Many aquatic animals exhibit diurnal periodicity in activity. In lakes the daily vertical migrations of crustaceans, rotifers, and the insect Chaoborus have been the subject of many studies (Bainbridge 1961, Pennak 1944, Berg 1937). Although similar phenomena might be expected in streams, little has been reported. Moon (1940), who studied the colonization by invertebrates of bottom materials set out in trays, reported greater activity at night than during the day in both a lake and a stream. It is reasonable to postulate that changes in activity among stream organisms would be manifested in changes in the drift rate (defined as the quantity of organisms drifting downstream per unit time per unit of stream width), but published studies on drift either have not undertaken measurement of drift rates during hours of darkness or have not distinguished between night and daytime drift rates (Needham 1928, Lennon 1941, Dendy 1944, Mullor 1954, Waters 1961). However, Lennon (personal communication) observed in his study greater activity of stream insects at night and believed that in his 24-hour drift samples the immatures were collected mostly at night. Also, in a personal communication, G. R. Alexander, Michigan Department of Conservation, stated that in his drift studies (as yet unpublished), there appeared to be peaks in drift rate in the evening and again in early morning. Prior to the present study, ecological investigations in the summer of 1959 included drift measurements out of an exclosure set into a stream bottom; much higher drift rates were observed at night than during daylight hours. Subsequent observations, both from an exclosure and in the open stream, confirmed the daily changes as regularly occurring events. The purpose of the present study was to document more intensively this phenomenon of diurnal periodicity in the drift rate. I would like to express appreciation to Robert J. Knapp for his conscientious assistance in the field; to many students, especially George A. Swanson, for the extensive work of sorting and analyzing the samples in the laboratory; and to Roy C. Arnold on whose property the field work was conducted, and who generously offered his cooperation and facilities. The study was assisted by National Science Foundation research grant No. F 9935.