High Oleic Acid Content Research Articles

Functionality of Puff Pastry Olive Pomace Oil-Based Margarines and Their Baking Performance.

Designing healthier lipids is a current approach to developing potential functional foods. Olive pomace oil (OPO) has beneficial effects on human health, attributed to its high oleic acid content and unique bioactive compounds. Four puff pastry margarines (PP-M), based on OPO (M1, M2 at 40.8%, and M3, M4 at 30.8%, and cocoa butter at 10%) combined with low molecular weight organogelators, were prepared using two initial cooling rates (M1, M3 at 0.144 °C/min and M2, M4 at 0.380 °C/min) and compared to both commercial puff pastry (PP) butter (CB) and fatty preparation (CFP). Subsequently, six baked PP counterparts were elaborated. Physical-chemical, mechanical properties, and lipid profiles were analyzed in M1-M4 and PP, while thermal properties were determined in M1-M4. Sensory analysis was carried out in PP-M1 and PP-M3 counterparts. Elasticity (G') of M1-M4 samples was between that of controls CB and CFP, although a higher OPO content reduced viscous modulus (G″). The initial cooling rate did not affect the melting behavior of M1-M4. The firmness of PP-M1 was similar to that of PP-CB and PP-CFP, and the better spreadability and plasticity of M1 positively favored PP puffing. In addition, PP-M1 had 36.8% less SFA content than baked PP-CB, and its overall acceptability was similar. For the first time, a new margarine with high OPO content, showing adequate firmness, spreadability, and plasticity, was formulated, which gave rise to PP with appropriate performance and sensory quality and a healthy lipid profile.

Fatty acids and mineral composition of seed oils extracted from different Rosa L. taxa

In the study, the macro and micro elements and fatty acid contents in rose seeds, which are generally evaluated in the waste category, were determined. Among the plants belonging to these taxa, only the registered varieties belonging to four taxa were used, while the others were genotypes. The plant materials of the study include rosehip (R. canina L.), Yildiz variety of rosehip (R. canina L. cv ‘Yildiz’), Syrup rose (R. heckeliana Tratt. subsp. vanheurckiana), Austrian briar (R. foetida Herrm.), lax rootstock [R. caesia Sm. (Syn: R. laxa Retz.)], wild rose [R. montana subsp. woronovii Chaix subsp. woronovii (Lonacz) Ö. Nilsson L.], hybrid landscape roses (R. x hybrida) and Hosap rose [R. pisiformis (Christ) D.] taxa. It was determined that the contents of nitrogen, potassium, phosphorus, magnesium and calcium of the macro elements examined in the seed samples differed statistically from each other. The seeds of different Rosa L. species examined within the scope of the research can be considered as one of the fruit oil sources with its 3.71-10.01% oil content. The fatty acid contents were determined as follows: linoleic acid (ω6) contents in the taxa ranged from 41.63 to 50.11% with an average of 44.88%; oleic acid (ω9) ranged from 20.80 to 30.27% with an average of 24.95%; linolenic acid (ω3) varied between 14.00-28.51% with an average of 19.20%; arachidic acid ranged from 0.75-1.63% and the average was 1.97%; eicosenoic acid ranged between 0.13-0.65% and averaged 0.33%; palmitoleic acid contents ranged from 0.08-0.60; behenic acid varied between 0.08-0.19% with 0.11% average. It was observed that the (ω3/ω6 ratio of the hybrid rose, which is especially used as a landscape rose and whose fruits are not evaluated, had an average value. R. canina ‘Yildiz’ cultivar showed a high (ω3/ω6 ratio, which is important in health terms. The high oleic acid contents found in these taxa are important results.

Stability analyses of sunflower (<em>Helianthus annuus</em> L.) hybrids for oleic acid and yield traits under multi location trials in Pakistan

