Herbal Decoction Research Articles

Mechanisms of the Ping-wei-san plus herbal decoction against Parkinson's disease: Multiomics analyses.

Parkinson's disease is a neurodegenerative disorder involving loss of dopaminergic neurons. Multiple studies implicate the microbiota-gut-brain axis in Parkinson's disease pathophysiology. Ping-wei-san plus Herbal Decoction, a traditional Chinese medicine composition with beneficial effects in Parkinson's disease, may have a complex array of actions. Here we sought to determine whether gut microbiota and metabolic pathways are involved in Ping-wei-san plus herbal therapy for Parkinson's disease and to identify functional pathways to guide research. The model of Parkinson's disease were induced with the rotenone. The Ping-wei-san plus group received the PWP herbal decoction for 90 days, after which all groups were analyzed experimentally. PWP herbal treatment improved motor behavior and emotional performance, balanced gut microbiota, and benefited dietary metabolism. Tandem Mass Tags mass spectrometry identified many differentially expressed proteins (DEPs) in the substantia nigra and duodenum in the PWP group, and these DEPs were enriched in pathways such as those involving cAMP signaling, glutamatergic synapses, dopaminergic synapses, and ribosome-rich functions in the gut. The PWP group showed increases in recombinant tissue inhibitors of metalloproteinase 3, and nucleotide-binding oligomerization domain, leucine rich repeat, and pyrin domain containing proteins 6 in the substantia nigra and decreased parkin, gasdermin D, recombinant tissue inhibitors of metalloproteinase 3, and nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing proteins 6 in the duodenum. In conclusion, this study combined gut microbiota, metabolomics, and proteomics to evaluate the mechanism of action of Ping-wei-san plus on Parkinson's disease and revealed that PWP herbal treatment modulated gut microbiota, altered metabolite biological pathways, and affected functional pathway protein expression in Parkinson's disease mice, resulting in therapeutic effects.

Shashtika Shali Pinda Sweda in the Management of Cerebral Palsy: A Critical Review

Children are the strength of a nation. Childhood period is the foundation of the life and it is here that seeds for healthy life are laid down; disabled children are of great concern to family as well as the society. Cerebral Palsy (CP) is the leading cause of chronic disability in children, making them socially apart. According to WHO (World Health Organization) approximation, in India estimated incidence of Cerebral Palsy is around 3 / 1000 live births. CP is the manifestation of intrauterine pathologies, intrapartum complications, and the postnatal sequel, especially among preterm neonates. A double hit model theory is proposed suggesting that an intrauterine condition along with intrapartum or postnatal insult lead to the development of CP. Swedana (fomentation) is defined as the process by which the sweat or perspiration is produced in the body by using various methods. Inducing perspiration by using heated round Pinda (bolus) of special rice i.e. Shashtika Shali prepared in milk and processed with herbal decoction is referred as Shashtika Shali Pinda Sweda. This belongs to the category of Agni sweda as well as Snigdha sweda. A pinda (bolus) made up of Shashtika Shali (a type of rice harvested in 60 days) prepared in milk processed with herbal decoction is tied in bolus to rub against the whole body or afflicted part of the body so as to provide heat, nutrition and strength to the pain afflicted joints, muscles or body parts. Shashtika Shali pinda Swedana is found to be effective against various symptom of CP.

Gold Bhasma and Silver Parpam Used in Indian Traditional Medicines. Scientific Validation of their Interaction with Human Cells

Bhasmas in Ayurveda and parpams in Siddha medicine are unique herbo-metallic/mineral preparations, effective remedies, fabricated from highly purified metals, treated with a variety of herbal decoctions and incinerated at high temperatures to, finally, obtain a metal ash, having a significantly reduced size and free of toxic effects. The processing techniques of bhasmas and their use as medicines have been described in ancient texts of Ayurveda such as Rasa Shastra, Charaka Samitha and Sushruta Sambita. It has been emphasized that, while Siddha medicine is close to Ayurveda, Siddha has been closely linked to the Tantric religious movement, traced back to the 6th century AD and it is believed that Alchemy played a more central role in Siddha medicine than in Ayurveda. Some of the most important bhasmas and parpams are briefly described; their fabrication and properties are mentioned. The study of the interaction of gold bhasma and silver parpam with human cells investigated by our group is described in the second part of this work. In this section, the cellular uptake and localization of the gold and silver particles in cancerous and normal cells have been elucidated by using, principally, the hyperspectral imaging method that combines the image with the spectral information.

