High-elevation Mountain Research Articles

Impact of Climate Change on Vegetation Growth in Arid Northwest of China from 1982 to 2011

Previous studies have concluded that the increase in vegetation in the arid northwest of China is related to precipitation rather than temperature. However, these studies neglected the effects of climate warming on water availability that arise through changes in the melting characteristics of this snowy and glaciated region. Here, we characterized vegetation changes using the newly improved third-generation Global Inventory Modeling and Mapping Studies Normalized Difference Vegetation Index (GIMMS-3g NDVI) from 1982 to 2011. We analyzed the temperature and precipitation trends based on data from 51 meteorological stations across Northwest China and investigated changes in the glaciers using Gravity Recovery and Climate Experiment (GRACE) data. Our results indicated an increasing trend in vegetation greenness in Northwest China, and this increasing trend was mostly associated with increasing winter precipitation and summer temperature. We found that the mean annual temperature increased at a rate of 0.04 °C per year over the past 30 years, which induced rapid glacial melting. The total water storage measured by GRACE decreased by up to 8 mm yr−1 and primarily corresponded to the disappearance of glaciers. Considering the absence of any observed increase in precipitation in the growing season, the vegetation growth may have benefited from the melting of glaciers in high-elevation mountains (i.e., the Tianshan Mountains). Multiple regression analysis showed that temperature was positively correlated with NDVI and that gravity was negatively correlated with NDVI; together, these variables explained 84% of the NDVI variation. Our findings suggest that both winter precipitation and warming-induced glacial melting increased water availability to the arid vegetation in this region, resulting in enhanced greenness.

Will climate change increase the risk of plant invasions into mountains?

Mountain ecosystems have been less adversely affected by invasions of non-native plants than most other ecosystems, partially because most invasive plants in the lowlands are limited by climate and cannot grow under harsher high-elevation conditions. However, with ongoing climate change, invasive species may rapidly move upwards and threaten mid-, and then high-elevation mountain ecosystems. We evaluated this threat by modeling the current and future habitat suitability for 48 invasive plant species in Switzerland and New South Wales, Australia. Both regions had contrasting climate interactions with elevation, resulting in possible different responses of species distributions to climate change. Using a species distribution modeling approach that combines data from two spatial scales, we built high-resolution species distribution models (≤ 250 m) that account for the global climatic niche of species and also finer variables depicting local climate and disturbances. We found that different environmental drivers limit the elevation range of invasive species in each of the two regions, leading to region-specific species responses to climate change. The optimal suitability for plant invaders is predicted to markedly shift from the lowland to the montane or subalpine zone in Switzerland, whereas the upward shift is far less pronounced in New South Wales where montane and subalpine elevations are already suitable. The results suggest that species most likely to invade high elevations in Switzerland will be cold-tolerant, whereas species with an affinity to moist soils are most likely to invade higher elevations in Australia. Other plant traits were only marginally associated with elevation limits. These results demonstrate that a more systematic consideration of future distributions of invasive species is required in conservation plans of not yet invaded mountainous ecosystems.

Gas-particle partitioning and precipitation scavenging of polycyclic aromatic hydrocarbons (PAHs) in the free troposphere in southern China

