High Nitrogen Removal Efficiency Research Articles

Start-up of a combined anaerobic/partial nitritation/anammox process for high-salt mustard wastewater treatment.

To treat high salinity wastewater from the mustard pickling industry, a combined anaerobic, partial nitritation (PN), and anammox process was employed using three connected reactors: an anaerobic sequencing batch biofilm reactor (ASBBR) for anaerobic treatment, a sequencing batch reactor (SBR) for PN, and an upflow anaerobic sludge blanket (UASB) for anammox. The start-up of the three individual reactors was investigated. Results showed that each reactor started up successfully, notwithstanding the stepwise increase of influent salinity to about 16.1 g NaCl/L. In the ASBBR, 89.7 % of chemical oxygen demand in the influent was removed and organic nitrogen was converted to ammonium (NH4 (+)-N). The SBR performed well with NO3 (-)-N concentration of 4.9 mg/L and ratio of NO2 (-)-N to NH4 (+)-N at the range of 1.0 to 1.3 in the effluent, which favored the anammox process. After the start-up of the UASB, the anammox process also showed stability and efficiency with a high total nitrogen removal efficiency of 86.2 % under high salinity of 12.0 g NaCl/L and nitrogen loading rate of 258 mg/(L · day).

Effect of temperature change on anammox activity.

Autotrophic nitrogen removal appears as a prerequisite for the implementation of energy autarchic municipal wastewater treatment plants. Whilst the application of anammox-related technologies in the side-stream is at present state of the art, the feasibility of this energy-efficient process in main-stream conditions is still under investigation. Lower operating temperatures and ammonium concentrations, together with a demand for high and stable nitrogen removal efficiency, represent the main challenges to overcome for this appealing new frontier of the wastewater treatment field. In this study, we report the short-term effect of temperature on the maximum biomass specific activity of anaerobic ammonium oxidizing (anammox) bacteria as evaluated by means of batch tests. The experiments were performed on anammox biomass sampled from two full-scale reactors and two lab-scale reactors, all characterized by different reactor configurations and operating conditions. The results indicate that for the anammox conversion, the temperature dependency cannot be accurately modeled by one single Arrhenius coefficient (i.e., θ) as typically applied for other biological processes. The temperature effect is increasing at lower temperatures. Adaptation of anammox bacteria after long-term cultivation at 20 and 10°C was observed. Implications for modeling and process design are finally discussed.

Application of a partial nitritation and anammox system for the old landfill leachate treatment

This study shows high nitrogen removal efficiency of a combination of partial nitritation and Anaerobic Ammonium Oxidation (anammox) process for old landfill leachate treatment. A lab-scale experiment including partial nitritation using a sequencing batch reactor (PN-SBR) followed by a anammox hybrid reactor (HAR), which consists of suspended biomass layer in the bottom part and bio-carrier bed in the upper part run at the influent total ammonia concentrations (TAN) of 500 mg N/L and 1000 mg N/L. The result of PN-SBR experiment showed that the NO2–N:NH4–N ratio achieved about 1.22 and 1.02 at HRT of 12 h (influent TAN of 500 mg N/L) and HRT of 19 h (influent TAN of 1000 mg N/L), respectively. Simultaneously, the HAR was operated at the nitrogen loading rate (NLR) of 4.2 and 8.3 kg N/m3.d, corresponding to influent TAN of 500 mg N/L and 1000 mg N/L, respectively. The effluent of the system containing 9.7 ± 3.5 mg NH4–N/L, 1.7 ± 0.4 mg NO2–N/L and 23 ± 4 mg NO3–N/L (equivalent to 35 ± 4 mg TN/L) at NLR of 1.02 for PN-SBR and NLR of 4.2 kg N/m3.d for HAR. This effluent quality was good enough to meet Vietnamese treated landfill leachate quality. The system obtained TN removals of 93 ± 1% and 81 ± 1.2% at NLRs of 4.2 kg TN/m3.d (phase I) and 8.3 kg TN/m3.d (phase II), respectively. The total biomass of HAR including attached biomass and suspended one was maintained up to 20,400 mgVSS/L at the end of the experiment when the removal rate of anamnox biomass obtained 0.4 kg TN/kgVSS.d. While, PN-SBR was kept at 2300 mg MLVSS/L and SRT of 10–12 days at NLR of 4.2 kg TN/m3.d, which the nitrogen conversion rate of AOB was 0.53 kg TAN/kgVSS.d. In terms of COD removal, it is found that PN-SBR removed only 14% of influent COD, whereas HAR removed 30% of COD.

