Heterocyclic Aromatic Rings Research Articles

Integrated Electrochemical Oxidation and Biodegradation for Remediation of a Neonicotinoid Insecticide Pollutant.

A novel integrated electrochemical oxidation (EO) and bacterial degradation (BD) technique was employed for the remediation of the chloropyridinyl and chlorothiazolyl classes of neonicotinoid (NEO) insecticides in the environment. Imidacloprid (IM), clothianidin (CL), acetamiprid (AC), and thiamethoxam (TH) were chosen as the target NEOs. Pseudomonas oleovorans SA2, identified through 16S rRNA gene analysis, exhibited the potential for BD. In EO, for the selected NEOs, the total percentage of chemical oxygen demand (COD) was noted in a range of 58-69%, respectively. Subsequently, in the biodegradation of EO-treated NEOs (BEO) phase, a higher percentage (80%) of total organic carbon removal was achieved. The optimum concentration of NEOs was found to be 200 ppm (62%) for EO, while for BEO, the COD efficiency was increased up to 79%. Fourier-transform infrared spectroscopy confirms that the heterocyclic group and aromatic ring were degraded in the EO and further utilized by SA2. Gas chromatography-mass spectroscopy indicated up to 96% degradation of IM and other NEOs in BD (BEO) compared to that of EO (73%). New intermediate molecules such as silanediamine, 1,1-dimethyl-n,n'-diphenyl produced during the EO process served as carbon sources for bacterial growth and further mineralized. As a result, BEO enhanced the removal of NEOs with a higher efficiency of COD and a lower consumption of energy. The removal efficiency of the NEOs by the integrated approach was achieved in the order of AC > CL > IM > TH. This synergistic EO and BD approach holds promise for the efficient detoxification of NEOs from polluted environments.

Treatment of mature landfill leachate using chemical and electrical Fenton with novel Fe-loaded GAC heterogeneous catalysts

In this study, heterogeneous Fenton catalysts were prepared by loading iron oxides on granular activated carbon (GAC), with which as heterogeneous Fenton catalysts, mature landfill leachate (MLL) was treated by chemical and electrical Fenton method. For chemical Fenton treatment of MIL, the optimum conditions were optimised by orthogonal experiments and the removal of COD, NH3-N and colour reached 93.7 %, 65.5 % and 84 % respectively for static experiments, whereas the heterogeneous Fenton fluidized bed reactor removed >90 % of COD in all the 12 h of continuous operation. For electrical Fenton treatment of MIL, the removal rates of COD, colour, NH3-N, and TN were 97.4 %, 99 %, 100 %, and 94.2 %, respectively under the optimum treatment conditions of pH = 5, the GAC/α-FeOOH catalyst dosage of 13.3 g L−1, and the applied voltage of 8 V with the energy consumption of only 0.23 kWh (g COD)−1. Upon characterization, humic acid, fulvic acid and xanthohumic acid in the MLL were completely degraded and the complex macromolecules such as polycyclic aromatic hydrocarbons and heterocyclic rings were reduced and converted to simple small molecules such as alkanes. The quenching experiments verified that the ·OH produced in the heterogeneous electrical Fenton system plays an important role in the degradation of MLL, while the removal of NH3-N also relies on indirect oxidation with the active chlorine produced by the electrochemical system. GAC-based heterogeneous Fenton catalysts with high catalytic capacity and easy recycling have good application prospects in treating MLL.

Selective extraction of aromatics from residual oil with subcritical water

Based on theoretical calculations and experimental characterizations, the extraction of heavy oil components using subcritical water (Sub-CW) as an extraction solvent was studied in the temperature range of 250–325 °C. According to theoretical calculations, small-scale polycyclic aromatic hydrocarbons (PAHs) are preferentially dissolved in Sub-CW. Meanwhile, the dissolution of heavy oil molecules with large-scale aromatic structures and heterocyclic rings in Sub-CW is hindered. The increase in temperature reduces the hydrogen bonding force between water molecules and the van der Waals repulsion between Sub-CW and PAHs, promoting the dissolution of PAHs in Sub-CW. Extraction experiments applied in a semi-continuous device confirm that Sub-CW selectively extracts aromatic hydrocarbons from heavy oil. Increasing extraction temperature increases the yield of the extract. The aromatic hydrocarbon content in the extract at 325 °C reaches 80 wt%, which is 27 wt% higher than feedstock value. In addition, the asphaltene content in the extract is reduced from the feedstock value of 14 wt% to 2 wt%.

