Herbal Toothpaste Research Articles

Review on Formulation of Polyherbal Toothpaste Using Medicinal Plants

Toothpaste is a gel or paste formulation product and is use to clean and maintain oral hygiene with the aid of toothbrush. But some of these substances show undesirable side effects such as tooth staining and altered taste. India is that the largest producer of healthful herbs and is termed the “Botanical Garden of the world”. The objective of the present review to formulate herbal toothpaste containing natural ingredients like clove fruit, Neem leaves, Honey and Acacia powder, Betel, Peppermint, Turmeric, Guava etc. which were traditionally used for tooth cleaning. They not only show antimicrobial property for naturally action diseases however even have essential concentration of bound phytochemical present in them which will profit our body in some ways. Based on increased usage of herbal cosmetics we tried to make a comprehensive review on herbal toothpaste that helps to maintain a proper oral hygiene and free from periodontal disorder, reduce stain, gingivitis, calculus and caries. The present review gives basic information regarding antimicrobial potential of various herbs, formulation excipients, that can be used in preparation of toothpaste. Herbal toothpaste which can be used as a tool for proper oral hygiene and to overcome the side effect of the conventional toothpaste by synthetic ingredients.

Efficacy of a Eucalyptus oil-based dentifrice in reducing plaque and gingival bleeding scores - A randomized clinical crossover study.

Herbal products in dentistry have grown significantly. In the current scenario, herbal products are believed to be an effective adjunct to other medications. The present study aims to evaluate Eucalyptus oil and miswak (Salvadora persica) toothpaste for its efficacy in observable reduction in plaque and gingival bleeding. Sixty participants with gingivitis were enrolled in the present study. The study included an interim period (washout) comparing miswak and Eucalyptus toothpaste. Plaque scores were measured at designated time intervals. Both herbal toothpastes significantly decreased plaque index. Nevertheless, with relation to miswak (P = 0.002), Eucalyptus oil-based toothpaste exhibited reduction in bleeding scores. When participants were asked to return to their routine toothpaste, no changes were observed. Results from the study showed that the toothpaste containing Eucalyptus showed a significant decrease in gingival bleeding. More investigations should be looked on the medicinal applications of Eucalyptus toothpaste on commonly seen periodontal parameters.

Effectiveness of a novel experimental herbal toothpaste against bacterial consortium associated with dental caries.

To evaluate the effectiveness of an experimental toothpaste based on Hypericum laricifolium Juss. essential oil against a bacterial consortium associated with dental caries. The antibacterial activity of the essential oil was evaluated by the disk diffusion and microdilution tests against Streptococcus mutans, Streptococcus sanguinis and Streptococcus salivarius. Petri dishes were incubated at 37°C for 24 h. An experimental toothpaste was prepared at a concentration of 201.76 mg/mL. The antibacterial activity of the experimental and six commercial toothpastes were evaluated by agar well-diffusion method. Petri dishes were inoculated with a bacterial consortium prepared with the three strains. 80 mg of the toothpastes were placed in the wells and the Petri dishes were incubated at 37°C for 24 h. The inhibition zones were measured with a digital compass. The differences between the pastas were evaluated with the one-way ANOVA test, with a 5% level of significance. The essential oil was more effective than 0.12% chlorhexidine in inhibiting the growth of S. mutans (29.02±1.74 mm) and S. sanguinis (21.92±3.43 mm), being more moderate for S. salivarius (17.66±1.11 mm) . In MBC tests, the EO showed complete inhibition of the growth of S. mutans at a concentration of 5% (50.44 mg/mL), 10% (100.88 mg/mL) for S. sanguinis and 2.5% (25.22 mg/mL) for S. salivarius. The experimental toothpaste was effective in inhibiting the growth of the bacterial consortium (31.88±66 mm), having a similar performance to Total Dent, Colgate Total 12, Kolynos® Herbal and Colgate® Herbal (p> 0.05). The development of an experimental paste based on H. laricifolium Juss. essential oil (0.28% v/v) showed an important antibacterial activity similar to commercial toothpastes against a bacterial consortium of S. mutans, S. sanguinis and S. salivarius. Key words:Antibacterial agents, toothpastes, medicinal plants, Streptococcus mutans, Streptococcus sanguinis, Streptococcus salivarius.

