High Cd Accumulation Research Articles

Root radial apoplastic transport contributes to shoot cadmium accumulation in a high cadmium-accumulating rice line

Radial transport of cadmium (Cd) in roots governs the amount of Cd loaded into xylem vessels, where Cd ions are translocated upward into shoots, while the mechanism of differential Cd radial transport between the high Cd-accumulating rice line Lu527–8 and the normal rice line Lu527–4 remains ambiguous. A higher Cd distribution in cross sections and root apoplast and higher bypass flow of Cd were found in Lu527–8, explaining a greater Cd translocation through the apoplastic pathway. The lower relative area of the epidermis and the constant relative area of the cortex in Lu527–8 opened-up root radial transport for Cd. Deposition of apoplastic barriers (Casparian strips and suberin lamellae) was stimulated by Cd, which effectively prevented Cd from entering the stele through the apoplastic pathway. In Lu527–8, apoplastic barriers were further from the root apex with lower expression of genes responsible for biosynthesis of Casparian strips and suberin lamellae, enhancing radial transport of Cd. Our data revealed that the higher radial apoplastic transport of Cd played an integral role in Cd translocation, contributed to a better understanding of the mechanism involved in high Cd accumulation in Lu527–8 and helped achieve the practical application of phytoextraction.

Enrichment of cadmium and selenium in soil-crop system and associated probabilistic health risks in black shale areas.

Selenium (Se) is the essential component of selenoenzymes and contributes to antioxidant defenses. The capability of Se to antagonize the toxicity of heavy metals makes it an essential trace element for human and plant health. Soils derived from black shales are naturally enriched with Se; however, these soils often contain high geological cadmium (Cd), due to the weathering of black shales rich in Cd and Se. Cadmium, as a known Group I carcinogen, could induce damage to various organs. This therefore poses a major challenge for safe cultivation of Se-rich land resources. In this study, a total of 247 paired soil-crop samples were collected from a typical farmland derived from black shales. The concentrations of Cd and Se in the samples were analyzed by inductively coupled plasma mass spectroscopy and atomic fluorescence spectrometry. Monte Carlo simulation was applied to evaluate potential health risks associated with Cd exposure. Cadmium was the critical pollutant in the study area, with the average value of 1.53 mg/kg. Moreover, both children and adults living in the area had a significant non-carcinogenic health risk. Additional health risk assessments revealed that diet was the main contributor for both children and adults among the four pathways (diet > soil ingestion > soil dermal adsorption > soil inhalation). Furthermore, our results revealed that leguminous vegetables and maize were ideal for this site due to their high Se and low Cd accumulation abilities. These findings provide support for adjusting planting structure by variety screening to mitigate the health risk induced by Cd.

Comparative transcriptome analysis reveals key genes and coordinated mechanisms in two rice cultivars differing in cadmium accumulation

Although Cd accumulation varies among rice varieties is recognized, the underlying mechanisms are not well clarified. In this study, comparative transcriptome analysis were performed by hydroponic culture system with two rice varieties, Y1540 (high Cd accumulator) and Y15 (low Cd accumulator) under 20 μM Cd stress. Results revealed 17,320 differentially expressed genes (DEGs) in roots of Y15 (7,655 upregulated and 9,665 downregulated) and 17,386 DEGs in roots of Y1540 (8,823 upregulated and 8,563 downregulated) expose to 20 μM Cd stress. Gene ontology (GO) analysis enriched 24 and 26 terms in Y15 and Y1540 respectively, including 23 common terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment showed 27 and 28 significant pathways in Y15 and Y1540 respectively, with 19 common pathways. Different responses to Cd stress between cultivars were not only reflected in differently enriched GO terms and KEGG pathways but also in different DEGs of 23 common GO terms and significant sequences represented by p-values of 19 common KEGG pathways. Both cultivars resist Cd through common processes with different weights; hence glutathione metabolism, mineral absorption, biosynthesis of secondary metabolites, and degradation of aromatic compounds could be playing a more important role in Y1540, whereas ribosome biogenesis in eukaryotes, mismatch repair, aminoacyl-tRNA biosynthesis, and the cell cycle maybe playing a more important role in Y15. Weighted gene co-expression network analysis (WGCNA) showed that five and three modules were clustered in Y15 and Y1540, respectively, with yellow and brown modules in Y15 and brown modules in Y1540 being significantly related to Cd stress. Further analysis showed that most of hub genes in Y15 were related to signal transduction or transcription factors, while most of hub genes in Y1540 were related to binding, metabolic, and secondary metabolic processes, which demonstrated their different response patterns at transcriptomic level to Cd stress.

