Herbicides In Soil Research Articles

Organomineral Interactions and Herbicide Sorption in Brazilian Tropical and Subtropical Oxisols under No-Tillage.

We evaluated the effects of the soil organic matter (SOM) composition, distribution between soil aggregates size, and their interactions with the mineral phase on herbicide sorption (alachlor, bentazon, and imazethapyr) in tropical and subtropical Oxisols under no-till systems (NT). Using soil physical fractionation approach, sorption experiments were performed on whole soils and their aggregates. SOM chemistry was assessed by CP/MAS (13)C NMR. The lower sorption observed in tropical soils was attributed to the greater blockage of SOM sorption sites than in subtropical soils. When these sites were exposed upon physical fractionation, sorption of the three herbicides in tropical soils increased, especially for imazethapyr. High amounts of poorly crystallized sesquioxides in these soils may have contributed to masking of sorption sites, indicating that organomineral interactions may lead to blockage of sorption sites on SOM in tropical soils.

Características fisiológicas do feijoeiro cultivado em solos após fitorremediação do picloram

A fitorremediação tem sido relatada como técnica promissora para a descontaminação de solos com resíduos de herbicidas. Para herbicidas hormonais, há dificuldade na determinação de resíduos pela técnica do bioensaio em função da variação na resposta em crescimento apresentada pela indicadora. Objetivou-se com esse trabalho avaliar o efeito de subdoses do picloram, em solos fitorremediados com braquiária, nas características fisiológicas do feijoeiro. O ensaio foi realizado em ambiente protegido no delineamento de blocos ao acaso. Aplicou-se diferentes doses do picloram (0; 2; 4; 8; 16 e 32 g ha-1), sendo posteriormente cultivado com braquiária por 120 dias. Nesta data retirou-se a braquiária dos vasos e semeou o feijão como planta bioindicadora, sendo depois de 30 dias avaliada as características fisiológicas e a matéria seca do feijoeiro. Mesmo após a remediação por plantas de braquiária, as subdoses do picloram afetaram as características fisiológicas do feijoeiro, principalmente a taxa fotossintética e a eficiência no uso da água, sendo essas variáveis as mais indicadas para se avaliar o resíduo do herbicida no solo. A redução dessas variáveis promoveu menor acúmulo de biomassa total das plantas de feijão nos solos fitorremediados. A segunda maior dose do herbicida provocou também redução da relação Fv/Fm abaixo de 0,75, indicando algum tipo de estresse do aparato fotossintético das folhas do feijoeiro.

Field dissipation of pendimethalin and alachlor in sandy clay loam soil and its terminal residues in sunflower (Helianthus annus L.)

Field experiments were conducted with sunflower as a test crop during 2010-11 to study the dissipation kinetics and the persistence of pendimethalin and alachlor in sandy clay loam soil and its terminal residues in sunflower. Herbicides were applied at recommended and double the recommended dose along with control and the treatments were replicated thrice in randomized block design. The soil and plant samples collected at periodical intervals for herbicides residue determination using GC equipped with ECD detector. Results shows that the degradation of both the herbicides in soil was faster at higher dose of application than at the lower dose and the concentration decreased with the advancement in crop growth. While pendimethalin persisted in soil for 60 – 90 days, the alachlor persisted in soil for 30 - 45 days depending on the quantity of application. Degradation of both the herbicides in soil followed first order kinetics with the mean half life of 14.6 and 9.8 days respectively for pendimethalin and alachlor. Residues of these herbicides were below 0.001 mg/kg at the time of harvest in soil, sunflower seeds and stalks showed that these herbicides a can be safely used for the control of weeds in sunflower cultivation.

Formation of yield capacity and sowing qualities of soybean seeds affected by current herbicides

