Highest Extraction Yield Research Articles

Enhanced gelling performance of oxhide gelatin prepared from cowhide scrap by high pressure-assisted extraction.

In this study, the feasibility of preparing oxhide gelatin from cowhide scrap by high pressure assisted-liquid extraction was verified. Different processing conditions, including high pressure time (15 to 25 min), pressure (250 to 350 MPa), and liquid-to-solid ratio (1:3 to 1:5), were optimized through response surface methodology. Under the optimum manufacturing conditions, when the high-pressure processing (HPP) time was 22 min, the pressure was 289 MPa, and the liquid-to-solid ratio was 1:4, the highest extraction yield (36%) and gel strength (224 g) were achieved. Based on DSC, XRD, FTIR, SEM, gelling and melting temperatures, HPP led to the structural modification of the gelatinized collagen, which enhanced the rearrangement of the gel structure during the gelation process and made it have better gelling properties. In addition, compared with the commercial sample, they do not differ significantly in the relaxation time and peak area of prepared oxhide gelatin. These findings provide new insights into the practicability of HPP during the preparation of oxhide gelatin, which can noticeably reduce the processing time and be applied to industrial production. PRACTICAL APPLICATION: Compared with traditional processing, a high pressure-assisted extraction process can noticeably reduce the processing time while producing cowhide gelation with similar physicochemical and functional properties. Meanwhile, high pressure processing (HPP) led to the structural destruction of the cowhide and gelatinized collagen, which enhanced the rearrangement of the gelatin structure during the gelation process and made it have better gelling properties. Importantly, high pressure-assisted extraction can facilitate the use of a low-cost raw material and improve the preparation efficiency of oxhide gelatin, which shows great potential in large-scale and efficient industrial production and the quality control of oxhide gelatin.

Sustainable exploitation of macroalgae species from Chilean coast: Characterization and food applications

The use of macroalgae has been always linked to direct consumption, however, current trends have increased the applications of algae in the formulation of foods to replace animal proteins. Hence, we must ensure the safety of the increasing food products based on macroalgae. In this regard, and due to the worldwide variability of its composition, we determined the nutritional characterization of Durvillaea Antarctica, Macrocystis pyrifera, and Lessonia nigrescens to evaluate their potential at nutritional level. Also, as they are accumulating organisms, we characterized their minerals and heavy metals content, to determine its potential applications and consumption risks. The results indicated that the content total As is into the safe range to be consumed (16.92 and 60.75 mg/kg dw), but Cd (between 3.97 and 6.15 mg/kg dw) is higher than 0.5 mg/kg of final product (regarding Chilean Normative), and in this case, we have two possibilities: To add no more than 12.5% of M. pyrifera and no more than 8% of D. antarctica or L. nigrescens flour in the final product; or avoid the heavy metals by the extraction of alginates to enhance applications in food such as increasing the viscosity or improving dispersion stability. From the last point of view, we have compared the alginates extracted from D. antarctica, M. pyrifera, and L. nigrescens through the use of two methodologies to obtain calcium alginate (10.55–16.75% extraction yield) and sodium alginate (12.45–20.8% extraction yield), where the methodology to extract sodium alginate showed the best perform for the three macroalgae. The results showed that viscosities at 20 °C and alginate concentration of 40 g/L were: 1201.4 cP, 544.0 cP and 722.4 cP for D. antarctica, M. pyrifera, and L. nigrescens respectively. With these results we can conclude that the macroalgae D. antarctica is the one with both the highest extraction yield and technological potential as it generates a higher viscosity.

Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment

The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods. In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE). The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated. MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods. The smaller particle size of mulberry powder was found to improve the extraction yields. The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure. MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs. This study identified that HSEHE was the most suitable extraction method for MFP.

Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity.