Pleiotropic melatonin-mediated responses on growth and cadmium phytoextraction of Brassica napus: A bioecological trial for enhancing phytoremediation of soil cadmium

Melatonin (MT) has recently gained significant scientific interest, though its mechanism of action in enhancing plant vigor, cadmium (Cd) tolerance, and Cd phytoremediation processes are poorly understood. Therefore, here we investigated the beneficial role of MT in improving growth and Cd remediation potential of rapeseed (Brassica napus). Plants, with or without MT (200 µM L−1), were subjected to Cd stress (30 mg kg1). Without MT, higher Cd accumulation (up to 99%) negatively affected plant growth and developmental feature as well as altered expression of several key genes (DEGs) involved in different molecular pathways of B. napus. As compared to only Cd-stressed counterparts, MT-treated plants exhibited better physiological performance as indicated by improved leaf photosynthetic and gaseous exchange processes (3–48%) followed by plant growth (up to 50%), fresh plant biomass (up to 45%), dry plant biomass (up to 32%), and growth tolerance indices (up to 50%) under Cd exposure. MT application enhanced Cd tolerance and phytoremediation capacity of B. napus by augmenting (1) Cd accumulation in plant tissues and its translocation to above-ground parts (by up to 45.0%), (2) Cd distribution in the leaf cell wall (by up to 42%), and (3) Cd detoxification by elevating phytochelatins (by up to 8%) and metallothioneins (by upto 14%) biosynthesis, in comparison to Cd-treated plants. MT played a protective role in stabilizing hydrogen peroxide and malondialdehyde levels in the tissue of the Cd-treated plants by enhancing the content of osmolytes (proline and total soluble protein) and activities of antioxidant enzymes (SOD, CAT, APX and GR). Transcriptomic analysis revealed that MT regulated 1809 differentially expressed genes (828 up and 981 down) together with 297 commonly expressed DEGs (CK vs Cd and Cd vs CdMT groups) involved in plant-pathogen interaction pathway, protein processing in the endoplasmic reticulum pathway, mitogen-activated protein kinase signaling pathway, and plant hormone signal transduction pathway which ultimately promoted plant growth and Cd remediation potential in the Cd-stressed plants. These results provide insights into the unexplored pleiotropic beneficial action of MT in enhancing in the growth and Cd phytoextraction potential of B. napus, paving the way for developing Cd-tolerant oilseed crops with higher remediation capacity as a bioecological trial for enhancing phytoremediation of hazardous toxic metals in the environment.

Potential of Ornamental Trees to Remediate Trace Metal Contaminated Soils for Environmental Safety and Urban Green Space Development

Heavy metals are notoriously toxic pollutants which can potentially harm living beings and are serious health hazards. The aim of the present study was to assess the levels of cadmium (Cd) and nickel (Ni) throughout the year in the urban areas of the densely populated industrial city of Faisalabad in eight commonly raised ornamental tree species with phytoremediation potential. High levels of Cd and Ni were recorded in all study areas, with spatio-temporal heterogeneity. Heavy metal uptake varied among plant species with Cd and Ni in soil ranging between 6.78–8.57 mgkg−1 and 46.31–55.85 mg kg−1 respectively. Plant species accumulated 6.73–8.98 mg kg−1 Cd and 26.42–52.50 mg kg−1 Ni with Conocarpus erectus, Dalbergia sissoo and Bismarckia nobilis showing higher accumulation potential than others. Dalbergia sissoo accumulated the highest levels of Ni and was shown to a good bio-indicator for this metal. The highest accumulation of Cd was recorded in Conocarpus erectus (9 mg kg−1), followed by Dalbergia sissoo (8.2 mg kg−1) and Bismarckia nobilis (8.1 mg kg−1) while the leaves of Azadirachta indica retained the lowest (6.3 mg kg−1) Cd levels. The highest levels of metals were accumulated by all species during the summer season while the second highest were observed in the autumn season. The study revealed that ornamental species can help in minimizing heavy metal pollution as well as providing green space in urban settings for maintaining a clean and healthy environment.

