High Anthocyanin Content Research Articles

Preserving nutrient content in red cabbage juice powder via foam-mat hybrid microwave drying: Application in fortified functional pancakes.

Red cabbage, a highly nutritious cool-season cruciferous vegetable, is rich in anthocyanins; however, the instability of anthocyanins during processing and storage poses challenges. This study aimed to optimize the foam-mat drying process of red cabbage juice (RCJ) with a high anthocyanin content using a hybrid microwave hot air-drying system (MW-HAD) as a dehydration method compared to conventional techniques (HAD) using response surface methodology (RSM). Additionally, the produced red cabbage juice powder (RCJP) was used to enrich the pancake formulation. The developed model exhibited a high degree of reliability with optimal conditions and was determined for microwave power, temperature, foaming agent carboxymethylcellulose (CMC), and egg white protein (EWP) as 360 W, 60°C, 0.3%, and 1.2%, respectively. Moisture content (%) was decreased from 93.47 to 8.62 at optimum process conditions. In comparison to the control (60°C), foam mat drying with the MW-HAD hybrid system reduced the drying time (DT) by more than 90.9% due to the higher drying rate, while many physicochemical properties, especially total anthocyanin content (TAC), were better preserved. Utilization of RCJP in the production of anthocyanin-rich functional pancakes resulted in enhanced nutritional qualities compared to control pancakes with increased protein (35.07%), total phenolic (75.8%), dietary fiber (82.9%), and anthocyanin content (100%). In conclusion, MW-HAD demonstrates significant potential as a promising drying method to reduce the DT and preserve the physicochemical properties of RCJP. Furthermore, the application of the optimized RCJP in anthocyanin-rich functional pancakes highlights improved nutritional qualities, making a substantial contribution to the advancement of functional foods.

The Ethanolic Extract of Lycium ruthenicum Ameliorates Age-Related Physiological Damage in Mice.

Aging and age-related diseases are important study topics due to their associations with progressive physiological damage to genes, cells, tissues, and the entire organism, which ultimately affects the functional efficiency of organs. Lycium ruthenicum Murr. is a functional food that is known for its high contents of anthocyanins and spermidines, both of which have been demonstrated to have positive effects on anti-aging activity and anti-oxidation. In this study, we used HPLC-MS to analyze the constituents of L. ruthenicum Murr. Extract (LRM) and investigated their potential mechanism for exerting antioxidative effects in D-galactose (D-Gal) aging model mice. LRM (25 mg/kg, 50 mg/kg, and 100 mg/kg) improved cognitive function in D-Gal-treated mice, as shown by reduced escape latencies and increased platform crossings in behavioral tests. We measured the contents of lipid peroxidation (LPO) and malondialdehyde (MDA) and the enzyme activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice serum and brain after 6 weeks of D-Gal treatment. LRM decreased the contents of LPO and MDA and increased the enzyme activities of SOD and GSH-Px, indicating the protection effect of LRM against D-Gal-induced oxidative stress. Additionally, LRM can inhibit oxidative stress in cells by reducing intracellular ROS levels and restoring mitochondrial membrane potential, thereby inhibiting paraquat (PQ)-induced cellular senescence and delaying cell aging. Therefore, LRM has the potential to be a healthcare product for the treatment of age-related diseases.

BRS Vitória grapes with natural surfactant pretreatment: evaluation of mineral composition, physical-chemical characteristics, and sensory characteristics after raisin production

Raisins have been gaining recognition for their nutrient and bioactive compound contents. Yet, pretreatments used for grape drying raise concerns regarding the economic viability and safety of the product. The objective of this proposal was to produce raisins from BRS Vitória grapes pretreated with a natural surfactant to accelerate the drying process. Subsequently, the influence of drying and pretreatment was evaluated for chemical composition (moisture, water activity (Aw), pH, total acidity (TA), reducing sugars (RS), and total sugars (TS)), total phenolic compounds (TPC), total anthocyanins, and qualitative and quantitative mineral composition. Finally, a sensory analysis of the raisins was conducted. By using the pretreatment, drying time decreased by approximately 38.5%. The raisins met the moisture requirements by the Brazilian and international regulations, and the pretreated raisins (PR) had a higher anthocyanin content, a high sugar concentration, acids, phenolic compounds (PC), and minerals (potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), and manganese (Mn)), and were well accepted by consumers, especially in terms of taste and appearance. Interestingly, the pretreatment also provided more brightness to the samples. In conclusion, BRS Vitória grapes yielded raisins with a complex composition, especially those that were subjected to the pretreatment, might be consumed as a healthier snack, adding PC and minerals to the diet.

