Highest Flavonoid Content Research Articles

Application of Fe2O3 nanoparticles improves the growth, antioxidant power, flavonoid content, and essential oil yield and composition of Dracocephalum kotschyi Boiss.

Dracocephalum kotschyi Boiss., an endemic and endangered medicinal and aromatic plant in Iran, showcases distinct botanical characteristics and therapeutic promise. According to the IUCN grouping criteria, this plant is facing challenges due to overcollection from its natural habitats. To address this issue, there is an increasing inclination towards cultivating this species within agricultural systems. This study aimed to evaluate the impact of applying Fe2O3 nanoparticles (NPs) at varying concentrations (50, 100, and 200 mg L-1), as well as bulk Fe2O3 at the same concentrations, on the growth, essential oil production, antioxidant capacity, total phenol, and flavonoid content of D. kotschyi. The foliar application of 100 and/or 200 mg L-1 of Fe2O3 NPs resulted in the greatest leaf length and dry weight, while Fe2O3 NPs at the level of 100 mg L-1 led to the highest leaf/stem ratio. Additionally, spraying 200 mg L-1 of Fe2O3 NPs and all concentrations of bulk Fe2O3 positively impacted chlorophyll and carotenoid levels. Both nano and bulk Fe2O3 supplements stimulated H2O2 production and subsequently enhanced enzymatic antioxidant activity. The use of 50 mg L-1 of Fe2O3 NPs resulted in the highest flavonoid content and non-enzymatic antioxidant activity. Meanwhile, the highest essential oil content and yield was achieved by the application of 50 and/or 100 mg L-1 Fe2O3 NPs. The addition of low concentration of Fe2O3 NPs (50 mg L-1) resulted in a significant increase in the concentration of geranial, while a higher supply of Fe2O3 NPs (200 mg L-1) significantly decreased the percentage of neral in the essential oil. Overall, the application of Fe2O3 NPs demonstrated significant potential for increased biomass, enhanced yield, essential oil production, and phytochemical attributes. The findings highlight the versatility of Fe2O3 NPs at optimal concentrations, acting as both a nano-fertilizer and a nano-inducer, promoting the production and accumulation of valuable secondary metabolites in plants.

Optimasi Metode Microwave-Assisted Extraction (MAE) untuk Menentukan Kadar Flavonoid Total Ekstrak Etanol Kulit Pisang Kepok (Musa Paradisiaca.L)

Indonesia is a country that has a lot of banana diversity. One of the banana plants that is widely found in Indonesia is the Kepok banana (Musa Paradisiaca.L). Kepok banana skin has a very high content of flavonoids and phenols. Flavonoids are known as good antioxidants because they can find reactive oxygen species with the phenolic hydroxyl group that flavonoids have. The aim of this research was to determine the results of the optimization of the Microwave-Assisted Extraction (MAE) method on the total flavonoid content of kapok banana peel ethanol extract. Microwave-Assisted Extraction (MAE) was done to extraction flavonoid from ethanol extract of kepok banana peel (Musa Paradisiaca.L). The sample collected from Widoro village, Karangsambung sub-district, Kebumen regency. Optimum extraction condition was determined by the Response Surface Methodology (RSM). The Box- Behnken design (BBD) was used to evaluate the influence of 3 factors with 3 levels extraction that is power level (10%, 30%, 50%), ethanol concentration (50, 70, 96%), and extraction time (3, 5 and 7 minutes) with 15 different runs. The research showed that optimum extraction condition was at 24,14 % of power level, 66,26% ethanol concentration, and time extraction of 3,52 minutes with calculations from the Design Expert program, the total flavonoid content response was 8.491 Mg QE/g. The total flavonoid content value obtained experimentally was 8,246 Mg QE/g under condition of 30 % power level, 70% ethanol concentration, and time extraction of 5 minutes. Based on the result, total flavonoid content can be significantly increased by optimizing the MAE process use RSM.