Assessment of the capability of cadmium accumulation and translocation among 31 willows: four patterns of willow biomass variation response to cadmium.

Cadmium (Cd) pollution threatens food security and the environment. Willow species (Salix, Salicaceae) exhibit a remarkable potential to restore Cd-polluted sites due to their high biomass production and high Cd accumulation capacities. This study examined the Cd accumulation and tolerance in 31 genotypes of shrub willow in hydroponic conditions at varying Cd levels (0μM Cd, 5μM Cd, and 20μM Cd). The root, stem, and leaf biomass of 31 shrub willow genotypes showed significant differences to Cd exposure. Among 31 willow genotypes, four patterns of biomass variation response to Cd were identified: insensitive to Cd; growth inhibition due to excessive Cd supply (high Cd inhibition); low Cd causing inhibited growth, whereas high Cd leading to increased biomass (U-shape); and growth increment with excessive Cd exposure (high Cd induction). The genotypes belonging to the "insensitive to Cd" and/or "high Cd induction" were candidates for the utilization of phytoremediation. Based on the analysis of Cd accumulation of 31 shrub willow genotypes at high and low Cd levels, genotypes 2372, 51-3, and 1052 obtained from a cross between S. albertii and S. argyracea grew well and accumulated relatively more Cd levels than other genotypes. In addition, for Cd-treated seedlings, root Cd accumulation was positively correlated with shoot Cd accumulation and total Cd uptake, demonstrating that Cd accumulation in roots could serve as a biomarker for evaluating the Cd extraction capacity of willows, especially in hydroponics screening. The results of this study screened out willow genotypes with high Cd uptake and translocation capacities, which will provide valuable approaches for restoring Cd-contaminated soils with willows.

Overexpression of LSU1 and LSU2 confers cadmium tolerance by manipulating sulfur metabolism in Arabidopsis

Phytoremediation using plants is an environmentally friendly and cost-effective strategy for removing cadmium (Cd) from soil. Plants used for phytoremediation must have a high Cd accumulation capacity and strong Cd tolerance. Therefore, understanding the molecular mechanism of Cd tolerance and accumulation in plants is of great interest. In response to Cd exposure, plants produce various thio-rich compounds, such as glutathione, phytochelatins, and metallothioneins, which play important roles in Cd immobilization, sequestration, and detoxification. Therefore, sulfur (S) metabolism is crucial for Cd tolerance and accumulation. In this study, we report that the overexpression of low-S responsive genes, LSU1 and LSU2, confers Cd tolerance in Arabidopsis. First, LSU1 and LSU2 promoted S assimilation under Cd stress. Second, LSU1 and LSU2 inhibited the biosynthesis and promoted the degradation of aliphatic glucosinolates, which could limit the consumption and enhance the release of S, thus, facilitating the production of the S-rich metabolites, glutathione, phytochelatins, and metallothioneins. We further demonstrated that the Cd tolerance mediated by LSU1 and LSU2 was dependent on the myrosinases BGLU28 and BGLU30, which catalyze the degradation of aliphatic glucosinolates. In addition, the overexpression of LSU1 and LSU2 improved Cd accumulation, which has great potential for the phytoremediation of Cd-contaminated soil.

Cadmium Absorption in Various Genotypes of Rice under Cadmium Stress.

Cadmium (Cd) is a kind of heavy metal. Cadmium pollution in paddy fields will accumulate a large amount of cadmium in rice, which will affect the growth and development of rice. In addition, long-term consumption of rice contaminated with Cd can harm human health. In this study, four rice varieties with high Cd accumulation (S4699, TLY619, JHY1586, QLY155) and four varieties with low Cd accumulation (YY4949, CYJ-7, G8YXSM, MXZ-2) were screened through field experiments for two consecutive years (2021 and 2022) and differences in antioxidant enzyme systems and expression of genes in their organs were analyzed. The total Cd content showed as follows: indica rice > japonica rice, high-Cd-accumulation variety > low-Cd-accumulation variety, and the total Cd content of each organ of rice showed root > stem > leaf > grain. The results of the antioxidant enzyme system showed that the contents of malondialdehyde (MAD), reduced glutathione (GSH), oxidized glutathione (GSSH), and peroxidase (POD) were positively correlated with the total Cd content in rice, and superoxide dismutase (SOD) showed the opposite performance in the leaves. There was no correlation between catalase (CAT) and Cd content, but CAT content decreased in leaves and grains and increased in roots and stems with increasing fertility. Based on this study, RT-qPCR was used to further validate the expression of Cd-uptake-related genes in different rice varieties. It was found that high expression of OsHMA3, OsCCX2, OsNRAMP5, and OsHMA9 genes promoted Cd uptake and translocation in rice, especially in rice varieties with high Cd accumulation. The high expression of OslRT1, OsPCR1, and OsMTP1 genes hindered Cd uptake by rice plants, which was especially evident in low-accumulating Cd rice varieties. These results provide an important theoretical reference and scientific basis for our in-depth study and understanding of the mechanism of cadmium stress tolerance in rice.