Molecular docking screening, dynamics simulations, ADMET, and semi-synthesis prediction of flavones and flavonols from the COCONUT database as potent bifunctional neuraminidase inhibitors

Finding new neuraminidase (N) inhibitors to improve anti-influenza treatment is necessary because of the high mutation rates of N protein. Over 3,000 flavones/flavonols and their synthesized products from the COCONUT database were performed in silico docking screening with N1-H274Y-oseltamivir protein (PDB ID: 3CL0). Several derivatives containing nitrogen heterocyclic groups or aromatic rings showed higher anti-neuraminidase potential than that of laninamivir. Especially, the linker groups between the flavone aglycone and nitrogen heterocyclic group created the interactions with the triad of arginine residues Arg118-Arg292-Arg371, which suggested these compounds could become bifunctional inhibitors against the influenza virus strains at the sialic acid binding site and the adjacent 430-cavity position through triad of arginine residues binding. ADMET indicators and the synthesis design strategy of the most suitable compound, ethyl 4-{2-[(5-hydroxy-4-oxo-2-phenyl-4H-chromen-7-yl)oxy]acetyl}piperazine-1-carboxylate, were also successfully predicted and it could be a concerned candidate for further wet-lab synthesis, in vivo and clinical study.

Schiff bases-based metallo complexes and their crucial role in the realm of pharmacology. A review

Three prominent health challenges affecting our society include cancer, diabetes, and antibiotic-resistant infections. Despite the availability of conventional drugs for these conditions, the pervasive problem of multidrug resistance significantly diminishes their effectiveness. Consequently, it is paramount to prioritize the development of novel drug compounds endowed with improved and desirable therapeutic properties. Utilizing metal-based drugs has proven to be an effective strategy in the realm of anticancer treatment and this approach holds significant promise for the enhanced treatment of multi-resistant infections and diabetes. Schiff bases, being bioactive organic compounds, have attracted considerable attention. Researchers have been actively exploring the potential therapeutic applications of metallo-based Schiff bases, including heterocyclic molecules and/or aromatic rings adorned with diverse functional groups across various illnesses. This review catalogs (2010 to 2023) the classification of metal-based Schiff base compounds that incorporate heterocyclic rings and/or aromatic rings with diverse functional groups, which possess antimicrobial, antidiabetic, and anticancer qualities. These insights can assist the medical chemist in identifying suitable drug candidates for developing innovative pharmaceuticals.

Microbiome based approaches for the degradation of polycyclic aromatic hydrocarbons (PAHs): A current perception

Globally, polycyclic aromatic hydrocarbons (PAHs) pollution is primarily driven by their release into the air through various combustion processes, including burning fossil fuels such as coal, oil, and gas in motor vehicles, power plants, and industries, as well as burning organic matter like wood, tobacco, and food in fireplaces, cigarettes, and grills. Apart from anthropogenic pollution sources, PAHs also occur naturally in crude oil, and their potential release during oil extraction, refining processes, and combustion further contributes to contamination and pollution concerns. PAHs are resistant and persistent in the environment because of their inherent features, viz., heterocyclic aromatic ring configurations, hydrophobicity, and thermostability. A wide range of microorganisms have been found to be effective degraders of these recalcitrant contaminants. The presence of hydrocarbons as a result of numerous anthropogenic activities is one of the primary environmental concerns. PAHs are found in soil, water, and the air, making them ubiquitous in nature. The presence of PAHs in the environment creates a problem, as their presence has a detrimental effect on humans and animals. For a variety of life forms, PAH pollutants are reported to be toxic, carcinogenic, mutation-inducing, teratogenic, and immune toxicogenics. Degradation of PAHs via biological activity is an extensively used approach in which diverse microorganisms (fungal, algal, clitellate, and protozoan) and plant species and their derived composites are utilized as biocatalysts and biosurfactants. Some microbes have the ability to transform and degrade these PAHs, allowing them to be removed from the environment. The goal of this review is to provide a critical overview of the existing understanding of PAH biodegradation. It also examines current advances in diverse methodologies for PAH degradation in order to shed light on fundamental challenges and future potential.