The effect of light supply on microalgal growth, CO2 uptake and nutrient removal from wastewater

Microalgal based biofuels have been reported as an attractive alternative for fossil fuels, since they constitute a renewable energy source that reduces greenhouse gas emissions to the atmosphere. However, producing biofuels from microalgae is still not economically viable. Therefore, the integration of biofuel production with other microalgal applications, such as CO2 capture and nutrient removal from wastewaters, would reduce the microalgal production costs (and the environmental impact of cultures), increasing the economic viability of the whole process. Additionally, producing biofuels from microalgae strongly depends on microalgal strain and culture conditions.This study evaluates the effect of culture conditions, namely light irradiance (36, 60, 120 and 180μEm−2s−1) and light:dark ratio (10:14, 14:10 and 24:0), on microalgal growth, atmospheric CO2 uptake and nutrient (nitrogen and phosphorous) removal from culture medium. Four different microalgal strains, Chlorella vulgaris, Pseudokirchneriella subcapitata, Synechocystis salina and Microcystis aeruginosa, were studied to ascertain the most advantageous regarding the referred applications.This study has shown that higher light irradiance values and light periods resulted in higher specific growth rates and CO2 uptake rates. C. vulgaris presented the highest specific growth rate and CO2 uptake rate: 1.190±0.041d−1 and 0.471±0.047gCO2L−1d−1, respectively. All the strains have shown high nitrogen removal efficiencies, reaching 100% removal percentages in cultures with higher light supply. Phosphorus removal increased with light irradiance and with light:dark ratio. The highest removal efficiency, 67.6±7.1%, was achieved by the microalga C. vulgaris.

A single-stage biological process for municipal sewage treatment in tourist areas

This pilot scale study aims to test the effectiveness of an innovative compact biological system (SBBGR – Sequencing Batch Biofilter Granular Reactor) for treating municipal wastewater in tourist areas characterised by intense seasonal water demand and wastewater discharge. The results obtained after a long term operation of 463 days have shown that the proposed system is able to assure average removal efficiencies higher than 90% for COD (chemical oxygen demand), total suspended solids and TKN (total Kjeldahl nitrogen) independently of the influent concentration values and organic loading, which ranged from 0.2 to 5.1 kgCOD/m3biofilter.d Furthermore, the plant showed a high degree of operation flexibility and stability in response to the organic load variations occurring in tourist areas. In fact, no significant deterioration in the plant's effluent quality was observed even during a sudden several-fold increase in organic loading. High nitrogen removal efficiencies (80%, on average) were also achieved thanks to the establishment of simultaneous nitrification-denitrification process favoured by the plant's high biomass concentration and operating conditions. Finally, the system was characterized by an excess sludge production much lower (60–80% lower) than that of conventional biological systems operating without a primary clarifier. An acceptable level of stabilization of excess sludge was also obtained so that a further stabilization process was no longer required.

Isolation and characterization of a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB for bioremediation of wastewater

A novel heterotrophic nitrifying and aerobic denitrifying bacterium, KTB, was isolated from activated sludge flocci collected from a biological aerated filter according to the modified Takaya method and identified as Pseudomonas stutzeri by 16S rDNA gene sequence analysis. When shaking-cultured in the presence of 4.331 mmol/L of nitrate, 4.511 mmol/L of nitrite and 4.438 mmol/L of ammonium, the strain grew fast, with μmax being 0.42, 0.45, and 0.56/h, and displayed high nitrogen removal efficiency, with nitrogen removal rate being 0.239, 0.362, and 0.361 mmol/L/h and nitrogen removal ratio being 99.1, 100.0, and 100.0% in 18 h, respectively. The removal mainly occurred in the logarithmic phase. Nitrite accumulation did not affect denitrification performance. Nitrate concentration was below the detectable limit during the whole growth cycle when ammonium was used as sole nitrogen source. It tolerated high DO level and exhibited excellent aggregation ability. A possible pathway involved in the nitrogen removal process, which demonstrated a full nitrification and denitrification route, was speculated. The strain might be a great candidate for biological removal of nitrogen compounds from wastewater.