Silicon Calcium Fertilizer Application and Foliar Spraying with Silicon Fertilizer Decreases Cadmium Uptake and Translocation in Rice Grown in Polluted Soil

Rice cultivated in Cd-polluted acidic paddy soil poses important health risks in China. Decreasing Cd accumulation in rice is important for food safety and human health. Early rice cultivar ZY-819 and late rice cultivar XWX-13 with low Cd-accumulation potentials, and early rice cultivar LY-996 and late rice cultivar YZX with high Cd-accumulation potentials, were grown in mildly polluted double-cropping paddy fields (Cd content 0.3–0.6 mg kg−1). The effects of adding biochar (10 t ha−2), lime (1500 kg ha−2), and silicon–calcium fertilizer (SC; 2250 kg ha−2) and foliar spraying with silicon fertilizer solution (Si; 1500 g ha−2) on Cd uptake and transport in rice, were assessed in plot experiments. The soil amendments and foliar spraying decreased the Cd content of brown rice from the high Cd-accumulation potential cultivars. The soil amendments decreased the Cd content of LY-996 and YZX brown rice by 25.24–32.40% and 32.99–44.16%, respectively, and SC decreased the Cd content most. Foliar spraying with Si decreased the Cd content of LY-996 and YZC brown rice by 23.79% and 26.40%, respectively. When soil amendments and foliar spraying were combined, the Cd content of brown rice was decreased most by the SC–Si treatment. Compared with the control, the SC–Si treatment decreased the Cd content of LY-996, ZY-819, YZX, and XWX-13 brown rice by 45.63%, 35.67%, 52.79%, and 32.03%, respectively. Soil amendments can effectively decrease Cd uptake by rice roots and Cd migration from roots to shoots. Compared with the control, the soil amendments increased the soil pH and decreased Cd availability. The strongest effects were for the lime and SC treatments. Foliar spraying with Si can effectively decrease Cd translocation through stems and leaves to brown rice. Applying SC fertilizer and foliar spraying with Si is the best method for decreasing the Cd content of rice grown in mildly Cd-polluted paddy fields.

Mobility, bioaccumulation in plants, and risk assessment of metals in soils

Heavy metal contamination of soils is one of the main factors contributing to soil quality decline and loss of biodiversity, which is also associated with plant contamination, as metals accumulate in the surface layer of soils and then enter the trophic chain. The aims of the study were to assess the mobility and bioavailability of metals in soils to plants, and to estimate the ecological and health risks associated with heavy metal content in soils. 320 topsoil and 206 plant samples were collected. Fractional analysis showed that for most of the samples, there was no or low risk associated with the mobility of Cr, Pb, Cu, Ni, Zn, and low and medium for Cd. High and very high metal release risk was only shown for Cd (28 % of samples), and Zn and Pb (2 % of samples). The bioaccumulation factor found moderate levels of accumulation for Cd, Zn, Cu, Ni. High accumulation of Cd and Zn was found in 38 % and 15 % of plant samples. Alivibrio fischeri proved to be a more sensitive indicator of soil ecotoxicity compared to Sinapis alba. In the 81 % of the soil samples found a low probability of adverse effects on ecological receptors associated with exposure to soilborne metals. In the case of human health risk, no harmful health effects were observed due to accidental ingestion of metal-containing soils in the study area. In assessing metal risks, the choice of indicators is crucial. Moreover, the properties of soils have a significant impact on the mobility of metals and their bioaccumulation by plants. This means that the more varied the choice of indicators, the more comprehensive, reliable and close to reality the risk assessment of heavy metals in soils will be.