The issue of herbicide influence on the basic sowing qualities of soybean seeds grown with their application is topical. The aim and tasks of the study. Offering of soil and post-emergence herbicides permitted for use in soybean crops has grown in recent years. However, effects of these herbicides on the basic sowing qualities of soybean seeds grown with their application have not been studied. To establish productivity parameters, germination energy and laboratory germination capacity of soybean seeds grown with application of soil and post-emergence herbicides. Material and methods. The experiment was laid out on typical black soil. Soil herbicides were applied before sprouting, and post-emergence ones - after soybean sprouting. The size of the registration plot was 36 m 2 ; the experiment was repeated in three replicas. Soybean was harvested wit a harvester «Sampo-130. Sowing qualities of soybean seeds were evaluated by specialists from the Laboratory Seed Production and Seed Investigations of the Plant Production Institute nd. a V. Ya. Yuriev of NAAS of Ukraine. Results and Discussion. Pre-emergence application of Harness herbicide and post-emergence application of tank mixtures from agents Nabob (1.0 L / ha) + Fabian (50 g / ha) + Miura (0.6 L / ha) and Nabob (1.5 L / ha) + Fusilade Forte 150 EC (0.8 L / ha) provided the highest soybean yields. Moisture regimen in some study years influenced the yield capacity and sowing qualities of soybean seeds. Soybean seeds grown with pre-emergence (100 g / ha) and post-emergence (70 g / ha) application of Fabian herbicide in tank mixture with graminicides Fusilade Forte 150 EC (0.6 L / ha), Panther (0.8 L / ha) and Miura (0.4 L / ha) had low germination energy. Soil and post-emergence herbicides did not significantly affect the laboratory germination capacity of soybean seeds. Conclusions. Among soil herbicides, Harness agent provided the maximum soybean yield, and among post-emergence ones tank mixtures from agents Nabob + Fabian + Miura and Nabob + Fusilade Forte 150 EC stood out. Pre-emergence and post-emergence application of Fabian in tank mixture with graminicides reduced germination energy of soybean seeds. No significant effect of herbicides on the laboratory germination capacity of soybean seeds was found.

Potential impacts of seasonal variation on atrazine and metolachlor persistence in andisol soil.

To estimate the potential effect of seasonal variation on the fate of herbicides in andisol soil, atrazine and metolachlor residues were investigated through the summer and winter seasons during 2013 and 2014 under field condition. The computed half-lives of atrazine and metolachlor in soil changed significantly through the two seasons of the trial. The half-lives were shorter in summer season with 16.0 and 23.5days for atrazine and metolachlor, respectively. In contrast, the half-lives were longer during the winter season with 32.7 and 51.8days for atrazine and metolachlor, respectively. The analysis of soil water balance suggested that more pesticide was lost in deeper soil layers through infiltration in summer than in winter. In addition, during the summer season, metolachlor was more likely to leach into deeper soil layer than atrazine possibly due to high water solubility of metolachlor.

Role of different earthworms in a soil polluted with oxyfluorfen herbicide. Short-time response on soil biochemical properties

In this paper we studied in the laboratory the bioremediation effects of an oxyfluorfen herbicide-polluted soil with and without three earthworms (Eisenia fetida, Lumbricus terrestris and Allolobophora molleri) and its influence on soil biochemical properties over 90 days. Glutathione-S-transferase activity (GST) and weight in earthworms were measured at four different incubation times (3, 15, 60 and 90 days). Cocoon numbers, average weight per cocoon and hatchlings per cocoon were measured 30 days after the oxyfluorfen exposure. Herbicide in soils and the earthworms was determined during the incubation period. To observe the effects of bioremediation of the contaminated soil, ATP, and urease, phosphatase and arylsulphatase activities were measured. Although the bioaccumulation factor of oxyfluorfen was 1.4- and 3.5-fold higher in A. molleri than for E. fetida and L. terrestris, respectively, compared to the unpolluted soils, the GST activity of A. molleri significantly decreased by 19% in soils polluted with oxyfluorfen, whereas for E. fetida and L. terrestris the GST activity significantly decreased by 23.3% and 34.2%, respectively. Furthermore, the earthworms’ weight, cocoon numbers, average weight per cocoon and hatchlings per cocoon showed a pronounced decrease among L. terrestris, E. fetida and A. molleri, respectively, in polluted soils. When the concentration of herbicide decreased, so did enzyme activity. This decrease inhibition was more pronounced in soils with A. molleri than E. fetida and L. terrestris. Of the three species tested, A. molleri appears to the best suited for bioremediation of oxyfluorfen-contaminated soil.

Degradation of Herbicide Mesotrione in Three Soils with Differing Physicochemical Properties from China.