Phytochemical Analysis and In Vitro Evaluation of Antidiabetic Potential of Banana Bract Lehya

Banana Bract Lehya, a traditional Ayurvedic formulation, has gained recognition for its potential therapeutic applications. This study aimed to prepare and evaluate the physicochemical properties and biological activities of Banana Bract Lehya. The lehya was prepared using banana bract powder (Musa paradisiaca), jaggery, tamarind, and a blend of spices including cumin, ajwain, ginger, fennel, and black pepper. Phytochemical screening revealed the presence of various bioactive compounds, with high concentrations of flavonoids and phenolic compounds, and moderate levels of tannins and terpenoids. Physicochemical analysis showed a pH of 6.20, loss on drying of 70%, total ash content of 3.2%, and total solid content of 96%. Organoleptic evaluation indicated a light brown and black color, pungent and spicy taste, and jelly-like texture. The total phenolic content was 78.5 ± 3.2 mg GAE/g, while the total flavonoid content was 42.3 ± 2.1 mg QE/g. Antioxidant activity, assessed by DPPH free radical scavenging assay, showed an IC50 value of 127.6 ± 5.4 μg/mL, compared to ascorbic acid's 23.8 ± 1.2 μg/mL. Glucose adsorption capacity increased with rising glucose concentration, suggesting potential anti-diabetic effects. The lehya exhibited concentration-dependent inhibition of α-amylase and α-glucosidase enzymes, with IC50 values of 156.3 ± 7.2 μg/mL and 143.7 ± 6.5 μg/mL, respectively, compared to acarbose's 83.2 ± 3.8 μg/mL and 95.6 ± 4.1 μg/mL. These findings provide valuable insights into the composition, physicochemical properties, and potential health benefits of Banana Bract Lehya.

A-064 Improved Insulin production in Advanced glycation end-products (AGEs)-induced RIN-m5F pancreatic β-cells by using a Flavonoid-Rich Medicinal Plant, Kaempferia parviflora treatment

Abstract Background Advanced glycation end-products (AGEs) are glycated proteins that react with reducing sugar which causes the damages of function and cellular signaling of insulin production in insulinoma cells. AGEs also contribute to hyperglycemia-induced insulin resistance in type 2 diabetes. AGEs gradually form during the chronic high blood glucose condition, which is a major risk factor and harmful to β-cells function and survival, and could alter the insulin signaling and downregulate the insulin-responsive transcription factors including PDX-1, Glut-2, GCK, and Pre-INS. Kaempferia parviflora, also known as black ginger and flavonoid-rich medicinal plant, has beneficial properties for type 2 diabetes patients as a counteracts the detrimental effects of AGEs and improves the insulin production in pancreatic β-cells. Thus, we hypothesize that treated β-cells with K. parviflora, which is the most effective flavonoid-containing plant might protect against the damage of AGEs-induced insulin impairment, and these effects are also responsible for improved insulin synthesis and the essential transcription factors in pancreatic β-cells. Methods Rat pancreatic β-cell line RIN-m5F (ATCC CRL-2058) were maintained and cultured in RPMI 1640 medium containing 10% (v/v) FBS, 1% penicillin-streptomycin at 37◦C in a humidified atmosphere containing 5% CO2. RIN-m5F cells were pre-treated with the K. parviflora at concentrations 0, 25, and 50 µg/ml for 48 hours, and afterward, exposure to 250 µg/ml AGEs treatment. To quantify the bioactive compound of K. parviflora, total phenolic and flavonoid content assays were performed and the transcriptional changes of insulin-related genes (PDX-1, Glut-2, GCK, and Pre-INS) were determined by using real-time qRT-PCR. Results K. parviflora has shown a high phenolic content and flavonoid content was 50.43 ± 1.74, 26.66 ± 1.18 mg GAE/g Dry weight of sample, respectively. For the transcriptional changes of insulin-related genes, the levels of gene expression in the regulators of insulin secreting pathway (PDX-1, Glut-2, GCK, and Pre-INS) were significantly downregulated after exposure to AGEs-treated, while the pre-treatment of K. parviflora was showed statistically difference. PDX-1, Glut-2, GCK, and Pre-INS expressions were significantly upregulated after pre-treated with 50 µg/ml K. parviflora. However, under the AGEs-treated alone condition, the transcriptional levels were significantly decreased as compared to the control (untreated). Taken together, these results suggest K. parviflora could be a potential natural medicine to ameliorate the damage of insulin production in AGEs-induced β-cell damage and highlight promising alternatives for therapeutic avenue against diabetes. Conclusions Treated RIN-m5F pancreatic β-cells with K. parviflora, a flavonoid-rich medicinal plant, could upregulate the insulin-responsive transcription factors and improve the insulin production in damaging AGEs-induced pancreatic β-cells. Our findings provide a novel strategy related metabolic disease and insights into the regulation of insulin synthesis by using K. parviflora treatment as a therapeutic agent against type 2 diabetes.