Biotransfer of heavy metals along the soil-plant-edible insect-human food chain in Africa

Although mining is Zambia's major economic activity, it is implicated in environmental contamination, particularly with heavy metals. This study investigated the accumulation and transfer of heavy metals along the soil-plant-edible insect-human food chain. Our results revealed the presence of eight heavy metals (Arsenic, Cadmium, Chromium, Copper, Iron, Nickel, Lead, and Zinc) with a 28–60 % increase in soil concentrations at the proximity of the mining facilities. There was a higher accumulation of Cd, Cu, Ni, Fe, Pb, and Zn than As and Cr in plant species near the mine. Among the insect species studied, C. forda accumulated nickel significantly higher (70–81 %), I. obscura had higher cadmium (2–84 %) and lead (10–79 %), while I. rubra and M. falciger accumulated higher iron (41–96 %) and zinc (1–67 %), respectively, than other insect species. The quantity of I. obscura consumed (248 g person−1 day−1) was significantly higher (9–37 %) than other insect species. It was noted that the consumption of insects increased the daily intake of heavy metals, enhanced the target hazard quotient, and increased the associated health risks by up to 9 folds compared to the WHO permissible limits meaning that the daily intake of metals consumed depends on the daily quantity of insects consumed. Our findings suggest that the accumulation of heavy metals along the soil-plant-edible insect-human food chain could pose severe human and environmental health risks along the mining gradients. The potential consequences of heavy metal mobility in the consumer trophic levels and the ecotoxicological consequences are particularly concerning. Furthermore, physiological and biological studies are needed to investigate the abovementioned effects.

Putrescine-functionalized carbon quantum dot (put-CQD) nanoparticle: A promising stress-protecting agent against cadmium stress in grapevine (Vitis vinifera cv. Sultana)

Due to their sessile nature, plant cannot escape from stress factors in their growing environment, in either biotic or abiotic nature. Amid the abiotic stress factors; high levels of soil cadmium (Cd) impose heavy metal stress on plants, resulting in critical injuries and reduced agronomic performance. In order to buffer the adverse effects of Cd stress, novel nanoparticles (NP) have been applied and notable improvements have been reported. According to the literature, the protective roles of polyamines (e.g., Putrescine; Put) and carbon quantum dots (CQD) have been reported with respect to the plant productivity under either stress or non-stress conditions. Those reports led us to hypothesize that the conjugation of Put and CQD (Put-CQD NPs) might lead to further augmented performance of plants under stress and non-stress conditions. In this regard, we successfully synthesized a novel nanomaterial Put-CQD NPs. In this respect, Put (50 mg L−1), CQD (50 mg L−1) and Put-CQD NPs (25 and 50 mg L−1) were sprayed in ‘Sultana’ grapevines under Cd stress (10 mg kg−1). As expected, upon stress, Cd content in leaf and root tissues increased by 103.40% and 65.15%, respectively (p < 0.05). The high uptake and accumulation of Cd in plant tissues were manifested in significant alterations of physiological and biochemical attributes of the plant. Concerning stress markers, Cd stress caused increases in content of induced MDA, H2O2, and proline as well as electrolyte leakage rate. As expected, Cd stress caused critical reductions in fresh and dry leaf weight by 21.31% and 42.34%, respectively (p < 0.05). On the other hand, both Put-CQD NPs increased fresh and dry leaf weigh up to approximately 30%. The Cd-mediated disturbances in photosynthetic pigments and chlorophyll fluorescence were buffered with Put-CQD NPs. Of the defence system, enzymatic (SOD, APX, GP) as well as anthocyanin and phenolics were induced by both Cd stress and Put-CQD NPs (p < 0.05). On the other hand, Cd stress reduced content of polyamines (putrescine (Put), spermine (Spm) and spermidine (Spd) by 39.28%, 53.36%, and 39.26%, respectively (p < 0.05). However, the reduction levels were buffered by the treatments. Considering the effectiveness of both NP concentrations, the lower dose (25 mg L−1) could be considered as an optimal concentration. To our knowledge, this is the first report of its kind as a potential agent to reduce the adverse effects of Cd stress in grapevines.

Pleurotus pulmonarius Strain: Arsenic(III)/Cadmium(II) Accumulation, Tolerance, and Simulation Application in Environmental Remediation.

