An experiment was carried to estimate heterotic performances of newly developed test hybrids utilizing CMS system during Rabi 2011 using RCBD design. Five known CMS lines and sixteen developed R-lines were crossed through line × tester analysis. Eighty test hybrids were developed. It is very difficult to find a hybrid earlier to BRRI dhan28 as life cycle of 140 days. Heterosis for days to first flowering varied from -6.23% to 16.35% with a SD of 4.47 meanwhile heterosis for days to 80% flowering varied from -9.24% to 11.04% with a SD of 4.03. Among eighty crosses seventy two were found positive heterosis for days to first flowering but sixty four crosses were found for days to 80% flowering. RG-BU08-016R crosses with IR58025A (-2.68*) and IR62820A (-3.42**) showed significant negative standard heterosis for days to first flowering. Five crosses of IR58025A showed significant negative heterosis with RG-BU 08-005R (-3.42*), RG-BU 08-006R (-5.28**), RG-BU 08-013R (-4.129**), RG-BU 08-016R (-5.78**), RG-BU 08-018R (-4.06*) for days to 80% flowering. Besides these 4 crosses of IR62820A showed significant negative heterosis with RG-BU 08-007R (-2.32*), RG-BU 08-016R (-6.03*), RG-BU 08-018R (-4.58**) followed by GAN 46A/RG-BU 08-006R (-4.58*), BRRI 1A/RG-BU 08-001R (-2.55**) and BRRI 1A/RG-BU 08-006R (-9.24**) resulted significant negative heterosis for days to 80% flowering. Positive heterosis were recorded for IR58025A with RG-BU 08-007R (26.97**), RG-BU 08-0046R (8.77**) and RG-BU 08-105R (14.44**) for PER and RG-BU 08-018R (42.46**), RG-BU 08-046R (112.30**), RG-BU 08-057R (27.46*) for SER. While two R-lines, RG-BU 08-013R (25.41**) and RG-BU 08-057R (7.21*) were found having significant positive heterosis for panicle exertion rate crosses with IR62820A but RG-BU 08-025R (52.82**), RG-BU 08-057R (122.66**) and RG-BU 08-063R (37.38**) were found having significant positive heterosis for SER. IR62820A/RG-BU08-057R showed significant positive heterosis for both panicle and stigma exertion rate. GAN46A/RG-BU 08-063R (5.65* & 133.02**) was recorded showing significant positive heterosis for both panicle and stigma exertion rate where. But the crosses of IR68888A was not shown exhibiting a great contribution for panicle and stigma exertion rate except RG-BU08-018R (25.94**). Meanwhile the crosses of BRRI1A was recorded having significant positive heterosis for PER with RG-BU08-005R (13.41**), RG-BU08-007R (5.65*) and RG-BU08-097R (25.94**) as well as RG-BU08-007R (133.02**), RG-BU08-013R (48.19**) and RG-BU08-038R (29.21*) were recorded having significant positive heterosis for SER.