Optimization and comparison of heat- and ultrasound-assisted extraction methods for anthocyanin recovery from Sicana odorifera fruit epicarp

AbstractThis study is aimed at maximizing the recovery of anthocyanins from Sicana odorifera fruit epicarp by heat- and ultrasound-assisted extraction (HAE and UAE, respectively) and at evaluating the in vitro bioactive properties of the anthocyanin-rich extracts obtained under optimized conditions. The optimization process utilized response surface methodology (RSM) through a central composite rotatory design (CCRD), combining time, ethanol percentage, and temperature or ultrasonic power. Response criteria included extraction yield (determined by gravimetry) and total anthocyanin content (TAC, quantified by HPLC-DAD). Under global optimum conditions, HAE (62 min, 90 °C, 27% ethanol) recovered 281 mg TAC/g extract with a yield of 23% (w/w) extract, while UAE (23 min, 500 W, 40% ethanol) yielded 200 mg TAC/g extract and 26% (w/w) extract, thus confirming the models’ predictive capacity. Furthermore, although HAE has produced extracts with higher anthocyanin concentrations, the cost-benefit associated with the processing conditions of each method must be considered. Both extracts showed antioxidant activity by inhibiting thiobarbituric acid reactive substances and oxidative hemolysis, but did not show anti-inflammatory, cytotoxic, and antimicrobial effects. Overall, this study promotes the upcycling of fruit waste into new ingredients for the food industry and other sectors that use anthocyanins, in line with sustainability and circular economy principles.

Chromosome-level genome assembly and annotation of Zicaitai (Brassica rapa var. purpuraria)

Zicaitai is a seasonal vegetable known for its high anthocyanin content in both stalks and leaves, yet its reference genome has not been published to date. Here, we generated the first chromosome-level genome assembly of Zicaitai using a combination of PacBio long-reads, Illumina short-reads, and Hi-C sequencing techniques. The final genome length is 474.12 Mb with a scaffold N50 length of 43.82 Mb, a BUSCO score of 99.30% and the LAI score of 10.14. Repetitive elements accounted for 60.89% (288.72 Mb) of the genome, and Hi-C data enabled the allocation of 430.87 Mb of genome sequences to ten pseudochromosomes. A total of 42,051 protein-coding genes were successfully predicted using multiple methods, of which 99.74% were functionally annotated. Notably, comparing the genome of Zicaitai with seven other species in the Cruciferae family revealed strong conservation in terms of gene numbers and structures. Overall, the high-quality genome assembly provides a critical resource for studying the genetic basis of important agronomic traits in Zicaitai.

Analyzing Morphology, Metabolomics, and Transcriptomics Offers Invaluable Insights into the Mechanisms of Pigment Accumulation in the Diverse-Colored Labellum Tissues of Alpinia.

Alpinia plants are widely cherished for their vibrant and captivating flowers. The unique feature of this genus lies in their labellum, a specialized floral structure resulting from the fusion of two non-fertile staminodes. However, the intricate process of pigment formation, leading to distinct color patterns in the various labellum segments of Alpinia, remains a subject of limited understanding. In this study, labellum tissues of two Alpinia species, A. zerumbet (yellow-orange flowers) and A. oxyphylla (white-purple flowers), were sampled and analyzed through morphological structure observation, metabolite analysis, and transcriptome analyses. We found that hemispherical/spherical epidermal cells and undulate cell population morphology usually display darker flower colors, while flat epidermal cells and cell populations usually exhibit lighter flower colors. Metabolomic analysis identified a high concentration of anthocyanins, particularly peonidin derivatives, in segments with orange and purple pigments. Additionally, segments with yellow pigments showed significant accumulations of flavones, flavanols, flavanones, and xanthophylls. Furthermore, our investigation into gene expression levels through qRT-PCR revealed notable differences in several genes that participated in anthocyanin and carotenoid biosynthesis among the four pigmented segments. Collectively, these findings offer a comprehensive understanding of pigmentation in Alpinia flowers and serve as a valuable resource for guiding future breeding efforts aimed at developing Alpinia varieties with novel flower colors.