The arsenic (As, III) and cadmium (Cd, II) accumulation and tolerance traits of a new strain Pleurotus pulmonarius MT were evaluated, and the utilization of the strain for repairing contaminated liquid and soil was explored. The hypha cultivated in potato dextrose agar (PDA) exhibited medium or high Cd accumulation (0 to 320 mg/L), medium Cd tolerance (maximum tolerated concentration, MTC ≥ 640 mg/L), medium As accumulation (0 to 80 mg/L), and high As tolerance (MTC > 1280 mg/L). The hypha has application potential in processes related to the removal of Cd and As in aqueous pollutants at concentrations of 80 mg/L Cd and 20 mg/L As. The trends obtained for the fruiting bodies of P. pulmonarius MT seemed to deviate from those of the hypha of this strain. The results show that the fruiting bodies featured medium As accumulation (0 to 40 mg/kg), medium As tolerance (MTC > 160 mg/kg), medium Cd accumulation (0 to 10 mg/kg), and high Cd tolerance (MTC > 1280 mg/kg). The fruiting bodies of P. pulmonarius MT were utilized in processes related to the recovery of Cd and As in substrates, that is, 12% contaminated soil mixed with 50 mg/kg Cd and 200 mg/kg As; thus, the hypha and fruiting bodies of P. pulmonarius MT can be used for the decontamination of water and soil containing As(III) and Cd(II).

Genome and Transcriptome Identification of a Rice Germplasm with High Cadmium Uptake and Translocation.

The safe production of food on Cd-polluted land is an urgent problem to be solved in South China. Phytoremediation or cultivation of rice varieties with low Cd are the main strategies to solve this problem. Therefore, it is very important to clarify the regulatory mechanism of Cd accumulation in rice. Here, we identified a rice variety with an unknown genetic background, YSD, with high Cd accumulation in its roots and shoots. The Cd content in the grains and stalks were 4.1 and 2.8 times that of a commonly used japonica rice variety, ZH11, respectively. The Cd accumulation in the shoots and roots of YSD at the seedling stage was higher than that of ZH11, depending on sampling time, and the long-distance transport of Cd in the xylem sap was high. Subcellular component analysis showed that the shoots, the cell wall, organelles, and soluble fractions of YSD, showed higher Cd accumulation than ZH11, while in the roots, only the cell wall pectin showed higher Cd accumulation. Genome-wide resequencing revealed mutations in 22 genes involved in cell wall modification, synthesis, and metabolic pathways. Transcriptome analysis in Cd-treated plants showed that the expression of pectin methylesterase genes was up-regulated and the expression of pectin methylesterase inhibitor genes was down-regulated in YSD roots, but there were no significant changes in the genes related to Cd uptake, translocation, or vacuole sequestration. The yield and tiller number per plant did not differ significantly between YSD and ZH11, but the dry weight and plant height of YSD were significantly higher than that of ZH11. YSD provides an excellent germplasm for the exploration of Cd accumulation genes, and the cell wall modification genes with sequence- and expression-level variations provide potential targets for phytoremediation.

Evaluation of Cd phytoremediation by Portulaca oleracea irrigated by contaminated water

Background: Worldwide, more than 10 million regions with an area of more than 20 million hectares are considered contaminated soils. The expansion in industrial activities and urbanization has led to accumulation of high concentration of cadmium (Cd) in soil and water resources, which is a serious danger for environment and human health. Phytoremediation is a technique recommended for the removal of heavy metals from contaminated soils, sediments, and waters. This study aimed to investigate the Portulaca oleracea in the removal of Cd from contaminated soil. Methods: To investigate the effect of P. oleracea phytoremediation on the removal of Cd, in 5 pots, 3 kg of soil was poured. And in each pot, 10 seeds were pursued. The pots were irrigated twice a week for 8 weeks with concentrations of 25, 50, 75, and 100 mg/L Cd. After 8 weeks, the amount of Cd of roots, shoots, and leaves was measured by atomic absorption spectrometry. Results: The results showed that Cd accumulation in purslane plants was significant (P=0.008) and the highest Cd accumulation was in leaves and shoots, and increasing Cd concentration prevented plant growth and led to lower growth at higher concentrations. Conclusion: According to the results, P. oleracea can be used for the phytoremediation of Cd.

