In this research, extensive testing results of the specimens of HRB500 high-strength steel rebars joined by flash butt welding were demonstrated, including metallographic and micro-hardness tests, monotonic tensile tests, high-cycle fatigue tests, and fracture surface analysis. The chief aim of the present research is to investigate the applicability of flash butt welding for HRB500 high-strength steel rebars. Thus, these tests were executed on the welded specimens, which were previously manufactured to evaluate the tensile and high-cycle fatigue properties. Meanwhile, a series of experimental technical details were proposed in the test process. Some conclusions can be obtained as follows: welding heat can change the metallographic structures of HRB500 high-strength steel rebars, and the welding heat-affected zone on the steel rebars with diameter of 16 mm is totally about 10 cm in length. Besides, the deformability and tensile strength of the welded specimens decline with their diameter degrees increasing, and brittle fractures occur in those welded specimens with diameter of 32 mm. Furthermore, three-parameter S-N curves of the welded specimens and the steel rebars base materials are obtained based on the fatigue test results. Fatigue strengths of welded specimens decrease with their diameters increasing, and reduction of high-cycle fatigue strength is more apparent for large-diameter welded specimens. Welded specimens are more prone to pre-mature fatigue failure than base materials of HRB500 high-strength steel rebars, and the rate of change in fatigue strength of welded specimen decreases with the increase of loading cycles. At last, mechanism of crack propagation in fatigue process is analyzed and further evaluated by fracture surface analysis with the aim of wider application.