The discovery of DNA (Deoxyribo Nucleic Acid) is considered to be the greatest discovery which revolutionized the whole genetical analysis system straight away. This unique hereditary material is considered as one of the best ways of proofing scientific elements. Therefore, it can be directly utilized to identify victims and perpetrators in crime scene investigations and resolve civil cases. Even though DNA fingerprinting technology is utilized in Sri Lanka, criminal and civil investigations consumed more time and still delaying the results of the investigation procedures. Therefore, this research was conducted to find out the current status of DNA fingerprinting procedures in criminal and civil investigations in Sri Lanka. Secondary analysis and in-depth interview methods were used as a mode of data collection and the Government Analyst’s Department of Sri Lanka was selected as the study site based on the most recent DNA analyses, randomly selected 100 negative and positive DNA records were extracted from five different biological samples which cover up four different crimes and civil cases. Further, 08 scientists of the DNA Section were interviewed through an interview and data was analyzed via Microsoft Excel Application. It was revealed that blood and buccal cells are the most commonly used samples for DNA extractions and the majority of the cases have been matched with suspects and victims with 100% of accuracy in criminal and civil investigations. Contemporary DNA typing and profiling techniques are currently utilized in Sri Lanka and this department is not affected by any outside interferences and work independently. The current DNA fingerprinting technology has improved significantly and has a positive influence on criminal and civil investigations. However, the recommendations suggested by the DNA analysts should be considered and incorporate for the DNA fingerprinting technology to be successful in Sri Lanka. Further, public should be made aware of the benefits and the importance of DNA databases. In an anthropological perspective, a gradual increase in establishing parental and child support litigation can be recognized since DNA fingerprinting technology is the only way to prove such kind of incidents.