ANAMMOX performance, granulation, and microbial response under COD disturbance

BACKGROUND ANAMMOX (ANaerobic AMMonium OXidation), a promising process for biological nitrogen removal, is usually affected by organic matter. In order to fully understand the effect of chemical oxygen demand (COD) on the ANAMMOX process, reactor performance, granular characteristics and microbial response were evaluated systematically under glucose stimulation. RESULTS High removal efficiencies of ammonium and total nitrogen (> 90%) were achieved with 100 mg L-1 COD concentration. ANAMMOX contribution was reduced to only 69% as COD concentration was increased to 300 mg L-1. The granulation, EPS contents, TB-EPS/LB-EPS and protein/carbohydrate were increased at a specific COD, with higher COD disrupting the biofacies structure. The results also showed that EPS distribution and microbial structure were affected by the organic disturbance. CONCLUSION An appropriate COD concentration enhanced the total nitrogen removal during ANAMMOX process, by enabling a stable synergism between ANAMMOX bacteria and heterotrophic denitrificans. Furthermore, a proper COD stimulation promoted sludge granulation by influencing EPS composition and distribution. However, excessive COD disturbance disrupted the highly efficient biofacies structure and microbial balance, which then decreased the ANAMMOX competitiveness. © 2013 Society of Chemical Industry

Nutrient Removal from Municipal Wastewater and Microbial Community Analysis of a Combined ABR‐MBR (CAMBR) Process

In order to reduce the external nutrient loads in Lake Taihu, it is known that controlling nutrient input is the most effective way of reducing the risk of blooms. In this regard, simultaneous nitrogen and phosphorus removal by using a combined system consisting of an anaerobic baffled reactor and an aerobic membrane bioreactor for municipal wastewater treatment under the operation condition (a hydraulic retention time of 7.5 h, a recycle ratio of 200% and dissolved oxygen of 3 mg/L) was investigated. The results demonstrated that the process had a good ability to adapt to the influent shift, and was able to achieve the simultaneous nutrient removal of both two kinds of municipal wastewaters effectively with the high removal efficiencies of chemical oxygen demand, ammonium, total nitrogen, and total phosphorus more than 89, 98, 65, and 83% respectively. Analysis of fluorescence in situ hybridization showed that ammonia‐oxidizing bacteria, nitrite‐oxidizing bacteria, and phosphorus accumulating organisms all slightly decreased may be due to the sudden drop of influent nutrient. In addition, the high sum of the three kinds of bacteria may be the underlying reason why the CAMBR process was efficient for nitrogen and phosphorus removal.

Hydrogenotrophic denitrification for tertiary nitrogen removal from municipal wastewater using membrane diffusion packed-bed bioreactor

A lab-scale membrane diffusion packed-bed bioreactor was used to investigate hydrogenotrophic denitrification for tertiary nitrogen removal from municipal wastewater. After start-up, the bioreactor had been operated for 165 days by stepwise increasing influent loading rates at 30 and 15°C. The results indicated that this bioreactor could achieve relatively high nitrogen removal efficiencies. The denitrification rates reached 0.250 and 0.230 kg N/(m(3)d) at 30 and 15°C respectively. The total nitrogen concentration in effluent was entirely below 2.0 mg/L at the steady operation state. The average increase of total organic carbon in effluent was approximately 0.41 mg/L, suggesting the risk of organic residue can be completely controlled. Dissolved oxygen (DO) did not show obviously negative effects on hydrogenotrophic denitrification. There was only slight decrease of DO concentration in effluent, which demonstrated almost all of the hydrogen was used for nitrate reduction.

Optimizing Control Strategy of Interactive Reactor with Simulation

Interactive reactor is a powerful and complicated wastewater treatment, it can fulfill various operation modes by changing inlet site, inner recycle site, return sludge site and DO concentration, therefore interactive reactor can adjust to different conditions and achieve steady and efficient wastewater treatment effect. This paper intended to establish integrated optimizing control strategy for better use of interactive reactor. In order to confirm the application boundary of typical operation mode, pilot-scale experiment and simulation were carried out. JHB and ALO modes were experimented for capacity evaluation and simulation calibration. A2/O, inverted A2/O and ALO modes were selected as representatives for the strategy establishment with simulation. According to the study results, inverted A2/O mode was suitable for the influent BCOD/TN value more than 5, A2/O mode was better choice for the influent BCOD/TN value between 4 and 5, and ALO can achieve high nitrogen removal efficiency treating wastewater with influent BCOD/TN between 3 and 4 by short-cut nitrification and denitrification. Contributed to the excellent treating effect of interactive reactor, several projects have been built and run well under the optimizing control strategy.