Abstract Multi-phase (gaseous, particulate and rain) samples were collected simultaneously for the first time at a high-elevation mountain site in China during March to May 2009. The site, located in the free troposphere, is used to investigate the gas-particle partitioning, precipitation scavenging of polycyclic aromatic hydrocarbons (PAHs). The total measured concentrations of 15 USEPA PAHs varied from 2.67 to 137.00 ng/m 3 (average of 24.10 ng/m 3 ). Partitioning of PAHs between gaseous and particulate phases (log Kp) was well-correlated with the supercooled liquid vapor pressure in all samples (R 2 = 0.730–0.985), but the slopes (−0.154 to −0.424) were significantly deviated from the expected value of −1. The observed Kp values were better predicted by the Dual model than the Koa model, pointing to the importance of soot carbon for PAH partitioning. The scavenging ratios varied among the individual PAH compound, ranged from 4.47 × 10 3 (BaA) to 1.02 × 10 5 (ACY). Particle scavenging was the dominant removal process, accounting over 75% of the total scavenging (except for BbF) for individual PAH compound. Relationships of W p -particle fraction, W p -supercooled vapor pressure were analyzed. The results suggested the particle scavenging by precipitation is less efficient for PAHs with higher molecular weights, while more volatile PAHs with lower molecular weights deposited on larger atmospheric particles were scavenged more efficiently. The efficiency of W p was found to be increased with rain intensity. For gas scavenging, the dissolution and adsorption mechanisms shift their relative importance with different supercooled vapor pressure.

Recent temperature trends in the South Central Andes reconstructed from sedimentary chrysophyte stomatocysts in Laguna Escondida (1742 m a.s.l., 38°28 S, Chile)

In this study we present a quantitative, high resolution reconstruction of past austral winter length in the Chilean Andes at 38°S from AD 1920 to 2009. For Laguna Escondida, a nearly pristine lake situated on the flanks of the Andes at 1740m above sea level, past variability in the duration of the winter season (Days T4°C) was reconstructed. Because high elevation meteorological stations are absent in this region, the reconstruction provides novel insights into recent temperature trends in the central–southern Andes. As a cold-season temperature proxy, we used chrysophyte stomatocysts. This novel proxy for cold season temperature was so far applied successfully in the European Alps and Pyrenees but has not yet been tested in the Southern Hemisphere. The reconstruction in this study was based on a newly developed Transfer Function to estimate Days T4°C (number of consecutive days with surface water temperatures at or below 4°C) from sedimentary stomatocyst assemblages (R2boot=0.8, RMSEPboot=28.7days (=half the standard deviation)). To develop a high quality TF model, sediment traps and thermistors were placed in thirty remote lakes along an altitude gradient (420–2040ma.s.l.). Complete materials and data were collected in 24 lakes after one year. Detailed statistical analyses indicate that modern stomatocysts primarily respond to the length of the cold season. The TF model was then applied to the sedimentary stomatocysts from a 210Pb-dated short core of L. Escondida. Comparison to independent reanalysis data showed that reconstructed changes in Days T4°C provides detailed information on winter–spring temperature variability since AD 1920.The reconstruction shows that recent warming (onset in AD 1980) in the southern Chilean Andes was not exceptional in the context of the past century. This is in strong contrast to studies from the Northern Hemisphere. The finding is also in contrast to the cooling temperature trends which were detected using meteorological measurement data in low altitude sites along the Chilean coast. This finding confirms that coastal meteorological station data in this region do not reliably reflect recent temperature trends at high altitudes. Moreover, it implies a southward shift of the northern border of the Westerlies wind belt. This study clearly illustrates the importance of quantitative, high resolution studies from remote sites, in particular at high elevation mountain areas.

Successive reactivation of older structures under variable heat flow conditions evidenced by K–Ar fault gouge dating in Sierra de Ambato, northern Argentine broken foreland