Recently, natural resources have attracted considerable interest for their applications in food security and human health problems. Traditional natural spices, such as star anise and black pepper, played important roles in the pharmaceutical and food industries due to their strong pharmacological activity, antioxidant potential and rare complications. In order to achieve biomasses from the natural product with multiple bioactivities, we developed the multistage extraction method to extract and separate various bioactive compounds from these natural plants. Our work demonstrated that various bioactive-rich extractives were achieved using steam distilled- or oxidative-extraction methods with high extraction yields and purity. Furthermore, the extractives in each step can be used not only as bioactive compounds, but also as a resource to further prepare different derivatives during the next extractive step, providing biomass-saving to a great extent. The extractives obtained with high yields and purities (>82%) were identified by 1H NMR, 13C NMR, FTIR, UV-vis, fluorescence spectroscopy, and high-performance liquid chromatography (HPLC). Moreover, these biomasses display potent antibacterial activities against some types of microorganisms such as S.aureus, S.pyogenes, E.coli, and S.typhi with a lowest MIC of 400 μg/ml for the development of antibacterial agents, significant antioxidant activity as the natural antioxidant for enhancing food shelf-life, and excellent anticancer activity that induces significant cancer cell apoptosis. This work showed the different multistage extracts from natural products, which enable them to be applied in the fields of the pharmaceutical industry and the food industry.

Sequential multi-stage extraction of biocompounds from Spirulina platensis: Combined effect of ohmic heating and enzymatic treatment

A sequential multi-stage procedure was applied on the extraction of biocompounds from Spirulina platensis. The process consisted in three steps: 1) aqueous extraction, using conventional thermal extraction (CE), ohmic heating (OH, 7 V/cm), enzymatic treatment (EAE, 0.8 mgLysozyme/mL), or both OH and EAE combined; 2) ethanolic extraction; 3) CHCl3/MeOH extraction. The results evidenced that the combined OH-EAE extraction allowed selective recovery of phycobiliproteins in the 1st step, with increments of more than 100% in yield in comparison with CE. Pigments and lipids were selectively extracted in the 2nd step. The combination of OH and EAE in the 1st step resulted in higher amounts of extracted compounds in the following phases compared to processes using non-combined technologies. Results demonstrate that the intensification of extraction steps facilitates the use of environmentally friendly technologies in a multi-stage process capable of recovering and isolating different fractions with bio-functional properties, targeting waste reduction and circular economy. Industrial relevanceSpirulina plantensis represents a potential biomass feedstock due to its potential as a source of compounds of great economic value (including antioxidants, proteins, lipids and natural pigments, in particular blue colorants). The combined use of ohmic heating and enzymes in the aqueous extraction step fosters the use of environmentally friendly technologies to implement sequential high yield and high purity extraction of the different valuable fractions with bio-functional properties, targeting waste reduction and contributing to the implementation of circular economy strategies. This can be integrated with a design of Industry 4.0 driving the development of new products.

Extraction of Chlorophylls and Carotenoids from Microalgae: COSMO‐SAC‐Assisted Solvent Screening

AbstractEfficient pigment extraction is a key factor influencing the economic feasibility of microalgal biorefineries. Ethanol, acetone, ethyl acetate, and hexane were evaluated for the batch extraction of chlorophyll a, chlorophyll b, and carotenoids from Chlorella sorokiniana and Heterochlorella luteoviridis. Solvent‐to‐biomass ratios did not affect extraction results in most cases. Ethanol showed the highest efficiency in the recovery of chlorophylls, presenting high extraction yields. Acetone was among the most efficient solvents to extract violaxanthin, lutein, zeaxanthin, and carotene from both microalgae, proving to be generally suitable to this application. Conductor‐like screening model for real solvent segment activity (COSMO‐SAC) corroborated experimental results, proving to be an important tool in the screening of solvents for extraction processes.

