This study evaluates water and porcine vitreous flow rates and duty cycle using the Millennium Vitrectomy Enhancer (MVE) system (Bausch & Lomb, St. Louis, MO). A precision balance measured mass of water or vitreous removed from a vial within a certain time by 20-, 23-, and 25-gauge MVE cutters at 800, 1,000, 1,500, 2,000, and 2,500 cuts per minute (CPM) with various aspiration levels was studied. Frame-by-frame analysis of high-speed video was used to determine duty cycle. Larger cutter and higher aspiration levels produced greater flow rates. Water flow rate showed a parabolic trend peaking at 1,500 CPM and dropping moderately and vitreous flow rate increased moderately with cut-rate increased. The MVE system maintained a high flow rate and high duty cycle even at high cut-rates. Flow rates for the MVE system are stable and predictable for all cutter gauges, which should aid the surgeon to select the optimal parameters for vitrectomy.