The Argentine broken foreland has been the subject of continuous research to determine the uplift and exhumation history of the region. High-elevation mountains are the result of N–S reverse faults that disrupted a W–E Miocene Andean foreland basin. In the Sierra de Ambato (northern Argentine broken foreland) the reverse faults offset Neogene sedimentary rocks (Aconquija Fm., ∼9 Ma) and affect the basement comprising Paleozoic metamorphic rocks that have been dated at ∼477−470 Ma. In order to establish a chronology of these faults affecting the previous continuous basin we date the formation age of clay minerals associated with fault gouge using the K–Ar dating technique. Clay mineral formation is a fundamental process in the evolution of faults under the brittle regime (<<300 °C). K–Ar ages (9 fractions from 3 samples collected along a transect in the Sierra de Ambato) vary from Late Devonian to Late Triassic (∼360–220 Ma). This age distribution can be explained by a long lasting brittle deformation history with a minimum age of ∼360 Ma and a last clay minerals forming event at ∼220 Ma. Moreover, given the progression of apparent ages decreasing from coarse to fine size fractions (∼360−311 Ma for 2−1 μm grain size fraction, ∼326−286 Ma for 1–0.2 μm and ∼291−219 Ma of <0.2 μm), we modeled discrete deformation events at ∼417 Ma (ending of the Famatinian cycle), ∼317–326 Ma (end of Gondwanic orogeny), and ∼194–279 Ma (Early Permian - Jurassic deformation). According to our data, the Neogene reactivation would not have affected the K–Ar system neither generated a significant clay minerals crystallization in the fault gouge, although an exhumation of more than 2 Km is recorded in this period from stratigraphic data.

Organic and inorganic components of aerosols over the central Himalayas: winter and summer variations in stable carbon and nitrogen isotopic composition.

The aerosol samples were collected from a high elevation mountain site, Nainital, in India (1958 m asl) during September 2006 to June 2007 and were analyzed for water-soluble inorganic species, total carbon, nitrogen, and their isotopic composition (δ(13)C and δ(15)N, respectively). The chemical and isotopic composition of aerosols revealed significant anthropogenic influence over this remote free-troposphere site. The amount of total carbon and nitrogen and their isotopic composition suggest a considerable contribution of biomass burning to the aerosols during winter. On the other hand, fossil fuel combustion sources are found to be dominant during summer. The carbon aerosol in winter is characterized by greater isotope ratios (av. -24.0‰), mostly originated from biomass burning of C4 plants. On the contrary, the aerosols in summer showed smaller δ(13)C values (-26.0‰), indicating that they are originated from vascular plants (mostly of C3 plants). The secondary ions (i.e., SO4 (2-), NH4 (+), and NO3 (-)) were abundant due to the atmospheric reactions during long-range transport in both seasons. The water-soluble organic and inorganic compositions revealed that they are aged in winter but comparatively fresh in summer. This study validates that the pollutants generated from far distant sources could reach high altitudes over the Himalayan region under favorable meteorological conditions.

Spatiotemporal characteristics of cultural sites and their driving forces in the Ili River Valley during historical periods

This study applies ArcGIS to analyze the spatiotemporal distribution of cultural sites in the Ili River Valley in northwestern China. It explores relationships between the sites’ spatiotemporal evolutionary characteristics, human history, and the natural environment. The results indicate that the numbers and proportions of the sites, and the frequency of their occurrence, exhibited an inverted V-shaped change trend during six historical periods. The “high in the east and low in the west” spatial distribution pattern of the first three periods shifted to the one the “high in the west and low in the east” during the latter three periods, demonstrating a change in the spatial center of gravity of human activities. The sites were mainly distributed on slopes of grades 1–5, with their proportions increasing from 75% during the Spring and Autumn Period (770 BC–476 BC)–Qin Dynasty (221 BC–207 BC) to 93.75% during the Qing Dynasty–Modern period. The concentrated distribution of site elevations shifted from grades 4–8 during the Spring and Autumn Period–Qin Dynasty, and the Western Han (206 BC–8)–Southern and Northern Dynasties (420–589), to grades 1–4 during the latter three periods. The number of sites showed a shifting trend from high-elevation mountains and hills to low-elevation plains, and from high slopes to low slopes. In particular, the sites exhibited a special “moist” evolutionary pattern of migration from middle and upstream areas to downstream areas, as opposed to the migration pattern of sites located in typical arid areas. The study also considered factors influencing the distribution and spatiotemporal evolution of cultural sites, notably, human factors and natural factors.