Ethnopharmacology, Nutritional Value, Therapeutic Effects, Phytochemistry, and Toxicology of Salvia hispanica L.: A Review.

The purpose of this review was to emphasize the nutritional value, and pharmacological and phytochemical properties of Salvia hispanica, as well as its toxicological evaluation. Salvia hispanica L. (S. hispanica), also called chia seeds, is an annual herbaceous plant belonging to the family Lamiaceae. It is a species of medicinal and dietary plant used since ancient times by the Maya and Aztecs. Its product is an indehiscent dry fruit that is commonly called a seed. It is utilized for its health benefits and uses in cooking. The study aimed to investigate the pharmacological, phytochemical, and toxicological properties of S. hispanica seeds. The research also attempted to explore and compile all existing knowledge and data on these seeds' nutritional value and medical applications. The current review was conducted using numerous scientific databases, including Science Direct, Scopus, PubMed, Google Scholar, etc. The correct plant name was verified from plantlist.org. The results of this search were interpreted, analyzed, and documented based on the obtained bibliographic information. S. hispanica is a pseudo cereal that is consumed by the world's population because of its preventive, functional, and antioxidant characteristics, attributable to the presence of lipids, dietary fiber, protein, phenolic compounds, vitamins, and minerals. According to research, chia offers hypoglycemic, antimicrobial, anticancer, anti-inflammatory, antioxidant, antihypersensitive, anti-obesity, and cardioprotective properties. Chia consumption has grown because of its favorable benefits on obesity, cardiovascular disease, diabetes, and several forms of cancer. These advantages are mostly due to the high concentration of essential fatty acids, dietary fiber, antioxidants, flavonoids, anthocyanins, vitamins, carotenoids, and minerals found in this seed. Based on the beneficial components, chia seeds have enormous potential in the areas of health, food, animal feed, medicines, and nutraceuticals. Finally, toxicological investigations have indicated the greater doses of chia seed extracts as safe. The current evaluation has focused on the distribution, chemical composition, nutritional value, and principal uses of S. hispanica in order to determine future research requirements and examine its pharmacological applications through clinical studies.

The Content of Anthocyanins in Cowpea (Vigna unguiculata (L.) Walp.) Seeds and Contribution of the MYB Gene Cluster to Their Coloration Pattern.

The intensively pigmented legumes belonging to Phaseolus and Vigna spp. are valued as an essential component of healthy nutrition due to their high content of flavonoids. In this context, we used the accessions of Vigna unguiculata with different colors of seed coats from the N.I. Vavilov All-Russian Institute of Plant Genetic Resources collection as the main object of this research. We applied confocal laser scanning microscopy, biochemical analysis, and wide in silico and molecular genetic analyses to study the main candidate genes for anthocyanin pigmentation within the MYB cluster on chromosome 5. We performed statistical data processing. The anthocyanin content ranged from 2.96 mg/100 g DW in reddish-brown-seeded cowpea accessions to 175.16 mg/100 g DW in black-seeded ones. Laser microscopy showed that the autofluorescence in cowpea seeds was mainly caused by phenolic compounds. The maximum fluorescence was observed in the seed coat, while its dark color, due to the highest level of red fluorescence, pointed to the presence of anthocyanins and anthocyanidins. Genes of the MYB cluster on chromosome 5 demonstrated a high homology and were segregated into a separate clade. However, amplification products were not obtained for all genes because of the truncation of some genes. Statistical analysis showed a clear correlation between the high content of anthocyanins in cowpea seeds and the presence of PCR products with primers Vigun05g0393-300-1.

