A procedure was carried out to transfer to an A or B chromosome of wheat (Triticum aestivum L.), a segment of chromosome 2D carrying the gene Sr6 for resistance to stem rust (Puccinia graminis tritici Eriks. and Henn.). The objectives were to make Sr6 available for breeding in durum wheat (Triticum turgidum L.) and to study dosage effects with Sr6. Plants were produced that had 14 pairs of chromosomes (the A and B genomes) plus an added chromosome 2D carrying Sr6, either as a whole chromosome or as an isosome or telosome. Seeds from these plants were irradiated with thermal neutrons and the M1 progeny were used as male parents in crosses with the durum cultivar Kubanka. Progeny that carried Sr6 and were resistant to stem rust were examined cytologically for the presence of translocations. Five translocations were obtained and then transferred to the hexaploid level by backcrossing. Homozygous lines were produced and four were analyzed in crosses with the Chinese Spring monosomics. In two of them the segment of the A or B genome chromosome had apparently been lost as a result of crossing over. In a third line the translocation involved 2D and its homoeologue 2A. In the final line it appeared that a segment of 2D had been inserted into chromosome 7B. When the chromosome carrying this translocation was transferred to the hexaploid level a position effect occurred that affected the expression of Sr6.