High Carbon Monoxide Concentrations Research Articles

Isolation and identification of carbon monoxide producing microorganisms from compost

Carbon monoxide (CO) formation has been observed during composting of various fractions of organic waste. It was reported that this production can be biotic, associated with the activity of microorganisms. However, there are no sources considering the microbial communities producing CO production in compost. This preliminary research aimed to isolate and identify microorganisms potentially responsible for the CO production in compost collected from two areas of the biowaste pile: with low (118 ppm) and high CO concentration (785 ppm). Study proved that all isolates were bacterial strains with the majority of rod-shaped Gram-positive bacteria. Both places can be inhabited by the same bacterial strains, e.g. Bacillus licheniformis and Paenibacillus lactis. The most common were Bacillus (B. licheniformis, B. haynesii, B. paralicheniformis, and B. thermolactis). After incubation of isolates in sealed bioreactors for 4 days, the highest CO levels in the headspace were recorded for B. paralicheniformis (>1000 ppm), B. licheniformis (>800 ppm), and G. thermodenitrificans (∼600 ppm). High CO concentrations were accompanied by low O2 (<6%) and high CO2 levels (>8%). It is recommended to analyze the expression of the gene encoding CODH to confirm or exclude the ability of the identified strains to convert CO2 to CO.

Household carbon monoxide (CO) concentrations in a large African city: An unquantified public health burden?

Carbon monoxide (CO) is a poisonous gas produced by incomplete combustion of carbon-based fuels that is linked to mortality and morbidity. Household air pollution from burning fuels on poorly ventilated stoves can lead to high concentrations of CO in homes. There are few datasets available on household concentrations of CO in urban areas of sub-Saharan African countries. CO was measured every minute over 24 h in a sample of homes in Nairobi, Kenya. Data on household characteristics were gathered by questionnaire. Metrics of exposure were summarised and analysis of temporal changes in concentration was performed. Continuous 24-h data were available from 138 homes. The mean (SD), median (IQR) and maximum 24-h CO concentration was 4.9 (6.4), 2.8 (1.0–6.3) and 44 ppm, respectively. 50% of homes had detectable CO concentrations for 847 min (14h07m) or longer during the 24-h period, and 9% of homes would have activated a CO-alarm operating to European specifications. An association between a metric of total CO exposure and self-reported exposure to vapours >15 h per week was identified, however this were not statistically significant after adjustment for the multiple comparisons performed. Mean concentrations were broadly similar in homes from a more affluent area and an informal settlement. A model of typical exposure suggests that cooking is likely to be responsible for approximately 60% of the CO exposure of Nairobi schoolchildren. Household CO concentrations are substantial in Nairobi, Kenya, despite most homes using gas or liquid fuels. Concentrations tend to be highest during the evening, probably associated with periods of cooking. Household air pollution from cooking is the main source of CO exposure of Nairobi schoolchildren. The public health impacts of long-term CO exposure in cities in sub-Saharan Africa may be considerable and should be studied further.

Unveiling the Air Quality Impacts of Municipal Solid Waste Disposal: An Integrative Study of On-Site Measurements and Community Perceptions

This study examines air quality conditions in and around a classroom located in the Sarıçam/Adana region of Türkiye, near the campus of Adana Alparslan Türkeş Science and Technology University and the Sofulu municipal solid waste (MSW) facility. This academic setting was strategically chosen due to its proximity to the waste facility. The study aims to provide a comprehensive view of the environmental and social impacts of solid waste management through a methodological approach that combines quantitative on-site measurements and qualitative survey studies. Findings from measurements and surveys underline the significant and measurable impacts of MSW facilities on the ambient air quality of university residents. The analysis revealed a marked increase in concentrations of key pollutants, including carbon monoxide (CO), hydrogen sulfide (H2S), dust, and methane (CH4). At sampling point N1, H2S levels rose from 0 ppm in July to 13 ppm in November. Methane increased from 0.2% to 2.5% of the Lower Explosive Limit (LEL) at the same point, although it remained within safety limits. Additionally, CO levels showed a 40% increase, and dust concentration levels rose from 0.21 mg/m3 to 2.36 mg/m3 from summer to winter, indicating a seasonal variation likely influenced by the landfill’s operational dynamics, as well as changes in temperature and relative humidity. In particular, the results indicate high concentrations of CO, H2S and dust, which are directly related to air quality degradation. The study also sheds light on the impacts of these waste disposal facilities on the general well-being and health of the university community, particularly on students and staff. In addition to these findings, the study highlights a general lack of awareness in the university community about the impacts of MSW facilities on air quality. This highlights the need for increased education and information dissemination. The results support the development of comprehensive and effective strategies, including technical solutions and public awareness initiatives, to mitigate the impacts of these facilities on residential areas. In conclusion, the impacts of MSW facilities on air quality should be seen as a multidimensional issue that requires a holistic approach addressing environmental, health, social, and educational dimensions.