The relative contributions of alpine and subalpine ecosystems to the water balance of a mountainous, headwater catchment

Climate change is affecting the hydrology of high-elevation mountain ecosystems, with implications for ecosystem functioning and water availability to downstream populations. We directly and continuously measured precipitation and evapotranspiration (ET) from both subalpine forest and alpine tundra portions of a single catchment, as well as discharge fluxes at the catchment outlet, to quantify the water balance of a mountainous, headwater catchment in Colorado, USA. Between 2008 and 2012, the water balance closure averaged 90% annually, and the catchment ET was the largest water output at 66% of precipitation. Alpine ET was greatest during the winter, in part because of sublimation from blowing snow, which contributed from 27% to 48% of the alpine, and 6% to 9% of the catchment water balance, respectively. The subalpine ET peaked in summer. Alpine areas generated the majority of the catchment discharge, despite covering only 31% of the catchment area. Although the average annual alpine runoff efficiency (discharge/precipitation; 40%) was greater than the subalpine runoff efficiency (19%), the subalpine runoff efficiency was more sensitive to changes in precipitation. Inter-annual analysis of the evaporative and dryness indices revealed persistent moisture limitations at the catchment scale, although the alpine alternated between energy-limited and water-limited states in wet and dry years. Each ecosystem generally over-generated discharge relative to that expected from a Budyko-type model. The alpine and catchment water yields were relatively unaffected by annual meteorological variability, but this interpretation was dependent on the method used to quantify potential ET. Our results indicate that correctly accounting for dissimilar hydrological cycling above and below alpine treeline is critical to quantify the water balance of high-elevation mountain catchments over periods of meteorological variability. Copyright © 2015 John Wiley & Sons, Ltd.

Characterization of carbonaceous aerosols at Mount Lu in South China: implication for secondary organic carbon formation and long-range transport.

In order to understand the sources and potential formation processes of atmospheric carbonaceous aerosols in South China, fine particle samples were collected at a high-elevation mountain site--Mount Lu (29°35' N, 115°59' E, 1165 m A.S.L.) during August-September, 2011. Eight carbonaceous fractions from particles were resolved following the IMPROVE thermal/optical reflectance protocol. During the observation campaign, the daily concentrations of PM2.5 at Mount Lu ranged from 7.69 to 116.39 μg/m(3), with an average of 58.76 μg/m(3). The observed average organic carbon (OC) and elemental carbon (EC) concentrations in PM2.5 were 3.78 and 1.28 μg/m(3), respectively. Secondary organic carbon (SOC) concentration, estimated by EC-tracer method, was 2.07 μg/m(3) on average, accounting for 45.0% of the total OC. The enhancement of secondary organic aerosol (SOA) formation was observed during cloud/fog processing, and heterogeneous acid-catalyzed reactions may have contributed to SOA formation as well. Back trajectory analysis indicated that air masses were mainly sourced from southern China during observation period, and this air mass source was featured by highest values of OC and effective carbon ratio (ECR). Relation of carbonaceous species and principal component analysis indicated that multiple sources contributed to the carbonaceous aerosols at Mount Lu.

Chemical composition and size distribution of summertime PM&amp;lt;sub&amp;gt;2.5&amp;lt;/sub&amp;gt; at a high altitude remote location in the northeast of the Qinghai–Xizang (Tibet) Plateau: insights into aerosol sources and processing in free troposphere