The development of a hybrid with high oleic acid is an important breeding goal for sunflower. High oleic acid sunflower has better cooking quality due to low oxidation and rancidity. Hence, inbred lines differing for oleic acid content were selected, alongside the development of hybrids where one or both parents exhibited high oleic acid content in edible oil, and then evaluated at various sites (i.e. with comparatively low temperature during sunflower reproductive phase at Sargodha and Faisalabad; while high temperature and low humidity at Bhawalpur and Multan) in Pakistan during spring season. Moreover, autumn season was relatively cool and high humid for sites (Faisalabad and Sargodha, Pakistan). DNA profiling of hybrids differing for oleic acid content using N1-3F/N2-1R confirmed the presence of a high oleic acid allele in the hybrids. Oleic acid content and seed yield components were increased at high temperature and low humidity to a greater extent in spring than in autumn season. Among the hybrids, one (H5) had stable high oleic acid content during the spring season with higher seed yield and kernel to seed percentage than the check cultivars (Hysun-33 and FH-331). Analysis of the combining ability of two locations revealed a relationship between mean oleic acid contents and combining ability, thereby suggesting the effectiveness of selection in developing high oleic acid inbred lines. Newly developed inbred C.112.P was a positive combiner for oleic acid at all sites except Sargodha, while restorer populations such as RH.344, RH.345 and RH.347 were positive male combiners.

Gene action for morpho-biochemical traits of sesame (Sesamum indicum) in different environments

Sesame (Sesamum indicum L.) is a drought tolerant oilseed crop with the highest oleic acid content, which isyet to be explored for its hybrid vigour. Therefore, present study was carried at Sri Karan Narendra AgricultureUniversity, Jobner, Jaipur, Rajasthan during 2019–20 and 2020–21 to study the combining ability for 11 seed yieldand its attributing traits in 10 parents and their 45 crosses in sesame. The effects of general combining ability andspecific combining ability on morpo-biochemical attributes of cultivars were studied utilizing half-diallel cross. Theexperiment was conducted using randomized blocks design (RBD) with 3 replications under different environmentalconditions. Significant variations were detected among parents for all of the recorded attributes and the F1 generation also showed significant differences for all recorded attributes. The GCA (General Combining Ability)/SCA (Specialized Combining Ability) variance ratio was found to be less than one, indicating that non-additive gene action predominated for all tested attributes. The parents RT–346, TKG–22, RT–372 and RT–351 emerged as good general combiners for seed yield per plant and its attributes in all environmental conditions. The crosses RT–346 × RT–351, TKG–22 × RT–372, RT–372 × RT–351 and RT–103 × RT–351 emerged as good specific cross combinations for seed yield and oil content. Incorporating F1 hybrids with a high SCA and parents with a high GCA into multiple crossings could also to be a beneficial strategy for improving seed production and oil content in sesame.

Integrated Analysis of lncRNA–mRNA Regulatory Networks Related to Lipid Metabolism in High-Oleic-Acid Rapeseed

A high oleic acid content is considered an essential characteristic in the breeding of high-quality rapeseed in China. Long-chain non-coding RNA (lncRNA) molecules play an important role in the plant's growth and its response to stress. To better understand the role of lncRNAs in regulating plant reproductive development, we analyzed whole-transcriptome and physiological data to characterize the dynamic changes in lncRNA expression during the four representative times of seed development of high- and low-oleic-acid rapeseed in three regions. We identified 21 and 14 lncRNA and mRNA modules, respectively. These modules were divided into three types related to region, development stages, and material. Next, we analyzed the key modules related to the oil content and the oleic acid, linoleic acid, and linolenic acid contents with physiological data and constructed the key functional network analysis on this basis. Genes related to lipid metabolism, such as 3-ketoacyl-CoA synthase 16 (KCS16) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), were present in the co-expression network, suggesting that the effect of these genes on lipid metabolism might be embodied by the expression of these lncRNAs. Our results provide a fresh insight into region-, development-stage-, and material-biased changes in lncRNA expression in the seeds of Brassica napus. Some of these lncRNAs may participate in the regulatory network of lipid accumulation and metabolism, together with regulated genes. These results may help elucidate the regulatory system of lncRNAs in the lipid metabolism of high-oleic-acid rapeseed seeds.

