Abstract: Although acne is a common bacterium on human skin, it acts as a primary pathogen that causes skin irritation and inflammation. Because of the resistance of acne-causing bacteria to antibiotics, research on novel antibacterial chemicals is crucial. This present research work aims to formulate and evaluate herbal anti-acne face serum containing extract of Neem, Aloevera, Hibiscus, and Nutmeg essential oil. The pinene component of nutmeg oils was principally responsible for their antibacterial properties. various vitamins, like vitamins B12, A, C, and E, as well as enzymes, anthraquinones, minerals, carbohydrates, proteins, hormones, saponins, and lignin are found in aloevera plants. Aloevera has anti-inflammatory & antibacterial properties therefore it can decrease acne & reduce skin inflammation. Saponins, flavonoids, and tannins are antimicrobial substances found in hibiscus flowers (Hibiscus rosa sinensis L.). The best antibacterial activity is seen in neem (A. indica) leaf extract. This study provides information that A. indica leaves have an anti-P. Acne activity which causes acne. Based on this research, herbal antiacne face serum significantly inhibited Staphylococcus aureus and Staphylococcus epidermis growth without causing any irritation.
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