Higher Fat Levels Research Articles

β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model.

Non-alcoholic fatty liver disease (NAFLD) is strongly associated with hyperlipidemia, which is closely related to high levels of sugar and fat. β-sitosterol is a natural product with significant hypolipidemic and cholesterol-lowering effects. However, the underlying mechanism of its action on aquatic products is not completely understood. A high-fat diet (HFD)-induced NAFLD zebrafish model was successfully established, and the anti-hyperlipidemic effect and potential mechanism of β-sitosterol were studied using oil red O staining, filipin staining, and lipid metabolomics. β-sitosterol significantly reduced the accumulation of triglyceride, glucose, and cholesterol in the zebrafish model. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differential lipid molecules in β-sitosterol mainly regulated the lipid metabolism and signal transduction function of the zebrafish model. β-sitosterol mainly affected steroid biosynthesis and steroid hormone biosynthesis in the zebrafish model. Compared with the HFD group, the addition of 500 mg/100 g of β-sitosterol significantly inhibited the expression of Ppar-γ and Rxr-α in the zebrafish model by at least 50% and 25%, respectively. β-sitosterol can reduce lipid accumulation in the zebrafish model of NAFLD by regulating lipid metabolism and signal transduction and inhibiting adipogenesis and lipid storage.

Consumer Behavior of School-Going Children Towards Fast Food Items in New Delhi

Amongst all modifiable risk factors for nutrition-related health problems in school-aged children are dietary choices. Fast food culture is becoming popular among school-aged children. It is highly convenient and tasty, and youngsters enjoy it due to peer pressure. However, understanding how students are drawn to fast foods has become increasingly important in recent years. The purpose of this study is to learn about the fast-food consumption patterns and preferences of school-aged children in New Delhi. In this study, a self-designed and pretested questionnaire was used to conduct the research. The acquired raw data was subjected to appropriate statistical analysis in order to produce relevant results. The results showed that 15% of participants do not have a preference for fast food, compared to the majority of children (85.25%) who are more likely to prefer eating fast food. According to the percentage of school-age children who eat fast food, 22% of them eat pizza/week, 16% of them eat pasta twice weekly, and 27% of them eat Maggi once or twice a week. In contrast, only 12% of them eat wraps weekly, 12% of them eat wafers, 17% of them eat cookies twice weekly, and 32% of them eat doughnuts infrequently. Fast food contains high levels of unhealthy sugars and fats that are also addictive, which makes it difficult for kids to make good eating choices.

“Unlocking the Therapeutic Potential: A Comprehensive Review of the Pharmacological Activities of Shiitake Mushroom (Lentinula edodes)"

Abstract: Worldwide, edible mushrooms are prized for their medical and nutraceutical qualities. Because of their high protein content, low fat and cholesterol levels, low-calorie content, and abundance of vital vitamins, they are acknowledged as the new superfood of the future. This is considered that mushrooms are the only vegetarian source of vitamin D because they contain high amount of ergosterol and fat-soluble vitamins. Limited information exists regarding the composition and amount of fiber, although polysaccharides such as β-glucans increase the nutritional value of mushrooms. Mushrooms are a fantastic food because of their many pharmacological and nutraceutical qualities, as has been noted. Shiitake mushrooms are nutrient-dense, widely farmed fungal plants and possess various pharmacological and nutraceutical properties. The purpose of this review is to compile the most recent information on the health advantages and medicinal uses of shiitake mushrooms.

The effects of metabolic syndrome on arterial stiffness: A concise review.