Antimicrobial and anti-inflammatory activities of Annona muricata L. (Annonaceae) formulated as an herbal toothpaste

Purpose: The major drawback with many orthodox conventional types of toothpaste is their side effects and sometimes resistance associated with their use. It is on this basis that we investigated the anti-inflammatory and antimicrobial activities of the leaves of Annona muricata L. (Annonaceae) formulated as herbal toothpaste. Methods: Phytochemical and chromatographic screenings were investigated using standard procedures. Anti-inflammatory evaluation was carried out using egg albumin. The antimicrobial evaluation of extract of A. muricata and formulated herbal toothpaste was by agar well diffusion method against selected microorganisms using ciprofloxacin and nystatin as positive controls. The sensory and physicochemical properties of the formulated herbal toothpaste were evaluated. Results: Phytochemical screening of A. muricata revealed the presence of tannins, cardiac glycosides and alkaloids. The anti-inflammatory activity was dose dependent. There was inhibition of activity by the crude extract and formulated herbal toothpaste containing A. muricata against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Candida albicans. The A. muricata formulated herbal toothpaste was sweet and pleasantly smelling with good foaming ability and had a pH range of 7.24-7.92. Conclusion: A. muricata possess chemical constituents which could be responsible for the observed anti-inflammatory and antimicrobial properties. These constituents when present in the formulation of toothpastes could result in more therapeutic effectiveness and reduction in resistance. Keywords: Annona muricata, anti-inflammatory, antimicrobial, toothpaste

Comparison of the Remineralization Effect of Nano Herbal Toothpaste With Two Kinds of Toothpaste Containing Fluoride and Bioactive Glass on Primary Enamel Lesions: An In Vitro Study

Background: Considering the therapeutic properties of the compounds used in Albodent Iranian toothpaste and little evidence about the effect of NovaMin toothpaste on primary caries lesions, the purpose of this research was to compare the effect of toothpaste containing fluoride and toothpaste containing bioactive glass with Albodent toothpaste on enamel remineralization in a laboratory manner. Methods: Overall, 24 healthy human premolar teeth were selected and divided into buccal and lingual halves. The demineralization cycle of the samples was performed for 14 days. The samples were classified into four groups and were brushed twice a day for 28 days with tested toothpaste. A microhardness test was conducted, and changes in the enamel surface and morphology of one sample selected from each group were evaluated by scanning electron microscopy. The analysis of variance and Tukey’s tests were used for the statistical analysis of the data. Results: The average difference in microhardness in the studied groups was statistically significant (P<0.05) when compared two-by-two with the control group. The most noticeable difference was found in the comparison of the Sensodyne group with the control group, while the lowest difference was observed in the comparison of the Albodent group with the control group. Conclusion: The presence of NovaMin in toothpaste along with fluoride can increase the efficiency of the remineralization rate of primary enamel lesions compared to fluoride toothpaste without bioactive glass. In addition, the effectiveness of herbal toothpastes, which do not contain essential artificial ingredients in the remineralization of primary enamel lesions was lower than that of non-herbal fluoride toothpastes.

The potential of rambutan honey toothpaste in reducing the dental plaque index: a quasi Experimental study

ABSTRACTIntroduction: The accumulation of dental plaque is a common cause of dental and oral health problems such as caries and periodontitis, therefore it is necessary to control plaque. Brushing is the most effective way to control plaque. Currently, there are various toothpastes used by the people, one of which is herbal toothpaste. One of the herbal toothpastes that has an antibacterial potential is rambutan honey toothpaste. The purpose of this study was to analyze the effectiveness of rambutan honey toothpaste in reducing the dental plaque index. Methods: this research was Quasi Experimental design research with a pre-test post-test control group design. The twenty samples were divided into three treatment groups, namely the group that brushed teeth with base toothpaste, rambutan honey toothpaste, and aquadest. All samples were asked to brush their teeth using base toothpaste, rambutan honey toothpaste, and aquadest twice, after using toothpaste for one day, the plaque index was measured by the Silness and Loe method. Data were analyzed using Kruskal-Wallis and post hoc Mann-Whitney tests (p<0.05). Results: There was a significant difference between the plaque index score before and after using rambutan honey toothpaste with a p value=0.005 (p≤0.05). The results also showed the effect of brushing teeth with base toothpaste, rambutan honey toothpaste, and aquadest on plaque index with a value of p=0.005 (p≤0.05). The results showed that there was no significant difference in dental plaque index between base toothpaste and rambutan honey toothpaste with a p value=0.583. Conclusion: Rambutan honey toothpaste has a potential in reducing the dental plaque index.Keywords: plaque index, rambutan honey, toothpaste