Prospecting the biofuel potential of new microalgae isolates

The continued search and urgent need for renewable fuel sources have necessitated the exploration of microalgae to identify relevant species for making biofuels. The aim of the study was bioprospecting and screening native microalgae strains from freshwater habitats of the Almaty region, Kazakhstan, to assess the potential for producing biofuel. The studied strains demonstrated simultaneous biomass productivity, lipid productivity, suitable fatty acid composition, and biodiesel properties. The sequence analysis of the ribosomal DNA internal transcribed spacer partial region and ribulose-bisphosphate carboxylase gene (rbcL) led to the identification of five microalgae: Monoraphidium griffithii ZBD-01, Nephrochlamys subsolitaria ZBD-02, Ankistrodesmus falcatus ZBD-03, Parachlorella kessleri ZBD-04, and Desmodesmus pannonicus ZBD-05. P. kessleri had the highest biomass production (1.42 ± 0.08 g L−1 day−1), lipid productivity (29 ± 1.2 g L−1day−1), and C16–C18 fatty acid contents (90%), followed by A. falcatus and M. griffithi. Gas chromatography/mass spectrometry analysis indicated that the dominant fatty acids in these strains were palmitic, stearic, and oleic acids. The calculated biodiesel properties of P. kessleri and A. falcatus based on fatty acid methyl esters (FAME) profiles showed relatively good fuel properties (cetane numbers - 48 and 50; iodine and saponification values - 83.4 and 103.6 g I₂/100 g oil, 260.8 and 199.5 mg KOH g−1), which correlate well with. Our results suggest that P. kessleri and A. falcatus are promising strains for biodiesel production due to their high lipid productivity, fatty acid profile with relatively high content of oleic acid, and suitable biodiesel properties. The isolated native species of microalgae from natural freshwater bodies of the Almaty region present opportunities for further exploitation for the sustainable production of biomass and biodiesel.

Chemometric and Physico-Chemical Characterization of Fruit and Olive Oils from Autochthonous Cultivars Grown in Aragon (Spain)

Olive tree crops and the olive oil market are becoming less and less diverse due to the rise of intensive growth varieties, with the consequent loss of varietal richness provided by oils from minority and autochthonous cultivars. "Royal de Calatayud" and "Negral de Sabiñán" are two local minority cultivars in Aragon (Spain). Fruit parameters such as ripening, fresh weight, and oil yield were evaluated, as well as physico-chemical and chemical composition parameters in olive oil in comparison with "Arbequina", a cultivar widespread in Spain and other countries. Fruits were harvested from October to December in 2017 and 2019. Chemometric analysis revealed significant varietal differences among the three cultivars. In comparison with "Arbequina", higher oil yields were obtained in the two local cultivars. "Royal de Calatayud" has a higher oleic acid content and a greater quantity of phenolic compounds. It thus displays a better nutritional profile than "Arbequina". This preliminary study shows that "Royal de Calatayud" could be regarded as an excellent alternative to the "Arbequina" cultivar in the parameters analyzed.

White Lupin (Lupinus albus L.), an Alternative Legume for Animal Feeding in the Mediterranean Area

Interest in white lupine has increased in recent years in animal nutrition due to its balanced protein and lipid content, phytochemical compounds, and low alkaloid content. Agronomic traits, nutrients, and antioxidant phenols of six white lupin genotypes grown in a Mediterranean area were explored. Genotypes significantly differed in growth stages and life cycle length (from 172 to 204 days after sowing), plant height (from 36.1 to 97.2 cm), seed yield (from 1.02 to 3.50 Mg ha−1), and yield components. Seed yield was positively correlated with the number of seeds per pod and the thousand-seed weight. Across the average of genotypes, a high protein content (397 g kg−1), a low fiber content (133 g kg−1), and oil content (116 g kg−1) with a high oleic acid (453–509 g kg−1) and low erucic acid content (8–17 g kg−1) were found. The n3/n6 ratio varied from 1:1 to 1:4. Ecotype G showed the highest TPC, DPPH•, and ABTS•+ and Multitalia showed the highest content of Apigenin 1 and 2 derivatives. The lowest alkaloid content was recorded in Volos, Luxor, and Lublanc. Overall, this species can be considered a suitable feed crop and a valuable ingredient in animal nutrition due to its overall nutritional profile. At present, only Volos, Luxor, and Lublanc can be suggested in animal nutrition due to the low alkaloid content. Nonetheless, interesting agronomic and quality traits in Multitalia and the two ecotypes suggest room for breeding to reduce their antinutritional factors.