Madam, The term "metabolic syndrome" describes a collection of diseases that escalates a person’s risk of cardiovascular disease, stroke, adult-onset diabetes, and other severe health issues. It is also known as Insulin resistance syndrome. A major cause of metabolic syndrome is the development of resistance to insulin due to high levels of fats. (1) Multiple risk factors, including abdominal and central obesity, increased blood pressure, low amount of HDL, and higher amount of LDL and triacylglycerides characterize it. Metabolic syndrome and arterial stiffness are interconnected conditions that can significantly impact cardiovascular health. (2) Arterial stiffness is a condition that considers the rigidity of the arterial wall. It involves modification of the automated aspects of the walls of arteries. The macroscopic geometry as well as the intrinsic characteristics of the artery, play a role in its stiffness. (3) Several mechanisms lead to arterial stiffness in metabolic syndrome patients, the first being inflammation. Upon excess nutrition, adipocytes increase in size and number, which causes decreased perfusion, causing hypoxia. Cell necrosis with the invasion of macrophages and the synthesis of adipocytokines, which consist of the pro-inflammatory mediators, might result from this condition. (1) The increased vascular inflammation will promote vascular fibrosis, smooth muscle cell proliferation, and endothelial-mediated vasodilation, which will ultimately increase arterial stiffness. (4) Metabolic syndrome is also related to insulin resistance, lipidemia, and central obesity. Chronic hyperglycemia and hyperinsulinemia may enhance sympathetic nervous system activity, vascular inflammation, and renin-angiotensin system activity. Vascular tissue's angiotensin-aldosterone system results in fibrosis and wall enlargement. Middle-aged people transitioning from gaining body weight to established conditions like hypertension and type 2 diabetes experience arterial stiffness. This transition typically starts with a few minor changes, but Metabolic Syndrome develops when several risk factors are present. (5) In conclusion, there is evidence to suggest arterial stiffness and metabolic syndrome are closely related and have significant implications for the well-being of an individual. It is essential to address both conditions as they may lead to atherosclerosis, followed by more frequent cardiovascular events and higher mortality. There are various strategies to counter the rise of arterial stiffness, like adding exercises to routines and vitamin K-rich foods to the diet, which will in turn reduce the occurrence of metabolic syndrome disorders in individuals, thus leading to a healthier society.

Virgin flaxseed oil supplemented or not with tocopherols and phytosterols prevents liver steatosis induced by high‐fat diets in male Wistar rats

AbstractThis study aimed to investigate the effect of a virgin flaxseed oil (FO) rich in n − 3 polyunsaturated fatty acid, supplemented or not with a bioactive supplement (BS) containing tocopherols and phytosterols on the prevention of nonalcoholic fatty liver disease in a high‐fat (HF) animal model. Male Wistar rats were fed 60 days with a recommended (SO7, soybean oil 7%) or an HF diet (fat, 30%) containing soybean (SO30), corn (CO30), or FO (FO30) or the same oils supplemented with 1% of BS (SO30BS, CO30BS, or FO30BS, respectively). Hepatic and dietary FA composition, triacylglycerides (TAGs) content in serum and liver, nutritional parameters, in vivo TAG‐secretion rate, liver histology, mRNA levels of β‐oxidation‐related genes, and carnitine palmitoyl transferase‐1a (CPT‐1a) enzyme activity were determined. At HF levels, FO30 and FO30BS groups showed the highest total n − 3 PUFA levels in diets and were associated with lower weight gain, liver TAG content, and microsteatosis degree. The CPT‐1a activity was enhanced in FO30 and FO30BS, showing differences only by the type of dietary oil. The BS supplementation induced an increase in the β‐oxidation‐related gene expression. Virgin FO prevented liver steatosis and hepatomegaly induced by HF diets. The BS supplementation reduced body fat accumulation and enhanced expression of β‐oxidation related genes, without affecting hepatic TAG content.Practical Applications: Scientific knowledge of functional lipids with high oxidative stability and biological activity has great practical application in reducing non‐communicable chronic diseases. This study provides evidence on the potential synergic and complementary effects of virgin FO, TP, and PS to be used for the prevention of the metabolic hepatic disorders involved during the evolution of the different steps of NAFLD. Specifically, based on different biological mechanisms, this stable combination of bioactive lipids could prevent not only the development of exacerbated hepatic TAG accumulation but also the hepatocellular injury seen at different stages from hepatic steatosis to steatohepatitis.

The Effect of Water Stress on Bioactive Compounds in Australian-Grown Black Sesame