The movement and fate of herbicides in soils under various environmental factors are important in evaluating their mobility and ecological impact. The effects of sterilization, solarization, and soil physicochemical properties on the degradation of herbicide mesotrione in three soils from China were evaluated using laboratory incubation method, and the degradation kinetics were also simulated using pseudo first-order reaction model. The calculated half-lives () of mesotrione were found to be 3.78- to 5.24-fold increased in sterilized soils than nonsterilized soils, which indicated that the degradation of mesotrione was strongly affected by soil microbial activity. A certain role of promoting degradation effect of natural light was found, and the values appeared to be only 7.90, 15.89, and 25.29 d in the surface of paddy soil, sandy loess, and silt clay loam, respectively. Correlation analysis between the observed first-order reaction rate constant () values and the selected soil properties revealed that the degradation of mesotrione was highly dependent on soil pH value ( > 0.992) and organic matter content ( > 0.932), but less related with clay content (<0.02 mm) with < 0.761 and nonrelated with cation exchange capacity (CEC) ( < 0.164). Data obtained in this study are helpful for further research on the prediction of the movement and fate of mesotrione in soils in limiting its environmental impact.

Estimating the combined toxicity of flufenacet and imazaquin to sorghum with pore water herbicide concentration

Combined toxicity of herbicides to non-target crops is usually resulted from their successive application. The present study was conducted to assess the combined toxicity of flufenacet (FLU) and imazaquin (IMA) to sorghum with their concentration in soil pore water. The concentrations that inhibited growth by 50% (IC50) of FLU and IMA individually and their combination estimated from the herbicide concentrations in soil pore water notably differed from those based on the amended concentrations, due to the decline in bioavailability resulting from adsorption of the herbicides onto soil. According to the amended concentrations, the combined effect of FLU and IMA in soil on sorghum growth was identified as additive action. Based on the concentration in soil pore water, however, it was determined to be antagonism, which was identical to that observed in a test using culture solution. The results revealed that pore water herbicide concentration might be an effective tool to assess the combined toxicity of herbicides in soil to rotational crops.

Changes in the persistence of two phenylurea herbicides in two Mediterranean soils under irrigation with low- and high-quality water: A laboratory approach

The disappearance of two phenylurea herbicides, chlorotoluron (CHL) and isoproturon (IPU), in two Mediterranean soils, an agricultural calcareous soil (S5) and an organic forest soil (S2), was assessed under irrigation with high- and low-quality water. Irrigation with wastewater, as opposed to irrigation with high-quality water, increased the degradation rate of both herbicides in both soils. For each soil, the decay rate of IPU was always higher than that of CHL, and both pesticides disappeared more rapidly from S5 with lower clay and organic carbon content than from S2. The degradation rate was inversely related with pesticide sorption on soil, because increased sorption would reduce pesticide bioavailability for decomposition. In most cases the residual concentration in soil of both phenylurea herbicides was better fitted to a bi-exponential decay model than to first-order or first-order with plateau models. Dehydrogenase activity, used as an indication of microbial activity, was very high in S2 in comparison with S5, but was not related to pesticide disappearance.

Урожайність сої залежно від попередника, метеорологічних умов та ефективності гербіциду

Наведені результати чотирирічних дослідженьщодо впливу попередників, зокрема пшениці озимої іячменю ярого та ґрунтового гербіциду Харнес наврожайність сої в роки з різним рівнем вологозабез-печення. Встановлено, що ґрунтовий гербіцид Харнесконтролював загальну кількість і сиру масу бур’янівза попередником пшениця озима відповідно на 92 і 93 %,а за попередником ячмінь ярий – на 91 і 83 %. Виявлено,що після попередника пшениця озима формуваласьбільша врожайність сої, ніж після попередникаячмінь ярий.&#x0D; The results of four years research of the influence of predecessors, including winter wheat and spring barley and soil herbicide Harnes on productivity soybean in years with different levels of moisture providing are presented. It is installed, that soil herbicide Harnes controlled the total wet weight number of weeds after the winter wheat predecessor respectively 92 and 93 %, and after predecessor of spring barley – 91 and 83 %. We found that after winter wheat predecessor yield of soybean was formed more than after spring barley.

Role of Alumina and Montmorillonite in Changing the Sorption of Herbicides to Biochars.

