Accurate localization or tracking of wireless devices is a crucial requirement for many emerging location-aware systems. Fields of applications include search and rescue, medical care, intelligent transportation, locationbased billing, security, home automation, industrial monitoring and control, location-assisted gaming, and social networking. During the last few years, there have been intensive research activities in this area and various solutions have been investigated. The main trend now is toward the integration of heterogeneous technologies to ensure global coverage and high accuracy in all possible scenarios, leading to a seamless localization system available anywhere anytime. While satellite-based navigation is well consolidated for open sky scenarios, localization in harsh environments (e.g., indoor or in urban canyons) is still an open issue that requires complementary wireless networks. Cellular systems, local/personal area networks, ad hoc, and wireless-sensor networks can be configured to support localization functionality. Indoor environments, however, are particularly challenging because of severe multipath and non-line-of-sight (NLOS) propagation. In this context, advanced signal processing algorithms must be employed in order to guarantee positioning robustness, such as NLOS identification and mitigation, fusion of data from different sources, and Bayesian methods to enclose any a priori information (e.g., dynamic models for mobile positioning). An important area of research is cooperative localization, which is expected to significantly improve both accuracy and coverage by exploiting all the available measurements on a peer-to-peer basis; efficient protocols and procedures have to be designed to minimize communication overheads and energy consumption. Measurement campaigns are essential for calibrating signal models and testing localization algorithms. A valuable tool for benchmarking algorithms is also provided by fundamental performance bounds, which are being actively analyzed as guidelines for the design of efficient positioning systems. The objective of this special issue, which was promoted under the auspices of the EC Network of Excellence in Wireless Communications NEWCOM++ (in particular, the Work Package WPR.B on Localization and Positioning Techniques), was to gather recent advances in both signal processing and communications areas, for localization in mobile wireless and sensor networks. Articles were solicited on both experimental and theoretical aspects, including new positioning algorithms and methodologies, system design and configuration, performance analysis and measurement campaigns. We received a total of 56 manuscripts addressing the above issues and challenges, of which 16 were selected for publication. Selection of each article was the result of a careful assessment by at least two (mostly three) independent reviewers with expertise on localization and wireless networking. Articles went through a minimum of two to a maximum of four revision phases before acceptance. Accepted articles belong to four main research areas: integration of positioning and communication functionalities, robustness to NLOS errors, indoor positioning, and localization in wireless sensor networks (WSNs). The first group of articles deals with the interaction of positioning and communications at different layers of the protocol stack. Connectivity issues are studied in Gao et al., which considers the relation between distance and communication hops, accounting for the border effect and dependence problems, for a model that is more realistic than the traditional unit-disk graph model. A related problem is investigated in the study of Moragrega et al., which deals with location-aware cluster formation. The authors propose LACFA, a distributed network formation algorithm that significantly increases the probability of localization of sensors in a cluster-tree topology. On the physical layer, Schmeink et * Correspondence: nicoli@elet.polimi.it Dipartimento di Elettronica e Informazione, Politecnico di Milano, Milan, Italy Full list of author information is available at the end of the article Nicoli et al. EURASIP Journal on Wireless Communications and Networking 2011, 2011:197 http://jwcn.eurasipjournals.com/content/2011/1/197