The purpose of this study was to determine the absorption of hexachlorophene (HCP) through normal human skin while washing with detergent-based skin cleansing products containing 3% HCP. A group of human volunteers was initially screened to rule out any skin or systemic disease. One group was assigned Hyperphaze and the other pHisohex. Each subject washed his or her body (excluding scalp) daily for 3 min with 30 ml (containing 900 mg HCP) of the respective detergent-based skin cleansing product and rinsed in the shower in his or her normal manner. Final statistical analysis of data was conducted on 36 subjects (18 for Hyperphaze and 18 for pHisohex randomly selected). During the 8-wk usage test, blood concentrations of hexachlorophene (ng/ml) reached a peak in weeks 3, 4, and 5 (pHisohex means = 543.8, 638.7, and 616.3, respectively; Hyperphaze means = 633.1, 655.3, and 592.6, respectively) and showed a consistent difference between products in weeks 6,7, and 8 ( p < 0.01) pHisohex means = 508.7, 417.2, and 395.4, respectively; Hyperphaze means = 398.8, 332.5, and 330.8, respectively). Hyperphaze resulted in a lower concentration during this period than pHisohex and returned to normal values within 2–3 wk after ending product usage. Throughout this study, no adverse systemic effects related to either product was noted. There was no correlation between whole blood concentrations of hexachlorophene and body surface area or skin pigmentation.