Emission of water soluble brown carbon from different combustion sources: optical properties and functional group characterisation

Brown carbon (BrC) has a large impact on the Earth’s radiative balance. This study examined BrC’s optical characteristics, chemical functional groups, and total organic carbon (TOC) in deposited particulate matter from combustion of biomass, fossil fuels, and mosquito coil. A UV–vis spectrophotometer was used to assess BrC’s optical properties, a TOC analyzer was used to quantify TOC, and attenuated total reflection-fourier transform infrared spectroscopy (ATR-FTIR) was used to identify functional group. The light absorption properties (absorption coefficient (babs-BrC), mass absorption efficiency (MAE365), absorption angstrom exponent (AAE), and refractive index (kabs-BrC)) were determined. Among biomasses, wheat straw exhibited the greatest value of babs-BrC (27.26 × 103 ± 4.09 × 103 Mm−1) whereas maize straw had the lowest value of babs-BrC (7.38 × 103 ± 1.11 × 103 Mm−1). A higher light absorption coefficient at 365 nm suggested that chromophores contributed more to the process. AAE followed this sequence - mosquito coil (5.46 ± 0.82) > biomass (5.13 ± 1.45) > fossil fuels (2.85 ± 0.32). The highest value of MAE365 (0.99 ± 0.15 m2 gC−1) and kabs-BrC (0.028 ± 0.004) was obtained in wheat straw, whereas the lowest value of MAE365 (0.07 ± 0.011 m2 gC−1) and kabs-BrC (0.002) was identified in octane. Biomasses samples showed a strong peak at ∼850, ∼1368, ∼1370, ∼1306, and near 1640 cm−1, indicating that the major component of BrC was organic nitrate R-ONO2 which is responsible to increase the light absorption properties. It also contains complex heterocyclic aromatic ring compounds, carbonyl group (C=O), and nitrogen-containing groups such as −NO 2, C≡N. The projected substantial contribution of BrC to overall light absorption is one illustration of the probable climatic effect of biomasses and fossil fuel burning in Southeast Asian region.

Recent trends in polycyclic aromatic hydrocarbons pollution distribution and counteracting bio-remediation strategies

Polycyclic aromatic hydrocarbons (PAHs) are distributed worldwide due to long-term anthropogenic pollution sources. PAHs are recalcitrant and highly persistent in the environment due to their inherent properties, such as heterocyclic aromatic ring structures, thermostability, and hydrophobicity. They are highly toxic, carcinogenic, immunotoxic, teratogenic, and mutagenic to various life systems. This review focuses on the unique data of PAH sources, exposure routes, detection techniques, and harmful effects on the environment and human health. This review provides a comprehensive and systematic compilation of eco-friendly biological treatment solutions for PAH remediation, such as microbial remediation approaches utilizing microbial cultures. In situ and Ex situ bioremediation of PAH methods, including composting land farming, biopiles, bioreactors bioaugmentation, and phytoremediation processes, are discussed in detail, as is a summary of the factors affecting and limiting PAH bioremediation. This review provides an overview of emerging technologies that use multi-process combinatorial treatment approaches and answers to generating value-added by-products during PAH remediation.

A Review on Synthetic Strategies for Useful Oxadiazole Derivatives in Psychological Disorders

Abstract: Oxadiazole is a five-membered aromatic heterocyclic ring having two nitrogen and one oxygen atom. Various isomeric forms have been reported for oxadiazole, such as 1,3,4-oxadiazole, 1,2,4-oxadiazole, 1,2,5-oxadiazole, etc. Currently, a considerable population worldwide is facing several mental problems due to a competitive lifestyle. The present status of available medicines do not promise complete cure without any adverse effects. Therefore, these disorders are continuously challenging the researchers to come up with new molecules with superior efficacy and minimum side effects. All the isomeric forms of oxadiazole have numerous potential in treating various mental problems such as Parkinsonism, Alzheimer’s, schizophrenia, and epileptic disorders. In this review article, we summarize several recently reported synthetic strategies for preparing different oxadiazole and its derivatives which were found effective in psychological disorders. The researchers will be able to obtain the necessary information (synthesis strategies) through this article for their future research on new molecules containing the oxadiazole moiety. Furthermore, this review article will help the researchers in the fight against mental disorders and highlights possible molecules for the treatment of mentally challenged people.

In vitro metabolism of 2-methoxy-N-[3-[4-[3-methyl-4-[(6-methyl-3-pyridinyl)oxy]anilino]-6-quinazolinyl]prop-2-enyl]acetamide (CP-724,714) by aldehyde oxidase and predicting its percent contribution relative to CYP-mediated metabolism.

