Herbarium Material Research Articles

On the identity of the Teyde dog-rose (Rosaceae): evidence for a new endemic taxon from Tenerife, Spain

In view of the high diversity found in many groups of vascular plants on the Canary Islands, Spain, the genus Rosa (Rosaceae) is poorly represented comprising only two native taxa (R. canina, R. rubiginosa). For the first time, this study provides detailed data on distribution, morphology, chorology and status of the so-called ‘Teyde dog-rose’, a representative of the Section Caninae, which occurs exclusively in the subalpine zone of the Cañadas del Teide, Tenerife. A survey of both native stock and herbarium material yielded that this population, currently classified as common dog-rose (Rosa canina L.), is phenotypically close to continental representatives of the glaucous dog-rose (Rosa dumalis Bechst.), from which, however, it differs in several traits such as floral characters and leaf morphology. Consequently, the population is formally recognized as a distinct taxon, Rosa dumalis ssp. nov. teydensis Weller & H. Reichert. The new subspecies appears to be extremely rare in the wild, with a low number of individuals growing at scattered localities mainly at shady rocky walls and adjacent habitats in a restricted area of the southern Cañadas. Consequently, according to IUCN criteria the taxon must be regarded as Critically Endangered.

Mitthyridium wallisii (Müll.Hal.) H.Rob.: lectotypification and interrelationship with M. crassum (Broth.) H.Rob.

ABSTRACT Introduction. Syrrhopodon wallisii Müll.Hal. [≡ Mitthyridium wallisii (Müll.Hal.) H.Rob.], whose primary type material in B was destroyed during the Second World War, is lectotypified from among surviving original material, and its controversial interrelationship with its close relative M. crassum (Broth.) H.Rob. is investigated. Methods. Nomenclatural and taxonomic investigation was carried out with reference to bryological literature and herbarium material, including type specimens. It involved the microscopic investigation and analysis of leaf morphology in both historical and recent collections. Key results and conclusions. Mitthyridium wallisii is lectotypified with a specimen in the herbarium of E. Hampe (BM000662478). Based on morphological evidence, the Malesian moss taxa M. wallisii and M. crassum are likely to be distinct entities with the status of species.

Palynomorphometry in ferns: Ctenitis (Dryopteridaceae), a case of study in the Southern Cone of America

Abstract The morphometric knowledge about the spores of the Ctenitis has not yet been deepened. Considering the folded spores of this genus which inhabit the Southern Cone of America the aims were to increase infrageneric knowledge, to evaluate the relationship among the morphometric characters of the folded spores in these Ctenitis species and to verify the spore’s morphometry as a taxonomic tool. This study was performed with herbarium material. The spores were analyzed with light microscopy (LM) and scanning electron microscopy (SEM). The variables analyzed were: major and minor equatorial diameter, polar diameter, length of the laesura and length, width and height of the folds. The test chosen to analyze the study variables was ANOVA. Four spore sets are proposed. The “elongate-type” is made up of C. aspidioides and C. nervata. The “medium-type” is made up of C. anniesii, C. distans, C. falciculata and C. paranaensis. The “handle shape-type” is represented by C. bigarellae, C. eriocaulis and C. submarginalis. Finally, the “short-type”, consists only of C. deflexa. The morphometric data of spores together with the statistical analysis provided useful information for the distinction of some Ctenitis species studied in the Southern Cone of America. These results contribute to the studies aeropalynologycal and palaeopalynological.

Herbarium Pomeranicum jako przykład cyfryzacji w środowisku naukowym

Purpose – Presentation of the solutions adopted in the field of sharing the integrated digital resources of Pomeranian herbariums so far collected in four herbaria at three universities in Pomerania as a tool for commercializing scientific knowledge and presenting the role of classic and digital collections of individual herbariums in the present day and the interdisciplinary nature of the Herbarium Pomeranicum project. Research method – A single case study Results – Thanks to modern ICT technologies, herbarium material can be widely used by a number of stakeholders and play an important role not only in scientific research or didactic process, but also in assessing the degree of threat to species during the development of biodiversity conservation programs in a sustainable economy based on knowledge. Originality / value / implications / recommendations – Presenting the role of classic and digital collections gathered in the collections of individual herbariums and stakeholders in the digitization of scientific collections, including the interdisciplinary nature of the Herbarium Pomeranicum project.