Spatial modeling of air pollution in Kharkiv city

&#x0D; &#x0D; &#x0D; One of the most pressing problems facing cities, which negatively affects public health. There is a global practice of online monitoring of the content of pollutants in the air of settlements and informing the population about dangerous periods of increased pollution concentrations for certain categories of diseases and the population as a whole. It is also likely that one of the factors of pollution spread is the landscape differentiation of the city’s territory. The purpose of the study presented in this article is to develop statistical and cartographic models of the spatial distribution of air pollutants within the city of Kharkiv and to establish the relationship between the degree of air pollution and types of urban landscapes. Methods. Based on the analysis of the main methodological approaches and tasks of cartographic modeling of the spatial distribution of air pollution, the study substantiates the choice of the modeling method in this study; the features of a large city as an object of cartographic modeling of air pollution are assessed. Results. Based on the results of long-term monitoring by the Kharkiv Regional Center for Hydrometeorology, a statistical analysis of the results of the maximum and average content of pollutants in the atmosphere of Kharkiv was made and the spatial distribution of air pollution in Kharkiv (including (dust, carbon monoxide, nitrogen dioxide, sulfur dioxide, formaldehyde) was modeled, and patterns of pollution distribution were identified according to landscape types. Conclusions. In general, the spatial analysis of pollutant concentrations in the territories of Kharkiv showed that, in terms of maximum concentrations, the southeastern part of Kharkiv is most affected by carbon monoxide, nitrogen dioxide, and formaldehyde. The reason for this is the large area of industrial facilities. The northwestern part of the city has the highest concentrations of dust and sulfur dioxide. There is a lowering of the relief in the floodplain type of landscape and dense development. Intensive traffic and lowered relief (floodplain landscape) cause high average concentrations of dust, carbon monoxide, and sulfur dioxide in the southern part of the city. Nitrogen dioxide concentrations exceed the TLK in the northwest. The reason is the lowering of the relief on the outskirts of the city (floodplain landscape). The central part of the city has a high average concentration of formaldehyde. In general, a pattern of high concentrations of pollutants was found on the floodplains of the Lopan and Kharkiv rivers, with the lowest concentrations confined to watershed types of terrain between the Kharkiv and Lopan rivers and the Udy and Lopan rivers.&#x0D; &#x0D; &#x0D;

Influence of varying carbon oxides concentrations on the selectivity of an electrical sensor utilizing graphene nanoribbons

