The effects of Cheonggukjang (Korean traditional fermented soybean soup) and Cheonggukjang added with Job's tears were investigated on anticoagulation activities and lipid metabolism in adult male rats fed on high fat diet for 4 weeks. Five treatment groups(n=12 each) were the high fat control group(A) fed on AIN‐93G added with lard(5%) and cholesterol (0.5%), high fat control diet added with 9%(B), 18%(C), 27%(D) and 36%(E) of Job's tears, respectively. There were no significant changes in body weight gain and body fat amount, but food intake was significantly lower in Job's tears added groups than that of the control(p<0.05). In terms of anticoagulation activities, bleeding time was not different, but activated partial thromboplastin time and prothrombin time were siginificantly different(p<0.05). Serum total‐cholesterol and free cholesterol, and phospholipid levels in Job's tears added diet groups were lower(p<0.05). HDL‐cholesterol level was the lowest in group E(p<0.05), but triglyceride and LDL‐ cholesterol levels did not differ among the groups. Atherogenic index tended to be lower in Job's tears added groups except for group E. From the findings, cheonggukjang diet added with Job's tears seemed to be effective in improving blood lipid profile and anticoagulant activity.