2-methoxy-N-[3-[4-[3-methyl-4-[(6-methyl-3-pyridinyl)oxy]anilino]-6-quinazolinyl]prop-2-enyl]acetamide (CP-724,714) is an anticancer drug that was discontinued owing to hepatotoxicity found in clinical studies. Metabolite analysis of CP-724,714 was conducted using human hepatocytes, where twelve oxidative metabolites and one hydrolyzed metabolite were formed. Among the three mono-oxidative metabolites, the formation of two was inhibited by adding 1-aminobenzotriazole, a pan-CYP inhibitor. In contrast, the remaining one was not affected by this inhibitor but partially inhibited by hydralazine, indicating that aldehyde oxidase (AO) was involved in metabolizing CP-724,714, which contains a quinazoline substructure, a heterocyclic aromatic quinazoline ring, known to be preferably metabolized by AO. One of the oxidative metabolites of CP-724,714 observed in human hepatocytes was also generated in recombinant human AO. Although CP-724,714 is metabolized by both CYPs and AO in human hepatocytes, the contribution level of AO could not be evaluated using its specific inhibitors because of low AO activity in in vitro human materials. Here, we present a metabolic pathway for CP-724,714 in human hepatocytes and the involvement of AO in CP-724,714 metabolism. We showed here a plausible workflow for predicting AO contribution to the metabolism of CP-724,714 based on DMPK screening data. Significance Statement CP-724,714 was identified as a substrate of aldehyde oxidase (AO) rather than xanthine oxidase. Since CP-724,714 is also metabolized by CYPs, the contribution levels of AO and CYPs in the metabolism of CP-724,714 were estimated simultaneously based on in vitro drug metabolism screening data.

Five-Membered Heterocyclic Sulfonamides as Carbonic Anhydrase Inhibitors

The development of heterocyclic derivatives has progressed considerably over the past decades, and many new carbonic anhydrase inhibitors (CAIs) fall into this field. In particular, five-membered heterocyclic sulfonamides have been generally shown to be more effective inhibitors compared to six-membered rings ones. Despite the importance of oxygen and nitrogen five-membered heterocyclic aromatic rings in medicinal chemistry, the installation of sulfonamide moiety on such heterocycles has not received much attention. On the other hand, 1,3,4-thiadiazole/thiadiazoline ring-bearing sulfonamides are the scaffolds which have been widely used in a variety of pharmaceutically important CAIs such as acetazolamide, metazolamide and their many derivatives obtained by using the tail approach. Here, we reviewed the field focusing on the diverse biological activities of these CAIs, such as antiglaucoma, antiepileptic, antitumor and antiinfective properties. This review highlights developments involving five-membered heterocyclic sulfonamides over the last years, with a focus on their pharmacological/clinical applications.

Chlorinated nucleotides and analogs as potential disinfection byproducts in drinking water

Identification of emerging disinfection byproducts (DBPs) of health relevance is important to uncover the health risk of drinking water observed in epidemiology studies. In this study, mutagenic chlorinated nucleotides were proposed as potential DBPs in drinking water, and the formation and transformation pathways of these DBPs in chlorination of nucleotides were carefully investigated. A total of eleven chlorinated nucleotides and analogs were provisionally identified as potential DBPs, such as monochloro uridine/cytidine/adenosine acid and dichloro cytidine acid, and the formation mechanisms involved chlorination, decarbonization, hydrolysis, oxidation and decarboxylation. The active sites of nucleotides that reacted with chlorine were on the aromatic heterocyclic rings of nucleobases, and the carbon among the two nitrogen atoms in the nucleobases tended to be transformed into carboxyl group or be eliminated, further forming ring-opening or reorganization products. Approximately 0.2–4.0 % (mol/mol) of these chlorinated nucleotides and analogs finally decomposed to small-molecule aliphatic DBPs, primarily including haloacetic acids, trichloromethane, and trichloroacetaldehyde. Eight intermediates, particularly chlorinated imino-D-ribose and imino-D-ribose, were tentatively identified in chlorination of uridine. This study provides the first set of preliminary evidence for indicating the promising occurrence of chlorinated nucleotides and analogs as potential toxicological-relevant DBPs after disinfection of drinking water.

Development of Benzobisoxazole-Based Novel Conjugated Polymers for Organic Thin-Film Transistors.