Abstract. Aerosol filter samples were collected at a high-elevation mountain observatory (4180 m a.s.l.) in the northeastern part of the Qinghai–Xizang (Tibet) Plateau (QXP) during summer 2012 using a low-volume sampler and a micro-orifice uniform deposit impactor (MOUDI). These samples were analyzed for water-soluble inorganic ions (WSIs), organic carbon (OC), elemental carbon (EC), water-soluble organic carbon (WSOC), and total organic nitrogen (TON) to elucidate the size-resolved chemical composition of free tropospheric aerosols in the QXP region. The average mass concentration of the sum of the analyzed species in PM2.5 (particle matter) (WSIs + OC + EC + TON) was 3.74 μg sm−3, 36% of which was sulfate, 18% OC, 17 % nitrate, 10% ammonium, 6.6% calcium, 6.4% TON, 2.6% EC, 1.5 % sodium, 0.9% chloride, 0.5% magnesium, and 0.3% potassium. The size distributions of sulfate and ammonium peaked in the accumulation mode (0.32–0.56 μm), whereas the size distributions of both nitrate and calcium peaked in the range of 1.8–3.2 μm, suggesting the formation of nitrate on mineral dust. OC, EC and TON were also predominantly found in the accumulation mode. The bulk chemical composition and the average oxidation degree of water-soluble organic matter (WSOM) were assessed using a high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS). WSOM was found to be highly oxidized in all PM2.5 samples with an average oxygen-to-carbon atomic ratio (O / C) of 1.16 and an organic mass-to-organic carbon ratio (OM / OC) of 2.75. The highly oxidized WSOM was likely related to active cloud processing during upslope air mass transport coupled with strongly oxidizing environments caused by snow/ice photochemistry. High average ratios of OC / EC (7.6) and WSOC / OC (0.79) suggested that organic aerosols were primarily made of secondary species. Secondary organic aerosol (SOA) was estimated on average accounting for 80% (62–96%) of the PM2.5, indicating that SOA is an important component of free tropospheric aerosols over the northern QXP.

Source limitation of carbon gas emissions in high-elevation mountain streams and lakes

Inland waters are an important component of the global carbon cycle through transport, storage, and direct emissions of CO2 and CH4 to the atmosphere. Despite predictions of high physical gas exchange rates due to turbulent flows and ubiquitous supersaturation of CO2—and perhaps also CH4—patterns of gas emissions are essentially undocumented for high mountain ecosystems. Much like other headwater networks around the globe, we found that high-elevation streams in Rocky Mountain National Park, USA, were supersaturated with CO2 during the growing season and were net sources to the atmosphere. CO2 concentrations in lakes, on the other hand, tended to be less than atmospheric equilibrium during the open water season. CO2 and CH4 emissions from the aquatic conduit were relatively small compared to many parts of the globe. Irrespective of the physical template for high gas exchange (high k), we found evidence of CO2 source limitation to mountain streams during the growing season, which limits overall CO2 emissions. Our results suggest a reduced importance of aquatic ecosystems for carbon cycling in high-elevation landscapes having limited soil development and high CO2 consumption via mineral weathering.

Bacterial community of cushion plant Thylacospermum ceaspitosum on elevational gradient in the Himalayan cold desert.

Although bacterial assemblages are important components of soils in arid ecosystems, the knowledge about composition, life-strategies, and environmental drivers is still fragmentary, especially in remote high-elevation mountains. We compared the quality and quantity of heterotrophic bacterial assemblages between the rhizosphere of the dominant cushion-forming plant Thylacospermum ceaspitosum and its surrounding bulk soil in two mountain ranges (East Karakoram: 4850–5250 m and Little Tibet: 5350–5850 m), in communities from cold steppes to the subnival zone in Ladakh, arid Trans-Himalaya, northwest India. Bacterial communities were characterized by molecular fingerprinting in combination with culture-dependent methods. The effects of environmental factors (elevation, mountain range, and soil physico-chemical parameters) on the bacterial community composition and structure were tested by multivariate redundancy analysis and conditional inference trees. Actinobacteria dominate the cultivable part of community and represent a major bacterial lineage of cold desert soils. The most abundant genera were Streptomyces, Arthrobacter, and Paenibacillus, representing both r- and K-strategists. The soil texture is the most important factor for the community structure and the total bacteria counts. Less abundant and diverse assemblages are found in East Karakoram with coarser soils derived from leucogranite bedrock, while more diverse assemblages in Little Tibet are associated with finer soils derived from easily weathering gneisses. Cushion rhizosphere is in general less diverse than bulk soil, and contains more r-strategists. K-strategists are more associated with the extremes of the gradient, with drought at lowest elevations (4850–5000 m) and frost at the highest elevations (5750–5850 m). The present study illuminates the composition of soil bacterial assemblages in relation to the cushion plant T. ceaspitosum in a xeric environment and brings important information about heterotrophic bacteria in Himalayan soil.

