapping does not occur due to a genetic deletion of myelin basic protein. We found at relatively mature postnatal ages (15-17 d after birth) LES rat calyces showed prolonged spike latencies, indicative of a threefold reduction in the AP propagation velocity. Furthermore, LES rat afferent fiber-evoked APs showed a pronounced loss of temporal precision, even at low stimulation frequencies (10 Hz). While normal calyces were able to fire APs without failures at impressive rates of up to 1 kHz, LES calyces were unable to do so. Direct recordings of the presynaptic calyx terminal AP waveform revealed that myelin loss does not affect the AP spike upstroke and downstroke kinetics, but dysmyelination reduces the after-depolarization and enhances the fast after-hyperpolarization peak following the AP spike in the LES rat. Together these findings show that proper myelination is essential not only for fast AP propagation, but also for precise presynaptic AP firing that minimizes both spike jitter and failures, two characteristics critically important for the accurate processing of sound signals in the auditory brainstem.

Rapid feedback regulation of synaptic efficacy during high-frequency activity at theDrosophilalarval neuromuscular junction

High-frequency firing of neurons depresses transmitter release at many synapses. At the glutamatergic synapse of the Drosophila larval neuromuscular junction, we find that presynaptic depression is modulated by postsynaptic ionotropic glutamate receptor (iGluR) activity. Although basal release at low frequency was insensitive to postsynaptic iGluR activity, recovery from depression elicited by high-frequency presynaptic trains decreased with partial block of native iGluRs. Moreover, recovery from depression increased with optical activation of the light-gated mammalian iGluR6 (LiGluR) expressed postsynaptically. The enhancement of recovery from depression occurred within 2 min of optical activation of LiGluR and persisted for minutes after optical deactivation. This effect depended on cAMP-dependent presynaptic recruitment of vesicles from the reserve pool. Our findings reveal a unique dimension to postsynaptic iGluR activity: fast retrograde signaling that preserves transmission efficacy during high-frequency presynaptic firing.

Repeated burning of eastern tallgrass prairie increases richness and diversity, stabilizing late successional vegetation

Understanding temporal effects of fire frequency on plant species diversity and vegetation structure is critical for managing tallgrass prairie (TGP), which occupies a mid-continental longitudinal precipitation and productivity gradient. Eastern TGP has contributed little information toward understanding whether vegetation-fire interactions are uniform or change across this biome. We resampled 34 fire-managed mid- and late-successional ungrazed TGP remnants occurring across a dry to wet-mesic moisture gradient in the Chicago region of Illinois, USA. We compared hypotheses that burning acts either as a stabilizing force or causes change in diversity and structure, depending upon fire frequency and successional stage. Based on western TGP, we expected a unimodal species richness distribution across a cover-productivity gradient, variable functional group responses to fire frequency, and a negative relationship between fire frequency and species richness. Species diversity was unimodal across the cover gradient and was more strongly humpbacked in stands with greater fire frequency. In support of a stabilizing hypothesis, temporal similarity of late-successional vegetation had a logarithmic relationship with increasing fire frequency, while richness and evenness remained stable. Temporal similarity within mid-successional stands was not correlated with fire frequency, while richness increased and evenness decreased over time. Functional group responses to fire frequency were variable. Summer forb richness increased under high fire frequency, while C4 grasses, spring forbs, and nitrogen-fixing species decreased with fire exclusion. On mesic and wet-mesic sites, vegetation structure measured by the ratio of woody to graminoid species was negatively correlated with abundance of forbs and with fire frequency. Our findings that species richness responds unimodally to an environmental-productivity gradient, and that fire exclusion increases woody vegetation and leads to loss of C4 and N-fixing species, suggest that these processes are uniform across the TGP biome and not affected by its rainfall-productivity gradient. However, increasing fire frequency in eastern TGP appears to increase richness of summer forbs and stabilize late-successional vegetation in the absence of grazing, and these processes may differ across the longitudinal axis of TGP. Managing species diversity in ungrazed eastern TGP may be dependent upon high fire frequency that removes woody vegetation and prevents biomass accumulation.