Influence of antimicrobial substances of plant origin on the sensitivity of bacterial biofilm to antimicrobial drugs

The widespread antibiotic resistance is associated with the formation of special forms of microbial communities — bacterial biofilms with increased resistance to the impact of various physicochemical factors, including antimicrobial drugs. In this regard, the investigation of antibacterial agents, including herbal preparations that could affect bacterial biofilms, seems to be of current interest. The aim of this work was to identify antibacterial substances of plant origin that can most effectively affect bacterial biofilms. The effect of antibacterial decoctions of lingonberry, sage, and chamomile plants on the sensitivity of bacteria to antimicrobial drugs was assessed. The susceptibility of bacteria to antimicrobial drugs, when combined with herbal decoctions, was determined by the disk-diffusion method. The morphophysiological properties of biofilms were illustrated by light and scanning electron microscopy. The ability of lingonberry, sage, and chamomile decoctions to increase the sensitivity of bacteria to antimicrobial drugs of predominantly bactericidal pharmacological action was revealed. Decoctions of medicinal plants prevented the formation of complete bacterial biofilms. The obtained results indicate the antibiofilm activity of lingonberry, chamomile and sage decoctions, which contribute to a decrease in the level of antibiotic resistance of bacteria and a decrease in the load during antimicrobial therap.

THE ROLE OF AYURVEDIC MEDICINE AND SHIRODHARA IN AVASADA W.S.R DEPRESSION – A REVIEW STUDY

Depression, a prevalent mental health issue globally, affects millions of individuals and manifests as persistent feelings of despair, loss of interest, and hopelessness. It impacts people irrespective of their age, gender, or background, often stemming from a complex interplay of genetic, environmental, and psychological factors. Symptoms vary among individuals but commonly involve enduring sadness, reduced energy, disrupted sleep, and disinterest in previously enjoyable activities. Severe cases may escalate to suicidal ideation and actions. Shirodhara, an ancient Ayurvedic therapy, entails the gentle pouring of herbal oils, medicated milk, or decoctions onto the forehead in a continuous rhythmic flow from a specific height. This unique treatment is administered for a defined duration to achieve therapeutic effects. Depression treatment encompasses a multifaceted approach involving medication, therapy, and lifestyle adjustments. Medicinal solutions like selective serotonin reuptake inhibitors (SSRIs) and tricyclic antidepressants (TCAs) are commonly prescribed to alleviate symptoms. Psychotherapy, such as cognitive-behavioral therapy (CBT), aids in acquiring coping strategies and fostering a more positive life perspective. Several risk factors contribute to depression, encompassing genetic predisposition, stressful life events, chronic ailments, and substance misuse. Seeking timely assistance upon experiencing depression symptoms is crucial, as early intervention enhances the effectiveness of treatment.

Abstract 2646: The effect of Chinese Traditional Medicine Herbal Decoction Tea on respiratory allergy profile region XVII and possibility of affecting the liver as seen in respiratory allergy (IgE) reaction as well as in the Aspartate Aminotransferase (AST) and Alanine

Abstract 2646: The effect of Chinese Traditional Medicine Herbal Decoction Tea on respiratory allergy profile region XVII and possibility of affecting the liver as seen in respiratory allergy (IgE) reaction as well as in the Aspartate Aminotransferase (AST) and Alanine

A Case Report of a Patient with Chronic Rupture of Gastrocnemius Muscle by Hwata 153 and Herbal Decoction

A Case Report of a Patient with Chronic Rupture of Gastrocnemius Muscle by Hwata 153 and Herbal Decoction

A Review of Clinical Studies for Chinese Medicine Treatment of Idiopathic Thrombocytopenic Purpura Using the CNKI Database

Objectives: The aim of this study was to analyze the latest clinical studies on Korean medicine treatment of idiopathic thrombocytopenic purpura (ITP) in the Chinese National Knowledge Infrastructure (CNKI) database.Methods: We searched the last 6 years of clinical studies discussing Oriental medicine-based treatments for ITP in the CNKI database. A meta-analysis of 13 RCTs was performed by synthesizing the outcomes, including the measured platelet count and total effective rate. The quality of the studies was assessed using Cochrane’s risk of bias (RoB) tool. RevMan 5.4.1 software was used for data analysis.Results: Of the 15 selected studies, 1 was a non-randomized controlled trial (nRCT), 2 were case series, and 12 were randomized controlled trials (RCTs). Treatments in all studies included oral herbal medicine. The most frequently used herbal decoction was the Liangxue Jiedu prescription (凉血解毒方), and the most commonly used herb was Agrimonia pilosa (仙鶴草), Astragali Radix (黃芪), Fossilia Glycyrrhizae Radix et Rhizoma (甘草), and Rehmannia glutinosa Liboschitz ex Steudel (地黃). The meta-analysis showed significantly better improvement in platelet counts and total effective rate for ITP in the treatment group than in the control group.Conclusion: Treatment with herbal medicine was effective in treating ITP. However, the significance of this conclusion is somewhat limited due to the low quality of the available studies. Multifaceted and scientifically designed clinical studies are required to develop treatments for ITP based on Korean medicine. The results of this study could be used as basic data for further ITP studies.