Isolation of an Aerobic Denitrifying Bacterial Strain from a Biofilter for Removal of Nitrogen Oxide

A strain of bacteria CP1 with high nitrogen removal efficiency was newly isolated from the biofilm of a biofilter for removal of NOx from flue gas. The isolate was identified as Pseudomonas aeruginosa based on its physiological and biochemical characteristics and the results of 16S rRNA gene homology analysis. The new isolate had a high denitrifying ability, removing 98.49% of the nitrate in a 24-h period under aerobic conditions, with no nitrite accumulation. With regard to the nitrogen balance, the percentage of nitrogen lost in the flask culture was estimated to be 32.3%, which was presumed to be converted to nitrogen gas. An analysis of its denitrification activity showed that the optimal C/N and temperature were 12 and 30°C-40°C, respectively. By using glucose, sodium citrate and succinate, CP1 removed nitrate with high denitrification efficiency. The change in DO did not influence the effects of denitrification when it varied from 0 to 7.2 mg/L. The results show that CP1 could be a good candidate for the process of aerobic denitrification.

Application Of Constructed Wetlands For The Nitrogen Removal

Copyright: © 2013 Xu-jie LU. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Nitrogen was a main contaminant of surface water in China presently. For instance, discharges of oil refining, chemical fertilizer, food and cultivation industry production have high concentration ammonia nitrogen in China, and the concentration of ammonia nitrogen is 200-6000 mg/L [1]. Uncontrolled discharge of nitrogen into natural water channels stimulates eutrophication of surface waters, such as lakes and rivers [1,2]. Thus, contamination of water bodies by nitrogen has led to increasing attention focused on wastewater treatment. As well, Nitrogen removal urgently needed to be solved as one of the difficult problem in the current wastewater treatment. At present, more and more researchers focused on simple and low-cost natural technologies (e.g constructed wetlands). As typical natural and environmental friendly systems [3,4] constructed wetlands primarily depend on naturally occurring energies such as solar radiation, and biomass storage, and using rooted water tolerant plants and media to provide treatment of wastewater [5,6]. As a green treatment technology and engineering measure, they have the unique advantage of producing higher effluent quality without the input of fossil energy thereby reducing operation costs and high costs of the maintenance [7-10]. Constructed wetlands are engineered wetlands with saturated or unsaturated substrates, a large biomass of plants, such as emergent, floating and submergent, and a large variety of microbial communities, like the as nitrifying bacteria denitrifying bacteria [6]. Compare with conventional technologies (such as membrane bioreactors), constructed wetlands have higher nitrogen removal efficiency and lower organic removal by increasing the contact time between wastewater and biofilms [11,12]. Besides, the characteristics of constructed wetlands are impacting resistance, stabilizing the effluent quality, simplifying operation and maintenance, and low operating cost. However, their floor spaces are larger, and the wetlands exhibit higher pollutant removal rates under greater loadings conditions [1]. In extremely cold areas, the phenomenon is more significant. The wastewater treatment technology of constructed wetlands is increasable widely used in urban and rural areas of South China [13-16]. However, constructed wetlands were not widespread uses of technology in China which spans 49 latitudes, resulting in the large differences in climate, terrain and plant communities between South and North China, especially low temperature under winter cold climate condition, such as in Northeast China. Hence, there are a lot of difficulties in adopting constructed wetlands in Northeast China.