Sesame is an emerging crop of interest in Australia and has attracted widespread interest due to the health-benefitting properties of its bioactive compounds, including fatty acids, lignans, and polyphenols. This study aimed to investigate the impact of drought stress on these bioactive compounds, using eleven cultivars of black sesame seeds grown in Australia. Specific varieties responded positively to water deficit (WD) conditions, showing increased levels of TPC, FRAP, CUPRAC, and lignans. Varieties 1, 4, 7, and 12 showed significantly increased FRAP values ranging from 158.02 ± 10.43 to 195.22 ± 9.63 mg TE/100 g DW in the WD treatment compared to the well-watered (WW) treatment, whereas varieties 7, 10, 12, 13, and 18 demonstrated the highest CUPRAC values of all varieties (2584.86 ± 99.68–2969.56 ± 159.72 mg TE/100 g) across both WW and WD conditions, with no significant variations between irrigation regimes. Moreover, lignan contents (sesamin and sesamolin) were higher in varieties 1, 2, 5, and 8 grown in WD conditions. Compared to the optimal unsaturated to saturated fatty acid ratio (Σ UFA/Σ SFA ratio) of 0.45, all sesame genotypes showed superior ratios (ranging between 1.86 and 2.34). Moreover, the ω-6/ω-3 PUFA ratio varied from 33.7–65.5, with lower ratios in varieties 2, 4, 5, 8, and 18 under WD conditions. The high levels of phenolic compounds and healthy fats suggest the potential of black sesame to be incorporated into diets as a functional food. Furthermore, the enhanced phytochemistry of these cultivars in WD conditions is promising for widespread adoption. However, larger trial studies to confirm these findings across different geographic locations and seasons are warranted.

Nutritional values of ready-to-eat snacks available in the Indian E-market—a comparative study based on the health star rating system

BackgroundThe popularity of ready-to-eat (RTE) foods has increased globally, especially among children, because they are readily available, convenient, tasty, and have attractive packaging designs. The number of packaged foods, drinks, and snacks has risen in the Indian market, and the presence of less-healthy packaged products is a significant cause of overweight and diseases related to diet. The Health Star Rating (HSR) System, initiated by the Australian and New Zealand governments, was designed to rank these packaged products based on fat, sugar, energy, salt, and other nutritional compositions. The study assessed the nutritional value of RTE snacks currently available in the Indian e-market using the HSR system.MethodsThis comparative study analyzed the nutritional values of RTE snacks from November 2022 to February 2023. The data were extracted from the Indian e-market, namely Amazon Fresh, Flipkart Grocery, and Big Basket, using specific keywords. The HSR system has been used to evaluate and compare the healthiness of RTE snacks, using a star rating scale of 0.5 stars = bad to 5 stars = excellent. The study assessed 294 RTE products, including chips, cocoa-based snacks, and baked snacks, with a price range of INR 50 and below.ResultsThe findings revealed that the mean HSR was low (1.46) stars [SD 0.63] out of (5) stars for the studied products, with significant concerns about high levels of energy, sugar, sodium, and saturated fat. Additionally, the study observed a limited utilization of fruits, vegetables, nuts, and legumes (FVNL) in the market products.ConclusionThe study highlights the unhealthiness of snack products available in the Indian e-market due to high levels of sugar, salt, and fat. These snacks also lack FVNL as nutritious ingredients. The data extracted can underscore public health concerns, highlight the importance of consumer awareness, and help snack manufacturers develop healthier snacks for society.

Dietary fat and high energy density diet: Influence on intestinal health, oxidative stressand performance of weaned piglets.

The utilization of dietary components to support gut function and the health of young animals is an important factor for improved performance. The influence of high dietary fat levels in a lowor high energy density diet on the performance of weaned piglets in relation to intestinal absorptive function, amino acid utilization, oxidative stress, and microbial metabolites was assessed in this study. The study examined the effect of two different diets containing either a low energy density/high-fat level or a high energy density/high-fat level. A total of 16 healthy weaners (9.60 ± 0.13 kg) were allocated to one of the two dietary treatments. There were eight weaners per treatment. Results showed that feed intake and body weight gain of weaners were increased by the diet of high energy density/high-fat level (p < 0.05), but the feed efficiency showed an increased tendency of significance (p = 0.05). In the duodenum, the villus height(VH) and VH/crypt depth (CD) ratio (VH:CD) were increased by dietary high energy density/high fat. In the jejunum and ileum, the CD was increased by low energy density/high-fat diet, while the goblet cell count and VH:CD were increased by dietary high energy density/high-fat level. Methionine, lysineand phenylalanine concentrations were increased by high energy density/high-fat diet while low energy density/high-fat diet showed an increased tendency to increase citrulline and ornithine concentrations in the piglet. Oxidative stress marker, lactase enzyme activity and serum calcium concentration were increased by a high energy density/high-fat diet. Increased dietary fat in all diets induced diarrhoea in the weaners (p < 0.01). It was concluded that a dietary high energy density/high-fat diet seems to positively modulate gut absorptive function, serum amino acid (methionine and lysine), calcium levels and increased oxidative stress markers in the weaned piglets.