PEDAGOGICAL ASPECTS OF THE USE OF NATURAL MEANS OF HEALTH IMPROVEMENT BASED ON THE PRINCIPLES OF A SYNERGISTIC APPROACH

The article considers ways to increase the effectiveness of the use of natural remedies. It was found out that it is important to build and implement an individual health program taking into account biological, physiological, psychological, biorhythmological and other factors that affect human life. Revealing the holistic principle of perception of the world and each of its components, synergetics offers a different vision of the mechanisms of the formation of integrity, its transformation and development through the mutual influence and unity of various constituent elements. The state of health is determined by the harmony of the functioning of the organism as a whole, i.e., the coordinated interaction of systems, organs, energy-informational structure, and all cells of the body. The task is to restore balance and harmony using means of influencing both physiological systems and the psyche, which are equally important for maintaining internal harmony and balance between "conflicting", opposing forces: heat-cold, acid-alkali, movement-rest, assimilation-dissimilation, oxygen-carbon dioxide, tension-relaxation, etc. According to the principles of synergism, the main role is not played by medicinal products as such. There are many health factors, since everything that surrounds us affects a person in one way or another, changing the balance in the body, disrupting or restoring harmony. Anything can serve as a medicine: a glass of water or juice, regular walks, a clove of garlic or a decoction of herbs, a change in climate or management of thoughts and emotions, correct posture or the use of water procedures - all these simple components of everyday life can effectively balance various processes in the body and work as medicine. If we look at nature through the eyes of a doctor looking for medicines, we can say that we live in a world of medicines. It has been proven that the synergistic approach opens up new opportunities for preserving or restoring the balance and harmony of the life processes of the human body, and therefore, improving the effectiveness of the health-care process. Practical recommendations on the use of the most important health-improving means are given.

Comparison of the incidence of adverse reactions between herbal decoction and Chinese patent medicine combined with western medicine in treatment of COVID-19: A protocol for systematic review and meta-analysis.

Coronavirus disease 2019 (COVID-19) is an acute respiratory infectious disease that has led to millions of confirmed cases and deaths worldwide. Traditional Chinese Medicine plays an irreplaceable role in the treatment and prevention of epidemics in China. Western medicine improves clinical symptoms as well as bringing many adverse reactions. The combination of Chinese and western medicine can significantly improve the clinical symptoms and efficacy of patients. Currently, there is a lack of systematic reviews on the comparison of the incidence of adverse reactions between the difference treatments. We conducted this study to evaluate the comparison of the incidence of adverse reactions between herbal decoction and Chinese patent medicine combined with western medicine in treatment of COVID-19. Randomized controlled trials in 9 databases from December 2019 to September 2022, will be included, without language restrictions. Two independent researchers will screen and select studies, extract data, and evaluate the study quality. The Cochrane risk-of-bias tool for randomized controlled trials will be used to assess the risk of bias in the included studies. Statistical analyses will be conducted using Review Manager. Our findings will compare the incidence of adverse reactions between herbal decoction and Chinese patent medicine combined with western medicine in treatment of COVID-19, which will be disseminated in a relevant conference and published in a peer-reviewed publication. This study will not include personal information. Ethical approval is not required for this study. CRD42022371001.

Chaihu-Shugan-San ameliorates tumor growth in prostate cancer promoted by depression via modulating sphingolipid and glycerinphospholipid metabolism.