The influence of biochars on the fate of herbicides in soil depends mostly on environmental factors among which the role of soil minerals is not clear. Two wood-derived biochars produced at 400 °C (BC400) and 600 °C (BC600) were treated with alumina and montmorillonite to investigate their interaction with biochars and the influence of herbicide sorption. Both minerals exhibited a pore-expanding effect that was likely relative to the removal of authigenic organic matter away from the biochars' surface. Alumina brought more remarkable pore expansion by doubling the surface area of the BC400 biochar and the mesopore area of the BC600 biochar. Consequently, more adsorption sites were accessible for herbicide molecules, which resulted in higher sorption of herbicides (acetochlor and metribuzin) to the mineral-treated biochars than to the untreated biochars. The results are useful for understanding the change of surface and sorption properties of biochars with soil applications.

Method validation and dissipation kinetics of four herbicides in maize and soil using QuEChERS sample preparation and liquid chromatography tandem mass spectrometry

A versatile liquid chromatography tandem mass spectrometry method with modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation was developed for the determination of rimsulfuron, mesotrione, fluroxypyr-meptyl, and fluroxypyr. By adjusting the amount of graphitized carbon black, the herbicide analytes could be quantified with satisfactory recoveries in the range of 80–110%. A dissipation kinetics study conducted under open field conditions at two sites during 2014 showed first order equations with half-lives between 0.6d and 3.6d, illustrating an appropriate degree of stability and safety. The dissipation kinetics were different in the different matrices. Although the herbicides had higher initial residues in straw than those in soil, they degraded faster in straw. The terminal residues for the herbicides formulated in two water dispersible granules were all below maximum residue limits. These results not only gave insights about the analytes but also contributed to environmental protection and food safety.

Efficacy and environmental fate of imazapyr from directed helicopter applications targeting Tamarix species infestations in Colorado.

Aerial imazapyr applications are the most common and cost-effective method for controlling invasive tamarisk, but few studies have investigated whether or how infestation and site characteristics influence control and non-target impacts. This study used vertical stands with filter papers, plus soil and tree canopy sampling, to investigate how tamarisk canopies affected retention of applied imazapyr, soil herbicide residues and tree mortality. Tamarisk canopies captured 71% of aerially applied imazapyr, resulting in significantly lower soil residues beneath the tree canopy. Although initial imazapyr soil residue levels outside the tree canopy were 4 times greater than those inside, soil degradation occurred 2.4 times faster outside the tamarisk canopy and resulted in lower herbicide residues. Tamarisk mortality within 3 years was 70%, but variability in control appeared to be affected by non-linear stand boundaries and tall site obstructions. These same factors also increased variability in the actual quantity of herbicide applied, exacerbating collateral impacts on desirable understory species. While aerial imazapyr applications are highly effective in controlling tamarisk, our study provides evidence for the importance of evaluating overall site suitability for this management strategy so the probability of unintended ecological effects can be minimized.

Multi-Residue Analysis of Herbicides in Soil with an UPLC-ESI-MS Method

A rapid and sensitive method was developed for the determination of 51 herbicides in soil by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC–ESI–MS). Using acetonitrile effectively extracted 22 kinds of triazine and other basic herbicides, and using 90:10 v/v acetonitrile-phosphate buffer (pH = 7.5) effectively extracted another 29 herbicides. The extract has not cleaned up further. Chromatographic separation was achieved within 10 min using gradient elution with acetonitrile–water as a mobile phase for 22 triazine and phenylurea herbicides, and with 5 mM ammonium acetate containing 0.1% formic acid aqueous solution–acetonitrile as a mobile phase for another 29 herbicides. The response was linear over two orders of magnitude with correlation coefficients (r2) higher than 0.99. The limits of quantification for the herbicides varied from 0.2 to 20 µg kg−1. The intra- and inter-day precisions (relative standard deviation, RSD) were 2.2–9.3% and 5.7–17.1%, respectively. The average recovery varied from 61.6 to 112% with the RSD of 1.6–11.3%. Analyzing 51 soil samples from 17 counties formed the basis of this method. Three herbicide residues were found in four counties. Atrazine residue in soil for 17 counties was found; its content was 0.4–9.8 μg kg−1. Nicosulfuron residue in soil for two counties was found, with a high up to 133 or 1317 μg kg−1. Propazine (0.3 and 1.34 μg kg−1), atratone (2.14 and 3.93 μg kg−1), and cynanazine (0.34 μg kg−1) in soils for some counties were also found. The validated method can ensure the rapid multi-class, multi-residue analysis at low μg kg−1 level for 47 herbicides in soil. The developed method provides an effective analytical basis for controlling herbicide dosage, investigating their distribution and degradation, and evaluating their hazards on the environment and human health.