Vegetation change at high elevation: scale dependence and interactive effects on Niwot Ridge

Background: High-elevation mountain systems may be particularly responsive to climate change.Aims: Here we investigate how changes along elevation gradients in mountain systems can aid in predicting vegetation distributional changes in time, focusing on how changing climatic controls affect meso-scale transitions at the lower and upper boundaries of alpine vegetation (with forest and subnival zones, respectively) as well as micro-scale transitions among plant communities within the alpine belt. We focus on climate-related drivers, particularly in relation to climate change, but also consider how species interactions, dispersal and responses to disturbance may influence plant responses to these abiotic drivers.Results: Empirical observations and experimental studies indicate that changing climatic controls influence both meso-scale transitions at the upper and lower boundaries of alpine vegetation and micro-scale transitions among plant communities within tundra. Micro-scale heterogeneity appears to buffer response in many cases, while interactions between climate and other changes may often accelerate change.Conclusions: Interactions with microtopography and larger edaphic gradients have the capacity to both facilitate rapid changes and reinforce stability, and that these interactions will affect the responsiveness of vegetation to climate change at different spatial scales.

Asymmetric gene flow and the distribution of genetic diversity in morphologically distinct Abies mariesii populations in contrasting eco-habitats

The steep and complex topography in high-elevation mountains usually generates heterogeneous habitats, where reproductive isolation in spatially discrete populations, such as unsynchronized flowering, likely occurs. Therefore, genetically differentiated populations in some cases can be expected. Patterns of genetic variation and directions of gene flow among discrete populations could hold crucial information for a better understanding of population genetic structures in heterogeneous habitats. In this study, we examined the local-scale genetic structure and gene flow patterns among Abies mariesii populations in a heterogeneously snowy subalpine ecosystem. We found that A. mariesii populations, though spatially discrete and undergoing strong habitat heterogeneity, are less likely to be genetically differentiated. Gene flow was biased toward the moorlands, and compared to the forest populations, the genetic diversity in the moorland populations was found to be significantly higher, suggesting that the moorlands could serve as sinks of genetic diversity for A. mariesii. Finally, we quantified the morphological variation of A. mariesii and discovered that being able to adjust tree morphology in contrasting eco-habitats may strengthen the competitiveness of A. mariesii. This may also make a tangible contribution to the maintenance of populations in contrasting habitats.

Case Study: Long-Term Livestock Grazing Influence on Vegetation Class in Coyote Flat, California, USA

On the Ground Parker Three-Step data that exist on many US Forest Service allotments may be the only remaining, truly long-term vegetation and soil data available. Although Parker Three-Step procedures have been abandoned on many Forest Service districts, the historical insight they provide may be worth revisiting for management purposes. The Parker photos that accompany the transect data may be of more value than the data. Long-term vegetation records in Coyote Flat reveal the range to remain generally in fair condition since at least 1931, despite large reductions in livestock numbers, drastically shortened season of use, and 7 years of rest out of the last 13 grazing seasons. The correlation and interaction between reduced grazing pressure and ecological condition on high-elevation mountain meadow ecosystems, particularly as revealed by Parker Three-Step data, is not always intuitive or linear.