Genetic Diversity of Korean Black Soybean (Glycine max L.) Germplasms with Green Cotyledons Based on Seed Composition Traits

The demand for black soybeans (Glycine max (L.) Merr.) with green cotyledons is increasing because of their health benefits. Therefore, it is important to understand the genetic diversity of black soybean germplasms and to develop a new soybean cultivar. This study aimed to evaluate genetic diversity among 469 black soybean germplasms with green cotyledons based on seed composition traits. Twenty seed composition traits were analyzed to conduct correlation analysis, principal component analysis (PCA), and cluster analysis, which indicated that black soybean germplasms were divided into four clusters. Black soybean germplasms in cluster 1 had higher crude fat, lutein, chlorophyll a, chlorophyll b, and total chlorophyll contents, but lower cyanidin-3-glucoside content than those in clusters 2 and 3. However, germplasms in clusters 2 and 3 had the highest cyanidin-3-glucoside content. Moreover, germplasms in cluster 1 had significantly higher palmitic acid content than those in clusters 2 and 3. Germplasms in clusters 2 and 3 had relatively high α-linolenic acid content. Germplasms in cluster 4 had the highest oleic acid content. This study highlights the genetic diversity of black soybean germplasms with different seed composition traits, and the results of this study can be beneficial for soybean breeding programs, enabling them to develop new black soybean cultivars with green cotyledons and improved seed composition traits.

Macauba (Acrocomia aculeata): promising source of nutrients and association with health benefits, a review

Macauba is widely distributed in Brazilian territory. In addition to being used in the biodiesel production and animal food, it is also rich in bioactive compounds. Therefore, the objective of this review is to present the nutritional composition, the main bioactive compounds, and the potential health benefits of macauba. Macauba has high content of carotenoids, tocopherols, proteins, phenolic compounds, oleic acid, and fiber. Due the presence of bioactive compounds, macauba have the potential to promote health and can be used to prevent or treatment chronic non-communicable disease. Furthermore, this fruit and their by-products can be used in the development of new food and pharmaceutical products, promoting benefit family farming and contribute to the health benefits.

Carcass trait, meat yield and quality characteristics of recently-synthesized Woori Heukdon and commercial LYD pigs under identical rearing condition.

For decades, LYD ([Landrace×Yorkshire] ♀×Duroc ♂) pigs are the most commonly-used commercial breed for meat production in Korea. Recently, due to the increasing demand for premium pork, the National Institute of Animal Science (Korea) has synthesized a novel pig breed named Woori Heukdon (WHD). This study aimed at comparing the carcass traits, meat yield and quality characteristics between the LYD and WHD pigs under identical rearing condition. The WHD and LYD pigs (n = 15 each) were reared under identical conditions and fed the same commercial diet until reaching recommended market weight (100 to 120 kg). After slaughter, the carcasses were evaluated for traits and meat yield, and the meat quality was assessed on shoulder butt and belly cuts. Although no significant differences (p>0.05) occurred in slaughter weight between two pig types, WHD had a lower meat yield (by about 6 kg corresponding to approximately 7%) compared to the LYD pigs (p<0.05). The WHD had a higher fat content (by 4.26% and 13.52% in the shoulder butt and belly, respectively) compared to those of LYD pigs (p<0.05). The WHD meat showed a lower cooking loss and higher a* (redness) value (p<0.05). The WHD belly had a significantly (p<0.05) higher oleic acid content and concentrations of nonanal, octanal and decanal associated with fatty odor while, the LYD meat had a higher number of pyrazines associated with roasty odor. Regarding sensory quality, higher flavor, juiciness, and acceptability higher scores were given for the WHD meat than for the LYD meat (p<0.05). Under identical rearing conditions the WHD exhibited a better meat quality and sensory properties. However, the use of this diet resulted in a high fat level that may be associated with high trimming loss for the WHD.

Impact of vegetable fat on the sensory and physicochemical quality characteristics of chevon sausage

Goat fat forms unstable emulsions in making sausages and pork fat may be added however, Muslims have restrictions on ingesting pork. This study attempts to investigate the effect of plant-based fat (corn oil, palm oil, and margarine) on the sensory and physicochemical properties of chevon sausage. The moisture content, crude fat, saturated fat, ash, crude protein, and cooking loss of the three different sausage formulations were evaluated. Fifty consumers evaluated the sensory acceptability in terms of color, aroma, taste, texture, and overall acceptability. The most preferred sausage was subjected to microbiological and porcine DNA test. Saturated fat levels in corn oil, palm oil, and margarine, were 3.68%, 6.76%, and 4.41%, respectively. Among the treatments, the chevon sausage prepared with corn oil exhibited the least cooking loss. Although all treatments were rated between like moderately and like very much, the taste of chevon sausage prepared with palm oil was judged to be less appealing than the sausage with corn oil. With regards to proximate composition and sensory acceptability in terms of color and aroma, the results for the three treatments were comparable. The microbiological results showed that the chevon sausage with corn oil is safe to eat within 54 days if stored at -18 °C and the sausage is free from porcine DNA. The fatty acid composition of chevon sausage formulated with pre-emulsified corn oil is characterized by its high content of palmitic acid (16.21%), stearic acid (8.51%), oleic acid (28.31%), and linoleic acid (43.55%).