Potential of tobacco leaf extract paste (Nicotiana tabacum L.) as a denture cleanser to prevent denture stomatitis

The percentage of use of dentures is relatively high. Dentures with less than optimal cleaning can cause Candida albicans infection, called denture stomatitis. The chemical method of cleaning agents from effervescent tablets of tobacco leaf extract has been studied. However, it is still not optimal, so a combination of mechanical cleaning is needed, namely by making a paste preparation. The aim is to analyze the most effective concentration of tobacco leaf extract in acrylic resin denture cleaning paste in reducing the number of C. Albicans. Type of laboratory experimental research. There were 4 sample groups, namely A: HPAI herbal toothpaste. B: Tobacco leaf extract paste (PEDT) 25%. C: PEDT 50%. D: 75% PEDT. The number of samples for each group was 10. Making Acrylic Resin Plates size 65 mm × 11 mm × 3.5 mm, Making tobacco leaf extract paste 25%, 50%, 75%. Preparation of C. albicans Suspension. Treatment of acrylic plates. Next, calculate the amount of C. Albicans using a spectrophotometer. There was a significant difference (p<0.05) between the control group, the 25% PEDT group, and the 75% PEDT group. A non-significant difference (p>0.05) was found between the control group and the 25% PEDT group and 50 PEDT %, PEDT group 25% to 50% PEDT group, and PEDT group 50% to 75% PEDT group. Effervescent tablets of tobacco leaf extract (Nicotiana tabacum L.) are 75% effective in inhibiting C. Albicans with 30 minutes of soaking time.

Formulation and evaluation of herbal toothpaste containing eupatorium triplinervisleaf extract

Eupatorium triplinervis (Asteraceae),popularly known as Ayapana , is widely used in folk medicine, due to its antibacterial, antifungal, analgesic, antianorexic, antiparasitic, anthelmintic and sedative properties. The present study was focused on evaluating the anti-bacterial action of the formulated herbal toothpaste containing Eupatorium triplinervis leaf extract and determining the best formula for preparing the toothpaste. The extraction was conducted by the soxhlet apparatus using methanol as solvent and was subjected to preliminary phytochemical screening. The antimicrobial activity of the extracts was performed and the minimum inhibitory concentration (MIC) values of the extract were reported against three organisms- Staphylococcus aureus ( gram positive), E. coli ( gram negative) and oral flora by agar dilution assay. Also, the antibacterial activity by agar well diffusion method was determined for the prepared toothpaste against all the above three bacteria. The phytochemical screening shows the presence of coumarins in the methanolic extract which were responsible for the antibacterial activity of the extract. The formulations shows considerable zoneof inhibition when compared with a marketed toothpaste formulation (Dant kanti). Thus, this toothpaste preparation has antibacterial properties and so can be used for gingivitis and other bacterial disorders of the oral cavity.

Comparative evaluation of herbal toothpaste formulations: A preliminary study

Comparative evaluation of herbal toothpaste formulations: A preliminary study

DIFFERENCES OF HERBAL AND NON-HERBAL TOOTHPASTE AGAINST DECREASING PLAQUE INDEX OF ADOLESCENT WOMEN

Background: Women had better dental and oral health than men because women had better fine motor skills and manual dexterity than men. Dental and oral health problems commonly found in adolescents were caused by plaque. Plaque is a soft layer tightly attached to the tooth’s surface. Plaque could be cleaned mechanically and chemically by brushing teeth using toothpaste. Toothpaste was divided into two types, namely herbal and non-herbal toothpaste which had ingredients that were efficacious for dental health. Purpose: Analyzing the differences in the effectiveness of herbal and non-herbal toothpaste against decreasing plaque index in adolescent women around 11-19 years at the Aisyiah Hikmah Zam-Zam Orphanage and Harapan Ibu Orphanage, Banjarmasin. Methods: This study used the true experimental method with a pre-and post-test control group design, using simple random sampling. Result: The Wilcoxon test in the herbal toothpaste group obtained a significant value of 0.001 (<0.05) which meant that there was a significant difference between the plaque index before and after using herbal toothpaste, while in the non-herbal toothpaste group a significant value of 0.124 (>0.05) which meant that there was no significant difference between the plaque index before and after using non-herbal toothpaste. The significance of the Mann-Whitney test in the herbal and non-herbal toothpaste group was 0.037 (<0.05), which meant that there were differences in the effectiveness of herbal and non-herbal toothpaste in adolescent women. Conclusion: Toothpaste with herbal content was more effective in lowering plaque index in adolescent women compared to non-herbal toothpaste. Keywords: Adolescent, Herbal Toothpaste, Nonherbal Toothpaste, Plaque Index