Cadmium accumulation responses in Hylotelephium spectabile: The role of photosynthetic characteristics under different nitrogen, moisture, and light conditions

The influence of environmental factors on Cd accumulation by Hylotelephium spectabile and its physiological mechanisms are unclear. A field trial was conducted to investigate the effects of nitrogen, soil moisture, and light regulation on plant growth, Cd absorption and translocation, and the photosynthetic characteristics of two H. spectabile populations (LN with high Cd accumulation capacity and HB1 with relatively low Cd accumulation capacity). The results showed that Cd accumulation in LN was 59.6% higher than that in HB1 which may partly be explained by the inherent high transpiration rate of LN, especially at the terminal stage. In addition, the photosynthetic rate of LN responded more positively to nitrogen than HB1, which further amplified its advantages on plant growth and Cd accumulation. Moderate drought significantly stimulated root growth of LN, indicating that LN possesses stronger resistance to drought. Shade inhibited Cd distribution, rather than directly affecting Cd concentrations in H. spectabile. The combined stress of shade and drought had a synergistic effect on Cd translocation in H. spectabile. Moreover, LN achieved 17.3%∼444.5% higher transpiration levels than HB1 under environmental stress, which ensured a more efficient Cd transport capacity of LN. Therefore, the investigation of photosynthetic characteristics further revealed the physiological mechanism by which LN accumulated Cd superior to HB1 under environmental stress and responded more positively to nitrogen nutrition.

Mixing tannery effluent had fertilizing effect on growth, nutrient accumulation, and photosynthetic capacity of some cucurbitaceous vegetables: A little help from foe.

Tannery effluent contains a number of organic and inorganic elements as pollutants which reduce plant growth. To overcome shortage of water, use of diluted industrial wastewater such as tannery effluent can be a viable strategy for improving crop growth and yield. A pot experiment was conducted to determine the effects of tannery effluent and its various dilutions on physiological and biochemical characteristics of five cucurbitaceous vegetables. Tannery effluent was applied 0, 25, 50, 75 and 100% to 3-week-old plants of five cucurbitaceous vegetables (Cucurbita maxima, Luffa cylindrica, Citrullus vulgaris, Cucumis melo, and Praecitrullus fistulosus) for 4weeks. Tannery effluent reduced the growth of all five cucrbitaceous vegetables. Diluted tannery effluent (25%) improved the growth of Cucurbita maxima, Citrullus vulgaris, and Cucumis melo. Moderately diluted (50%) did not affect the growth of Citrullus vulgaris and Cucumis melo. Toxic effects of tannery effluent were associated with high accumulation of heavy metals Cr, Cd, Mn, and Fe in leaves and roots. High accumulation of heavy metals in leaves reduced the accumulation of nutrients in leaves (N, P, K) and reduced photosynthetic pigments and photosynthetic rate. Changes in photosynthetic rates of all vegetable species due to tannery effluent were not associated with stomatal limitations (stomatal conductance, transpiration rate, internal CO2). Toxic effects of tannery effluent on plants also include changes in N-metabolism (amino acid and protein). However, extent of these adverse effects of tannery effluent on vegetables was species specific. It is suggested that Cucurbita maxima can be grown by supplying 25% tannery effluent, whereas Citrullus vulgaris and Cucumis melo can be grown with moderately diluted (50%) tannery effluent.

Soil applied silicon and manganese combined with foliar application of 5-aminolevulinic acid mediate photosynthetic recovery in Cd-stressed Salvia miltiorrhiza by regulating Cd-transporter genes.