Essential oil, flavonoids and anthocyanins profiling of some Iranian damask rose (Rosa damascena Mill.) genotypes

Increasing production efficiency is necessary for the industrialization of plant cultivation which includes the type and content of metabolites in medicinal and aromatic plants. In the present study, the metabolic diversity of twenty-four Iranian Rosa damascena genotypes (G1-G24) was studied. The essential oils isolated from fresh flowers and methanolic extracts from dried petal samples were analyzed by chromatographic techniques. In total, forty-three chemical compounds were identified in the essential oil samples. Geraniol (0.2–45.8%), β-citronellol (1.9–22.3%), and nerol (1.4–15.2%) were the major compounds identified in the studied oils especially in G5, G7, and G22, respectively. Twelve flavonoids and four anthocyanins were identified in the extracts. The highest flavonoid content (48.9 mg/g DW) was recorded in G2. Kaempferol 3-O-glucoside (6.1–12.9 mg/g DW), quercetin 3-O-glucoside (0.9–11.4 mg/g DW), and quercetin 7-(6''-galloylglucoside) (0.3–9.2 mg/g DW) were identified as the main flavonoids in the studied genotypes. The highest content of anthocyanin (37.0 mg/g DW) was observed in G1. Cyanidin 3,5-O-diglucoside was found only in G17 and G18, while cyanidin 3-O-sambubioside was unique in G1. β-Citronellol and methyl eugenol had positive (β > 0.5) and negative (β > 0.6) correlations with flavonoids kaempferol 3-O-galactoside 7-O-rhamnoside (K3g7r) and Q3g in the studied samples, respectively; so, this finding can be interestingly used as a phytochemical marker to select the damask rose genotypes producing high-quality essential oil. In conclusion, G21, G2, and G1 can be distinguished and used as adequate genotypes for further commercial cultivation to obtain the essential oil, flavonoids, and anthocyanins, respectively.

Krmiva s povećanim sadržajem antocijanina u hranidbi životinja

The word anthocyanin comes from the Greek words Anthos, meaning flower, and kyanose, meaning blue. Anthocyanins give red, purple, and blue colors to plants. In acidic conditions, they appear as red pigments and in alkaline conditions, they appear as blue pigments. The intensive nature of agricultural (animal) production, with a high density of individuals in animal production facilities, very often contributes to the occurrence of disorders and diseases in animals that threaten this production. A major problem for the animal organism is the effects and consequences of oxidative stress or excessive production of free radicals, which are the main cause of metabolic disorders in cows in the transition period, and antioxidants can prevent or at least mitigate these conditions. Anthocyanins as a source of natural antioxidants play an important role in increasing the antioxidant potential that protects the cell against oxidative damage. Some anthocyanin species may have an enhancing effect on mRNA expression and superoxide dismutase activity. The bioavailability of anthocyanins is relatively low due to poor absorption in the digestive tract. In our region, colored corn, wheat, barley, and sorghum can be used as anthocyanin sources in the domestic animal diet, black soybean, rye, and black and red rice also have high anthocyanin content. Purple corn can accumulate much more anthocyanins than commercial corn varieties.

Physicochemical and bioactive constituents, microbial counts, and color components of spray-dried Syzygium cumini L. pulp powder stored in different packaging materials under two controlled environmental conditions.

Currently, the demand for functional food items that impart health benefits has been rising. Blackberry (Syzygium cumini L.) fruit has high anthocyanin content and other functional attributes. However, this seasonal fruit is highly perishable, and a large proportion of it goes unharvested and wasted worldwide. Spray drying of the fruit pulp can impart improved shelf life, ensuring long-term availability for consumers to exploit its health benefits. The storage quality varies according to the type of packaging material and the storage environment. Therefore, in this study, the shelf life span of the spray-dried Syzygium cumini L. pulp powder (SSCPP) was investigated during 6 months of storage under three types of packaging materials (i.e., polystyrene, metalized polyester, and 4-ply laminates) in a low-temperature environmental (LTE) and at ambient environmental conditions. The physicochemical stability of bioactive principles (TPC and TAC), microbial counts, and color components were analyzed at 0, 2, 4, and 6 months of storage. There was a significant gradual loss of dispersibility and solubility with an increase in flowability, bulk density, and wettability during the entire storage period for all three packaging materials. The TSS, pH, TPC, TAC, and microbial counts decreased in the SSCPP both at ambient and LTE conditions during the study. Among all the packaging materials, the 4-ply laminate was found to be the most appropriate and safe for storage of spray-dried SCPP at LTE conditions.

Microencapsulation of anthocyanins as natural dye extracted from fruits - A systematic review.