Масличность рапса : ботаническая природа , биохимические особенности и пищевой потенциал

The annual rapeseed (Brassica napus L.), a member of the Brassicaceae family, has long been used as a source of technical oil. The development of low-erucic varieties (canola, less than 5% erucic acid) by Canadian breeders in the 1970s made rapeseed a valuable and promising oilseed crop. In the Russian Federation, rapeseed oil, extracted from the seeds of the mature pods of the plant, currently ranks third after sunflower and soybean oil in terms of production volume, surpassing them in a number of biochemical parameters: optimal ratio of ω‑6: ω‑3 fatty acids (1:3–2:1), high content of oleic acid (up to 79.57%), tocopherols (45–75 mg%), carotenoids (0.30–0.57 mg%) and sterols (0.5–1.0%). The high nutritional potential of rapeseed is explained by the availabilityof winter and spring forms, domestic and foreign varieties and hybrids, successful breeding for increased oil content and resistance to diseases, good yields in many natural zones of Russia, profitability of 100–150%, sometimes up to 400% and more, despite the need for strict adherence to cultivation technology, etc. In the fatty oil industry, oil yield refers to the content of crude fat and accompanying fat-like substances that pass from the seeds into the ether extract along with the fat. The cells of oil rapeseeds contain structures, that accumulate free lipids stored by the plant for use by the growing seedling: oleosomes and possibly fat inclusions in the cytoplasm, and plastoglobules in the plastids. The formation of neutral fats is a universal mechanism for “switching off” excess primary synthesis products from plant metabolism. The qualitative fatty acid composition of seed oils of different plant species (in contrast to oils extracted from the nonseed parts of the fleshy oil fruits) is quite similar; the amount of fatty acids and the fat-soluble components extracted with them (antioxidants, vitamins, etc.) is variable. Knowledge of the botanical nature and biochemical characteristics of the oil content of rapeseed will make it possible to obtain maximum nutritional benefits through breeding, agronomic and technological methods.

Study of the Apoptotic Impacts of Hazelnut Oil on the Colorectal Cancer Cell Line.

Hazelnut oil has a unique structure with a high oleic acid content, tocopherol, tocotrienols, and other bioactive compounds, such as phytosterols. These biochemical compounds have been widely studied because of their potential health properties. Understanding the process of apoptosis is the basis of new therapies contributing to cancer cells' death. Recently, the potential role of the evolutionary-reserved bcl-2 protein family in tumor progression and prognosis of some malignancies has been addressed in several studies. The present study is aimed at evaluating the effect of apoptotic properties of hazelnut oil on colorectal cancer cells through the major members of this family (bax and bcl-2). MTT assay, apoptotic cell staining (using Annexin V and propidium iodide), flow cytometry, and real-time PCR were used to evaluate the toxicity, percentage of apoptotic cells, and bax and bcl-2 genes' expression after exposing HT29 cells to hazelnut oil. After hazelnut treatment, significant decreases in cell viability, and the gene expression of bax and bcl-2 were observed compared to the control group (P < 0.05). In addition, the total percentage of apoptotic cells after hazelnut oil treatment showed a significant increase in comparison with the negative control group (P < 0.05). Hazelnut oil appears to cause the death of cancerous cells through an apoptotic mechanism.

Oxidative stability of milk and expression of oxidative stress response, lipogenic-related, and IGF-1 genes in early-lactation cows supplemented with peanut and sunflower oils