Benzo[1,2-d:4,5-d']bis(oxazole) (BBO) is a heterocyclic aromatic ring composed of one benzene ring and two oxazole rings, which has unique advantages on the facile synthesis without any column chromatography purification, high solubility on the common organic solvents and planar fused aromatic ring structure. However, BBO conjugated building block has rarely been used to develop conjugated polymers for organic thin film transistors (OTFTs). Three BBO-based monomers, BBO without π-spacer, BBO with non-alkylated thiophene π-spacer and BBO with alkylated thiophene π-spacer, were newly synthesized and they were copolymerized with a strong electron-donating cyclopentadithiophene conjugated building block to give three p-type BBO-based polymers. The polymer containing non-alkylated thiophene π-spacer showed the highest hole mobility of 2.2 × 10-2 cm2 V-1 s-1, which was 100 times higher than the other polymers. From the 2D grazing incidence X-ray diffraction data and simulated polymeric structures, we found that the intercalation of alkyl side chains on the polymer backbones was crucial to determine the intermolecular ordering in the film states, and the introduction of non-alkylated thiophene π-spacer to polymer backbone was the most effective to promote the intercalation of alkyl side chains in the film states and hole mobility in the devices.

Synergistically and sustainably performed inhibitors for galvanised steel against aqueous corrosion

This work develops a synergistic inhibiting strategy through employing two distinct aromatic heterocyclic ring systems, i.e., 2-amino-4-methylthiazole (2AT-Me) and 2-(methylthio)imidazole (I-2MT) to provide electro-galvanised steel (ZE) synergistic and sustainable protection against aqueous corrosion. The formulated mixture rendered an enhanced inhibitory efficiency of 96%, reinforcing the corrosion resistance of ZE after 7-day immersion in 0.1 M NaCl solution. Analysis of surface chemistry and electrolyte chemistry reveals that the growth dynamic of the protective layer is a two-step process reflecting the sequential responses of each inhibitor’s characteristic mode-of-action. Such a synergistic inhibition formulation provides promises to modify post-corroded ZE surface.

Successive Cleavage and Reconstruction of Lignin β‐O‐4 Models and Polymer to Access Quinoxalines

The construction of N-heterocyclic compounds from lignin remains a great challenge due to the complex lignin structure and the involvement of multiple steps, including the cleavage of lignin C-O linkages and the formation of heterocyclic aromatic rings. Herein, the first example of KOH mediated sustainable synthesis of quinoxaline derivatives from lignin β-O-4 model compounds in a one-pot fashion under transition-metal-free conditions has been achieved. Mechanistic studies suggested that this transformation includes highly coupled cascade steps of cleavage of C-O bonds, dehydrative condensation, sp3 C-H bond oxidative activation, and intramolecular dehydrative coupling reaction. With this protocol, a wide range of functionalized quinoxalines, including an important drug compound AG1295, were synthesized from lignin β-O-4 model compounds and β-O-4 polymer, showcasing the application potential of lignin in pharmaceutical synthesis.

Transition‐Metal‐Free Synthesis of Functionalized Quinolines by Direct Conversion of β‐O‐4 Model Compounds

AbstractDirect production of heterocyclic aromatic compounds from lignin β‐O‐4 models remains a huge challenge due to the incompatible catalysis for aryl ether bonds cleavage and heterocyclic ring formation. Herein, the first example of quinoline synthesis from β‐O‐4 model compounds by a one‐pot cascade reaction is reported in yields up to 89 %. The reaction pathway involves selective cleavage of C−O bonds, dehydrogenation, aldol condensation, C−N bond formation along with heterocyclic aromatic ring construction. The control experiments suggest that both imine and chalcone were identified as the key intermediates, and the rate determining step as well as the preferred pathway were experimentally clarified and supported by density functional theory (DFT) calculations. Based on this protocol, the conversion of β‐O‐4 polymer delivered 56 wt % yield of quinoline derivative in three steps. This transformation provides a potential petroleum‐independent choice for heterocyclic aromatic chemicals.

Transition‐Metal‐Free Synthesis of Functionalized Quinolines by Direct Conversion of β‐O‐4 Model Compounds

Direct production of heterocyclic aromatic compounds from lignin β-O-4 models remains a huge challenge due to the incompatible catalysis for aryl ether bonds cleavage and heterocyclic ring formation. Herein, the first example of quinoline synthesis from β-O-4 model compounds by a one-pot cascade reaction is reported in yields up to 89 %. The reaction pathway involves selective cleavage of C-O bonds, dehydrogenation, aldol condensation, C-N bond formation along with heterocyclic aromatic ring construction. The control experiments suggest that both imine and chalcone were identified as the key intermediates, and the rate determining step as well as the preferred pathway were experimentally clarified and supported by density functional theory (DFT) calculations. Based on this protocol, the conversion of β-O-4 polymer delivered 56 wt % yield of quinoline derivative in three steps. This transformation provides a potential petroleum-independent choice for heterocyclic aromatic chemicals.