Anthocyanins are naturally colored compounds that can be extracted from plants, especially fruits. Their molecules are unstable under normal processing conditions; thus, they must be protected using modern technologies, such as microencapsulation. For this reason, many industries are searching for information from review studies to find the conditions that improve these natural pigments' stability. This systematic review aimed to elucidate different aspects of anthocyanins, such as main extraction and microencapsulation methods, gaps in analytical techniques, and industrial optimization measurements. Initially, 179 scientific articles were retrieved, of which seven clusters were found with 10-36 cross-linked references. Sixteen articles containing 15 different botanical specimens were included in the review, most focusing on the whole fruit, pulp, or subproducts. The extraction and microencapsulation technique resulting in the highest anthocyanin content was sonication with ethanol, temperature below 40°C, and maximum time of 30min, followed by microencapsulation by spray drying with maltodextrin or gum Arabic. Color apps and simulation programs may help verify natural dyes' composition, characteristics, and behavior.

국내 육성 포도 품종 아그데 적포도주 품질 특성

This study aimed to investigate the wine properties of new Korean grape cultivar Ageude. To this end, wine was produced using five grape cultivars including Campbell Early and Muscat Bailey A, which are commonly used for winemaking in Korea and Pinot Meunier and Gamay, which are European wine grape cultivars. We conducted a comprehensive analysis of wine quality properties, color, and phenolic compounds and compared the variations observed among these cultivars. The pH and total acid contents were in the ranges of 3.75-4.57 and 0.39-0.58%, respectively. Specifically, the total acid content of Ageude was 0.47% which was not high, when compared to those of other wines. However, the soluble solid content was 7.85 °Brix, which was higher than those of the other wines. In terms of color, the redness, color intensity, and hue values were all considerably high. Moreover, the total phenolic content and total anthocyanin content of Ageude were notably higher than those of the other cultivars. However, it is worth noting that proanthocyanidin was not detected. Based on these findings, Ageude is an excellent cultivar for producing red wine, owing to its high total anthocyanin content and redness due to the absence of proanthocyanidin, it is more suitable for producing short-term aging wine rather than long-term aging wine.

Comparison of Different Extraction Methods for Bioactive Compounds from Kao-Kum Doi-Saket (Oryza sativa L.)

Kao-Kum Doi-Saket is one type of purple rice known for its high content of anthocyanin and its ability to resist free radicals. Anthocyanin extracts can be utilized in various applications, including food coloring, nutritional supplements, natural medicine, and cosmetics. There are several methods for extracting anthocyanin from Kao-Kum Doi-Saket. This research aims to compare the effects of extraction methods, including Conventional extraction (CE), Pulse electric field extraction (PEF), and Ultrasonic assisted extraction (UAE) on total anthocyanin content and antioxidant activity in Kao-Kum Doi-Saket. It was found that the UAE method resulted in the highest total anthocyanin content of 57.05 ± 4.27 mg/L, which was significantly different from the other two methods (p<0.05). Kao-Kum Doi-Saket extracts from the UAE method also showed the highest DPPH inhibition (88.32 ± 1.83%) compared to the CE and PEF methods. Therefore, these findings suggest that UAE is the most effective method for extracting anthocyanin and antioxidant activity from Kao-Kum Doi-Saket and could be beneficial for developing processed products from Kao-Kum Doi-Saket in the future.

Radish microgreens produced without substrate in a vertical multi-layered growing unit are rich in nutritional metabolites.