We have considered a graphene nano ribbon-based two probe-device working on the principle of alteration of current-voltage (I–V) characteristic, upon interactions of its channel material with the adsorbed gas molecules. The analysis of sensing parameters alongside the electron transport mechanism was carried out by using density functional theory (DFT) formulated on the Hohenberg-Kohen paradigm in conjunction with the non-equilibrium green functions (NEGF) method. Electronic transport through similar work reported earlier reveals that the combination of these two techniques coordinates extremely well and yields convincing electronic results. The nanoribbon-based sensing device was employed to evaluate its capability to detect Carbon dioxide (CO2) and Carbon monoxide (CO) molecules. Analysis of the computed result reveals significant modifications in the electronic property of the device upon adsorption of carbon dioxide and carbon monoxide entities on the channel surface. One such significant and dramatic change in the characteristic that we observed is the nature of the channel that transforms from metallic to semi-metallic. Another observed change is the signal amplification capability relative to the pristine device. The signal gets amplified on increasing number of molecular entities from one to six.The transformation in sensor performance is additionally supported by examining variations in multiple parameters, including adsorption energy, charge transfer, differential resistance, and the figure of merit, also known as the peak-to-valley ratio (PVR). The investigation unveiled that the currently designed zigzag graphene nanoribbon (ZGNR) model exhibits high sensitivity to both CO2 and CO gases. This phenomenon is substantiated by the elevated adsorption energy, increased charge transfer, elevated peak-to-valley ratio (PVR), and heightened sensor response. The substantial sensor response, registering at a magnitude of 8.3, underscores the efficacy of the current system in detecting both low and high concentrations of CO and CO2 molecules. The current study emphasizes the notion that understanding the electronic and transport behaviour of zigzag graphene nanoribbons (ZGNR) and analogous materials could offer valuable insights for addressing the threat of environmental pollution.

Carbon Monoxide Pollution and Limited Health Service Access in Third-world Countries

Despite its complexity, carbon monoxide is a key component of indoor and outdoor air pollution. While carbon monoxide arises from natural sources as well as human activities, the most detrimental exposure risks are within the domestic environment. In third-world countries like Guatemala, a notable positive correlation has been observed between severe respiratory diseases and the concentration of carbon monoxide in the air. This correlation can be attributed to these regions' housing conditions and daily lifestyle practices. Additionally, the limited healthcare services accessibility in third-world nations exacerbated the severity of domestic carbon monoxide poisoning cases. It is evident that effective healthcare interventions still need to be fully utilized in third-world countries, and thus, there is a need to move beyond identifying broad strategies and specific policies. Addressing the issues of carbon monoxide exposure and its impact on public health in these regions requires comprehensive approaches that take into account the challenges faced by these countries.&#x0D; To access carbon monoxide concentration in the household, EsayLog USB has been used in this study. The study reviews the amount of carbon monoxide present in certain villages with different conditions and high carbon monoxide concentrations, especially near fires using combustible fuels and houses at high altitudes. The study found that third-world countries' decision-makers should educate communities, build the environment to provide better medical access to the public and implement appropriate regulations to deliver better quality health care to people.

Experimental study on the purification capacity of potted plants on low-concentration carbon monoxide in indoor environment.

Indoor low-concentration carbon monoxide (CO) exposure is widespread worldwide, and potted plants may be a potential means for CO purification. The objective is to evaluate common indoor plants' CO purification and tolerance capacities. Epipremnum aureum (Linden ex André) G.S.Bunting, Chlorophytum comosum (Thunb.) Jacques, Spathiphyllum kochii Engl. & K.Krause, and Sansevieria trifasciata Hort. ex Prain with similar sizes were tested in the glass chamber with initial CO concentrations of 10, 25, 50, 100, 200, and 400 ppm, respectively. (1) The CO purification capacity of the four potted plants is ranked as Epipremnum aureum (Linden ex André) G.S.Bunting > Chlorophytum comosum (Thunb.) Jacques > Spathiphyllum kochii Engl. & K.Krause > Sansevieria trifasciata Hort. ex Prain. Under the purification effect of each plant, the CO concentration in the chamber decreases linearly and significantly (p < 0.05), and within a specific time period, the time-weighted average (TWA) CO concentrations can be reduced to below the corresponding permissible exposure limits specified by some countries and organizations. (2) With the increase of the stomatal number of each plant and the increase in CO concentration, the hourly and cumulative absorbed CO of each plant increase linearly and significantly (p < 0.05). (3) With the increase in CO concentration, the CO purification efficiency of each plant decreases exponentially and significantly (p < 0.05). (4) When the CO concentration was ≤ 50 ppm, all plants could effectively purify CO without damage. When the CO concentration was in the range of 100 ~ 400 ppm, within 2 weeks after the 48-h experiment, the leaf tips of Chlorophytum comosum (Thunb.) Jacques and Epipremnum aureum (Linden ex André) G.S.Bunting were damaged one after another, and the damaged leaf area increased with the increase of CO concentration. However, each plant as a whole still survived. This study demonstrated that different species of potted plants can effectively absorb low concentrations of CO to varying degrees, but higher concentrations of CO will damage the survival of specific species of potted plants.