Exploring the impact of culture media on life history characteristics of the house fly, Musca domestica L. (Diptera: Muscidae)

Insects, particularly house fly pupae (M. domestica L.), are increasingly promoted as a rich and sustainable protein source, presenting a practical solution for organic waste management. These insects possess the capability to transform animal waste into valuable biomass, underscoring the necessity to evaluate housefly larval growth on diverse waste mixtures. The primary objective was to comprehend how different culture media influence the life history traits of this species. To address this, the research aimed to assess the growth and development performance of housefly larvae reared on various waste mixtures. Adult house flies (M. domestica) were sourced from meat and fish markets in Rajshahi city, Bangladesh, employing systematic sampling from different market sections for a representative selection. Experimental diets were formulated with combinations of cattle blood, cattle manure, rice bran, wheat bran, and kitchen waste, categorized into Diet-A (kitchen waste:cattle manure:wheat bran), Diet-B (cattle blood:rice bran), Diet-C (cattle manure:wheat bran), and Diet-D (cattle blood:kitchen waste:rice bran). Additionally, a control group comprised a mixture of waste materials, including yeast, egg white, milk powder, and wheat bran. The findings revealed that larvae, pupae, and adults reared on Diet-B, incorporating cattle blood and rice bran, demonstrated increased length compared to other diets. This varied diet also produced the heaviest larvae, pupae, and adults, exhibiting the highest morphometric parameters. The moisture content of pupae varied across the different diets, ranging from 53.23±0.17 % to 65.47±1.32 %. The pupae reared on Diet-B had the highest levels of crude protein (53.44±0.05 %, p < 0.05), while those reared on Diet-C had the highest levels of fat (18.56±0.05 %, p < 0.05 except for diet A). The findings demonstrate that housefly larvae can be reared on diets containing rice bran and cattle blood, which have different nutritional profiles and biological capacities. However, the ideal rearing strategy should depend on how M. domestica will be utilized in the future. The study highlights insects, especially housefly pupae, as a sustainable protein source and an organic waste management solution. Therefore, the results of this study contribute to the broader understanding of Musca domestica and offer practical implications for both scientific research and pest management practices.

TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition

The Laiwu pig is an indigenous fatty pig breed distributed in North China, characterized by an extremely high level of intramuscular fat (IMF) content (9% ∼ 12%), but the regulatory mechanism underlying intramuscular fat deposition in skeletal muscle is still unknown. In this study, the TMT-labeled quantitative malonylome of the longissimus dorsi muscle in Laiwu pigs at the fastest IMF deposition stage (240 d vs 120 d) was compared to analyze the molecular mechanism of IMF variation in pigs. In Laiwu pigs aged 240 days/120 days, we identified 291 malonylated lysine sites across 188 proteins in the longissimus dorsi muscle. Among these, 38 sites across 31 proteins exhibited differential malonylation. Annotation analysis and enrichment analysis were performed for differentially malonylated proteins (DMPs). These DMPs were mainly clustered into 12 GO functional categories accounting for 5 biological processes, 4 cellular components and 3 molecular functions, and 2 signaling pathways by KEGG enrichment analysis. The function of differentially malonylated protein ACOT7 in the process of fat deposition was further investigated during the differentiation of 3 T3-L1 cells. The results showed that the protein level of ACOT7 in 3 T3-L1 cells decreased but the malonylated level of ACOT7 increased significantly. The malonyl-CoA that is synthesized by ACSF3 affected the malonylation level of ACOT7 in 3 T3-L1 cells. SignificanceThe intramuscular fat (IMF) content, by affecting sensory quality traits of meat, such as tenderness, flavor and juiciness, plays an important role in meat quality. Using TMT-based quantitative malonylated proteome analysis, we identified malonylated proteins in LD muscle samples in two stages (120 d and 240 d) of development and further identified differentially malonylated proteins, such as SLC25A4, ANXA5, TPM3 and ACOT7, that are associated with intramuscular fat deposition and fat metabolism in pigs. These differentially malonylated proteins could serve as candidates for elucidating the molecular mechanism of IMF deposition in pigs. In addition, we found that the malonyl-CoA in 3 T3-L1 cells is mainly synthesized by ACSF3, affecting the malonylated level of ACOT7. The study provides some data concerning the role of protein malonylation in regulating the variation in porcine IMF content.