Rapid photodegradation of clethodim and sethoxydim herbicides in soil and plant surface model systems

Photolysis is an important degradation process to consider when evaluating the persistence of a pesticide in the field. In this work, photolytic behavior of clethodim and sethoxydim herbicides under simulated solar radiation was examined in organic solvents, on glass disk and silica gel plates as leaf and soil model surfaces. The photodegradation was characterized by determination of their half-lives (t1/2), dissipation rate constant (k) and identification of degradation products by means of HPLC-Qtof-MS. Photolytic degradation of clethodim and sethoxydim was very rapid. The photodegradation rate was enhanced in leaf model than in water with half-lives that ranged from 6.3±0.5 to 10.1±0.4min for clethodim and from 8.0±0.3 to 20.5±0.5min for sethoxydim. The fastest rate of degradation was obtained on silica gel plates with half-lives of 1.8 and 5.0min for clethodim and sethoxydim respectively. Photoreduction of the oxime ether moiety was the main transformation processes giving rise to a photostable product, the corresponding dealkoxylated derivative. Isomerization of oxime ether bond and oxidation of sulfur atom to form Z-isomer and the corresponding sulfoxides were the others reactions involved. The different environments tested influenced the concentrations of photoproducts formed during the irradiation of both herbicides. This result suggests that photolysis will be an important pathway of dissipation of the two herbicides. On the basis of these findings, further studies could be desirable to estimate the effects of transformation products on the environment.

The two paralogue phoN (phosphinothricin acetyl transferase) genes of Pseudomonas putida encode functionally different proteins.

Phosphinothricin (PPT) is a non-specific inhibitor of glutamine synthetase that has been employed as herbicide for selection of transgenic plants expressing cognate resistance genes. While the soil bacterium Pseudomonas putida KT2440 has been generally considered PPT-sensitive, inspection of its genome sequence reveals the presence of two highly similar open reading frames (PP_1924 and PP_4846) encoding acetylases with a potential to cause tolerance to the herbicide. To explore this possibility, each of these genes (named phoN1 and phoN2) was separately cloned and their activities examined in vivo and in vitro. Genetic and biochemical evidence indicated that phoN1 encodes a bona fide PPT-acetyl transferase, the expression of which suffices to make P. putida tolerant to high concentrations of the herbicide. In contrast, PhoN2 does not act on PPT but displays instead activity against methionine sulfoximine (MetSox), another glutamine synthetase inhibitor. When the geometry of the substrate-binding site of PhoN1 was grafted with the equivalent residues of the predicted PhoN2 structure, the resulting protein increased significantly MetSox resistance of the expression host concomitantly with the loss of activity on PPT. These observations uncover intricate biochemical and genetic interactions among soil microorganisms and how they can be perturbed by exposure to generic herbicides in soil.

Simultaneous determination of three herbicides in wheat, wheat straw, and soil using a quick, easy, cheap, effective, rugged, and safe method with ultra high performance liquid chromatography and tandem mass spectrometry

In this study, a sensitive and effective analytical method for the extraction and detection of three herbicide residues (florasulam, fluroxypyr, and halauxifen-methyl) in wheat and soil was developed. Samples were extracted with acetonitrile/water followed by salting out, dispersive solid-phase extraction cleanup, and detection using ultra high performance liquid chromatography coupled with tandem mass spectrometry. The target analytes were detected within a 5 min runtime using an ultra high performance liquid chromatography high-strength silica trifunctional column connected to an electrospray ionization source in positive mode. The method was validated in five replicates at three fortification concentrations in each matrix. Adequate pesticide quantification and identity confirmation were attained, even at the lowest concentration levels. The method showed very good accuracy and precision. Good recoveries were observed for the three herbicides and mostly ranged between 75.8 and 114.6%, with intraday relative standard deviations <6.01% and interday relative standard deviations <4.02%. The limits of quantification ranged between 0.14 and 7.68 μg/kg for each herbicide. The method was successfully applied for the simultaneous analysis of the three herbicides in actual trial samples, and the results proved that the proposed method was effective in detecting these three herbicides.