Short-term exposure to air pollution and emergency room visits for acute respiratory symptoms among adults.

OBJECTIVE: To examine the short-term effect of ambient air pollution on daily acute respiratory emergency room visits among adults.METHODS: A time-series study (June 2017-February 2019) was carried out among adults (≥18 years) visiting a multi-specialty hospital in Delhi. We evaluated the association between the daily levels of particulate matter (PM) <2.5 μm in diameter (PM2.5) and PM <10 μm in diameter (PM10), ozone (O₃), nitrogen dioxide (NO₂), carbon monoxide (CO) and sulphur dioxide and daily count of emergency room (ER) visits for acute respiratory symptoms. Generalised additive model (GAM) was used with the Poisson link function to analyse the associations for 0-1 to 0-7 lag days.RESULTS: A total of 69,400 ER visits were recorded, of which 2,669 were by adults due to acute respiratory symptoms. At 0-7 lag days, an increment of 1 standard deviation in NO₂ and PM2.5 concentration was associated with a percentage increase in acute respiratory ER visits of respectively 53.0% (95% CI 30.84-78.97) and 19.5% (95% CI 4.53-36.65). During 0-7 lag days, a positive trend was observed at higher concentrations of CO (>1.86-3.28 mg/m³), while a negative significant association was observed at low concentrations of CO (<1.171 mg/m³).CONCLUSION: Short-term exposure to ambient NO2 and PM2.5 was associated with acute respiratory emergency visits of adults at lag 0-7 days.

Air pollution and motor vehicle collisions in New York city

Road traffic accidents are a pervasive feature of everyday life, killing 36,500 people, injuring 4.5 million and, overall, generating costs to the American society of $340 billion in 2019. Understanding the underlying factors can improve the design of prevention strategies. We use all road traffic collisions in New York City between 2013 and 2021 (N = 1,269,600) and match each individual collision to the nearest weather and air pollution station. Our study uses highly disaggregated data using an hourly frequency of collisions at a fine spatial level incorporating various air pollutants and weather factors. We employ an instrumental variable approach using temperature inversions to provide exogenous variation in air pollution addressing endogeneity and measurement error concerns. We find that higher concentrations of carbon monoxide (CO) and sulfur dioxide (SO2) increase the number of collisions but leave the severity (persons injured or killed) unaffected. Part of this can be explained by the effect of air pollutants on aggressive behavior: CO (p < .05) and SO2 (p < .01) increase the number of collisions caused by aggressive driving. Interestingly, this channel is only present in male drivers. Our results provide additional evidence that air pollution not only adversely affects health, but also has “non-health” related effects which are costly for the society.

Effects of the river breeze on the transport of gases in Central Amazonia

River breezes occur regularly along the Amazonia river but little is known about their effects on air pollution. During the first week of August 2014, river breeze events were observed on two days on the banks of the Negro River, central Amazonia. The WRF-Chem model was used to analyze this period to understand the formation mechanism and the influence of the river breeze on the transport of pollutants such as ozone (O3) and carbon monoxide (CO). We show that the river breeze occurred due to a reduction of the easterly winds associated with the cooling of the waters of the Negro River. The river breeze was responsible for inhibiting the transport of pollutants to the East, increasing its concentrations while breeze circulation is observed, as well as a re-circulation of these gases on the riverbank where the breeze was associated. The strongest breeze occurred in a forest region which maintained the highest concentrations of CO and O3 on the East bank of the Negro River. In the Urban region, the breeze was less intense and had a shorter duration, however, it was still able to maintain higher concentrations of CO and O3 in the western region of the city of Manaus, which is a predominantly residential region and therefore, the population of this region lives in a more polluted environment during the occurrence of breezes.