The Present and Future of Less-invasive Liquid Biopsy for the Diagnosis of Gliomas and Brain Tumors

There is growing interest in liquid biopsy, the less-invasive detection of circulating tumor DNA(ctDNA)or circulating tumor cells(CTCs)from cerebrospinal fluid(CSF)and/or serum of patients, for the diagnosis of brain tumors. We share our experience of detecting hot spot point mutations using droplet digital PCR(ddPCR)in ctDNA obtained from the CSF of patients with brain tumors. The detection of mutations such as IDH1 R132H, BRAF V600E, and TERT promoter mutations in gliomas can be diagnostic. For optimal detection of ctDNA, which is only seen at very low concentrations, proper handling and storage of CSF, high-yield extraction of ctDNA, and usage of sensitive PCR methods for detection are imperative. We discuss which mutations can be assessed when diagnosing brain tumors, with a specific focus on gliomas. Finally, we look at what the near future holds for liquid biopsy of brain tumor patients, including next-generation sequencing panel analysis and accurate assessment of fusion genes.

Solid-Phase Microextraction of Phthalate Esters from Aqueous Media by Functionalized Carbon Nanotubes (Graphene Oxide Nanoribbons) and Determination by GC–FID

Multiwalled carbon nanotubes were exposed to hydrothermal treatment for obtaining graphene oxide nanoribbons (GONRs). The fabricated graphene oxide nanoribbons have been morphologically and compositionally characterized via FE-SEM, XRD, and FT-IR techniques. The as-synthesized GONRs have been used as sorbent phase for headspace solid-phase microextraction of phthalate esters (PEs) from aqueous solutions. In this regard, the GC–FID analysis route has been used for quantification of PEs. The new SPME fiber shows remarkable analytical figures of merit including broad dynamic linear ranges, low limits of detection, as well as good stability and reasonable relative standard deviations for evaluation of PEs. The linearity of the method for analysis of PEs including DnBP, DnPP, DEHP, DEHA, BBP, and DMP was between the range of 0.05–100, 0.05–100, 0.1–100, 0.1–100, 0.2–100, and 0.5–100 μg L−1, respectively. The limits of detection (based on S/N = 3) and correlation coefficients were found to be in the range of 0.02–0.2 μg L−1 and 0.9907–0.9952, correspondingly. The prepared GONR-coated SPME fiber shows larger extraction yield in comparison to pristine MWNTs and commercial PDMS SPME fibers. Furthermore, real sample analysis showed that there is no significant matrix effect for evaluation of PEs from environmental water samples and proposed method could be used for evaluation and determination of PEs from aqueous samples in a precise and accurate manner. The existence of functional groups, π–π interactions, as well as hydrogen bonding between adsorbent phase and PE analytes could be the reason for observing such a high extraction yield.

EVALUATION OF THE USE OF DIFFERENT SOLVENTS FOR PHYTOCHEMICAL CONSTITUENTS AND ANTIOXIDANTS ACTIVITY OF THE LEAVES OF MURRAYA KOENIGII (LINN.) SPRENG. (RUTACEAE)

Murraya koenigii (Rutaceae) is a promising source of bioactive compounds since the leaves of this plant has been traditionally used extensively in the Indian Ayurvedic system of medicine for the treatment of a wide range of diseases and disorders. Although the pharmacological effect of the plant’s bioactive compounds has been extensively studied, however, study on the effect of using different extraction solvents to extract these bioactive componentsis scarce. The aim of the present study was to evaluate the impact of different solvents on extraction yields, phytochemical constituents and antioxidants activity of dehydrated Murrayakoenigi leaves. The results showed that the used solvents play an important role in the yield of extraction and the content of chemical components. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (5.70%) as well as the highest content of phenolic (27.2 mg GAE/g DW) and flavonoid (15.55 mg QE/g DW). The extract obtained from methanol exhibited highest antioxidant scavenging activity (93%), (using 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging assay), and the antioxidant activity of Murraya koenigi leaves extract was found to be higher than ascorbic acid. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants constituents from Murraya koenigi leaves for utilization in pharmacognosy. To best of our knowledge this is the first report that directly compares these 4 extraction solvents for the extraction of bioactive components from Murraya koenigi leaves.