Exploring the chemical composition and biological effects of four Salvia hybrids: an innovative perspective on functional yields

AbstractGenus Salvia is considered one of the largest genera of family Lamiaceae with many recorded species and hybrids of valuable biological importance. This study involved the phytochemical characterization of four Salvia hybrids (KNM23 (S. fructicosa × S. officinalis), KNM101 (S. aramiensis × S. officinalis), KNM5 (S. aramiensis × S. officinalis) and KNM12 (S. fructicosa × S. officinalis)) through measuring their total phenolic content (TPC) and total flavonoid content (TFC) together with profiling through UPLC/MSn analysis followed by investigation of their antioxidant activities and enzyme inhibitory potential through different techniques. Hybrid KNM23 presented significantly high concentration of both phenolic and flavonoid, displaying a TPC of 92.10 mg GAE/g and a TFC of 50.85 mg RE/g. The UPLC/MSn profiling resulted in the tentative identification of one hundred eighty components from different phytochemical classes mainly flavonoids, phenolic acids, phenyl propanoids, tannins and many others. The antioxidant assay showed that hybrid KNM23, a hybrid of S. fruticosa and S. officinalis, exhibited the highest antioxidant activity in the DPPH, ABTS, and PBD assays, measuring 436.38 mmol TE/g, 543.65 mmol TE/g, and 3.20 mmol TE/g, respectively. KNM23 and KNM12 showed no inhibition of AChE and α‐glucosidase. However, KMN12 demonstrated highest BChE and tyrosinase 2.57 mg GALAE/g and 12.91 mg KAE/g, respectively. Our results suggest that the Salvia hybrids can be considered as natural sources of bioactive compounds in the development of health‐promoting applications.

Active Components and Skin Care Mechanism of Sea Grape (Caulerpa lentillifera) Extract

This study successfully obtained sea grape extract through mechanical extraction methods, which is rich in active components. These primary active ingredients include high concentrations of crude polysaccharides, total phenolics, and flavonoids. Using two zebrafish models, we observed significant skin moisturizing and repair effects of this extract. Further experiments demonstrated its remarkable anti-aging and skin tightening effects through the DPPH method and elastase inhibition assay. Additionally, the extract showed significant anti-inflammatory effects in a model built on 3 dpf (days post fertilization) Tg (corola: EGFP) transgenic zebrafish. The extract’s safety and reliability were confirmed through a final dermal application test on rabbits. Furthermore, we also analyzed the possible mechanism of Caulerpa lentillifera extract skin care. Overall, our research indicates that sea grapes hold tremendous potential as a novel natural ingredient for skincare. It is foreseeable that sea grapes will become an indispensable component in the future medical skincare market.

Bioefficacy of Methanolic Leaf Extracts from Coleus amboinicus and Mentha spicata against Aedes aegypti Mosquitoes

Mosquito-borne diseases (MBDs) pose significant public health challenges globally, with India and Sub-Saharan Africa accounting for the majority of cases. Aedes aegypti, a primary vector of dengue fever, thrives in an environment with inadequate water supply and poor waste management. The increasing resistance of mosquito population to chemical insecticides necessitates the exploration of alternative control strategies, such as botanical extracts. This study evaluates the adulticidal efficacy of methanolic leaf extracts of Coleus amboinicus and Mentha spicata against the adults of Ae. aegypti. Fresh leaves of both plants were collected, dried, and extracted with methanol. Phytochemical screening revealed a moderate presence of alkaloids, flavonoids, saponins, terpenoids, and glycosides in C. amboinicus, while phenols are highly prevalent, indicating significant biological activity. In contrast, M. spicata displayed a high concentration of alkaloids, flavonoids, and terpenoids, though saponins, phenols, and glycosides were less abundant. Adulticidal bioassays demonstrated that C. amboinicus achieved higher mortality rates across all concentrations compared to M. spicata. The LC50 value for C. amboinicus was 202.00 ppm, while M. spicata exhibited an LC50 of 230.00 ppm. Similarly, the LC90 values were 428.51 ppm and 457.40 ppm for C. amboinicus and M. spicata, respectively. These findings suggest that C. amboinicus has a more balanced and diverse phytochemical profile, which likely contributes to its superior adulticidal activity against Ae. aegypti. This study highlights the potential of C. amboinicus as a natural alternative for mosquito control, warranting further exploration and development for effective vector management.