A Short Review on Synthetic Methodologies of Flavonoids

Flavonoids are the pigments present in plants which mostly found in terrestrial plants. Flavonoids are indeed a naturally present group of polyphenolic compounds present in plants. They were driven by the term "flavus," which means "yellow." It is a 15-carbon skeleton compound. They have fused aromatic ring and benzopyran heterocyclic ring having oxygen atom in it along with phenyl substituent. They are synthesized from Phenylalanine. In cereals and Herbs, they are mainly found. Flavonoids are compounds that are biologically active. They provide color and protection from ultraviolet rays. They have many classes based upon oxidative status, number, and types of substituents present. Flavonoids exist naturally in the form of polymers, most commonly in dimers form. They occur primarily in β-glycosides form except for Catechins. They can help in the inhibition of enzymes and stimulate some hormones along with some neurotransmitters. They also show the properties of scavenging free radicals. They can inhibit or kill many bacterial strains, viral enzymes, and pathogenic protozoans. There are various techniques and methods for the synthesis of natural products artificially. In the present study, we have attempted to cover different synthetic methods for flavonoid synthesis to find its best way to synthesize. It was concluded that Baker & Venkatraman synthesis and Claisen-Schmidt condensation are well-known methods used to synthesize flavonoids.

Oxygen and Chlorine Dual Vacancies Enable Photocatalytic O2 Dissociation into Monatomic Reactive Oxygen on BiOCl for Refractory Aromatic Pollutant Removal.

Room-temperature molecular oxygen (O2) dissociation is challenging toward chemical reactions due to its triplet ground-state and spin-forbidden characteristic. Herein, we demonstrate that BiOCl of oxygen and chlorine dual vacancies can photocatalytically dissociate O2 into monatomic reactive oxygen (•O-) for the ring opening of aromatic refractory pollutants toward deep oxidation. The electron-rich and geometry-flexible dual vacancies of oxygen and chlorine remarkably lengthen the O-O bond of adsorbed O2 from 1.21 to 2.74 Å, resulting in the rapid O2 dissociation and the subsequent •O- formation. During the photocatalytic degradation of sulfamethazine, the in situ-formed •O- plays an indispensable role in breaking the critical intermediate of pyrimidine containing a stubborn aromatic heterocyclic ring, thus facilitating the overall mineralization. More importantly, BiOCl of oxygen and chlorine dual vacancies is also superior to its monovacancy counterparts on the degradation of other refractory pollutants containing conjugated six-membered rings, including p-chlorophenol, p-chloronitrobenzene, p-hydroxybenzoic acid, and p-nitrobenzoic acid. This study sheds light on the importance of sophisticated defects for regulating the O2 activation manner and deliveries a novel O2 activation approach for environmental remediation with solar energy.

Preparation of Kenaf Biochar and Its Adsorption Properties for Methylene Blue

The toxic dyestuffs from printing and dyeing wastewater have caused serious damages to the ecological environment, thus exploring effective methods to remove them having become a key topic. Here, a series of biochar samples were synthesized form kenaf to adsorb methylene blue (MB), which was acted as the dye representative for the test of adsorption capacity due to the presence of abundant double bond and aromatic heterocyclic ring. By tuning the raw materials and pyrolysis temperature, a super adsorption capacity about 164.21 mg·g–1 was obtained over the biochar that pyrolyzed at 700°C with the kenaf fiber as raw material. Through the physical adsorption, elemental analysis, FTIR spectra and NH3-TPD, it was found the high surface area and pore volume of biochar played a key role in the adsorption of MB, and the acidic sites would also assist the adsorption process. Besides, the adsorption kinetic model was fitted and calculated, implying the MB physically adsorbed on the biochar rapidly and then occurred chemical adsorption on the acidic sites. In addition, through KBC700 recycling experiments, it was found that kenaf biochar had a good binding force to MB, which effectively avoided secondary pollution. This work provides important insights for the adsorption mechanism of MB by biochar, also offers some guidance for the further synthesis of biochar from various biomass.