Background: Psychologic depression is a pivotal pathological characteristic and has been shown to promote prostate cancer (PCa) progression. Chaihu-Shugan-San (CSS), a well-known Chinese herbal decoction, exhibits efficacy in the treatment of stress-accelerated PCa. However, the underlying mechanism of CSS in resisting PCa growth is still unknown, and further study is needed. Objective: To evaluate the effects of CSS on stress-accelerated PCa in a BALB/C nude mice model and to investigate the underlying mechanisms. Methods: PC-3 cells were implanted into BALB/C nude mice, and the stressed mice were exposed to chronic unpredictable mild stress (CUMS) to study the effects of CSS. The PCa growth were evaluated by tumor volume and tumor weight. Analyses of depression-like behaviors were evaluated by sucrose consumption test, tail suspension test and open field test. Network pharmacology was used to analyze the potential targets and signaling pathways of CSS against PCa. Untargeted lipidomics were used to analyze the serum lipid profiles and further elucidate the possible mechanism. Results: In the CUMS stressed PCa mice, CSS can restrain tumor growth with reduced tumor volume and tumor weight, and depression-like behaviors with increased sucrose consumption, reduced immobility duration, and increased total distance and center distance. Network pharmacology suggested that the lipid metabolism-related pathways are the most likely potential targets of CSS against PCa. Using untargeted lipidomics analysis, 62 lipids were found to have significant changes in PCa mice under CUMS treatment. The levels of glycerophospholipids containing phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidylglycerol (PG), except PC (18:0_22:6) and PC (18:0_20:4), were significantly increased. Likewise, the levels of all sphingolipids (including sphingomyelin (SM), ceramides (Cer) and hexosyl-1-ceramide (Hex1Cer)) and diglyceride (DG) (32:1e) were significantly increased. CSS water extract was found to contribute to restore 32 lipids including 6 sphingolipids, 25 glycerophospholipids and 1 glyceride. Conclusion: This study is the first to delineate the lipid profile of stressed PCa BALB/C nude mice using untargeted lipidomics analysis. CSS restrained tumor growth and ameliorated depression-like behaviors by reprogramming lipid metabolism. Intervention of lipid metabolism could be a preventive and therapeutic approach for PCa patients with depression.

The trend of key results and strategies for improvement of Herbal Medicine Consumption Survey

Objectives: To identify changes in the subjects and methods of Herbal Medicine Consumption Survey, and analyze trend of the key resultsMethods: The population, methods, and items of the basic reports of all Surveys on Consumption of Herbal Medicine(HM) were organized in a time-series manner. The analysis items were trend in the purchase of standardized HM; consumption value share, and price of prepared HM; type of herbal dispensary; and awareness of HM policy in Koran Medicine(KM) institutions.Results: The price of HM preparations showed an upward trend in 2011, 2014, and 2017 surveys, and decreased in the 2020 survey. However, despite this recent decrease, the 2021 survey also saw the highest proportion of HM users reporting that price of herbal decoction is expensive. Furthermore, the demand for expanded coverage of herbal decoction was the greatest for the expansion of health insurance benefits. Efforts such as adjusting the number of covered diseases and the cost of health insurance coverage would be necessary. Regarding decoction dispensaries the proportion of HM hospitals using only extramural herbal dispensaries increased. Finally, the consumption of HM and the size of the HM industry has continued to expand due to the large-scale branding of KM institutions and the expansion of health insurance coverage.Conclusion: Future surveys must standardize and unify the items for the time-series continuity and compare the results with government statistics reports on HM to increase reliability. Moreover, specialized survey items may be developed for KM, to establish a better and efficient distribution system for domestic HMs.

Clinical effectiveness of the use of the veterinary drug “Amoksidev 15” in the conservative treatment of bitches for pyometra

Among diseases of the reproductive organs of bitches, pyometra is most often registered. Objective diagnosis of this disease in dogs by practicing veterinarians is difficult and uninformative. In addition, to date, effective methods of treatment for pyometra, which allow for restoring the productivity of the animal, have yet to be sufficiently developed. The purpose of the research was clinical trials of an antibacterial drug based on amoxicillin trihydrate, which is used for the medical treatment of pyometra in bitches – “Amoksidev 15”. The research was conducted on purebred and purebred bitches treated in the clinic with a pyometra (empyema) diagnosis of the uterus. Conservative treatment of bitches, using the antibacterial drug “Amoksidev 15” as part of a complex scheme, which included the following drugs: Dinolytic (enzaprost-F), No-shpa, Devivit Complex, Tioprotectin 2.5 %, herbal decoctions (nettles, raspberries, chamomile, boar's uterus) led to the recovery of 70 % of sick animals. When using drug therapy (animals of the control group), the clinically expressed manifestation of the disease lasted eight days on average. In the blood of these animals, an increase in the number of erythrocytes by 23.2 % was observed, and the number of leukocytes increased by 39.0 %. Therefore, the complex therapy used in the experiment resulted in the faster restoration of the reference values of the body's physiological indicators than in the control group animals, which included Enroxil 5 % as part of the complex therapeutic scheme. The prospect of further research will be clinical trials of the antibacterial drug “Amoksidev 15” in complex therapy for treating pyometra in cats.

