Introduction The purpose of this study was to compare dentinal microcrack formation while using hand files (HFs), 4 brands of nickel-titanium (NiTi) rotary files and the self-adjusting file. Methods One hundred forty mandibular first molars were selected: 20 teeth were left unprepared and served as control, and the remaining 120 teeth were divided into 6 groups. HFs, HERO Shaper (HS; Micro-Mega, Besancon, France), Revo-S (RS, Micro-Mega), Twisted File (TF; SybronEndo, Orange, CA), ProTaper (PT, Dentsply Maillefer), and SAFs were used to prepare the 2 mesial canals. Roots were then sectioned 3, 6, and 9 mm from the apex, and the cut surface was observed under a microscope and checked for the presence of dentinal microcracks. Results The control, HF, and SAF groups did not show any microcracks. In roots prepared with the HS, RS, TF, and PT, dentinal microcracks were observed in 60%, 25%, 44%, and 30% of teeth, respectively. There was a significant difference between the control/HF/SAF group and the 4 NiTi rotary instrument groups ( P < .0001). However, no significant difference was found among the 4 NiTi rotary instruments ( P > .005). Conclusions All rotary files created microcracks in the root dentin, whereas the SAF file and hand instrumentation presented with satisfactory results with no dentinal microcracks.
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