Coordinated Development of Voltage-Gated Na+and K+Currents Regulates Functional Maturation of Forebrain Neurons Derived from Human Induced Pluripotent Stem Cells

Like embryonic stem (ES) cells, human induced pluripotent stem (hiPS) cells can differentiate into neuronal cells. However, it is unclear how their exquisite neuronal function is electrophysiologically coordinated during differentiation and whether they are functionally identical to human ES cell-derived neurons. In this study, we differentiated hiPS and ES cells into pyramidal-like neurons and conducted electrophysiological characterization over the 4-week terminal differentiation period. The human neuron-like cells express forebrain pyramidal cell markers NeuN, neurofilament, the microtubule-associated protein 2 (MAP2), the paired box protein Pax-6 (PAX6), Tuj1, and the forkhead box protein G1 (FoxG1). The size of developing neurons increased continuously during the 4-week culture, and cell-resting membrane potentials (RMPs) underwent a negative shift from -40 to -70 mV. Expression of the muscarinic receptor-modulated K(+) currents (IM) participated in the development of cell RMPs and controlled excitability. Immature neurons at week 1 could only fire abortive action potentials (APs) and the frequency of AP firing progressively increased with neuronal maturation. Interestingly, the developmental change of voltage-gated Na(+) current (INa) did not correlate with the change in the AP firing frequency. On the other hand, the transient outward K(+) current (IA), but not the delayed rectifier current (IK) contributed to the high frequency firing of APs. Synaptic activities were observed throughout the 4-week development. These morphological and electrophysiological features were almost identical between iPS and ES cell-derived neurons. This is the first systematic investigation showing functional evidence that hiPS cell-derived neurons possess similar neuronal activities as ES cell-derived neurons. These data support that iPS cell-derived neural progenitor cells have the potential for replacing lost neurons in cell-based therapy.

Late and postglacial vegetation and fire history from Cordón Serrucho Norte, northern Patagonia

Pollen, macrofossil and charcoal analyses of a peat record from the Cordón Serrucho Norte mountain range east of the Andes in northern Patagonia (41°46′5″S, 71°25′13″W) provide vegetation and fire histories for the last 17,500calyrBP. The site is very distinctive as two rainforest conifers, Fitzroya cupressoides and Pilgerodendron uviferum, grow on the wetland with the more xeric Austrocedrus chilensis on the adjacent slopes, posing the question of how far back in time these Cupressaceae taxa co-occurred at the site. Late glacial regional vegetation changed from a pioneer herbaceous steppe to grass-dominated steppe at 16,500calyrBP and to closed Nothofagus forest by 15,200calyrBP, reflecting a two-step increase in precipitation. A shift to open forest and high fire frequency between 11,200 and 7000calyrBP is a consistent feature throughout northern Patagonia, indicating variable precipitation and seasonal drought. Between 7000 and 3000calyrBP, Nothofagus forest expanded again, fire frequency decreased and a Sphagnum magellanicum bog developed, replacing the Cyperaceae wetland, indicating year-round moisture. Present-day mixed Nothofagus dombeyi–A. chilensis forest developed after 3000calyrBP when fire frequency was again high. High precipitation variability in the late Holocene is most likely related to the onset of strengthening of ENSO. Our data, including stomata and wood analyses, do not unequivocally identify the age of co-existence of the three Cupressaceae taxa in the region. However, inferred moisture conditions, combined with genetic and dendroclimatic studies on Fitzroya and Pilgerodendron, suggest that this wetland site could have offered suitable habitat for these rainforest taxa during the last 17,500years, while the initial steppe environments and surrounding rocky and steep slopes could have provided adequate conditions for Austrocedrus.