Bioguided isolation of anti-inflammatory and anti-urolithiatic active compounds from the decoction of Cissus gongylodes leaves

Ethnopharmacological relevanceThe Cissus gongylodes has traditionally been used in the diet of indigenous people in Brazil and in traditional medicine for kidney stone removal and inflammatory diseases. The active compounds responsible for these pharmacological activities are unknown. Aim of the StudyThis study aims to isolate, for the first time, the compounds in the decoction of C. gongylodes leaves responsible for their anti-inflammatory and anti-urolithiatic ethnopharmacological properties. Materials and MethodsThe most active fractions of the C. gongylodes leaf decoction were fractionated using SPE-C18 and the compounds were purified through HPLC-UV-DAD. The decoction fractions and isolated compounds were evaluated for their anti-inflammatory and anti-urolithiatic activities. The anti-inflammatory activity was assessed using an ex vivo assay in human blood induced by LPS and calcium ionophore, measuring inflammatory mediators, PGE2 and LTB4. The anti-urolithiatic activity was evaluated using an in vitro experimental model with human urine to determine the dissolution of the most recurrent calcium oxalate (CaOx) crystals. Additionally, the decoction was chemically characterized through metabolomic analysis using UHPLC-ESI-HRMS. ResultsThe isolated compounds from the decoction of C. gongylodes, including rutin, eriodictyol 3’-O-glycoside, and isoquercetin, have demonstrated significant multi-target actions. These components act as anti-inflammatory agents by inhibiting the release of main inflammatory mediators, PGE2 and LTB4. Additionally, they exhibit anti-urolithiatic properties, promoting the dissolution of calcium oxalate (CaOx) crystals. Furthermore, the characterization of the decoction by UHPLC-ESI-HRMS revealed a high content of flavonoids, mainly glycosylated flavonoids. ConclusionsThe results support the traditional use of C. gongylodes decoction, identifying the compounds responsible for its anti-inflammatory and anti-urolithiatic effects. The decoction fractions and isolated compounds exhibited dual anti-inflammatory activity, effectively inhibiting key inflammatory pathways and potentially presenting fewer adverse effects while also promoting the dissolution of CaOx crystals associated with urolithiasis. The multi-target action displayed by C. gongylodes is particularly desirable in the treatment of urolithiasis, as inflammation and PGE2 production precede and contribute to the formation of CaOx crystals in the kidneys. Based on these actions, C. gongylodes emerges as a potent source of active compounds for the development of new treatments for urolithiasis.

Extraction of Flavonoids from Parasitic plant Macrosolen cochinchinensis using Ultrasound-Assisted Extraction: An Optimization Approach

The parasitic plant Macrosolen cochinchinensis (Lour.) VAN Tiegh, commonly found parasitizing mango trees, contains flavonoid compounds with potential anticancer properties. This study aims to optimize the extraction of flavonoids from M. cochinchinensis using the Ultrasonic Assisted Extraction (UAE) method. Three extraction parameters were investigated to determine the best conditions for maximizing extract yield and flavonoid concentration. The parameters considered for the UAE technique were different ethanol concentrations (30%, 70%, and 96%), extraction times (15, 30, and 45 minutes), and solvent-to-sample ratios (1:10, 1:20, and 1:30). The study used Response Surface Methodology (RSM) to identify the optimal extraction conditions. The analysis using RSM indicated that the highest extraction yield (10%) was achieved with a sample-to-solvent ratio of 1:30, 30% ethanol concentration, and an extraction time of 45 minutes. The highest flavonoid content (457.96 mg QE/g extract) was obtained with a solid-to-liquid ratio between 1:20 and 1:30, using 65 to 80% ethanol solvent and an extraction time of 45 minutes. These results suggest that these parameters extract flavonoid compounds from M. cochinchinensis leaves.

ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES OF BRASSICA JUNCEA EXTRACTS AND SILVER NANOPARTICLES: A SYNERGISTIC APPROACH AGAINST ESCHERICHIA COLI

Brassica juncea, a plant known for its anticancer and antioxidant characteristics, is rich in glucosinolates, flavonoids, carotenoids, and phenolic acids. AEBJ exhibited a high concentration of total flavonoids, measuring 201.09 ± 0.232 mg/g in terms of quercetin equivalents. However, it had a relatively lower total phenolic content, measuring 49.43 ± 1.114 mg/g in terms of tannic acid equivalents. The structural characterisation of silver nanoparticles was performed using UV-Visible spectroscopy, followed by the study of these nanoparticles utilising surface plasmon resonance (SPR) measurements. The interaction between silver ions and extracts of Brassica juncea was seen, and the presence of AgNPs was verified through the detection of a visible peak at 425 nm. The Silver Nanoparticle (BJ 1) effectively suppressed the generation of DPPH radicals within the concentration range of 10-100 µg/ml. The linear regression coefficients for this inhibition were calculated to be 0.9869 and 0.9488, respectively. The IC50 values for Silver Nanoparticle (BJ 1) and BHT were 45.413 and 50.173, respectively. Silver Nanoparticle (BJ 1) exhibited a decrease in the conversion of ferricyanide to ferrocyanide as the concentration increased from 50-250 µg/ml. This was accompanied by an increase in the absorption of green light at a wavelength of 700 nm. The investigation into the antibacterial activity of silver nanoparticles (BJ 1) and Brassica juncea extracts against Escherichia coli has demonstrated substantial potential as antimicrobial agents. The findings indicate that both drugs demonstrate inhibitory effects on the growth of E. coli, with the concentration levels playing a substantial influence. This implies a mutually beneficial interaction between the two drugs, which could potentially amplify their efficacy in fighting bacterial infections. Greater doses of Brassica juncea extract exhibited bigger areas of inhibition, so validating its bioactive activities against pathogenic microorganisms. Furthermore, the Silver nanoparticle (BJ 1) had a potent antibacterial impact as a result of their capability to disturb bacterial cell membranes and impede metabolic activities. The combination of these two medicines has the potential to enhance therapy efficacy in clinical settings. Subsequent investigations should delve into the mechanisms underlying their antibacterial properties and evaluate their safety profiles for future medicinal uses. KEYWORDS: Brassica juncea; Silver Nanoparticles; Antioxidant Activity; DPPH; Reducing Power Assay; Antimicrobial Activity; Escherichia coli

Physicochemical Characterization and Antioxidant Activity of Algerian Bee Pollens: Exploring Geographical Influences

Abstract: This research study offers a comprehensive physicochemical analysis and assessment of antioxidant activity for 15 bee pollen samples collected from diverse regions of Algeria. The analysis included a wide range of parameters such as moisture content, sugar composition (fructose, glucose, and sucrose), ash, electrical conductivity, free acidity, pH, total flavonoid (TFC) and phenolic (TPC) contents, as well as protein and lipid contents. Additionally, the study evaluated the antioxidant activity using two methods: DPPH radical scavenging activity and ferricreducing antioxidant power (FRAP).The results revealed higher values of pH, free acidity, and electrical conductivity, indicating potential variations in the physicochemical properties of bee pollen samples from different regions. Notably, the samples were further distinguished by high total phenolic content (26.23 mg GAE/g), flavonoid content (4.73 mg QE/g), and protein content (21.7%), along with a unique mineral composition. The analysis also demonstrated the highest total antioxidant capacity and a marked correlation with total phenolics. This study highlights the influence of geographical factors on the physicochemical properties and antioxidant activity of Algerian bee pollen. Regional variations underscore the importance of considering geographical factors in evaluating bee pollen quality, nutritional value, and potential health benefits of Algerian bee pollen. This emphasizes their crucial role in functional foods and natural health products. Understanding these variations supplies valuable insights for optimizing the utilization of bee pollen in functional foods, dietary supplements, and pharmaceutical formulations. Further research into environmental factors influencing bee pollen's antioxidant properties could enhance our understanding of its therapeutic potential. other: Further research into environmental factors influencing bee pollen's antioxidant properties could enhance our understanding of its therapeutic potential. Additionally, exploring the potential applications of Algerian bee pollen in functional foods, dietary supplements, and pharmaceutical formulations could provide opportunities for product development and innovation in the food and healthcare industries.