Новинки в лихенофлоре заповедника «Витимский» (Восточная Сибирь)

As a result of lichenological studies in the vicinity of Lake Oron (Vitimsky State Nature Reserve) and revision of herbarium material, 9 species of lichens were identified that had not previously been listed for a specially protected natural area. Some of the identified species have a wide distribution (Absconditella lignicola, Bryoria americana, Pyxine meissneriana, Trapeliopsis flexuosa and Multiclavula vernalis), and some have limited ranges covering only the eastern part of Asia, Alaska and Southern Siberia (“Cetraria” annae, Parmelina yalungana and Phaeophyscia dessecta), with “Cetraria” annae and Phaeophyscia dessecta so far they are known only for the territory of Russia. Some of these species (Absconditella lignicola, Trapeliopsis flexuosa and Multiclavula vernalis) probably have a wider distribution in the reserve, but without spore-bearing structures they are difficult to identify. Herbarium material attributed to Parmelina yalungana does not raise doubts regarding its species identity, however, the discovery of specimens with a macular upper surface in eastern Eurasia requires additional careful study.

Ревизия лишайников рода Micarea (Pilocarpaceae) в Беларуси, с ключом для определения видов

As a result of the revision of the herbarium material stored at the V. F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus (MSK), 17 lichen species from the genus Micarea have been identified for Belarus, of which six are reported for the first time for the republic: M. byssacea, M. microareolata, M. nowakii, M. pusilla, M. pseudomicrococca, and M. soralifera. In addition, four other species are known for Belarus according to the literature. In general, the paper provides a list of 21 Micarea species. The distinguishing characters, differences from similar species, distribution in Belarus, as well as phytocoenotic and substrate confinement are discussed for each of them. A key for identification of Micarea species in Belarus is given.

Etnobotánica de los criollos del Chaco Subhúmedo argentino I: Correspondencias botánico-fitonímicas y caracterización general de un nuevo complejo biocultural

The general aim of this work is to distinguish and identify the Creole of the Argentine Subhumid Chaco as a new bio-cultural complex, to describe the botanical correspondences of their phytonymy, and to carry out a general characterization of their ethnobotany. Six field works were carried out between October 2017 and March 2022 in the center-north of Chaco Province. Classic ethnobotanical methodology was applied. This consisted of interviews and the collection of herbarium material with 51 collaborators from the area. A total of 541 matches between 369 botanical taxa and 457 types of vernacular names were obtained from 2075 reports; 1625 Criollos’ plant uses or meanings were registered. This refers to a total of 420 taxa throughout 4290 reports or mentions. Results from quantitative comparisons with Criollos’ phytonymic correspondences registered in neighboring areas of the Subhumid Chaco allow us to infer that the latter belong to the same bio-cultural complex proposed here. If we added these phytonyms to those obtained in the field it makes a total of 837 correspondences among 662 types of vernacular names assigned to 537 plant taxa, and three mushrooms. On the other hand, results of the quantitative comparisons between this phytonymy and those found in ethnobotanical investigations with Criollos from the Humid and Semiarid Chaco allow us to infer substantial differences between them, which reaffirms the condition of the Criollos of the Subhumid Chaco as a new bio-cultural complex.

New records for different lineages of Melastomataceae in the Brazilian Amazon

ABSTRACT We present novelties on the occurrence of species belonging to the tribes Marcetieae, Melastomateae and Pyxidantheae (Melastomataceae) derived from revision of herbarium material and recent collections in the Brazilian Amazon. We report the rediscovery of the type species of Comolia, known only from the original material, the first records of Appendicularia, Dicrananthera and Blakea in the states of Pará, Roraima and Rondônia, respectively, and the first record of Pterogastra in Brazil. We provide maps with the updated geographical distribution for each species based on the revision of herbarium collections available in online databases followed by manual data cleaning. Geographic location coordinates were obtained after reviewing the identities and locality information of all herbarium specimens. Most distribution records result from recent botanical expeditions within protected areas in the Brazilian Amazon. The first records of Pterogastra in Brazil expand the diversity of Melastomataceae in Brazilian flora to 59 genera. These novelties reinforce the importance of botanical expeditions throughout the Brazilian Amazon to cover the extensive spatial gaps in taxonomic knowledge in the region which should be prioritized as the focus of botanical fieldwork.