Prediction of CO pollutant in Mashhad metropolis, Iran: Using multiple linear regression

AbstractGiven the importance of carbon monoxide (CO) in acute health threats, studies have been conducted in different countries and cities (272 cities in China and 337 cities on a global scale) on the relationship between daily mortality and CO while the spatial analysis of factors affecting CO pollutant in cities, especially in developing countries, has rarely been done. Accordingly, this research has measured the effect of five environmental‐social variables (ESVs) on the spatial distribution of CO pollutants in the metropolis of Mashhad, Iran. CO concentration data were collected in 23 air pollutant monitoring stations in an area of 356 km2 in 2019. Then, the relationship between five variables and CO pollutant were measured using linear and multiple regression by Sentinel 2A and 3 satellite images in ArcGIS and TerrSet software. The results show that the mean CO concentration averages at 1.56 ppm in the whole city. But its range varies between 0.171 and 2.907 ppm, which is a low figure compared with presented standards and does not indicate a critical situation. The results of multiple regression indicate that 42% of the variance in CO concentration is explained by independent variables. Among five independent variables, the beta value of the land surface temperature (LST) and digital elevation model (DEM) variables is negative and positive for the other three variables, including population density, normalised difference vegetation index (NDVI) and normalised difference built‐up index (NDBI). It should be noted that the strongest correlating variable is population density. Prediction of the spatial distribution of CO pollutants shows the division of the city into three areas: (1) the south and southwest slopes of the city with a low concentration; (2) central areas with a medium concentration; and (3) northern and northeastern areas of the city with a high concentration where low‐income groups reside and there are more worn‐out vehicles, motorcycles and industrial workshops. Areas with high CO concentration need more attention from urban managers.

Characteristics of Air Pollutant Distribution and Sources in the East China Sea and the Yellow Sea in Spring Based on Multiple Observation Methods

The composition of marine aerosol is quite complex, and its sources are diverse. Across the East China Sea (ECS) and the Yellow Sea (YS), multi-dimensional analysis of marine aerosols was conducted. The characteristics of carbonaceous aerosols and gaseous pollutants were explored through in situ ship-based observation, MERRA-2 reanalysis datasets and TROPOMI data from Sentinel-5P satellite. Black carbon (BC)’s average concentration is 1.35 ± 0.78 μg/m3, with high-value BC observed during the cruise. Through HYSPLIT trajectory analysis, sources of BC were from the northern Eurasian continent, the Shandong Peninsula, the ECS and Northwest Pacific Ocean (NWPO). The transport of marine sources like ship emissions cannot be ignored. According to the absorption Angstrom exponent (AAE), BC originates from biomass burning (BB) in the shortwave band (~370 nm) and from fossil fuel combustion in the longwave band (~660 nm). Organic carbon (OC), sulfate (SO42−) and BC report higher Angstrom exponent (AE) while dust and sea salt reveal lower AE, which can be utilized to classify the aerosols as being fine- or coarse-mode, respectively. OC has the highest AE (ECS: 1.98, YS: 2.01), indicating that anthropogenic activities could be a significant source. The process of biomass burning aerosol (BBA) mixed with sea salt could contribute to the decline in BBA’s AE. Ship emissions may affect the distribution of tropospheric nitrogen dioxide (NO2) in the ECS, especially during the COVID-19 pandemic. Tropospheric NO2 over the YS has the highest value (up to 12 × 1015 molec/cm2). Stratospheric NO2 has a ladder-like distribution from north to south, and the variation gradient was lower than that in the troposphere. Carbon monoxide (CO) accumulates in the south and east of the ECS and the east of the YS, while the variation over the eastern YS is relatively frequent. Seas near the Korean Peninsula have extremely high CO concentration (up to 1.35 × 1017 molec/cm2).

Carbon Monoxide Production during Bio-Waste Composting under Different Temperature and Aeration Regimes.