Phytochemical Screening of Genus Primula Species Growing in Georgia and Study of their Antioxidant and Anti-inflammatory Potentials

Background: In traditional medicine, species of the genus Primula L. are used to treat various health conditions, such as eye disorders, respiratory infections, headaches, epilepsy, insomnia, as expectorants, and to promote wound healing. Objective: The goal of this article was to evaluate the antioxidant and anti-inflammatory activities of three species from genus Primula L. growing in Georgia: Primula macrocalyx, Primula woronowii and Primula saguramica. Methods: Initially, fractions containing both aerial and underground parts were air-dried, ground, and extracted with 80% ethanol. The extract was then concentrated by condensation and further dried through freeze-drying. Subsequently, additional chromatographic separations were carried out on Diaion HP-20 using solvents such as water, methanol (50% and 100%), and 100% ethyl acetate to isolate the desired fractions. To identify flavonoids and triterpene glycosides, the study employed thin-layer chromatography (TLC) alongside preliminary phytochemical tests. The antioxidant activity of these species was estimated in vitro by cell-free systems using ABTS and DPPH assays. The extracts' anti-inflammatory properties were evaluated using an ex-vivo cell system that isolated neutrophils. The study examined the extracts' impact on reactive oxygen species (ROS) production in neutrophils stimulated with PMA, as well as their effect on the catalytic activity of myeloperoxidase (MPO), a marker of inflammation in neutrophils. Results: Flavonoids and triterpene glycosides were primarily identified in the 50% and 100% methanol (MeOH) fractions of Primula species through TLC and preliminary phytochemical tests. For each experiment, gallic acid and quercetin served as standards at a concentration of 1 mg/ml, while the tested samples were prepared at concentrations of 5 mg/ml. Based on the IC50 findings, P. w 3 exhibits the most potent antioxidant and anti-inflammatory properties, as evidenced by the following indicators: ABTS - IC50=8.51 ± 0.18; DPPH - IC50=34.57 ± 0.47; PMN - IC50=0.68 ± 0.04; SIEFED - IC50=1.49 ± 0.7; and classical IC50=1.89 ± 0.01. Conclusion: Among the tested fractions, only the fraction of the 3 species prepared in MeOH (50%) showed the best dose-dependent antioxidant and anti-inflammatory activities, especially P.w 3 which is probably related to the high flavonoid content found in this species.

Choosing the optimal technology for obtaining Crocus alatavicus extract with a high content of flavonoids and carotenoids

In recent years, there has been a significant increase in demand for herbal medicines, due to the growing interest of consumers in natural and safe alternatives to synthetic medicines. In response to these trends, manufacturers are focusing on developing new formulas and extraction technologies to meet the increased demand for herbal medicines. Modern extraction methods make it possible to efficiently extract secondary metabolites from plant raw materials, maintaining an almost complete chemical composition and ensuring a high yield of extractives. The possibility of regulating the concentration of extracted biologically active substances during the process opens up new prospects for the use of plant raw materials as basic pharmaceutical substances. It was found that all the studied plant species of the genus Crocus L. demonstrate antioxidant, anti-inflammatory and cardioprotective activity. Crocus alatavicus, which is a representative of the flora of Kazakhstan, is of particular interest. The purpose of this study is to select the optimal extraction method for obtaining extracts with the maximum content of flavonoids and carotenoids from the aboveground part of Crocus alatavicus. The study of the technological parameters of obtaining an extract from this plant active substance is of practical importance for the further development of medicines and the formation of a scientific justification for their use.

Antioxidant and antidiabetic activities of Zingiber officinale var. Rubrum extracted with natural deep eutectic solvents