Context Feeding dairy cows with plant oils rich in unsaturated fatty acids is a recommended strategy for increasing the proportion of polyunsaturated fatty acids in milk for human consumption. Aims We evaluated the effects of supplementation with peanut oil (high oleic acid content) and sunflower oil (high linoleic acid content) on the fatty acid profile and oxidative stability of milk and the expression of oxidative stress response, lipogenic-related and insulin-like growth factor 1 (IGF-1) genes in pasture-fed Jersey cows in early lactation. Methods Twenty-four cows were distributed in a randomised block design. Treatments were four dietary supplements of concentrate alone, or concentrate plus oil (59.6 g/kg dry matter): peanut oil, sunflower oil, or a 1:1 (v/v) mixture of peanut and sunflower oils. Analyses were performed at 30 and 60 days in milk. Key results Both peanut oil and mixed oil diets increased the hypocholesterolemic/hypercholesterolemic ratio (P &lt; 0.001) and health-promoting index (P &lt; 0.001) of milk. The sunflower oil diet increased the thrombogenicity index (P = 0.005) and conjugated diene production (P &lt; 0.001). mRNA abundance of fatty acid synthase (P = 0.003), acetyl-CoA carboxylase alpha (P = 0.001), and stearoyl-CoA desaturase (P &lt; 0.001) decreased with the sunflower oil diet. mRNA abundance of superoxide dismutase decreased (P = 0.01) with sunflower oil and mixed oil diets. The control diet tended (P = 0.08) to reduce IGF-1 mRNA abundance. Conclusions and implications Supplementation with peanut oil increases the health-promoting index of milk, whereas supplementation with sunflower oil increases susceptibility to lipid peroxidation. Supplementation with sunflower oil reduces the expression of lipogenic and antioxidant enzyme genes, and supplementation with unsaturated fats may reduce IGF-1 gene expression in early-lactation cows. The relatively small sample size means that more studies are needed.

Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.

High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil as a new energy source. Hence, the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in soybean. In this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis, a 662 bp fragment was acquired by specific PCR amplification, and the pMD18T cloning vector was linked by the use of a seamless cloning technique. Bioinformatics analysis of the signal peptide prediction, subcellular localization, protein hydrophobicity, transmembrane region analysis, a phosphorylation site, protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried out. The plasmid of the gene editing vector is pBK041. The overexpression vector was transformed from pCAMBIA3301 as the base vector, and overexpression vector were designed. Positive plants were obtained by genetic transformation by the pollen tube channel method. Fluorescence quantitative PCR was performed on the T2 generation plants to detect the relative expression levels in different tissues. Southern Blot was used to detect the presence of hybridization signal. Screening genes BAR, 35S, and NOS in plants were identified by conventional PCR. 10 seeds with high and low oleic acid content were chosen for quantitative PCR identification, and finally, the concentration and morphology of soybean fatty acids were identified by near-far infrared spectroscopy. On 10 seeds with an upper and lower oleic acid content, a quantitative fluorescence analysis was done. In Southern blot hybridization, the SAM22 gene was integrated into the recipient soybean plant in hands of a sole copy. Fluorescence quantitative PCR appeared that the average relative expression of the SAM22 gene in roots, stems, leaves, and seeds was 1.70, 1.67, 3.83, and 4.41, respectively. Positive expression seeds had a 4.77% increase in oleic acid content. The level of oleic acid in the altered seeds was reduced by 4.13% when compared to CK, and it was discovered that the GmSAM22 gene could be a regulatory and secondary gene that promotes the conversion of stearic acid to oleic acid in soybean. There has not been a discussion of gene cloning or functional verification. The cloning and genetic transformation of the soybean SAM22 gene can effectively increase the content of oleic acid, which lays a foundation for the study of soybean with high oleic acid.

CRISPR/Cas9-mediated targeted mutagenesis of FAD2-1 gene for oleic acids composition in sunflower

Sunflower (Helianthus annuus L.) is the most widespread plant used for production of oil among all oilseeds cultivated in Turkey in terms of agricultural area and production. Sunflower with high oleic acid content is always desirable because of benefits on health and industrial use. The enzyme FAD2-1 catalyzes the conversion of oleic acid to linoleic acid in sunflower. The fatty acid composition can be enhanced by gene editing of FAD2-1 gene by CRISPR/Cas9 technique which has been applied recently as a new breeding technique to improve productivity and enhance sustainability in agriculture. Hence, CRISPR/Cas9 genome editing systems were utilized to knockout the FAD2-1 gene in order to increase production of oleic acid content. For this purpose, two low oleic sunflower genotypes were transformed with two sgRNA that target FAD2-1 gene. sgRNA expression cassettes were assembled into the binary vector by Golden Gate assembly in a single reaction which is followed by A. tumefaciens-mediated transformation and in vitro germination. Putatively transformed shoots were selected with A. tumefaciens-mediated the optimized kanamycin concentration (100 mg/L) in the medium. The challenges of transformation of sunflower were summarized and possible solutions were proposed. This study indicated that sunflower still can be modified by CRISPR/Cas9 genome editing system to develop high oleic sunflower.