Several records of alien species new to the flora of the United Arab Emirates (UAE)

The article presents new records for five alien species previously unknown from the flora of United Arab Emirates (UAE): Euphorbia cyathophora Murr. (Euphorbiaceae), Samolus valerandi L. (Myrsinaceae), Oldenlandia umbellata L. (Rubiaceae), Cardiospermum halicacabum L. (Sapindaceae), and Corchorus aestuans L. (Malvaceae s. l. / Tiliaceae). All of them have been recorded for the first time from the emirate of Fujairah in eastern part of the UAE. Euphorbia cyathophora, which is rare in the country, was found only in “Green Oasis Nursery” at Al Dibba town, Oldenlandia umbellata– found at the beginning of the seefront at Fujairah City, and they are new for the flora of the Arabian Peninsula. Samolus valerandi was found only in the “Desert Nurseries Group store 1 (palms)” at village Al Bidiya, Corchorus aestuans – in private nursery at Al Dibba town, and Cardiospermum halicacabum was found in the Masafi friday market, they are new for the flora of Fujairah and UAE.
 Accepted names, synonyms, spatial distribution, habitat preferences, and species taxonomy with remarks on identification and differentiation from the most similar taxa occurring in the study area, as well as the list of localities are presented. The herbarium materials were transferred to the Herbarium of the Komarov Botanical Institute (LE, Saint-Petersburg, Russia), the duplicates – to the Herbarium of Altai State University (ALTB, Barnaul, Russia) and the Scientific Herbarium of Fujairah (FSH, Wadi Wuraya national park, Fujairah, United Arab Emirates).

On two species of Dracocephalum L. (Lamiaceae)

A composition of taxa included in Dracocephalum imberbe Bunge s. l. is discussed. Species rank of D. laniflorum Rupr. is reinstated based on the following characters: underground part is represented by a system of branching rhizomes, bracts absent, calyx 12–14 mm long, with triangular or narrowly triangular, pointless teeth, the middle tooth of the upper lip up to 1.5 times wider than the lateral ones and plant being nearly scentless. In contrast, in D. imberbeunderground part is represented by the taproot and developed branches of the caudex, bracts present, with well-developed non-rigid points, calyx 15–18 mm long, with lanceolate or narrowly triangular pointed (though not rigid) teeth, the middle tooth of the upper lip 1.5–2 times wider than the lateral ones, the presence of a smell that persists in herbarium specimens. Dracocephalum laniflorum is firstly reported for flora of Kazakhstan (Almaty Region) and China(Xinjiang Uygur Autonomous Region) based on the study of herbarium materials stored in AA and LE, as well as scanned herbarium specimens stored in MW, HNWP and PE, which were available through the Moscow Digital Herbarium and Chinese Virtual Herbarium (CVH) systems.

THE BUCF HERBARIUM DATABASE OF ALLOCHTHONOUS OAKS

The BUCF Herbarium stores 253 specimens of allochthonous oaks species belonging to 33 accepted taxa. Amongst the representatives, Quercus rubra has the most recordings. Moreover, three species classed as near threatened on the IUCN Red List were identified. The most significant contribution was made by the botanist S. Pașcovschi who amassed approximately one-third of the collection. The specimens were collected between 1872 and 1987, with a maximum activity between 1940 and 1949. The majority of the specimens were sampled from dendrological parks and botanical gardens in Romania, the Bazos Dendrological Park remarking itself with one of the most diverse collections of cultivated oaks. A considerable number of specimens were received from donations and exchanges with several herbaria. Most of the herbarium material is very well-preserved having high potential for future investigations.

A Taxonomic Revision of Chamaecrista (Caesalpinioideae, Cassieae, Cassiinae) in Southern Africa

Abstract— The southern African species of Chamaecrista were all treated under Cassia in Gordon-Gray’s treatment for the Flora of Southern Africa. However, given the subsequent generic recircumscriptions in the subtribe Cassiinae, and the expanded collections of these taxa, there is a need to revisit the taxonomy of the group. The present study aimed to conduct a detailed taxonomic revision of the species of Chamaecrista indigenous to the flora of southern Africa region. Extensive fieldwork was carried out to study the taxa in their natural environment and morphological characters were additionally studied using herbarium material. The revision presented here includes comprehensive descriptions, a key to the species, nomenclature, typifications, diagnostic characters with illustrations, and geographical distribution maps of all recognised taxa. Eleven species of Chamaecrista are recognised for the flora of southern Africa, two of which are described as new, i.e. Chamaecrista grandiglandulata and C. gordon-grayei. In addition, one new subspecies is described, namely C. gordon-grayei subsp. longipedicellata, while Chamaecrista comosa var. capriconia is raised to the rank of subspecies, viz. Chamaecrista comosa subsp. capriconia. The typifications published here include lectotypes designated for Chamaecrista plumosa and C. stricta, neotypes designated for C. capensis var. flavescens, C. comosa, and C. plumosa var. diffusa, and an isolectotype designated for C. stricta.