Herbal Toothpaste Extracts (Betel Leaf, Lime, and Salt) to Reduce Plaque

Data from the 2018 Riskesdas reported that 96.5% of the 10-14 years old group had good tooth brushing behavior, namely brushing their teeth every day. However, from this percentage, only 2.1% brushed their teeth at the right time. Plaque cannot be removed be simply rinsing and sprying water. Plaque can only be completely removed by mechanical means. Herbal toothpaste is toothpaste that contains natural ingredients such as extracts (betel leaf, lime and salt). The addition of herbs to toothpaste is expected to inhibit plaque growth. The aim of the study was to determine the effect of herbal toothpaste with betel leaf extract, lime juice and salt to reduce plaque in grade IV children at Pudak Payung 01 Elementary School.The type of research used was descriptive quantitative with a cross-sectional approach. Namely by examining the plaque directly into the oral cavity of children who are research respondents. The results showed that the herbal extract paste (betel leaf, lime, and salt) was more effective and most significant in reducing the dental plaque score, which decreased by 22.2 with an average decrease in score of 1.2. It is hoped that children at Pudak Payung 01 Elementary School can always maintain healthy teeth and mouth by using herbal extract tooth paste (betel leaf, lime and salt).

Formulation and Evaluation of Herbal Toothpaste without Using any Harsh Chemical

Formulation and Evaluation of Herbal Toothpaste without Using any Harsh Chemical

The Biocompatibility of a Ginger-Containing Herbal Toothpaste on Developing Zebrafish Embryos

The biocompatibility of toothpaste in an oral cavity should be approved by clinical trials. Nowadays, herbal toothpaste is increasing in popularity due to its natural ingredients. Being genetically similar to humans, zebrafish are used in potential toxicity testing. The zebrafish embryotoxicity test is a fast and straightforward method to study chemical toxicity during embryogenesis. Objective: This study aimed to evaluate if there was any biocompatibility of the toothpaste on zebrafish embryos. Methods: Adult AB strain zebrafish were used according to Institutional Animal Care and Use Committee protocols. Normally dividing, spherical embryos were exposed to herbal toothpaste with ginger (Gumgumix, Turkey) (50 mg/L) and conventional toothpaste (Signal, Expert Protection, Bulgaria) (50 mg/L) in well plates containing 20 embryos, having four replicates. Developmental effects, mortality, and hatching rates were evaluated for 72h. Results: Zebrafish embryos exposed to conventional toothpaste had a higher mortality rate than those exposed to herbal toothpaste; they hatched later and delayed in development. There was no difference between herbal toothpaste and the control group regarding mortality and hatching rates (p > 0.005). Conclusion: The herbal toothpaste showed higher biocompatibility on zebrafish embryos compared to the conventional toothpaste under the condition of this study.

Formulation and Evaluation of Polyherbal Toothpaste: To Overcome Oral Problems

Abstract: Polyherbal toothpaste with natural ingredients is more acceptable in public opinion than chemical-based synthetic formulations in the current oral dental care scenario due to their safety and efficacy in reducing dental caries and preventing other dental disorders to which this generation is prone. In this composition, we use, clove, Neem, pomegranate peel , all of which have never been used before in any research. These extracts have anti-ulcer, anti-caries, anti-bacterial, and remedial properties, as well as certain unique properties including anti-cancer and anti-fungal. Herbal toothpastes were evaluated to determine important physical characteristics such as pH, stability, extrudability, spread ability, foamability, and homogeneity to develop a more effective and stable product. The novelty of this project is to overcome teeth problems to minimizing adverse effect of synthetic drug by using polyherbal ingredients.