Enhancing ice cream qualities with novel rice husk cellulose nanocrystal applications

SummaryCellulose nanocrystals derived from rice husk (CNC) using a chemical method combined with high‐pressure homogenisation, were investigated for their application in ice cream formulations. The physicochemical and sensory properties of ice cream with CNC at three different concentrations (0%, 0.15%, and 0.30% wt.) and two levels of whipping cream as fat content (5% and 10% wt.) were studied. The results indicate that increased CNC concentration enhanced the overrun and viscosity of the ice cream samples. Furthermore, 10% fat ice cream with 0.30% CNC exhibited higher total phenolic content and antioxidant activity compared to the others. The hardness and melting rate of the ice cream samples tended to decrease with higher fat levels and CNC concentrations. Moreover, CNC helped to improve the quality of low‐fat ice cream while still maintaining satisfactory sensory properties. Therefore, CNC has the potential as a stabiliser for ice cream to improve its overall properties.

Composites flour formulation made from yellow pumpkin, purple sweet potato, corn, and wolf-herring flour for replacement of wheat flour on low- and high- moisture foods part I: Cookies and muffin

IntroductionThe study investigated the potential of yellow pumpkin, purple sweet potato, corn, and wolf-herring flour as local-based ingredients in generating cookies and muffin rich in minerals and amino acids. MethodsFive different composites were prepared and applied for cookies and muffins preparation replacing wheat flour. Sensory acceptability was conducted, and selected formulas were further evaluated for their chemical composition including proximate, dietary fiber, minerals (Mg, P, Fe, Zn and Ca) and amino acids. Results and discussionA formula consisting of 45 g yellow pumpkin-, 45 g purple sweet potato-, 5 g corn- and 5 g wolf-herring flour was selected as the highest acceptability. Compared to muffins, cookies had a higher level of fat (21.6%), protein (8.6%), ash (2.9%), dietary fiber (14.4%), calorie (462.6 cal/100 g), Mg (47.9 mg/100 g), P (1725.9 mg/kg), Fe (2.0 mg/100 g), Zn (0.9 mg/100 g) and Ca (136.8 mg/100 g). Cookies and muffins contained 15 L-amino acids in which 7 were amino acids essentials. The higher amino acids were L-aspartic acid and L-glutamat acids ranged between 6.9 – 8.1 and 8.9 – 9.5 g/kg, respectively. The lowest amino acids obtained was L-Histidine at 1.9 g/kg for cookies and muffin. ConclusionIn conclusion, cookies, and muffin rich in mineral and amino acids can be formulated based on low-cost locally available food ingredients.

The Influence of Ultra-Processed Food on Colorectal Cancer: A Systematic Review

Colorectal cancer (CRC) is a disease characterised by the development of malignant tumours in the colon or rectum; it is considered the most common cancer in women, and up to 50% of cases can be prevented with a healthy lifestyle. Nutritional habits are related to its appearance, and the current trend of an increased consumption of ultra-processed foods (UPFs) has led to a surge in the incidence of CRC in recent years. This systematic review aims to evaluate, based on scientific evidence, the role of UPF in the incidence of CRC. The PubMed, Scopus, CINAHL, and Web of Science databases were reviewed, and a total of 24 scientific articles were selected according to the inclusion and exclusion criteria of this review (studies from the past 5 years and observational studies in English). The conclusions of this study point to an association between UPF-based diets and the appearance of CRC, which is promoted by the harmful effects of the consumption of high levels of sugar, fat, red meat, and additives. These dietary habits, coupled with a sedentary lifestyle and obesity, further increase the incidence of CRC.

Pelatihan Oleh Fasilitator Sebaya Menggunakan Aplikasi Health Heroes Nutrihunt Untuk Memilih Makanan Minuman Sehat Dan Meningkatkan Pengetahuan Remaja Dalam Mencegah Masalah Gizi Remaja

The problem of excess or deficiency of nutrients in adolescents will increase susceptibility to disease, especially the occurrence of non-communicable diseases such as hypertension, diabetes, coronary heart disease, stroke, and cancer, if not addressed immediately. Limiting eating and drinking that contain high levels of sugar, salt, and fat is a preventive measure for non-communicable diseases in adolescents. The purpose of community service is to increase adolescents' knowledge about preventing and overcoming nutritional problems. The community service method is to provide training to 100 adolescents of SMAN 1 Banyuwangi through material presentation and practice on adolescent health and nutrition issues, adolescent nutrition problems and how to overcome them, and how to choose healthy food and drinks according to adolescent needs with the Health Heroes Nutrihunt application. The analysis used to determine the results of the training is the pre-test and post-test. The results of this service show that there is an increase in adolescents' knowledge about the topic of exposure, as seen from the results of the pre-test and post-test, which have an average of 46.23 and 72.96, respectively, which shows an increase of 57.8% and can be seen from the enthusiasm to practice identifying types of packaged food and drinks that are good for consumption. With this training, adolescents in schools are able to sort and choose foods and drinks before consumption based on their nutritional content.