Genetic structure in Pinus cembra from the Carpathian Mountains inferred from nuclear and chloroplast microsatellites confirms post-glacial range contraction and identifies introduced individuals

Genetic differentiation of scattered populations at neutral loci is characterized by genetic drift counteracted by the remaining gene flow. Populations of Pinus cembra in the Carpathian Mountains are isolated and restricted to island-like stands at high-elevation mountain ranges. In contrast, paleobotanical data suggest an extended early Holocene distribution of P. cembra in the Carpathians and its surrounding areas, which has contracted to the currently disjunct occurrences. We analyzed the genetic variation of 11 Carpathian populations of P. cembra at chloroplast and, in part newly developed, nuclear microsatellites. Both marker types revealed low levels of genetic differentiation and a lack of isolation by distance, reflecting the post-glacial retraction of the species to its current distribution. Stronger effects of genetic drift were implied by the higher genetic differentiation found for haploid chloroplast than for diploid nuclear markers. Moreover, we found no association between the values of population genetic differentiation for the two marker types. Several populations indicated recent genetic bottlenecks and inbreeding as a consequence of decline in population sizes. Moreover, we found individuals in two populations from the Rodnei Mountains that strikingly differed in assignment probabilities from the remaining specimens, suggesting that they had been introduced from a provenance outside the studied populations. Comparison with Eastern Alpine P. cembra and individuals of the closely related Pinus sibirica suggests that these individuals presumably are P. sibirica. Our study highlights the importance of the maintenance of sufficiently large local population sizes for conservation due to low connectivity between local occurrences.

Mercury distribution and variation on a high-elevation mountain glacier on the northern boundary of the Tibetan Plateau

In July 2013, snow/ice and streamwater samples were retrieved from a high-elevation mountain glacier (Laohugou Glacier No. 12) on the northern boundary of the Tibetan Plateau and were analysed for total Hg (HgT). Concentration levels of HgT in snow samples were higher than those from the central and southern part of the Tibetan Plateau. Most of the Hg in the snow/ice was found to be associated with particulate matter. A small daily variation of HgT in surface snow further suggested that snow Hg on the Tibetan Plateau was less influenced by the effect of post-depositional processes such as photoreduction. The variations in HgT peak concentration on the vertical profiles of the snowpits indicated that settling of particulate matter and percolation of snowmelt had caused an increase in Hg concentrations with increasing depth. No positive relationship was found between HgT and elevation in our study region, indicating that the “altitude effect” on Hg in surface snow may only exist on extremely high-elevation glaciers. Moreover, HgT concentrations in the surface ice were found to be the highest observed in this glacier. With ongoing climate warming, this is of particular concern in western China as the accumulated Hg released by accelerated glacier thinning and retreat may endanger ecosystems and human health in the glacier-fed downstream regions.

Comparative biogeography of the congener lilies Lilium distichum and Lilium tsingtauense in Korea

The main Korean mountain range (the so-called “Baekdudaegan”), which stretches from north to south across most of the Peninsula, has been thought to harbor glacial refugia for boreal and temperate plant species, where they likely found relatively stable habitats and maintained large population sizes. Under this scenario, high levels of genetic variation for plant populations can be anticipated. To test this hypothesis, we examined levels of allozyme diversity in the boreal herb Lilium distichum, which in Korea occurs largely along the Baekdudaegan and in its closely related congener L. tsingtauense, a temperate species that can occur from the coastal islands to high-altitude mountains in the Baekdudaegan. As expected, L. distichum harbored high levels of genetic variation within populations, and we found significant correlations between percentage of polymorphic loci (and allelic richness) and elevation. For L. tsingtauense we found moderate genetic variation, with its populations showing a significantly positive correlation between intra-population expected heterozygosity and elevation. Our results suggest that populations/species that occur within or near the main ridge of the Baekdudaegan probably endured the glacial periods in macrorefugia, whereas those populations/species located at low elevations (far away from mid- and high-elevation mountains) did it in microrefugia. Palaeodistribution modelling is in agreement with genetic data, indicating that the Baekdudaegan was suitable habitat for both species during the glacial (LGM) and interglacial periods (like the present). This study as well as the data from other studies compiled here provides strong evidence that the Baekdudaegan was a major refugial area for the East Asian flora throughout the Quaternary and, thus, meriting high priority for conservation biogeography.

Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells.

BackgroundIn the past several decades, Polygonum viviparum L. (PV) was reported to have antibacterial, antiulcer, antioxidant, antitumor, anti-inflammatory, and antiarthritic properties. The anti-inflammatory pathway was recently elucidated through cytosolic nuclear factor E2-related factor 2 (Nrf2) activation and heme oxygenase (HO)-1 protein expression. PV is a perennial herb and widely distributed in high-elevation mountain regions, such as the Tibetan Plateau. In Tibetan traditional medicine, PV is usually used to boost the blood circulation to dissipate blood stasis. Therefore, this study focused on how PV improves the vascular circulation and acts on vascular tissues.MethodsIn this study, we isolated aortas from Sprague-Dawley rats (male, weight about 250 ~ 350 g), and detected the effects of PV on phenylephrine (PE)-induced contraction and cyclic guanosine 3′,5′-monophosphate (cGMP) formation using aortic rings. In addition, human umbilical vein endothelial cells (HUVECs) were used to exam nitric oxygen (NO) synthase (NOS) activity by directly measuring NO production in the culture medium. Endothelial (e) NOS phosphorylation, and cytosolic Nrf2 and HO-1 expressions were measured using a Western blot analysis.ResultsPV dose-dependently relaxed PE-induced contractions in endothelial-intact but not -denuded aorta. The concentration to produce 50% relaxation was 22.04 ± 1.77 μg/ml. PV-induced vasorelaxation was markedly blocked by pretreatment with NG-nitro-L-arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue (MB), a guanylyl cyclase inhibitor, and hemoglobin, an NO scavenger. PV increased cGMP formation; however, this effect was also suppressed by co-pretreatment with l-NAME, MB, hemoglobin, and Ca2+-free medium. In HUVECs, PV increased NO formation, which was greatly attenuated by NOS inhibitors (L-NAME and L-NMMA) and by removing extracellular Ca2+ and chelating intracellular Ca2+ with BAPTA-AM. In addition, PV promoted eNOS phosphorylation, Nrf2 degradation, and HO-1 protein expression according to a Western blot analysis.ConclusionsThe results suggest that PV possesses vasorelaxing action in an endothelium-dependent manner and works through activating Ca2+/calmodulin- dependent NO synthesis; when NO is released and then transferred to smooth muscle cells, NO activates guanylyl cyclase and increases cGMP formation, ultimately resulting in vasorelaxation. Thus, PV can be considered for application as a potential therapeutic approach for vascular-associated disorders.

Recent changes in freezing level heights in High Asia and their impact on glacier changes

The heights of the atmospheric freezing level have increased over most glacierized areas of High Asia during 1971-2010, especially in the Altai Mountains, the eastern Tianshan Mountains, and the northeastern margins of the Tibetan Plateau. The systematic increase of freezing level heights (FLHs) is evidenced from both radiosonde and National Centers for Environmental Prediction/National Center for Atmospheric Research reanalysis data. Eleven glaciers with long-term observations are selected in typical high-elevation mountain ranges to examine the relationship between changes in FLHs and cryospheric response. Long-term trends in glacier mass balance and equilibrium line altitude (ELA) show significant correlations with changes in FLHs. A rise of 10 m in summer FLH causes mass balance of reference glaciers in High Asia to decrease by between 7 and 38mm (water equivalent) and ELA to increase by between 3.1 and 9.8m, respectively, depending on location. Both relationships are statistically significant (p<0.01) for most reference glaciers. Thus, rapid deglaciation in these high mountain ranges during recent decades is related to the increase in FLH. Similar relationships may exist in other high-elevation glaciers of High Asia with changes in FLHs having significant ecological and social consequences, especially in arid and semiarid regions.