Revision of the genus Piptatherum P. Beauv. (Poaceae) in Bosnia and Herzegovina

The paper reviews the results of detailed revision of herbarium material of the genus Piptatherum from Poaceae collection kept in the Herbarium of the National Museum of Bosnia and Herzegovina (SARA). Revision of the genus Piptatherum P. Beauv. in Bosnia and Herzegovina is presented, with brief nomenclature citations and the key for identification. The original photographs and distributional map, accompany the synopsis for each taxon: Piptatherum holciforme (M. Bieb.) Roem. & Schult. subsp. holciforme, P. miliaceum (L.) Coss. subsp. miliaceum, P. miliaceum subsp. thomasii (Duby) Freitag and P. virescens (Trin.) Boiss.

Digitisation of the Natural History Museum's collection of Dalbergia, Pterocarpus and the subtribe Phaseolinae (Fabaceae, Faboideae).

In 2018, the Natural History Museum (NHMUK, herbarium code: BM) undertook a pilot digitisation project together with the Royal Botanic Gardens Kew (project Lead) and the Royal Botanic Garden Edinburgh to collectively digitise non-type herbarium material of the subtribe Phaseolinae and the genera Dalbergia L.f. and Pterocarpus Jacq. (rosewoods and padauk), all from the economically important family of legumes (Leguminosae or Fabaceae).These taxonomic groups were chosen to provide specimen data for two potential use cases: 1) to support the development of dry beans as a sustainable and resilient crop; 2) to aid conservation and sustainable use of rosewoods and padauk. Collectively, these use case studies support the aims of the UK's Department for Environment Food & Rural Affairs (DEFRA)-allocated, Official Development Assistance (ODA) funding. We present the images and metadata for 11,222 NHMUK specimens. The metadata includes label transcription and georeferencing, along with summary data on geographic, taxonomic, collector and temporal coverage. We also provide timings and the methodology for our transcription and georeferencing protocols. Approximately 35% of specimens digitised were collected in ODA-listed countries, in tropical Africa, but also in South East Asia and South America.

A long-flowering Hechtia (Bromeliaceae): Hechtia sanchezii, a new species from San Luis Potosí, Mexico

Background: Hechtia is known among Bromeliaceae by its unisexual, sexually dimorphic, mainly fragrant flowers and dioecy; delimitation of taxa particularly on those with lateral inflorescence, requires qualitative morphological evidence from both sexes, because dimensions of structures change during its growth.
 Hypotheses: Hechtia species are delimitated based on a combination of discrete morphological characters from both sexes, associated with narrow geographical distributions. If examination of populations of San Luis Potosí shows characters that place the taxon in the genus and distinguish it from other species in Hechtia, the taxon will be described as new.
 Taxon: Hechtioideae, Hechtia sp. nov.
 Study site and dates: Mexican State of San Luis Potosí and vicinities, 2015-2021.
 Methods: Live plants as well as herbarium material of the new taxon and the most morphological similar species, were analyzed, with examination of inflorescences and flowers of both sexes, fruits, and seeds. Geographical distribution was mapped and conservation status using IUCN criteria is reported for the new species.
 Results: Hechtia sanchezii has been confused with H. glomerata since both share lateral, glomerulate-like infructescences, but H. sanchezii shows red to ferruginous indumenta on floral bracts and sepals (vs. white), these red (vs. greenish or brownish), pink petals (vs. white), scentless (vs. fragrant) flowers, stamens with filaments of two lengths, three epipetalous, and three alternating with petals (vs. two lengths but all filaments epipetalous).
 Conclusions: Morphological and geographical evidence allow the recognition of H. sanchezii as a new species native from San Luis Potosí, Mexico.

Результаты флористического мониторинга в национальном парке «Орловское полесье» в 2022 году

The report describes the results of floristic monitoring realized by the authors in 2022 on the territory of the Znamensky and Khotynetsky Districts of the Oryol Region within the boundaries of the Orlovskoye Polesye National Park. For the park, 7 new species of vascular plants were noted, of which 3 are reported for the first time for the flora of the Oryol Region, and the growth of 1 species was confirmed 120 years after the discovery in the specified territory. During the research, as far as possible, the number of individuals in the coenopopulation was recorded, morphometric indicators, the occupied area were determined, and information on the dynamics of the coenopopulation size was collected. Herbarium materials confirming the records were transferred to the Herbarium of the Oryol State University named after V. N. Khitrovo (OHHI).