Application of Lipid Apheresis in Acute Lipogenic Pancreatitis

Plain Language SummaryHypertriglyceridemia-associated pancreatitis is a condition where high levels of fats in the blood can lead to pancreas inflammation. The treatment goal is to quickly lower these fat levels. Lipid purification, a method to remove fats from the blood, has shown promise but lacks clear guidelines for its use. This study aimed to analyze the data of patients with this type of pancreatitis who underwent lipid purification to determine its effectiveness. Five patients diagnosed with acute lipogenic pancreatitis were included in the study. They received routine therapies and underwent lipid purification using a technique called DFPP. After a single treatment, significant improvements were observed in the patients’ lipid levels. Triglyceride levels decreased by 80% on average, and total cholesterol decreased by 59%. Lipid purification effectively improved symptoms, reduced lipid levels.

Nutritional composition of ultra-processed plant-based foods in the out-of-home environment: a multi-country survey with plant-based burgers.

Ultra-processed plant-based foods, such as plant-based burgers, have gained in popularity. Particularly in the out-of-home (OOH) environment, evidence regarding their nutritional profile and environmental sustainability is still evolving. Plant-based burgers available at selected OOH sites were randomly sampled in Amsterdam, Copenhagen, Lisbon and London. Plant-based burgers (patty, bread and condiment) (n 41) were lab analysed for their energy, macronutrients, amino acids and minerals content per 100 g and serving and were compared with reference values. For the plant-based burgers, the median values per 100 g were 234 kcal, 20·8 g carbohydrates, 3·5 g dietary fibre and 12·0 g fat, including 0·08 g TFS and 2·2 g SFA. Protein content was 8·9 g/100 g, with low protein quality according to amino acid composition. Median Na content was 389 mg/100 g, equivalent to 1 g salt. Compared with references, the median serving provided 31% of energy intake based on a 2000 kcal per day and contributed to carbohydrates (17-28%), dietary fibre (42%), protein (40%), total fat (48%), SFA (26%) and Na (54%). One serving provided 15-23% of the reference values for Ca, K and Mg, while higher contributions were found for Zn, Mn, P and Fe (30-67%). The ultra-processed plant-based burgers provide protein, dietary fibre and essential minerals and contain relatively high levels of energy, Na and total fats. The amino acid composition indicated low protein quality. The multifaceted nutritional profile of plant-based burgers highlights the need for manufacturers to implement improvements to better support healthy dietary habits, including reducing energy, Na and total fats.

Phenotypic and metabolomic characteristics of mouse models of metabolic associated steatohepatitis

BackgroundMetabolic associated steatohepatitis (MASH) is metabolic disease that may progress to cirrhosis and hepatocellular carcinoma. Mouse models of diet-induced MASH, which is characterized by the high levels of fats, sugars, and cholesterol in diets, are commonly used in research. However, mouse models accurately reflecting the progression of MASH in humans remain to be established. Studies have explored the potential use of serological metabolites as biomarkers of MASH severity in relation to human MASH.MethodsWe performed a comparative analysis of three mouse models of diet-induced MASH in terms of phenotypic and metabolomic characteristics; MASH was induced using different diets: a high-fat diet; a Western diet; and a high-fat, high-cholesterol diet. Liver cirrhosis was diagnosed using standard clinical approaches (e.g., METAVIR score, hyaluronan level, and collagen deposition level). Mouse serum samples were subjected to nuclear magnetic resonance spectroscopy–based metabolomic profiling followed by bioinformatic analyses. Metabolomic analysis of a retrospective cohort of patients with hepatocellular carcinoma was performed; the corresponding cirrhosis scores were also evaluated.ResultsUsing clinically relevant quantitative diagnostic methods, the severity of MASH was evaluated. Regarding metabolomics, the number of lipoprotein metabolites increased with both diet and MASH progression. Notably, the levels of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) significantly increased with fibrosis progression. During the development of diet-induced MASH in mice, the strongest upregulation of expression was noted for VLDL receptor. Metabolomic analysis of a retrospective cohort of patients with cirrhosis indicated lipoproteins (e.g., VLDL and LDL) as predominant biomarkers of cirrhosis.ConclusionsOur findings provide insight into the pathophysiology and metabolomics of experimental MASH and its relevance to human MASH. The observed upregulation of lipoprotein expression reveals a feedforward mechanism for MASH development that may be targeted for the development of noninvasive diagnosis.