Despite the development of biorefinery processes, the possibility of coupling the "conventional" composting process with the production of biochemicals is not taken into account. However, net carbon monoxide (CO) production has been observed during bio-waste composting. So far, O2 concentration and temperature have been identified as the main variables influencing CO formation. This study aimed to investigate CO net production during bio-waste composting under controlled laboratory conditions by varying aeration rates and temperatures. A series of composting processes was carried out in conditions ranging from mesophilic to thermophilic (T = 35, 45, 55, and 65 °C) and an aeration rate of 2.7, 3.4, 4.8, and 7.8 L·h-1. Based on the findings of this study, suggestions for the improvement of CO production throughout the composting process have been developed for the first time. The highest concentrations of CO in each thermal variant was achieved with an O2 deficit (aeration rate 2.7 L·h-1); additionally, CO levels increased with temperature, reaching ~300 ppm at 65 °C. The production of CO in mesophilic and thermophilic conditions draws attention to biological CO formation by microorganisms capable of producing the CODH enzyme. Further research on CO production efficiency in these thermal ranges is necessary with the characterization of the microbial community and analysis of the ability of the identified bacteria to produce the CODH enzyme and convert CO from CO2.

The Enhancement of CO Oxidation Performance and Stability in SO2 and H2S Environment on Pd-Au/FeOX/Al2O3 Catalysts.

Carbon monoxide (CO) is a colourless, odourless, and toxic gas. Long-term exposure to high concentrations of CO causes poisoning and even death; therefore, CO removal is particularly important. Current research has focused on the efficient and rapid removal of CO via low-temperature (ambient) catalytic oxidation. Gold nanoparticles are widely used catalysts for the high-efficiency removal of high concentrations of CO at ambient temperature. However, easy poisoning and inactivation due to the presence of SO2 and H2S affect its activity and practical application. In this study, a bimetallic catalyst, Pd-Au/FeOx/Al2O3, with a Au:Pd ratio of 2:1 (wt%) was formed by adding Pd nanoparticles to a highly active Au/FeOx/Al2O3 catalyst. Its analysis and characterisation proved that it has improved catalytic activity for CO oxidation and excellent stability. A total conversion of 2500 ppm of CO at -30 °C was achieved. Furthermore, at ambient temperature and a volume space velocity of 13,000 h-1, 20,000 ppm CO was fully converted and maintained for 132 min. Density functional theory (DFT) calculations and in situ FTIR analysis revealed that Pd-Au/FeOx/Al2O3 exhibited stronger resistance to SO2 and H2S adsorption than the Au/FeOx/Al2O3 catalyst. This study provides a reference for the practical application of a CO catalyst with high performance and high environmental stability.

Improving carbon monoxide tolerance of Cupriavidus necator H16 through adaptive laboratory evolution.

Background: The toxic gas carbon monoxide (CO) is abundantly present in synthesis gas (syngas) and certain industrial waste gases that can serve as feedstocks for the biological production of industrially significant chemicals and fuels. For efficient bacterial growth to occur, and to increase productivity and titres, a high resistance to the gas is required. The aerobic bacterium Cupriavidus necator H16 can grow on CO2 + H2, although it cannot utilise CO as a source of carbon and energy. This study aimed to increase its CO resistance through adaptive laboratory evolution. Results: To increase the tolerance of C. necator to CO, the organism was continually subcultured in the presence of CO both heterotrophically and autotrophically. Ten individual cultures were evolved heterotrophically with fructose in this manner and eventually displayed a clear growth advantage over the wild type strain. Next-generation sequencing revealed several mutations, including a single point mutation upstream of a cytochrome bd ubiquinol oxidase operon (cydA2B2), which was present in all evolved isolates. When a subset of these mutations was engineered into the parental H16 strain, only the cydA2B2 upstream mutation enabled faster growth in the presence of CO. Expression analysis, mutation, overexpression and complementation suggested that cydA2B2 transcription is upregulated in the evolved isolates, resulting in increased CO tolerance under heterotrophic but not autotrophic conditions. However, through subculturing on a syngas-like mixture with increasing CO concentrations, C. necator could also be evolved to tolerate high CO concentrations under autotrophic conditions. A mutation in the gene for the soluble [NiFe]-hydrogenase subunit hoxH was identified in the evolved isolates. When the resulting amino acid change was engineered into the parental strain, autotrophic CO resistance was conferred. A strain constitutively expressing cydA2B2 and the mutated hoxH gene exhibited high CO tolerance under both heterotrophic and autotrophic conditions. Conclusion: C. necator was evolved to tolerate high concentrations of CO, a phenomenon which was dependent on the terminal respiratory cytochrome bd ubiquinol oxidase when grown heterotrophically and the soluble [NiFe]-hydrogenase when grown autotrophically. A strain exhibiting high tolerance under both conditions was created and presents a promising chassis for syngas-based bioproduction processes.