Growing microgreens on trays without substrate in a vertical multilayered growing unit offers several advantages over traditional agriculture methods. This study investigated the yield performance and nutritional quality of five selections of radish microgreens grown in sprouting trays, without a substrate using only water, in an indoor multilayer cultivation system using artificial light. Various parameters were measured, including fresh weight, dry matter, chlorophyll, minerals, amino acids, phenolics, flavonoids, anthocyanins, vitamin C, glucosinolates, and antioxidant activity with four different in vitro assays. After ten days, the biomass had increased by 6-10 times, and the dry matter varied from 4.75-7.65%. The highest yield was obtained from 'Asia red', while the lowest was from 'Koregon red'. However, 'Koregon red' and 'Asia red' had the highest dry matter. 'Asia red' was found to have the highest levels of both Chls and vitamin C compared to the other cultivars, while 'Koregon red' exhibited the highest levels of total phenolics and flavonoids. Although variations in the levels of individual glucosinolates were observed, there were no significant differences in the total content of glucosinolates among the five cultivars. 'Asia purple' had the highest anthocyanin content, while 'Asia green 2' had the lowest. The K, Mg, and Na concentrations were significantly highest in 'Asia green 2', and the highest Ca was recorded in 'Asia purple'. Overall, 'Asia purple' and 'Koregon red' were the best cultivars in terms of nutritional quality among the tested radish microgreens. These cultivars exhibited high levels of dry weight, total phenolics, flavonoids, anthocyanins, essential and total amino acids, and antioxidant activities. Moreover, the implementation of this vertical cultivation method for microgreens, which relies solely on water and seeds known for their tall shoots during the sprouting could hold promise as a sustainable approach. This method can effectively be utilized for cultivar screening and fulfilling the nutritional and functional needs of the population while minimizing the environmental impacts associated with traditional agriculture practices.

Influence of freeze-drying parameters on the content of polyphenols and anthocyanins in strawberries

Strawberry berries are a valuable product rich in vitamins, anthocyanins, and polyphenolic compounds that have antioxidant effects on human health. However, due to the deterioration and seasonal availability, the issue of their long-term storage is highly relevant. Freeze-drying, a modern preservation method, preserves their properties during storage. Since the drying process can affect the appearance, nutrient content, and antioxidant activity of berries, the purpose of this study was to investigate the effects of freeze-drying parameters on the content of anthocyanins and polyphenols in Albion variety strawberries. The heating temperatures of the freeze-drying were 35°C, 40°C, 45°C, and 50°C, and the sublimation process times were 18, 20, and 22 hours. Optimal conditions were found: a shelf temperature of 45°C and 18 hours of drying. These conditions preserved both the strawberries' initial qualities and their high anthocyanin content, with only an 8.4% decrease. Similarly, polyphenol content decreased by 3.9%, while the strawberries maintained strong antioxidant activity.

Identification of natural dyes with halochromic properties and their potential use as food spoilage indicators

Purpose The purpose of this research is to identify and investigate some natural dyes with halochromic properties for potential use as food spoilage indicators to reduce waste and curve the negative effects of food borne diseases. Design/methodology/approach Exactly 10 potential dye-yielding plants were selected based on their colour (mostly purple, red, maroon and pink). Solvent extraction was used to extract the dyes and pH differential method was used to determine the concentrations of anthocyanin in the extracted dyes. Different concentrations of hydrochloric acid and sodium hydroxide (0.1 M, 1 M and 2 M) in drops and in excess as acidic and basic solution, respectively, were used to test the halochromicity of the extracted dyes. Methyl red (a synthetic dye) was used as a reference standard/control. The pH of the dyes was recorded before and after addition of both NaOH and HCl solutions. Findings Five out of the 10 dyes extracted (labelled as dye A–E for Ti plant (green Cordyline fruticosa), coleus (Coleus blumei), paper flower (Bougainvillea glabra), painted nettle (Palisandra coleus) and purple heart (Setcresea purpurea), respectively, were found to be halochromic (even at low doses) by changing its colour when exposed to both acidic and basic solutions. While other dyes labelled F–J for red acalypha (Acalypha wilkesiana), golden shower (Cassia fistula), golden dew drop (Duranta repens), wild sage (Lantana camara var Aculeata) and pink oleander (Apocynaceae Nerium oleander), respectively, were either completely insensitive to the solutions in drops, slightly sensitive at high doses or the colour change is insignificant. Although some dyes were found to be more sensitive than others but in most cases, the colour changes in halochromic dyes were more stable in acidic conditions than in basic making it more sensitive to the basic than the acidic solution with the exception of dye A and E (to some extent) which was sensitive to both acidic and basic solution. The anthocyanin contents of dye A–J were found to be between the range of 2.28–10.35 mg/l with dye E having the lowest and dye J with the highest anthocyanin concentration, respectively. The initial pH of all the dyes falls within the range of 4.8–7.3 with most found within the acidic range. Originality/value Halochromic dye research studies are still at the infancy stage in developing world despite the vast available and abundant potential natural halochromic dye-yielding plants. The study explored this area of research and gives an opportunity for the development of smart packaging for pH-sensitive foods using natural dyes as an alternative to conventional synthetic dyes to reduce cost and also curve the negative effect of synthetic dyes as well as food borne diseases.