Optimization the conditions of process of production of pectin extracted from the waste of potato peel

The objective of this study is to investigate the effect of three variables including temperature (35, 65 and 95°C), time (40, 120 and 200) and pH (1, 2 and 3) on the yield, galacturonic acid percentage and degree of pectin esterification extracted from the potato peels and optimization of the condition of extraction. Response surface method is used to optimize the conditions of extraction. Physicochemical properties of extracted pectin under optimum conditions were compared with commercial citrus and apple pectins by pectin flow behavior tests at different concentrations, FT-IR spectrum and pectin molecular weight. The results showed that the highest extraction yield was 14.87% at 95°C, 120 min time and pH 1. The highest percentage of extracted galacturonic acid pectin in potato peel was 36.37% at 95°C in 120 min and pH 1. The highest degree of esterification of extracted pectin from potato peel was 41.820% at 65°C in 40 min time and pH 3.00. Simultaneous optimization of extracting pectin with the aim of achieving maximum yield was galacturonic acid with 100% desirability at 95°C, 200 min time and pH 1 that in this condition, the yield was 15.23% and galacturonic acid was 38.0712%. The highest stability of extracted pectin emulsion from potato peel was observed at 4°C on the first day. Also, the FT-IR results showed that the strong absorption between 3200-3500 cm-1 in the extracted pectin sample was related to the intracellular and extracellular vibration of the hydrogen bond in the galacturonic acid polymer. The molecular weight of extracted pectin from potato peel under optimum conditions was 53.46 kDa after 30 days of storage at 4 and 23°C with emulsion stability 85.1 and 63.1, respectively. The results of this research showed that extracted pectin from potato peel can be introduced as a source of pectin to the market.

Implementation in lipid extraction and analysis from phytoplankton: Skeletonema marinoi as case study

Microalgae, dominating protists in aquatic systems, are rich in lipids, a complex molecular class regulating cell physiology and phytoplankton ecology. Investigation of microalgal lipid metabolism is increasing, but common strategies for comprehensive lipid analyses are still lacking. Major methodological gaps are represented by separate analytical procedures for extraction and characterization of lipid molecules, thus hindering accurate comparison of metabolic data. Herein we propose one unique methodology for sample collection, extraction and analysis of main lipid mediators (oxylipins, fatty acids, glycerolipids, sterols and sterol derivatives) from diatoms. Extractions relied on the MTBE/methanol method, which provides high lipid extraction yields, involving either MeOH or water preparation of samples. Experiments focused on Skeletonema marinoi, a diatom species often used as model organism in plankton chemical ecology. Extraction protocols were implemented to characterize oxylipins and glycerolipids from algal cells collected as pellets or accumulated on glass-fibre filters, thus providing a practical tool to study multiple lipid classes in natural phytoplankton.

Yield and properties of nanobiosilica extracted from rice husk using technical grade chemicals: effect of extraction temperatures and times

Silica particles contained in rice husk (RH) are valuable materials with various industrial applications. However, simple and feasible techniques for large-scale production of silica nanoparticles from RH (nanobiosilica) remain challenging. This study was aimed to investigate the influence of extraction temperatures and times on the yield and characteristics of nanobiosilica extracted from rice husk ash (RHA) using technical grade chemicals. The nanobiosilica was extracted from RHA using a simple sol-gel technique at three temperatures (25, 50, and 70 ˚C) for three extraction times (1, 2, and 3 h). The results showed that the extraction yield of nanobiosilica was significantly influenced by the extraction temperatures and times. The highest extraction yield (67.2%) was obtained at a temperature of 70 ˚C for 3 h. The use of technical grade chemicals affected the quality of nanobiosilica produced. The concentration of SiO2 in nanobiosilica from the best treatment was 86.21%. The presence of asymmetric Si-O-Si functional group was observed at 1101.68 cm−1 wavelength. The nanobiosilica produced had particle sizes of 25.1-40.6 nm, bulk density of 0.67-0.81 g/mL, and averaged crystallinity degree of 58.50%. The best extraction technique developed may be used for large-production of nanobiosilica from RHA, depending on technical specifications required for further applications.