Response of grass species to different fire frequencies in semi-arid rangelands of central Argentina

The study was undertaken to quantify the effect of different controlled fire frequencies on foliar cover, density, individual basal area, and mortality of the most common perennial grass species in the semi-arid rangelands of the southern Caldenal in central Argentina over a 20-year period. Cover of bare soil was also assessed. The study comprised three fire treatments: (i) high fire frequency (controlled burns every 3–5 years; HFF); (ii) low fire frequency (controlled burns every 8 years; LFF); and (iii) unburned control (C). Fire treatments, regardless of frequency, induced an increase in foliar cover and density in desirable grasses, no changes in intermediate grasses, and a decrease in undesirable grasses. Individual basal area tended to be higher for desirable grasses and lower for intermediate and undesirable grasses when subject to fire. Most of the species under study exhibited higher mortality rates in the HFF treatment than in the LFF and C treatments. The results of the study suggest that recurrent controlled burns of moderate intensity may favour the herbage production of desirable perennial grasses. This, in turn, assuming appropriate grazing management, may have a beneficial impact on livestock production. Nevertheless, given the effects of fire on the cover of bare soil and mortality of grasses, further research is needed in order to determine the appropriate fire frequency in terms of rangeland sustainability.

Effects of SK Channel Blockers on Atrial Myocytes Suggest SK Channel Heterogeneity

Atrial fibrillation (AF) is the most common sustained arrhythmia and contributes to cardiac morbidity and mortality. Extension of the atrial effective refractory period through block of potassium channels is a therapeutic strategy for AF, with an atrial-selective drug being desired to avoid potentially lethal ventricular arrhythmias. Small-conductance calcium-activated potassium (SK) channels have been recently suggested as a promising atrial selective target, but disagreement exists concerning expression and function of these channels in atrial myocytes. Visualization of antibody labelling using confocal microscopy showed the presence of SK2 protein in mouse atrial myocytes with localization of staining along the z-lines. Whole-cell recordings revealed outward currents positive to −20 mV that were sensitive to two SK inhibitors, apamin and UCL1684. Current was blocked by apamin with an IC50 of 118 pM, close to reported values for homomeric SK2 current in mammalian cell lines. Action potential duration (APD) was prolonged more by application of UCL1684 than apamin, at a firing frequency of 0.2 Hz. The effect of UCL1684 was greater with a firing frequency of 2 Hz, producing a decrease of the stability of APD and increasing beat-to-beat variability (BVR) in APD. These data suggest that functional SK channels are present in the mouse atrium. However, the effects of apamin were different under voltage- or current-clamp conditions, while the effects of UCL1684 were similar. This difference might arise from atrial myocytes expressing more than one population of SK channels, with one being apamin-insensitive and contributing to action potential repolarization. The effect of UCL1684 on APD is consistent with the recruitment of more SK channel activity at higher firing frequencies. Block of these SK channels increases BVR, a marker of drug induced repolarization-related proarrhythmias, raising the possibility that SK inhibition could be proarrhythmic.

A loss of hippocampal perineuronal nets produces deficits in dopamine system function: relevance to the positive symptoms of schizophrenia

Deficits in parvalbumin containing interneurons are a consistent observation in animal models and schizophrenia patients. These neurons are surrounded by chondroitin sulfate proteoglycans, forming perineuronal nets, thought to support the high firing frequencies observed in these neurons. A loss of perineuronal nets has been observed post mortem in human schizophrenia patients, however, whether this contributes to the symptoms of schizophrenia is not known. Here we directly examine the effects of chondroitinase ABC degradation of ventral hippocampal (vHipp) perineuronal nets, and demonstrate that this results in an enhanced hippocampal activity and significant increase in dopamine neuron population activity. In addition, chondroitinase-treated rats display an augmented locomotor response to amphetamine, consistent with the enhanced response to psychomotor stimulants observed in schizophrenia patients. Taken together, these data demonstrate that a loss of vHipp perineuronal nets is sufficient, in and of itself, to induce aberrant hippocampal and dopamine system function consistent with that observed in rodent models and schizophrenia patients.