Rising surface ozone due to anthropogenic activities and its impact on COVID-19 related deaths in Delhi, India

The rapidity and global spread of the COVID-19 pandemic have left several vital questions in the research community requiring coordinated investigation and unique perspectives to explore the relationship between the spread of disease and air quality. Previous studies have focused mainly on the relation of particulate matter concentration with COVID-19-related mortalities. In contrast, surficial ozone has not been given much attention as surface ozone is a primary air pollutant and directly impacts the respiratory system of humans. Hence, we analyzed the relationship between surface ozone pollution and COVID-19-related mortalities. In this study, we have analyzed the variability of various atmospheric pollutants (particulate matter (PM2.5 and PM10), Nitrogen dioxide (NO2), Carbon monoxide (CO), and Ozone) in the National Capital Region (NCR) of India during 2020–2021 using station data and investigated the relationship of the air-quality parameters with the COVID-19 related deaths. In northern parts of India, the concentration of particulate matter (PM2.5 and PM10), Nitrogen dioxide (NO2), Carbon monoxide (CO), and Ozone remain high during the pre- and post-monsoon seasons due to dust loading and crop residue burning (after winter wheat in April & summer rice in November). The westerly wind brings the polluted airmass from western and northwestern parts to Delhi and National Capital Region during April–June and October–November, and meteorological conditions help raise the concentration of these pollutants. Due to long solar hours and high CO concentrations, the ozone concentration is higher from April to June and September. While comparing major air quality parameters with COVID-19-related deaths, we found a good relationship between surface ozone and COVID-19 mortality in Delhi. We also observed a time lag relationship between ozone concentration and mortality in Delhi, so the exposure to Ozone in a large population of Delhi may have augmented the rise of COVID-19-related deaths. The analysis suggested that ozone has a significant relationship with COVID-19 related mortality in Delhi in comparison to other parameters.

DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component

DNA uptake is widespread among microorganisms and considered a strategy for rapid adaptation to new conditions. While both DNA uptake and adaptation are referred to in the context of natural environments, they are often studied in laboratories under defined conditions. For example, a strain of the thermophile Thermoanaerobacter kivui had been adapted to growth on high concentrations of carbon monoxide (CO). Unusual phenotypes of the CO-adapted strain prompted us to examine it more closely, revealing a horizontal gene transfer (HGT) event from another thermophile, Thermoanaerobacter sp. strain X514, being cultured in the same laboratory. The transferred genes conferred on T. kivui the ability to utilize trehalose, a trace component of the yeast-extract added to the media during CO-adaptation. This same HGT event simultaneously deleted a native operon for thiamine biosynthesis, which likely explains why the CO-adapted strain grows poorly without added vitamins. Attempts to replicate this HGT by providing T. kivui with genomic DNA from Thermoanaerobacter sp. strain X514 revealed that it is easily reproducible in the lab. This subtle form of “genome contamination” is difficult to detect, since the genome remains predominantly T. kivui, and no living cells from the original contamination remain. Unexpected HGT between two microorganisms as well as simultaneous adaptation to several conditions may occur often and unrecognized in laboratory environments, requiring caution and careful monitoring of phenotype and genotype of microorganisms that are naturally-competent for DNA uptake.