Salvia miltiorrhiza is an important medicinal plant that experiences significant growth and biomass losses when cultivated on cadmium (Cd) contaminated soils. High Cd accumulation in plant tissues also increases the risk of metal entry into the food chain. In this study, we proposed that Cd accumulation in S. miltiorrhiza can be restricted through plant growth regulators and nutrient management. Therefore, S. miltiorrhiza seedlings were transplanted into mixed nutrient soil for two weeks, then treated with 30 mg kg-1 CdCl2, 200 mg kg-1 Na2SiO3·9H2O, and 100 mg kg-1 MnSO4, and simultaneously sprayed with 10 mg L-1 ALA on the leaves one week later. This study showed that elevated Cd accumulation significantly reduced plant growth and biomass. This growth inhibition damaged photosynthetic machinery and impaired carbon assimilation. In contrast, 5-aminolevulinic acid (ALA) significantly promoted the biomass of S. miltiorrhiza, and the dry weight of plants treated with ALA combined with manganese (Mn)/silicon (Si) increased by 42% and 55% as compared with Cd+Mn and Cd+Si treatments. Exogenously applied ALA and Si/Mn significantly activated antioxidant enzymes and promoted the growth recovery of S. miltiorrhiza. Further, exogenous ALA also reduced the Cd concentration in S. miltiorrhiza, especially when combined with Si. Compared with the Cd+Si treatment, the Cd+Si+ALA treatment reduced the Cd concentration in roots and leaves by 59% and 60%, respectively. Gene expression analysis suggested that ALA and Si significantly up-regulated genes associated with Cd transport. Other genes related to heavy metal tolerance mechanisms are also regulated to cope with heavy metal stress. These results indicated that the combined action of ALA and Si/Mn could reduce Cd-toxicity by increasing chlorophyll content and changing oxidative stress and can also affect Cd accumulation by regulating gene expression.

Molecular insights into lignin biosynthesis on cadmium tolerance: Morphology, transcriptome and proteome profiling in Salix matsudana

Soil pollution caused by cadmium (Cd) is a serious concern. Phytoremediation is a popular technology in the remediation of Cd-contaminated soil. Salix matsudana var. matsudana f. umbraculifera Rehd. has been characterized as a high Cd-accumulating and tolerant willow (HCW). Here, transcriptome and proteome profiling, along with morphology analyses were performed to explore molecular cross-talk involved in Cd tolerance. Our results showed that 73%− 83% of the Cd in roots accumulated in the cell walls and root xylem cell walls were significantly thickened. From transcriptome and proteome analysis, a total of 153 up-regulated differentially-expressed genes and 655 up-regulated differentially-expressed proteins were found in common between two comparison groups (1 d and 4 d vs. respective control). Furthermore, phenylpropanoid biosynthesis was identified as a key pathway in response to Cd stress. In this pathway, lignin biosynthesis genes or proteins were significantly up-regulated, and lignin content increased significantly in roots under Cd stress. Two Cd-induced genes cinnamoyl-CoA reductase 1 (SmCCR1) and cinnamyl alcohol dehydrogenase 7 (SmCAD7) from HCW increased the lignin content and enhanced Cd tolerance in transgenic poplar calli. These results lay the foundation for further clarifying the molecular mechanisms of Cd tolerance in woody plants.

Mining candidate genes for rice cadmium accumulation in the shoot through a genome-wide association study and transcriptomic analysis.

High cadmium (Cd) accumulation in rice is a serious threat to human health. The genetic mechanism of Cd accumulation in rice is highly complicated. To identify the low Cd accumulation in rice germplasm, investigate the genetic mechanism underlying Cd accumulation, and mine the elite genes of significant importance for rice breeding of low Cd accumulation varieties, we performed a genome-wide association study (GWAS) for rice Cd concentration in the shoot. The rice accessions were 315 diverse indica rice accessions selected from the 1568 rice accessions with 700,000 SNPs. Within the high rate of linkage disequilibrium (LD) decay, eight QTLs related to rice Cd accumulation were identified. Transcriptomic analysis showed there were 799 differentially expressed genes (DEGs) in the root and 857 DEGs in the shoot, which are probably considered to be the cause of the significant difference in Cd accumulation between high and low Cd accumulation varieties. In qCd11-1, we detected a crucial candidate gene, LOC_Os11g11050, which encodes an initiation factor, expressed differently in the root between the high and low Cd accumulation varieties. Furthermore, under Cd treatment, the expression levels of LOC_Os11g11050 significantly decreased in both the high and low Cd accumulation varieties. Sequence comparison and qRT-PCR revealed that there were indel sequences and base substitutions in the promoter region of LOC_Os11g11050 correlated with the LOC_Os11g11050 expression level, as well as the phenotype of Cd concentration differences in shoot between the high and low Cd accumulation accessions. LOC_Os11g11050 might play important roles in Cd accumulation. The results of our study provide valuable resources for low Cd accumulation in indica varieties and the candidate functional gene, as well as molecular mechanisms for Cd accumulation in indica rice. The genetic architecture underlying Cd accumulation in indica can be used for further applying the low Cd gene existing in indica for decreasing Cd accumulation in rice.