Formulation and Evaluation of Poly Herbal Tooth Paste

Oral hygiene can be maintained throughout the day by using various dentifrices prepared with herbal and synthetic ingredients. Oral hygiene is maintained to keep the mouth fresh and avoid tooth decay. The largest producer of healthful herbs is India which is known as the botanical garden of the world. The main aim of this work is to formulate and evaluate polyherbal toothpaste and compare it with marketed products of the same category. The toothpaste was prepared by using several herbal ingredients which show antibacterial, antiseptic, and cooling properties. Neem, clove, babool, banyan, amla, and many other natural products are used to formulate ideal herbal toothpaste which satisfies all the required properties to keep the mouth fresh and to prevent tooth decay caused by the bacteria. The method which is used for formulating the herbal toothpaste is the trituration method. The prepared toothpaste was evaluated for its organoleptic and physical characteristics such as color, odor, taste, stability, foamability caused by bacteria, and abrasiveness to ensure that it possesses all the desired features to use against dental disease. Thus, the formulated herbal toothpaste was a good herbal toothpaste than the conventional toothpaste due to its side effect.

DEVELOPMENT OF TOOTHPASTE FORMULATION MODEL OF MANALAGI APPLE PEEL EXTRACT (Malus Sylvetris) WITH DIFFERENT CONCENTRATIONS AGAINST THE GROWTH OF STREPTOCOCCUS BACTERIA MUTANS IN VITRO AND IN VIVO


 
 
 
 
 
 
 
 
 
 
 
 
 Background: Caries is a disease caused by damage to the enamel layer that can extend to the nerve part of the tooth. One way to prevent caries is to brush your teeth regularly using antibacterial herbal toothpaste that can reduce the number of colonies of streptococcus mutans bacteria. Apple peel is a useful herbal ingredient as an antibacterial, antioxidant, antifungal and antiproliferative, and polyphenolic compound. Objective: To determine the effect of developing a toothpaste formulation model of manalagi apple peel extract (malus sylvetris) with different concentrations on the growth of mutant Streptococcus bacteria in vitro and in vivo. Method: This study is a true experimental research using pre test and post test approach with control group design. Sampling was carried out using a simple random sampling technique and 15 respondents were obtained. This study was carried out for 3 days, where on the first day measurements were carried out (pretest) by taking saliva samples on respondents before brushing their teeth in the morning, and on the third day (posttest) measurements of respondents' saliva were carried out to test the bacterial colonies contained therein. Statistical analysis of Pre-Test Post-Test difference test using Paired T-Test difference test and One-Way Annova Test and Comparison Analysis with Post Hoc Tukey test. Results: This study showed that the dose of toothpaste in vitro testing was obtained the optimal dose, namely at the concentration of apple peel extract manalagi 25%, then in vivo tests the toothpaste formulation model of Manalagi apple peel extract (Malus Sylvetris) with the optimal dose (25%) was effective in inhibiting the growth of streptococcus mutans bacteria in vivo with an average decrease of -3.30 x 106 CFU / ml, with p value (0.005 < 0.05). control group + was -0.38 x 106 CFU/ml, and non-herbal toothpaste in control group - was -0.68 x 106 CFU/ml, but results Statistical analysis showed no significant difference in results before and after treatment in control group + (p value = 0.553 > 0.05) and control - (p value = 0.403 > 0.05). Conclusion: Manalagi apple peel extract toothpaste formulation model (malus sylvetris) with a concentration of 25% effective in inhibiting the growth of mutant Streptococcus bacteria in vitro and in vivo
 
 
 
 
 
 
 
 
 
 
 
 

Formulation and Evaluation of Herbal Toothpaste against Biofilm Producing Staphylococcus aureus

Dental and oral hygiene is essential for overall health and well-being. Tooth brush and dental floss help remove food debris and plaque, while tooth paste and mouth wash are a key part of oral hygiene as they reduce bacterial growth. The use of chemical agents in tooth paste formulations are effective but can have undesirable side effects. The use of herbal extracts instead can be equally effective due to the presence of phytochemicals which render antibacterial and antiseptic properties, with no side effects. In this study we have formulated toothpaste using the extracts of orange peel, tomato peel and Eucalyptus leaves. The extracts were positive for phytochemicals like terpenoids, flavonoids and tannins. The formulated tooth paste demonstrated antibacterial and biofilm degrading activity against Staphylococcus aureus isolated from dental plaques. On comparison with commercially available toothpastes, the herbal formulation was observed to be more effective against the biofilm forming Staphylococcus aureus. Rakhi Bajpai3, Chitranshu Pandey4, Varaprasad Kolla*5

Evaluation of Anti-plaque and Anti-gingivitis Efficacy of Two Commercially Available Herbal and Non-herbal Toothpastes.

