Candidiasis is the most incessant fungal disease affecting human population worldwide. In this research work, we explored the characteristics contributing to pathogenicity of Candida infections, from serious bloodstream conditions like candidemia. Candida spp. were isolated from the blood of 120 suspected patients and assessed their susceptibility to antifungal treatments via MIC measurements. Enzyme activities (phospholipase, proteinase, hemolysin, and esterase) were examined alongside biofilm formation, with surface analysis conducted via SEM. Statistical analysis, facilitated by GraphPad Prism 8.4.2, identified correlations among data points. Results reveal that non-albicans spp. like Candida auris heightened resistance to antifungal treatments compared to Candida albicans. Candida tropicalis exhibited no phospholipase activity but shows activity to proteinase and esterase. Conversely, C. albicans show a case of elevated hemolysin activity. Non-albicans Candida i.e. C. tropicalis and Candida krusei were isolated as high biofilm formers in comparison to C. albicans. Statistical analysis yielded a highly significant p-value (< 0.001), affirming the robustness of our results. In conclusion, non-albicans species exhibit a broader spectrum of resistance mechanisms compared to C. albicans in pathogenicity. This investigation sheds light on diverse virulence strategies employed by different Candida species, informing future treatment approaches.