New supplementary feeds for sterlet in industrial cultivation

An increasingly significant deficit of fish meal necessary for the production of quality fish feed requires the use of new components characterised by high levels of protein and fat. This article presents the results of a feed supplements study with a protein component based on soldier fly and probiotics. The study of fish-biological parameters of fish revealed that the absolute and average daily gain in the variant with feed KV54 + soldier fly is higher by 40.9 %, the mass accumulation ratio by 33.3 %, compared with the control variant, with survival rate of 100 %. Feeding coefficient in the experiment variant KV54+soldier fly is lower compared to the control by 1.3 units, i.e. by 34 %. The physiological and biochemical parameters of fish blood were within the normal range for fish from a natural reservoir.

Pembuatan Tepung Telur Ayam (Gallus gallus. D) Asin Rebus

Chicken eggs are a food that is very rich in nutrients, including protein (12.8%) and fat (11.8%). The high levels of water, fat and protein in eggs can make them a good medium for bacterial growth. Quality deterioration in eggs can be prevented by using egg preservation methods, namely drying eggs into egg powder or flour. Chicken eggs are still very limited and inefficient processing to be used as salted egg flour. Therefore, it is necessary to conduct research on the manufacture of boiled salted chicken egg flour (Gallus gallus. D). This research was conducted to study the making of boiled salted chicken egg flour and understand the characteristics by taking measurements on whole eggs, egg whites, and yolks using the proximate test method, measurement of fineness, and color test. This research was conducted using a non-factorial Completely Randomized Design (CRD) model consisting of 6 testing factors (T) and (A), namely: T1 whole unsalted chicken eggs, T2 white unsalted chicken eggs, T3 yellow unsalted chicken eggs, A1 whole salted chicken eggs, A2 white salted chicken eggs, and A3 yellow salted chicken eggs. The research results of salted chicken egg flour making is a process that involves drying salted eggs and grinding them into flour form. The preparation of salted egg flour showed that the difference between regular chicken eggs and salted chicken eggs, which resulted, affected the proximate properties and had a noticeable impact on the nutritional composition and physical properties of the egg flour produced, while the moisture content was not significantly different. Keywords: Chicken Egg Flour, Egg Preservation, Proximate Properties, Salted Eggs.

Visceral adiposity associated with incidence and development trajectory of cardiometabolic diseases: A prospective cohort study

Background and aimsThere is a lack of literature concerning the effects of visceral adipose on the development of first cardiometabolic disease (FCMD) and its subsequent progression to cardiometabolic multimorbidity (CMM) and mortality. Methods and results423,934 participants from the UK Biobank with different baseline disease conditions were included in the analysis. CMM was defined as the simultaneous presence of coronary heart disease, T2D, and stroke. Visceral adiposity was estimated by calculating the visceral adiposity index (VAI). Multistate models were used to assess the effect of visceral adiposity on the development of CMM. During a median follow-up of 13.5 years, 50,589 patients had at least one CMD, 6131 were diagnosed with CMM, whereas 24,634 patients died. We observed distinct roles of VAI with respect to different disease transitions of CMM. HRs (95 % CIs) of high VAI were 2.35 (2.29–2.42) and 1.64 (1.50–1.79) for transitions from healthy to FCMD and from FCMD to CMM, and 0.97 (0.93–1.02) for all-cause mortality risk from healthy, FCMD and CMM, respectively. ConclusionsOur study provides the first evidence that visceral adipose may contribute to the development of FCMD and CMM in healthy participants. However, visceral adipose may confer resistance to all-cause mortality in participants with existing CMD or CMM. A better understanding of the relationship between visceral adipose and CMM can focalize further investigations on patients with CMD with high levels of visceral fat and help take targeted preventive measures to reduce the medical burden on individual patients and society.