Synthesis of silica from rice husk by sol-gel method

Rice husk is an abundant and underutilized agricultural by-product in Indonesia. The silica contained in the waste could be used as a substitute natural silica for various purposes. The extraction of silica from rice husk (RH) has been done using the sol-gel method without involving high energy. Extraction was carried out with KOH at concentrations of 7, 10, and 13% for 90, 120, and 150 minutes. Experimental results showed that the highest extraction yield was obtained at the extraction process using KOH 7% (average 9.03%). The minimum concentration of KOH could produce the maximum yield of silica. Characterization by XRD showed that the silica had the same pattern as that of commercial products and it was categorized as amorphous silica indicated with the broad peaks at 2θ = 22°. Amorphous silica with high purity was produced from rice husk by KOH 7 % at 120 min (95.34 %). The process condition resulted in high reactivity and a high BET surface area. The washing process needs to be intensified to improve the purity of silica.

Interaction of herbal ingredients contained in the Trikatuk recipe: design of experiment (DOE) and chemical analysis point of view

Trikatuk is an herbal recipe used in Thai traditional medicines since ancient times. However, there is no scientific support about the use of the Trikatuk with an equal weight ratio of its herbal mixture. This work sought to apply the design of experiment to determine the interaction between Zingiber officinale Roscoe rhizomes, Piper nigrum L. fruits, and Piper retrofractum Vahl. infructescences, which are found in the traditional Trikatuk recipe. The chemical markers 6-gingerol and piperine were analyzed using high-performance liquid chromatography. A response surface analysis was also conducted to describe the level of interaction among the three herbal plants. The analysis method was a linear response, specific, precise, and accurate. Estimation of interaction generated using computer software were both precise and reliable. The high extraction yield was achieved at a high Piper retrofractum ratio. The high 6-gingerol content was found at the high Zingiber officinale ratio. The high piperine content was found at the equal ratio of Piper nigrum and Piper retrofractum. According to the interaction, the traditional Trikatuk recipe exhibited at least 200% increase in active compounds yield as compared with the effects of any of the plants individually. Data from this work could be used to describe the interaction of Trikatuk extract. Moreover, they may support that the use of the Trikatuk with an equal weight ratio distribution of the three herbal plants is in fact already appropriate.

The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

Bioactive Compounds and Evaluation of Antioxidant, Cytotoxic and Cytoprotective Effects of Murici Pulp Extracts (Byrsonima crassifolia) Obtained by Supercritical Extraction in HepG2 Cells Treated with H2O2.

The use of clean technologies in the development of bioactive plant extracts has been encouraged, but it is necessary to verify the cytotoxicity and cytoprotection for food and pharmaceutical applications. Therefore, the objective of this work was to obtain the experimental data of the supercritical sequential extraction of murici pulp, to determine the main bioactive compounds obtained and to evaluate the possible cytotoxicity and cytoprotection of the extracts in models of HepG2 cells treated with H2O2. The murici pulp was subjected to sequential extraction with supercritical CO2 and CO2+ethanol, at 343.15 K, and 22, 32, and 49 MPa. Higher extraction yields were obtained at 49 MPa. The oil presented lutein (224.77 µg/g), oleic, palmitic, and linoleic, as the main fatty acids, and POLi (17.63%), POO (15.84%), PPO (13.63%), and LiOO (10.26%), as the main triglycerides. The ethanolic extract presented lutein (242.16 µg/g), phenolic compounds (20.63 mg GAE/g), and flavonoids (0.65 mg QE/g). The ethanolic extract showed greater antioxidant activity (122.61 and 17.14 µmol TE/g) than oil (43.48 and 6.04 µmol TE/g). Both extracts did not show cytotoxicity and only murici oil showed a cytoprotective effect. Despite this, the results qualify both extracts for food/pharmaceutical applications.

Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction

The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method—hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP). The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined. UP, EP, and UEP had higher fucose and galactose compositions than HP. The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose. 1H NMR indicated that alginate was removed by purification. UP, EP, and UEP possessed higher sulfate contents than HP. UEP presented with the highest extraction yield and lowest protein and uronic acid contents. The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system. UEP and UP effectively inhibited AGE, although the inhibition effect was lower than that of aminoguanidine. However, UP and UEP showed higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl than aminoguanidine. AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight. Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.

A Novel Microfluidic Device for Blood Plasma Filtration.

The use of whole blood and some biological specimens, such as urine, saliva, and seminal fluid are limited in clinical laboratory analysis due to the interference of proteins with other small molecules in the matrix and blood cells with optical detection methods. Previously, we developed a microfluidic device featuring an electrokinetic size and mobility trap (SMT) for on-chip extract, concentrate, and separate small molecules from a biological sample like whole blood. The device was used to on-chip filtrate the whole blood from the blood cells and plasma proteins and then on-chip extract and separate the aminoglycoside antibiotic drugs within 3 min. Herein, a novel microfluidic device featuring a nano-junction similar to those reported in the previous work formed by dielectric breakdown was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield of 62% within less than 5 min. The filtered plasma was analyzed using our previous device to show the ability of this new device to remove blood cells and plasma proteins. The filtration device shows a high yield of plasma allowing it to detect a low concentration of analytes from the whole blood.

Obtaining extracts from Elaeagnus latifolia pulp using different environmentally friendly methods: Extraction kinetics, phenolic compounds content, and antioxidant activity

ABSTRACT Elaeagnus latifolia is an underutilized plant of the Elaeagnaceae family. The extraction of E. latifolia pulp was carried out from 30 to 120 min by different environmentally friendly extraction methods: agitation, percolation, Soxhlet, and ultrasound-assisted extraction with ethanol, and hydrodistillation. The extracts were analyzed for their total phenolic compounds, carotenoids and ascorbic acid contents, and antioxidant activity. The highest yield of extract, of 63.5 g/100 g of raw material (RM), in dry basis (db), total phenolic compounds, of 3.3 mg gallic acid equivalent (GAE)/g extract, and 2.042 mg GAE/g RM, and carotenoids, of 176.6 mg β-carotene equivalent/kg RM, were obtained by Soxhlet for 120 min. All the extracts presented similar antioxidant activity. Therefore, E. latifolia can be a source of compounds of interest to the food industry.

Long-chain alkanol–alkyl carboxylic acid-based low-viscosity hydrophobic deep eutectic solvents for one-pot extraction of anthraquinones from Rhei Radix et Rhizoma

Natural long-chain alkanol and alkyl carboxylic acid were used to prepare novel hydrophobic deep eutectic solvents (HDESs). These HDESs are liquid at room temperature and have low viscosity (<12.26 mPa‧s), low polarity (lower than that of methanol, ChCl-based deep eutectic solvents and other reported HDESs), and low density (<0.928 g/mL). A simple one-pot method based on a novel HDES–water two-phase extraction system was constructed for the extraction of weak-polarity bioactive components, anthraquinones, from Rhei Radix et Rhizoma. This HDES-based new extraction method does not consume hazardous organic solvents and can obtain a total anthraquinone yield of 21.52 mg/g, which is close to that obtained by the Chinese pharmacopoeia method (21.22 mg/g) and considerably higher than those by other reported HDESs-based extraction methods (14.20–20.09 mg/g, p < 0.01). The high extraction yield can be mainly attributed to the severe destruction of the RRR cell walls by the extraction system and the excellent dissolving ability of novel HDESs for anthraquinones.