Start-Up, Modelling and Simulation of the Anammox Process in a Membrane Bioreactor

There are certain well-known methods of diminishing concentrations of nitrogen compounds, but they are ineffective in case of nitrogen-rich wastewater with a low content of biodegradable carbon. Partial nitritation followed by anaerobic ammonium oxidation (Anammox) process appear to be an excellent alternative for traditional nitrification and denitrification. This paper presents the feasibility of successful start-up of Anammox process in a laboratory-scale membrane bioreactor (MBR). It was shown that the combination of membrane technology and Anammox process allowed to create a new highly efficient and compact system for nitrogen removal. It was possible to achieve average nitrogen removal efficiency equal to 76.7 ± 8.3%. It was shown that the start-up period of 6 months was needed to obtain high nitrogen removal efficiency. The applied biochemical model of the Anammox process was based on the state-of-the-art Activated Sludge Model No.1 (ASM 1) which was modified for accounting activity of autotrophs (nitrite-oxidising bacteria and nitrateoxidising bacteria) and anammox bacteria. In order to increase the predictive power of the simulation selected parameters of the model were adjusted during model calibration. Readjustment of the model parameters based on the critically evaluated data of the reactor resulted in a satisfactory match between the model predictions and the actual observations.

Minimization of nitrous oxide emission from anoxic–oxic biological nitrogen removal process: Effect of influent COD/NH4+ ratio and feeding strategy

Nitrous oxide (N(2)O) is an important greenhouse gas and biological nitrogen removal process of wastewater treatment plant is one of its sources. Mechanisms of N(2)O emissions from anoxic-oxic biological nitrogen removal process were investigated and minimizations of N(2)O emissions were carried out from the aspect of organic carbon supplement, i.e., influent COD/NH4+ ratio (C/N ratio) and feeding strategy. Results showed that during anoxic-oxic biological nitrogen removal process, most of the N(2)O emissions occurred during the oxic phase, and both nitrifier denitrification and aerobic hydroxylamine oxidation pathways were possible mechanisms responsible for N(2)O emissions. N(2)O conversion rate decreased from 6.0% to 1.3% when the influent C/N ratio was increased from 7.5 to 14.5. This was mainly because of decrease in the abundance of Nitrosomonas-like ammonia oxidizing bacteria. Step feeding and external carbon source addition could reduce N(2)O conversion rate by 66.6% and 12.0%, respectively. Both of them were feasible methods for minimizing N(2)O emission from wastewater treatment process. The low N(2)O emission of step feeding was because of its high dissolved oxygen (DO) and low ammonium concentrations during the oxic phase, while the minimization effect of external carbon source addition was ascribed to its high nitrogen removal efficiency.

Advanced Treatment of Wastewater from Food Waste Disposer in Modified Ludzack-Ettinger Type Membrane Bioreactor

This paper proposes a modified Ludzack-Ettinger (MLE) type membrane bioreactor (MBR) as a method of treatment for wastewater from food waste disposer. Micro-membrane filtration allows for an extremely low concentration of suspended solids in the effluent. The effluent of the reactor in question is characterized by a relatively high level of non-biodegradable organics, containing a substantial amount of soluble microbial products and biomass. Results obtained in this paper by measurement of membrane fouling are consistent with biomass concentration in the reactor, as opposed to chemical oxygen demand (COD). The MLE process is shown to be effective for the treatment of wastewater with a high COD/N ratio of 20, resulting in are markedly high total nitrogen removal efficiency. Denitrification could be improved at a higher internal recycle ratio. Despite the low concentration of influent phosphorus, the phosphorus concentration of the outflow is seen to be relatively high. This is because outflow phosphorous concentration is related to COD consumption, and the process operates at along solids retention time.

High-rate denitrification using polyethylene glycol gel carriers entrapping heterotrophic denitrifying bacteria

This study evaluated the nitrogen removal performance of polyethylene glycol (PEG) gel carriers containing entrapped heterotrophic denitrifying bacteria. A laboratory-scale denitrification reactor was operated for treatment of synthetic nitrate wastewater. The nitrogen removal activity gradually increased in continuous feed experiments, reaching 4.4 kg N m−3 d−1 on day 16 (30 °C). A maximum nitrogen removal rate of 5.1 kg N m−3 d−1 was observed. A high nitrogen removal efficiency of 92% on average was observed at a high loading rate. In batch experiments, the denitrifying gel carriers were characterized by temperature. Nitrate and total nitrogen removal activities both increased with increasing temperature, reaching a maximum at 37 and 43 °C, respectively. Apparent activation energies for nitrate and nitrite reduction were 52.1 and 71.9 kJ mol−1, respectively. Clone library analysis performed on the basis of the 16S rRNA gene revealed that Hyphomicrobium was mainly involved in denitrification in the methanol-fed denitrification reactors.