Sorption, desorption and leaching potential of sulfonylurea herbicides in Argentinean soils

The sulfonylurea (SUs) herbicides are used to control broadleaf weeds and some grasses in a variety of crops. They have become popular because of their low application rates, low mammalian toxicity and an outstanding herbicidal activity. Sorption is a major process influencing the fate of pesticides in soil. The objective of this study was to characterize sorption–desorption of four sulfonylurea herbicides: metsulfuron-methyl (methyl 2-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoylsulfamoyl)]benzoate), sulfometuron-methyl (methyl 2-[(4,6-dimethylpyrimidin-2-yl)carbamoylsulfamoyl]benzoate), rimsulfuron (1-(4,6-dimethoxypyrimidin-2-yl)-3-(3-ethylsulfonyl-2-pyridylsulfonyl)urea) and nicosulfuron (2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoyl]-N,N-dimethylnicotinamide) from different soil horizons of different landscape positions. Sorption was studied in the laboratory by batch equilibration method. Sorption coefficients (Kd-SE) showed that rimsulfuron (Kd-SE = 1.18 to 2.08 L kg−1) and nicosulfuron (Kd-SE = 0.02 to 0.47 L kg−1) were more highly sorbed than metsulfuron-methyl (Kd-SE = 0.00 to 0.05 L kg−1) and sulfometuron-methyl (Kd-SE = 0.00 to 0.05 L kg−1). Sorption coefficients (Kd-SE) were correlated with pH and organic carbon content. All four herbicides exhibited desorption hysteresis where the desorption coefficients (Kd-D) > Kd-SE. To estimate the leaching potential, Koc and ground-water ubiquity score (GUS) were used to calculate the half-life (t1/2) required to be classified as “leacher” or “nonleacher”. According to the results, rimsulfuron and nicosulfuron herbicides would be classified as leachers, but factors such as landscape position, soil depth and the rate of decomposition in surface and subsurface soils could change the classification. In contrast, these factors do not affect classification of sulfometuron-methyl and metsulfuron-methyl; they would rank as leachers.

Pesticide residues in vineyard soils from Spain: Spatial and temporal distributions

Spatial and temporal evaluations of seventeen pesticides and some of their degradation products were carried out in seventeen vineyard soils from La Rioja region (Spain). The soils were sampled in March, June and October 2012, and the pesticides were selected among those previously detected in surface and ground waters from the same area. All pesticides were detected in some of the soils in the three different areas of La Rioja at the different sampling times, with only the metalaxyl metabolite, CGA-62826, not being detected in any of the soils sampled in October. The highest concentrations were determined for the fungicides metalaxyl (11.5μgkg−1) and triadimenol (26.1μgkg−1), the herbicides fluometuron (174.6μgkg−1) and terbuthylazine (403.3μgkg−1), and the insecticide methoxyfenozide (4.61μgkg−1). While the highest total concentration of pesticides was detected in March, the highest number of positive detections was recorded in June (46), as opposed to 26 and 19 in March and October, respectively. Significant differences were detected in the concentrations of herbicides in soils from the three areas in La Rioja, but this was not the case for the fungicides and the insecticides. The study revealed a more intensive use of herbicides in March, while the use of insecticides and fungicides probably depended on the specific needs of crops and/or the onset of diseases. The results are consistent with the residues found in waters in the region, and highlight the need to implement strategies for more efficient application of these compounds to avoid risk of water contamination.

ADSORPTION BEHAVIOUR OF HAZARDOUS ORGANIC SOLUTIONS ONTO NEW POLYMERIC SORBENT

The fate of herbicides in soil, and surface and groundwaters is determined by several factors including retention, transformation, and transport processes. Environmental pollutants removed from waters of these chemicals is important for all biologic life. In this study, adsorption of textile dyestuffs or adsorption of pesticides such as bisphenol A, 4-amino nitrophenol, phenoxyacetic acid were examined on PVBCIm. This polymer was obtained by modifying polyvinyl benzyl chloride (PVBC) resin with imidazole. This method, has been extended to chloro methyl group to preparepoly (N-vinyl imidazole) (PVIm) graft chains on crosslinked polyvinyl benzyl chloride (PVBC) polymer. In the aqueous solution the adsorption of organic waste was determined by UV-vis spectrophotometer. For different concentrations of hazardous organic wastes in aqueous solution, Freundlich, Langmuir, and BET isotherms were applied at 25 oC.