Hexokinase 1 Research Articles

Protein kinase activity of rat brain hexokinase

Hexokinase 1 (HK1) purified from rat brain exhibits protein kinase activity, including autophosphorylation and phosphorylation of histone H2A. This protein kinase activity is observed only in the absence of the HK1 carbohydrate substrate, glucose. Analysis of the ATP-binding domains of the mammalian HK1 protein sequences shows significant homology with other mammalian protein kinases.

Regional mapping of the locus for hexokinase-1 (HK1) to 10p11 equals to q23 by gene dosage in human fibroblasts.

The hexokinase (HK) activity in human fibroblasts was close to that expected for a gene dosage effect in a mosaic cell line with about 86% trisomy 10 cells (64% greater than four control lines with normal karyotypes). There was no dosage effect for HK in the cell line that was trisomic for 10q24→qter, nor in the cell line monosomic for 10pter→p12. The data suggest an assignment of the HK1 locus (the only hexokinase in fibroblasts) to 10p11→q23 by exclusion.