Enrichment of meat products with natural bioprotectors and antioxidants

In the course of scientific research the chemical composition, physico-chemical and antioxidant properties of local grape and secondary raw materials of winemaking industry were studied, the technology of sausage production with the addition of grape extract of high nutritional value was substantiated and developed. Information on the antioxidant activity of grape raw materials growing in the territory of the Republic of Bashkortostan has been obtained. The presence of biologically active substances with antioxidant and antioxidant properties in the grape extract is a promising factor for the introduction of the extract in food products, including meat products, to enrich them with antioxidants. Analyzing the results of the study, we can conclude that the use of 3% grape squeeze extract will enrich meat products with a sufficient amount of antioxidants of natural plant origin. In the course of research it was found that the extract from grape squeeze has a rich dark blue color due to the high content of anthocyanins, which can affect the organoleptic characteristics of the finished meat product, in connection with that the color characteristics of the minced meat samples were analyzed. An important indicator in our scientific research was the preservation of antioxidants in the process of preparation of the extract and introduction into the minced meat. In this regard, the degree of preservation of calculated and actual total content of antioxidants in different objects was further studied. The technology of obtaining new types of food products - sausages, based on the use of grape extracts has been scientifically substantiated. It has been established that within the framework of the modern theory of positive nutrition it is more reasonable to add grape squeeze extract instead of fatty meat raw materials. This allows to enrich sausage products not only with mineral substances, organic and polyunsaturated fatty acids, vitamins, amino acids, pectin substances, but also to obtain biologically valuable meat products based on secondary grape raw materials.

Proposal of descriptors to study the variability of Vaccinium meridionale Swartz

Agraz, mortiño, or Andean blueberry (Vaccinium meridionale Swartz) is a fruit tree with high potential for national consumption since it is considered a functional food due to its high content of anthocyanins and antioxidants. The morphological description in plants involves both characterization variables that are highly heritable and easily detectable, as well as evaluation variables influenced by the environment and useful for genetic breeding that, together, are called descriptors and allow knowing the variability of the species. The aim of this study was to develop a list of morphological descriptors with the inclusion of variables to characterize and evaluate Vaccinium meridionale Swartz. Observations were made in natural populations of 11 municipalities in Antioquia, three in Boyacá, one in Cundinamarca, two in Nariño, and one in Santander, Colombia, as well as in the ex situ collection established in Rionegro Antioquia, between 2005 to 2011. A descriptor with 38 quantitative, binary, and multi-state variables was developed. Seven of these variables were obtained at the plant level, 10 from the leaf, six from the flower, 14 from the fruit, and one from the seed. The application of the morphological descriptors in in situ and ex situ conditions reported high polymorphism in the qualitative traits and high variation between individuals for the quantitative variables in the collections under study. These variables are of taxonomic and agronomic importance in the knowledge of the species and are essential for producing and marketing the fruit.

Yield Components of Red Yardlong Bean Pod Genotypes (Vigna unguiculata (L.) ssp. sesquipedalis)

One of the legume plants with development potential is the yardlong bean. Because they have a high anthocyanin content, yardlong bean red pods are crucial for maintaining human health. There are multiple red-purple pod lines as a result of the crossing of the two parents purple pod and green pod. The purpose of this study was to assess the yield component of bean lines that were thirteen yards long. The study was carried out in Tembalang, Semarang, Central Java’s Agrotechno Park. Using genotype as the only component, the experiment used a randomized complete block design (RCBD). Agronomic characteristics such as harvesting time, number of pods, pod weight, length, number of seeds per pod, and weight of 1000 seeds were observed. The result showed that one genotype from F7 generation FPT A 3-4-10-1 had superior characters compared to the cultivar check in harvesting time, the number of pods, pod weight, and the number of seeds per pod. The agronomic characters that has been characterized can be may be used as potential donors for future hybridization programs to develop superior yard long bean variety with good yield components.