Fire Detection Technologies Based on Video Image

Because of high fire frequency and huge losses, the research of fire signal detection in the monitoring system is an important task in the fire-preventing field. The fire signal detection method based on vision can overcome the shortcomings that exist in some traditional methods i.e. it can surmount the large impact on environmental interference factors, such as temperature, photographic and smoke of environment. With many researcher’s results, it shows clearly that the error rate of flame recognition is low, and also the real-time ability and the anti-disturbance ability are very good.

Mutagenesis Studies Investigating Slow Inactivation of Sodium Channel Nav1.6

Slow inactivation (SI) determines the availability of voltage-gated sodium channels and effects action potential firing patterns. Neuronally-expressed Nav1.6 was suggested to play an important role in maintaining high firing frequencies, possibly due to a reduced SI compared to other sodium channel subtypes. With this study we characterized SI of WT Nav1.6R and introduced mutations that severely altered the channel's propensity to slow inactivate. Conserved residues in the S6 segment of DII and DIII were identified as SI-sensitive hot spots: V966C in DII and N1455A in DIII. WT and mutant channels were transiently expressed in ND7/23 cells and patch-clamped. The N1455A mutation greatly enhanced SI whereas the V966C mutation shifted its voltage-dependence in the depolarizing direction by ∼25 mV. Fast inactivation was shifted slightly to more hyperpolarized potentials (∼3 mV for V966C and ∼8.5 mV for N1455A). We applied trains of depolarizing stimuli to imitate high-frequency firing. At a stimulation frequency of 50 and 100 Hz, only the N1455A mutation displayed enhanced run down, whereas V966C behaved similar to WT, probably due to the slight enhancement of fast inactivation of V966C. Our findings show that Nav1.6 SI can be severely altered by mutations in DII and DIII S6 segments, thereby confirming the importance of these regions to SI. N1455A induces loss of Nav1.6 during prolonged high frequency stimulation, which will allow us to test for the specific role of Nav1.6 vs. Nav1.7 in action potential (AP) generation: when transfected into DRGs, N1455A may attenuate prolonged high-frequency firing, whereas the threshold (thought to be set by Nav1.7) may remain unaffected. With this mutations we produced a useful molecular tool and approach for elucidating the role and influence of Nav1.6 SI on firing patterns in sensory neurons.

The robustness of phase-locking in neurons with dendro-dendritic electrical coupling

We examine the effects of dendritic filtering on the existence, stability, and robustness of phase-locked states to heterogeneity and noise in a pair of electrically coupled ball-and-stick neurons with passive dendrites. We use the theory of weakly coupled oscillators and analytically derived filtering properties of the dendritic coupling to systematically explore how the electrotonic length and diameter of dendrites can alter phase-locking. In the case of a fixed value of the coupling conductance (gc) taken from the literature, we find that repeated exchanges in stability between the synchronous and anti-phase states can occur as the electrical coupling becomes more distally located on the dendrites. However, the robustness of the phase-locked states in this case decreases rapidly towards zero as the distance between the electrical coupling and the somata increases. Published estimates of gc are calculated from the experimentally measured coupling coefficient (CC) based on a single-compartment description of a neuron, and therefore may be severe underestimates of gc. With this in mind, we re-examine the stability and robustness of phase-locking using a fixed value of CC, which imposes a limit on the maximum distance the electrical coupling can be located away from the somata. In this case, although the phase-locked states remain robust over the entire range of possible coupling locations, no exchanges in stability with changing coupling position are observed except for a single exchange that occurs in the case of a high somatic firing frequency and a large dendritic radius. Thus, our analysis suggests that multiple exchanges in stability with changing coupling location are unlikely to be observed in real neural systems.