Polyphenols and flavonoid compounds are known to have antioxidant activity and can reduce blood glucose levels. Zingiber officinale var. Rubrum is one of the plants with high polyphenol content. This study aimed to extract phenolic and flavonoid compounds from this plant with various solvents primarily natural deep eutectic solvents (NADES), namely 70% ethanol, NADES1 [citric acid: sucrose (1:1)], NADES2 [sucrose: glucose: fructose (1:1:1)], NADES3 [choline chloride: glycerol (1:2)], and NADES4 [glycerol: urea (1:1)]. The phenolic and flavonoid levels were determined using spectrophotometer, the antioxidant activity was measured using DPPH and Ferric Reducing Antioxidant Power (FRAP) methods, while the antidiabetic activity was evaluated using enzymatic assay of α -amylase and α-glucosidase enzymes. The findings indicated that all NADES outperformed 70% ethanol in extracting phenols, with NADES1 showing the highest phenolic content. Meanwhile, the highest flavonoid content was observed in extracts with 70% ethanol and NADES1. The results of antioxidant activity analysis using DPPH revealed that extracts with 70% ethanol, NADES1, and NADES4 exhibited considerable strength (IC50: 45-49 µg/mL), with the average values of the three solvents being not significantly different. From the results of the FRAP assay, NADES3 demonstrated the highest strength Antioxidants Activity Index (AAI: 29.24±3.08 mmol/g), whereas 70% ethanol showed the lowest strength (AAI: 22.76±5.23 mmol/g). The average AAI values for all solvents indicated no significant differences. Among the solvents, NADES1 exhibited very strong inhibitory activity against α-amylase (19.62±0.20 µg/mL), while for α-glucosidase, NADES1 was stronger (IC50: 57.36±6.08 µg/mL) than other solvents. In conclusion, NADES can be a promising tool for extracting secondary metabolites from Zingiber officinale var. Rubrum as it shows both antioxidant and antidiabetic activities.

Phytochemical contents and antioxidant activity Paliurus spina-christi Miller leaf and seed extracts: PASS predictions, in silico studies on xanthine oxidase and cytochrome P450 1A1

Paliurus spina-christi Miller (PSC) is a shrub plant with important biological activities. For this reason, the phytochemical and biological activities of the PSC leaf and seed extracts were investigated in our study. This study performs phytochemical analyses (total phenol and flavonoid content, LC-ESI-MS/MS, and GC-MS/MS) and bioactivity assays (antioxidant) for the PSC leaves and seeds. In silico study and PASS prediction of main compounds in LC-ESI-MS/MS and GC-MS/MS analysis were also investigated. The leaf extract showed a high total phenolic and flavonoid content. In addition, the contents of hesperidin (25.548 mg/g extract) were high in the LC-ESI-MS/MS and GC-MS/MS analyses. It was noted that the leaf extract's antioxidant activities were higher than standard. The molecular docking of hesperidin with xanthine oxidase and cytochrome P450 1A1 had high MolDock score (-179.68, -149.156) and binding energy (-11.40 kcal/mol, -9.90 kcal/mol), respectively. This investigation pioneered using PSC leaf extracts as food supplements and medicine.

Antioxidant and Biological Activity of Mexican Madroño Fruit (Arbutus arizonica).

The fruit of the Mexican madroño (Arbutus arizonica) has been consumed since pre-Columbian times by North American tribes and native groups in Mexico. Despite this, reports on its chemical composition and biological activity are limited. This work aims to determine the antioxidant, antiproliferative, and digestive enzyme inhibition activities of the methanol amberlite-retained extract of Mexican madroño. Results showed that madroño fruit is rich in antioxidants: DPPH (EC50 = 0.89 ± 0.03 mg/mL), TEAC (1078 ± 4.9 μM/g), and hemolysis inhibition (IC50 = 358.07 μg/mL), with high phenolic and flavonoid content at 15.92 ± 3.2 mg GAE/g and 4.33 ± 0.3 mg CA/g, respectively. Using analytical chromatography, gallic acid, vanillic acid, chlorogenic acid, ferulic acid, quercetin, and rutin were quantified. The extract also showed α-glucosidase inhibition (IC50 = 3.1 ± 0.17 mg/mL), but no inhibition against α-amylase and lipase (>5 mg/mL), while showing antiproliferative activity against HeLa, HT-29, and MCF-7 cancer cell lines. These results point towards an interesting potential for the fruit of the A. arizonica as chemopreventive and hold potential for elaborating functional foods.

Evaluation of α-glucosidase inhibitor activity and bioactive compounds in purple wheat flour yogurts

Nowadays, the food industry attaches more importance to studies on using purple components in food formulations due to their bioactive properties. This study evaluated the phenolic compound content, antioxidant activity, physicochemical properties, sensory properties, and α-glucosidase activities in yogurt enriched with purple wheat flour. Yogurts were produced using varying concentrations of purple wheat flour labeled as sample A (1.5 %), sample B (3 %), and sample C (4.5 %). It was observed that incorporating purple wheat flour led to a decrease in pH and an increase in L*, a*, and b* values, as well as the acidity and viscosity of the yogurts. Sample C contained the highest phenolic content (37.6 mg GAE/100 g dry matter) on day 14, along with the highest flavonoid content (14.59 mg CE/100 g) on day 21 when than control yogurt. In addition, sample C had the highest α-glucosidase activity (38.35 %) on day 14 and anthocyanin content (13.55 g/100 g) on day 21. As a result, C yogurt can be consumed as a diabetic product with antidiabetic and antihypertensive properties by reaching optimal α-glycosidase inhibition activity. Furthermore, yogurts containing purple wheat flour received higher sensory scores from panelists.

