High Expression Of Hsa Research Articles

RETRACTED ARTICLE: Circular RNA hsa_circ_0055538 regulates the malignant biological behavior of oral squamous cell carcinoma through the p53/Bcl-2/caspase signaling pathway

BackgroundOral squamous cell carcinoma (OSCC) is a common oral and maxillofacial malignant tumor with high rates of metastasis and mortality. Circular RNAs (circRNAs), a type of non-coding RNA, are involved in the development of a variety of tumors. The roles of circRNAs in OSCC are unclear; in this study, the correlation between the circRNA hsa_circ_0055538, previously identified by high-throughput sequencing, and the biological behavior of OSCC was evaluated.MethodscircRNA expression was evaluated using patient tissue samples and various OSCC cell lines. The effects of overexpression and knockdown were evaluated by lentiviral infection and siRNA transfection of the SCC9 and CAL27 cell lines. Migration, invasion, apoptosis, and the expression of proteins in the p53 signaling pathway were evaluated. Infected cells were injected into nude mice to evaluate tumorigenesis.ResultsLow hsa_circ_0055538 expression levels were verified in tumor tissues and OSCC cell lines. Clinical data analysis showed that the expression level is related to the degree of tumor differentiation. Lentiviral infection and siRNA transfection of SCC9 and CAL27 cell lines revealed that changes in circRNA expression significantly affected the malignant biological behavior of OSCC cells. Importantly, nude mouse experiments showed that high expression of hsa_circ_0055538 inhibited tumor growth. Finally, hsa_circ_0055538 may affect the development of OSCC via the p53/Bcl-2/caspase signaling pathway.ConclusionsOur results indicated that hsa_circ_0055538 is involved in OSCC via the p53 signaling pathway and may be a diagnostic and/or prognostic marker as well as a therapeutic target.

Abstract LB-391: Hsa_circ_001783 regulates breast cancer progression via sponging miR-200c

Abstract Introduction: Increasing evidence suggests circRNAs exert vital functions in tumor progression via sponging miRNAs. However, the role of circular RNAs in breast cancer remains mostly unclear. Here we reported the molecular mechanisms of a novel circRNA, hsa_circ_001783 in regulating breast cancer progression and its ability in predicting clinical outcomes by integrating high throughput computation, experimental technologies in vitro and clinical investigation. Methods: We extracted sequences and annotations of circRNAs and miRNAs from circbase and miRbase respectively. Four algorithms were used to predict the potential bindings of miRNAs to the conserved sequences of individual circRNAs. Computationally screening Ingenuity knowledge database (Qiagen), PubMed and Embase identified breast cancer associated miRNAs and their related functions in cancer. Five essential functional features were used to score the strength associations between miRNAs and breast cancer, which was subsequently employed to rank the network branches across circRNA-miRNA-breast cancer. Expression levels of hsa_circ_001783 in human breast tissue and cancer cells were quantified by FISH and qPCR. The effects of knocking down hsa_circ_001783 on breast cancer cells were examined by Edu, CCK-8, colony formation and transwell assays. K-M model was used to define the predictor performance of hsa_circ_001783 in a Chinese breast cancer cohort of 128 patients. Results: Our computational pipeline identified hsa_circ_001783 as the one with highest score out of 594 breast cancer-associated circRNA candidates. We found the circRNA was enriched in cytoplasma and overexpressed in breast tumor as compared to paired non-cancerous tissue. High expression of hsa_circ_001783 correlated with higher tumor burden (p=0.047) and poor overall survival (p=0.025) in 128 patients. Knock-down of hsa_circ_001783 remarkably inhibited the proliferation and invasion of MDA-MB-231 and MDA-MB-468 cells. We found hsa_circ_001783 increased significantly by 1.5-2 folds while 7 miRNAs, predicted targets of hsa_circ_001783, were remarkably reduced (fold change>1.5) in mRNA expression levels in BT549, MDA-MB-468 and MDA-MB-231 as compared with MCF-7 breast cancer cell lines. Among all the targets, miR-200c was the one in the strongest correlation with hsa_circ_001783 in expression levels. Knockdown of hsa_circ_00178 in MDA-MB-231 breast cancer cells suppressed expression of miR-200c-targeted genes ZEB1, ZEB2 and CCNA2. The expression level of hsa_circ_001783 in human breast cancer tissues negatively correlated with expression of miR-200c (p=0.0286), but positively correlated with that of ZEB1 (p=0.002), ZEB2 (p=0.0001) and CCNA2 (p=0.005). Conclusion: Hsa_circ_001783 regulates breast cancer progression via sponging miR-200c. The circRNA may serve as a novel predictor of clinical outcomes for breast cancer. Citation Format: Zihao Liu#, You Zhou#, Gehao Liang, Yun Ling, Luyuan Tan, Yan Wang, Wenjing Zhong, Chang Gong*. Hsa_circ_001783 regulates breast cancer progression via sponging miR-200c [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr LB-391.