CRISPR/Cas9-Mediated Mutagenesis of GmFAD2-1A and/or GmFAD2-1B to Create High-Oleic-Acid Soybean

Soybean (Glycine max (L.) Merr.) oil is an important source of vegetable oil for supporting the human diet. However, the high level of polyunsaturated fatty acids in natural soybean oil renders the oil unstable and thus susceptible to the development of unpalatable flavors and trans fatty acids. Therefore, reducing the content of polyunsaturated fatty acids and increasing the content of monounsaturated fatty acids is a longstanding and important target for soybean breeding. However, soybean varieties with a high oleic acid content are rare in soybean germplasm resources, which introduces substantial difficulties in the cultivation of high-oleic-acid soybeans. In this study, CRISPR/Cas9-mediated gene-editing technology was used to create targeted knockout of the soybean fatty acid desaturase encoding genes GmFAD2-1A and GmFAD2-1B that contribute to the formation of polyunsaturated fatty acids. We obtained fad2-1a, fad2-1b, and fad2-1a/fad2-1b homozygous mutants using two sgRNAs. We found that the oleic acid content increased from 11% to 40-50% in the fad2-1a and fad2-1b mutants and to 85% in the fad2-1a/fad2-1b mutants. We also generated transgene-free double mutants that conferred higher oleic acid, and the fad2-1a/fad2-1b mutant had no adverse phenotyping compared with the wild type. Our study provided new materials for the selection and breeding of high-oleic-acid soybean varieties.

Formulation Of Liquid Soap Based on Sea Grass (Eucheuma Cottonii) Used Olive Oil as An Oil Phase

The potential of Indonesian marine biota products is very large to be processed into commercial commodities. One of them is seaweed Eucheuma cottonii which contains phenolic profiles, vitamin A, vitamin C, vitamin E and various minerals that play a role in healing and rejuvenating the skin. This liquid soap product contains the main ingredients of Eucheuma cottonii seaweed pulp and an olive oil soap base. Olive oil has a high content of oleic acid which is often used as a good antioxidant and moisturizing agent in the field of cosmetics. Objective to obtain a liquid bath soap formulation with the active ingredient of Eucheuma cottonii seaweed pulp using optimization of olive oil as an oil phase. Testing seaweed includes moisture content, ash content, content fat, protein content and heavy metal contamination. The method of making Eucheuma cottonii seaweed pulp is by blanching seaweed, soaking in 1:10 deonize water for 12 hours, draining, then homogenizing it with deonize water 1:2 for 3 minutes. The seaweed pulp is then formulated into liquid bath soap with the optimization of the best olive oil formula. The results showed that seaweed had 22.27% water content, 29.55% ash content, 0.53% fat content, 5.89% protein content and Pb &lt; 0.25; Hg&lt;0.1. Seaweed slurry obtained a clear mass and expands and can be used as a gelling agent. The soap formula obtained specific gravity 1.0894-1.1206 g / ml, pH value at range 6.26-6.43, viscosity in the number 420 cp - 2,800 cp and all preparations are physically and chemically stable during storage. Conclusion Eucheuma cottonii seaweed porridge can be formulated into soap and meets the requirements of liquid soap

Quality Change in Camellia Oil during Intermittent Frying.

Camellia oil with a high oleic acid content is widely used for frying. To comprehensively describe the quality change in camellia oil during frying, the changes in composition, deterioration indicators, and volatile profiles were investigated. The results showed that tocopherols mainly degraded in the early stage of frying, followed by unsaturated fatty acids (UFA). This caused the carbonyl value and total polar compounds level to significantly increase. Moreover, frying promoted the accumulation of volatile compounds in terms of type and abundance, especially aldehydes, which are related to the degradation of UFA. Principal component analysis showed that the frying of camellia oil was divided into three stages. First, the camellia oil with a heating time of 2.5-7.5 h showed excellent quality, where tocopherol played a major role in preventing the loss of UFA and was in the degradation acceleration stage. Subsequently, as tocopherol entered the degradation deceleration stage, the quality of camellia oil heated for 10.0-15.0 h presented a transition from good to deteriorated. Finally, tocopherol entered the degradation stagnation stage, and the quality of camellia oil heated for 17.5-25.0 h gradually deteriorated, accompanied by a high level of volatile compounds and deterioration indicators. Overall, this work comprehensively determined the deterioration of camellia oil during intermittent frying and offered valuable insights for its quality evaluation.