Plaque-associated oral disease affects a considerable portion of the population and is considered one of the major causes of tooth loss. The presence of plaque may be the reason for dental caries, gingivitis, periodontal problems, and halitosis. Many mechanical aids are used to control plaque, including toothbrushes, dental floss, mouth rinses, and dentifrices, and the most effective method of controlling gingivitis is supragingival plaque control. To evaluate and compare the anti-plaque and anti-gingivitis efficacy of commercially available herbal toothpaste (Meswak) and non-herbal toothpaste (Pepsodent). 50 subjects aged between 10 and 15 years with a full complement of dentition were included in the study. The two toothpastes were provided to the subjects in plain white tubes by the investigator. Subjects were instructed to brush their teeth twice daily using the given toothpaste for 21 days. Plaqueand gingival scoreson days 0, 7, and 21 were recorded, and the data were subjected to statistical analysis. At the end of the 21-day study, there was a statistically significant difference between the groups for plaqueand gingival scores. The plaqueand gingival scores were significantly reduced throughout the study in both groups. In comparison, the herbal dentifrices show more effectiveness in reducing plaqueand gingivalscores, but no statistically significant difference was seenbetween the two groups.

Formulation, Evaluation and Antibacterial Effect of Herbal Toothpaste using leaves and flowers Acmella ciliata

Oral hygiene, an integral part of the body’s general well-being, it is the practice of keeping the mouth clean and disease free by using tooth paste, mouth wash etc. Plants have been a great source of medical agents for many years and even be considered the origin of modern medicine. Phytochemicals the secondary metabolites of the plants are active ingredients that possess therapeutic properties that are considered as a medicine or drug for therapeutic purposes for the curing of diseases and improving human health. The main objective of this study is to formulate and develop an herbal toothpaste from herbal source and to evaluate its antibacterial activity. The herbal toothpaste containing leaves and flowers of Acmella ciliate, clove, honey and leaves of guava as the herbal ingredients. Different types of formulations (F1-F3) were formulated using Calcium carbonate as cleaning and polishing agent, Glycerine as humectant, Sodium lauryl sulphate, Sodium chloride as a stain remover from the teeth, Honey. The three formulations were evaluated by various parameters like colour, taste, fragrance, consistency, pH, shape maintenance, threading property, moisture content and foaming character. The herbal toothpaste having aqueous extract of herbal source are tested for antibacterial activity against K. pneumoniae, S. aureus, P. aeruginosa and S. pyogenes. The result shows that the bacteria were found to be more sensitive to the formulated toothpaste as seen by zone of inhibition. Further studies would benefit from a uniform method of assessment for clinical effectiveness of plaque and gingivitis and safety of the formulated herbal toothpaste. 
 Keywords: Phytochemicals,Acmella ciliate,herbal toothpast, antibacterial activity

Knowledge and Attitudes of Pregnant Women with and without Children about Fluoride and Herbal Toothpastes

Objective: Objective: Pregnant women may be vulnerable to dental caries due to their inability to fully implement oral hygiene practices. Toothpastes are main component of oral hygiene and the most important tool for the primary prevention of caries. The study aimed to examine the knowledge and attitudes of pregnant women with children (PC) and without children (PNC) about fluoride and herbal toothpastes. Methods: A self-administered and validated 20-item questionnaire was completed by a total of 219 pregnant participants, 85 PC and 134 PNC. Statistical analyses were performed using the SciPy v1.2.3. program. Results: Most of the PC (57.65%; 69.41%) and PNC (72.39%; 47.76%) participants responded with “no idea” when asked about fluoride sources and the optimal amount of fluoride added to tap water by local health authorities (p= .006). The majority of the PC (62.4%) and the PNC (47.0%) had no preference for herbal toothpastes during pregnancy (p= .03). In addition, 86.6% of the PNC showed low knowledge about the nonfluoride content of herbal toothpastes (p= .023). While 51.5% of the PNC responded with “no idea” about a preference for herbal toothpastes for their children, 56.47% of the PC stated that they might not prefer using herbal toothpastes for their children’s routine oral hygiene. Conclusion: The findings show that both PC and PNC participants had little knowledge of toothpastes and their contents. Considering that toothpastes are the most common self-applied oral hygiene tools, knowledge and awareness of fluoride and herbal toothpastes should be raised via antenatal programs.