Assessment of the exergy, emissions, and combustion characteristics of a diesel engine operating on low-glyceride biodiesel blended with diesel fuel

The behavior of biofuels depends on glyceride content, water content, viscosity, density, and other properties that produce different effects on emissions and fuel performance. Therefore, this work performs an experimental evaluation of the performance of low glyceride palm oil biodiesel blended with diesel fuel operating in a compression ignition engine based on an analysis of exergy, emissions, and combustion characteristics. 90 tests were carried out, operating the engine with diesel/biodiesel 90/10% (B10), 80/20% (B20) and 50/50% (B50) fuel blends, under two load conditions at engine speeds of 1500, 1800, 2100, 2400 and 2700 rpm. Among the main results were a higher concentration of carbon monoxide (CO) and carbon dioxide (CO2) was obtained for B10, and lower concentration of nitrogen oxides (NOx) compared with fuels B20 and B50. Moreover, the increase in the fuel blend, allows better burning in premixed phase and reducing burning in the diffuse phase, improving the combustion process of the engine, mainly due to less delay in fuel injection associated with the low content of glycerides in the blend. Concluding that the decrease of glycerides in biodiesel provides adequate performance, a considerable decrease in emissions and homogeneous combustion when blended with conventional diesel.

Carbon Monoxide (CO) and Particulate Matter (PM2.5) Concentration at Central Kalimantan, Indonesia

Peatland forest fires cause high concentrations of gases and particles in the air, especially carbon monoxide (CO) and PM2.5 which are very dangerous for health. Continuous monitoring of gas and particulate concentrations must be carried out during dry and rainy seasons so that the impact of hazards can be immediately identified and actions can be taken to overcome them. Monitoring equipment was placed from south to north at fire-prone locations to see the effect of wind and vegetation on the CO and PM2,5 concentrations. The concentration of Carbon Monoxide (CO) and Particulate Matter (PM2.5) at Central Kalimantan in the dry season is higher than in the rainy season. In the dry season of 2019, CO concentration in four locations was between 7104.1 – 22,127.5 µg/m3, while in 2021, it was the rainy season, so the CO concentration in six locations was lower, ranging from 38.7 – 1371.5 µg/m3. In the dry season of 2019, PM2,5 concentration in four locations was 196.8 – 360.5 µg/m3, while in 2021, at six locations was 5.0 – 40.0 µg/m3. The high concentration of CO and PM2.5 in the dry season needs to be anticipated with a serious handling program to prevent forest and peat fires

Lichen Biodiversity Index (LBI) for the Assessment of Air Quality in an Industrial City in Pahang, Malaysia

Lichen is a well-documented useful biological indicator for monitoring air pollution using various methods such as the biodiversity of lichens. However, the use of lichen biodiversity to estimate air pollution levels in industrial areas, particularly in Malaysia, is still weak, and the determinants of its diversity are unknown. As a result, the purpose of this research is to analyse air pollution in Malaysia’s industrial urban area using the lichen biodiversity index and its determining factor. This research was carried out at Gebeng, Pahang, Malaysia. A total of 14 sample locations were chosen, each with three replication stations. The Lichen Biodiversity Index (LBI) approach was employed in this study to estimate the degree of air pollution in Gebeng. This study also investigated three potential determinants of lichen biodiversity: carbon monoxide (CO) concentration, relative humidity (%), and vehicle motor frequency (per hour). The LBI was plotted and analysed using the Geographical Information System (GIS) programme ArcGIS 10.8.1, and the determining variables were identified using Pearson’s Correlation Coefficient software PAST 4.03. This study discovered a total of 11 lichen species known as metropolitan lichen. The average LBI across Gebeng is 19.5 (moderate alteration). In the industrial region, CO has an inversely significant relation with lichen biodiversity (r = −0.7433), relative humidity has a significant relation with lichen biodiversity (r = 0.8249), and vehicle motors are not significant as a determining factor for lichen biodiversity (r = 0.2068). This study demonstrates that lichen, with its diversity of species in one place, can be utilised to measure and quantify the degree of air pollution in industrial areas. In addition to that, in the context of an industrial city, vehicle motors do not have a significant impact on lichen biodiversity due to the existence of other pollutants sources from industrial activities. Relative humidity is a sign of clean air and humid surroundings, which is good for lichen growth; meanwhile, higher CO concentration will restrict the growth of sensitive-type lichen and will only allow the resistant type of lichen to grow.