Antiradical effect and phytochemical characterisation of the leaves of Parkia platycephala Benth

The objective of this study was to isolate and identify compounds present in the leaves of Parkia platycephala Benth (Leguminosae-Mimosoideae) and evaluate the antiradical potential of the derived extracts and fractions. Seven compounds were successfully isolated from the ethanolic leaf extract, comprising gallic acid, flavonoids, triterpenoids, and phytosteroids. Structural identification was accomplished through comprehensive spectroscopic data analysis, including NMR and mass spectrometry (ESI-MS), and comparison with existing literature. The antiradical efficacy of both the extract and its fractions was determined in vitro through assays measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and quantifying total phenolics via UV/Vis, revealing notable antioxidant activity in extracts from P. platycephala Benth, especially in the EtOH, EtOAc, and aqueous fractions, attributed to their high phenolic content and prevalence of flavonoids. These findings contribute to a deeper understanding of Parkia’s chemical composition and its potential biological benefits.

Unveiling the therapeutic potential of Haloxylon articulatum extract: a comprehensive study on its phytochemical composition, antioxidant, antifungal, and antibacterial activities

ABSTRACT Haloxylon articulatum, commonly known as the salt tree have diverse medicinal benefits. The phytochemical content, antioxidant, antibacterial, and antifungal properties of the methanolic extract were assessed. The extract demonstrated a high concentration of flavonoids and phenolic components, with a Total Flavonoid Content (TFC) of 186 ± 3 µg QE/mg extract and a Total Phenolic Content (TPC) of 188.1 ± 0.4 µg GAE/mg extract. Bioactive substances with anti-inflammatory and antioxidant qualities, such as quercetin, rutin, chrysin, and caffeic acid, were detected by LC-MS analysis. Through IC50 values of 28.88, 14.13, and 23.03 µg/mL for DPPH, β-carotene, and ABTS tests, respectively, the extract demonstrated considerable antioxidant activity. Antibacterial activity was reported against Escherichia coli and Staphylococcus aureus, with bactericidal effects indicated by MIC and MBC values. The extract also exhibited potent antifungal activity against Thielaviopsis paradoxa and Alternaria sp. These results suggest that H. articulatum extract is a promising source of natural antioxidants and antimicrobial agents with potential therapeutic applications.

Phytochemistry, Nutritional, and Pharmacological Potential of Citrus Limonum

The current research aimed to provide an overview of the phytochemical configuration, nutritional value, and therapeutic uses of Citrous Limonum (lemon). Its fruit contains a variety of phytochemicals including citric acid, polyphenols, terpenes, limonene, flavonoids, vitamin C, sugar, pectin, citric acid, malic acid, flavonoids, carotenoids, terpineol, fellander, camhenium, citrain, calcium oxalates, and mucilages. Vitamin C is abundant in citrous fruits, as well as macronutrients (dietary fibre and simple sugar) and micronutrients (copper, magnesium, phosphorus, calcium, potassium, pantothenic acid, riboflavin, vit B6 niacin thiamin, and folate). Lemon oil is composed of 70% limonene and 20% monoterpenes, along with significant amounts of aldehydes, such as citral, alcohols (linalool), and esters (coumarin). Lemon peel contains high concentrations of flavonoids, glycosides, coumarins, steroids (beta, gamma, sitosterol), dietary fibers, carbs, and volatile oils, all of which are necessary for good health and appropriate development. However, citrus fruits are low in calories, salts, and cholesterol. They find uses in herbal remedies due to their antioxidant, antifungal, anti-cholesterol, anticancer, antiulcer, antidiabetic, antibacterial, and anti-inflammatory characteristics. It is also important to mention that the excessive use of lemon may also be associated with some risks, such as the lowering of sperm count.