Circular RNA hsa_circ_0021001 in peripheral blood: a potential novel biomarker in the screening of intracranial aneurysm.

Circular RNAs (circRNAs) in the peripheral blood have been reported to be associated with cancer. However, there are few studies about circRNAs in intracranial aneurysms (IA). The purpose of the current study was to investigate the characteristic expression of circular RNA hsa_circ_0021001 in the peripheral blood of patients with intracranial aneurysms and its potential as a diagnostic biomarker for IA. In this study, a cohort of 223 cases of IA patients who were admitted in the department of neurosurgery in the First People’s Hospital of Wenling from January 2009 to July 2012 were collected as the experimental group, and 131 healthy volunteers over the same period served as the control group. Peripheral blood of each subject in both groups was collected on an empty stomach. The expression of hsa_circ_0021001 in peripheral blood was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the difference was analyzed by paired t-test. The effectiveness of hsa_circ_0021001 in the diagnosis of IA was assessed by ROC curve. Multivariate Cox proportional hazards regression analysis was used to analyze the prognosis. Hsa_circ_0021001 level in the peripheral blood of IA patients was relatively lower than that in the control group (P=0.002). The area under ROC (AUC) was 0.87, indicating that hsa_circ_0021001 was highly effective in the diagnosis of IA. In addition, hsa_circ_0021001 expression was correlated with aneurysm rupture, Hunt, Hess level, and timing of surgery (P= 0.041, 0.013, and 0.001, respectively). Patients with high expression of hsa_circ_0021001 had longer disease-free survival (DFS) and overall survival (OS) (P < 0.05). We found for the first time that hsa_circ_0021001 decreased significantly in the peripheral blood of IA patients, which suggested that hsa_circ_0021001 might be used as a potential novel marker for the diagnosis of IA.

Comprehensive profile of differentially expressed circular RNAs reveals that hsa_circ_0000069 is upregulated and promotes cell proliferation, migration, and invasion in colorectal cancer.

BackgroundNowadays, despite great progress in cancer research, the detailed mechanisms of colorectal cancer (CRC) are still poorly understood. Circular RNAs (circRNAs), a new star of the non-coding RNA network, have been identified as critical regulators in various cancers, including CRC.Methods and resultsIn this study, by using unsupervised hierarchical clustering analysis, a novel dysregulated circRNA, hsa_circ_0000069, was found. The expression of hsa_circ_0000069 was measured in 30 paired CRC tissues and adjacent noncancerous tissues using quantitative polymerase chain reaction. A high expression of hsa_circ_0000069 was observed in CRC tissues and correlated with patients’ age and tumor, node, metastasis (TNM) stage (P<0.05). Furthermore, by using specifically designed siRNAs in CRC cells, a functional analysis was performed which revealed that hsa_circ_0000069 knockdown could notably inhibit cell proliferation, migration, and invasion, and induce G0/G1 phase arrest of cell cycle in vitro.ConclusionThis study’s findings are the first to demonstrate that hsa_circ_0000069, an important regulator in cancer progression, could be a promising target in the diagnosis and therapy in colorectal cancer.