Fate and effect of benzalkonium chlorides in a continuous-flow biological nitrogen removal system treating poultry processing wastewater

Quaternary ammonium compounds (QACs) are used for sanitation in many poultry processing facilities. This work investigated the fate and effect of a mixture of benzalkonium chlorides (BACs), a class of QACs widely used in commercial antimicrobial formulations, on the biological nitrogen removal (BNR) processes. A laboratory-scale BNR system was operated continuously for 670days, fed with poultry processing wastewater amended with a mixture of BACs. Initially, the nitrogen removal efficiency deteriorated at a BAC feed concentration of 5mg/L due to the complete inhibition of nitrification. However, after 27days of operation, the system recovered and achieved 100% ammonia removal. High nitrogen removal efficiency was achieved even after the feed BAC concentration was stepwise increased up to120mg/L. Batch nitrification assays performed before, during, and after BAC exposure, showed that rapid microbial acclimation and BAC biodegradation contributed to the recovery of nitrification achieving efficient and stable long-term BNR system operation.

Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge

Taking advantage of the good biocompatibility and high efficiency of nitrogen removal with microbes, nitrifying and denitrifying bacteria, are becoming increas- ingly more widely used for wastewater treatment and prevention of eutrophication. In this research, an aerobic nitrifying-denitrifying bacterium was successfully screened from activated sludge and identified as Pseudomonas sp. (CCTCC No M2010209) by the 16S rDNA sequence. The activity verification confirmed its nitrifying-denitrifying capability of removing ammonium, nitrate and nitrite nitro- gen. The types of carbon sources and carbon-nitrogen ratio greatly influenced the removal efficiency of NH4 + -N and NO3 � -N. When the initial concentrations of NH4 + -N and NO3 � -N in synthetic wastewater were less than 70 and 50 mg/L, the nitrogen removal rates reached 94 and 90% in 9 h, respectively. Preliminary comparisons of nitrogen removal capacity between this isolate and other commer- cial preparations in the treatment of synthetic wastewater revealed its promising potential to be used in the actual wastewater treatment.

Anaerobic ammonium oxidation (Anammox) in immobilized activated sludge biofilms during the treatment of weak wastewater

This work studied the formation of molecular nitrogen by the microbial population of immobilized activated sludge of the domestic wastewater treatment plants (WWTP) that employ the technology developed by ZAO ECOS Company. The technology includes physicochemical water pretreatment and treated water recycling. A hard flexible fibrous brush carrier is used for the immobilization of microorganisms. The presence of both aerobic and anaerobic microorganisms and functioning of the methanogenic microbial community was shown in the biofilms developing on the carrier fibers and in suspended sludge. The high efficiency of nitrogen removal at a low C/N ratio was established to be due to the conjugated nitrification, denitrification, and anammox processes, whose functioning was demonstrated by laboratory cultivation methods and by studying the processes in batch and continuous reactors. Fluorescence in situ hybridization with 16S rRNA-targeted oligonucleotide probes (FISH) revealed bacteria belonging to the order Planctomycetales, particularly their anammox group. This work is the first evidence of the important role of the anammox process in the combined system of physicochemical and biological treatment of weak wastewater (BCDEAMOX).

Performance of Denitrifying Phosphorous Removal Process Employing Nitrite as Electron Acceptor and the Characterization of Dominant Functional Populations

A sequencing batch reactors (SBR) was adopted to investigate the denitrifying phosphorus removal efficiency employing nitrite as electron acceptor under anaerobic/anoxic condition. The experimental results showed that high nitrogen and phosphorus removal efficiency could be obtained under the following conditions: nitrite concentration of 30~40 mg/L, COD concentration of 400 mg/L, pH 8.0±0.2 in anaerobic stage and pH 7.2±0.2 in anoxic stage, sludge retention time (SRT) of 22 days. When the reactor performed steadily, a dominant functional strain was screened from the activated sludge, which has high nitrite and phosphorus removal efficiency. Batch tests results showed that the removal degree of nitrite and phosphorus could reach 99.18% and 84.94% respectively when their concentrations were 20mg/L and 10mg/L. according to the morphology and physio-biochemical characteristics, and the results of 16S rDNA sequencing, it is determined that the strain belongs to the Genus of Sphingobacterium. The experimental results achieved in this study might offer guidance to the development of shortcut denitrifying phosphorus removal process.