Red-listed plants, lichens and fungi of the Teriberka Nature Park and its surroundings, Murmansk Region

The article presents a brief background of the botanical study of the Teriberka Village environs and the studies for substantiating the establishment of a protected area - Teriberka Nature Park. The information on the locations of rare and protected species of plants, fungi and lichens in the Teriberka Nature Park and its surroundings was summarized based on the analysis of herbarium and archival materials, literature sources and fieldwork in 2020. Overall, 22 species of plants, lichens and fungi from the Red Data Book of the Murmansk Region and four species from the Red Data Book of the Russian Federation were identified inside the park and in its immediate vicinities. There are 13 regionally red-listed species within the boundaries of the Teriberka Nature Park, two of them listed also in the Red Data Book of the Russian Federation. The most valuable habitats in terms of protecting rare plants, lichens and fungi were identified.

Conspectus of the genus <i>Juniperus</i> L. growing in Kazakhstan

Background. The genus Juniperus L. is the most numerous in the family Cupressaceae Bartl. and includes about 75 species. There are from 7 to 10 species of this genus in Kazakhstan. All types of juniper are widely used in folk medicine and play an important ecological role. The purpose of our work was to study the current status, systematics and distribution of juniper species. This will help to clarify and expand the information on the current location of Juniperus species in Kazakhstan and will be used in the future to study intra- and interspecific genetic diversity of the genus.Materials and methods. To study the distribution and make a conspectus of the Juniperus spp. growing in Kazakhstan, floristic reports, materials of plant explorations and the herbarium collections for the period from 1890 to 2021 obtained from the herbarium of the Institute of Botany and Phytointroduction (AA), herbarium materials of the Institute of Plant Biology and Biotechnology as well as the digital herbarium of the Lomonosov Moscow State University – Depository of Living Systems “Noah’s Ark” (MW) were analyzed.Results. As a result of the study, 630 herbarium sheets were processed and 70 locations of 7 juniper species growing in Kazakhstan belonging to 2 sections – Juniperus (=Oxycedrus Endl.) and Sabina Endl. – were identified. A conspectus of 7 juniper species identified during the analysis of the three above-mentioned herbarium collections was made. The main division of species is observed in the context of administrative and floristic regions of Kazakhstan. The greatest species concentration occurs in Southern, South-Eastern and Eastern Kazakhstan. The results of the study contribute to the inventory and search for previously forgotten places of growth for the genus Juniperus. Besides, the continuation of comprehensive botanical and molecular genetic studies on junipers will help to examine and preserve the genetic diversity of the species and conserve forest bioresources.

Notes on the distribution of Ligularia kingiana (W. W. Sm.) Hand.-Mazz. (Asteraceae): new records

The Himalayan species Ligularia kingiana (W. W. Sm.) Hand.-Mazz., which for a long time was considered as endemic to the state of Sikkim (India), was first found during field work in the state of Nagaland, almost 700 km from the type locality. The study of herbarium materials and literature revealed that this rare species also occurs in eastern Nepal (Solukhumbu District) and northern Myanmar (Kachin State). For Nepal, L. kingiana is reported for the first time.

Cellulose in Foliage and Changes during Seasonal Leaf Development of Broadleaf and Conifer Species.

Stable isotope approaches are widely applied in plant science and many improvements made in the field focus on the analysis of specific components of plant tissues. Although technical developments have been very beneficial, sample collection and preparation are still very time and labor-consuming. The main objective of this study was to create a qualitative dataset of alpha-cellulose content of leaf tissues of arboreal species. We extracted alpha-cellulose from twelve species: Abies alba Mill., Acer pseudoplatanus L., Fagus sylvatica L., Larix decidua Mill., Picea abies (L.) Karst., Pinus sylvestris L., Quercus cerris L., Quercus petrea (Matt.) Liebl., Quercus pubescens Wild., Quercus robur L., Tilia platyphyllos Scop. and Ulmus glabra Huds. While these species show an increase in cellulose yield from bud break to full leaf development, the rates of increase in cellulose content and the duration of the juvenile phase vary greatly. Moreover, the veins display significantly higher alpha-cellulose content (4 to 11%) compared to blade tissues, which reflects their different structural and biochemical functions. A guide for the mass of sample material required to yield sufficient alpha-cellulose for a standard stable isotope analysis is presented. The additional benefits of the assessment of the mass of required sample material are reduced sample preparation time and its usefulness in preparing samples of limited availability (e.g., herbarium material, fossil samples).