Efficacy and safety of Sri Lankan traditional medicine regimen for knee osteoarthritis: study protocol for an open-label, active comparator, randomized controlled trial

BackgroundKnee osteoarthritis (KOA) is the most common form of arthritis, causing disability and impaired quality of life especially in the elderly. Sri Lankan traditional medicine (STM) is widely used to treat OA, but no clinical trial evaluated on STM regimens for KOA to discuss their safety and efficacy in the treatment. The aim of this study is to compare the efficacy and safety of STM regimen for KOA in comparison to recommended conventional pain management therapy over a period of 8 weeks on relieving the condition.Study designThis is a clinical trial following a protocol-driven open-labeled randomized controlled study enrolling patients with KOA that will be conducted as a single-center trial in the National Ayurveda Teaching Hospital, Sri Lanka. Rasnadvigunabhagasaya herbal decoction (RDBD) and an herbal pill Yoaraja Guggulu were selected as the rescue medication for treating joint disorders. The two Ayurvedic dosage forms will be tested against the non-steroidal anti-inflammatory drugs tab paracetamol and tab ibuprofen as the rescue medication for their safety and efficacy. As test products for external application, oil with an herbal fomentation—Kumburuetaperumkayam Pottani (KAP)—and paste—Sandivadam Lepaya (SVL)—were selected. External applications will be tested against the diclofenac sodium gel and hot water fomentation. KOA patients will be allocated randomly into two arms, and the medications will be given orally for 60 days and externally for 30 days. The primary endpoint is the change in the score on the WOMAC after 08 weeks. WOMAC and KOOS will be recorded and compared between the two arms prior to visiting 1, at the end of 15 days and end of 30 days, and end of the 45 days and end of the second month and 3 months of follow-up. KOOS and WOMAC subscales, a pain disability index, a visual analog scale for pain and sleep quality, and a quality-of-life index are used as secondary outcome measurements.DiscussionThis clinical trial will be able to provide evidence-based scientific data on Sri Lankan traditional medicine regimens in the management of KOA. This trial is expected to develop capacity to scientifically evaluate various STM that are claimed to have efficacy in treatment of various disease conditions.Trial registrationISRCTN58050062.

Natural Small-Molecule-Based Carrier-Free Self-Assembly Library Originated from Traditional Chinese Herbal Medicine.

The carrier-free self-assembly of small molecules opens a new window for the development of nanomaterials. This study is dedicated to developing binary small-molecular self-assemblies derived from phytochemicals in traditional Chinese herbal medicine. Among them, Rhei Radix et Rhizoma and Coptidis Rhizoma are a common pair used in clinics for thousands of years. Here, we found that there were numerous spherical supramolecular nanoparticles (NPs) originated from Rhei Radix et Rhizoma and Coptidis Rhizoma decoction. Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) was used to analyze the composition of the supramolecules, and a total of 119 phytochemicals were identified (23 anthraquinones, 31 alkaloids, 24 organic acids, 8 tannins, and other components). Isothermal titration calorimetry (ITC) showed that the interaction between Rhei Radix et Rhizoma and Coptidis Rhizoma was a spontaneous exothermic reaction, indicating that their phytochemicals had the property of self-assembly and interacted to form supramolecules in the decocting process. Furthermore, scanning electron microscopy (SEM), UV, IR, NMR, and ITC were used to verify that rhein and coptisine could self-assemble into nanofibers (Rhe-Cop NFs), while emodin and coptisine could self-assemble into nanoparticles (Emo-Cop NPs). The formation mechanism analysis of the self-assemblies revealed that they were induced by electrostatic attraction, hydrogen bonding, and π-π stacking, forming nanospheres of about 50 nm and nanofibers. The current study not only provides an idea of discovering carrier-free self-assemblies from traditional herbal medicine decoction but also supplies a reference for the design of binary self-assembly of small molecules in the future.

A network pharmacology approach and experimental validation to investigate the anticancer mechanism and potential active targets of ethanol extract of Wei-Tong-Xin against colorectal cancer through induction of apoptosis via PI3K/AKT signaling pathway

Ethnopharmacological relevanceWei-Tong-Xin (WTX), derives from the Chinese herbal decoction (CHD) of Wan-Ying-Yuan in ancient China, has been shown to be effective therapeutic herbal decoction for treating gastrointestinal diseases. Present studies have demonstrated that WTX had potential to alleviate the symptoms of gastrointestinal inflammation, gastric ulcer and improve gastric motility. Aim of the studyThe study primarily focused on exploring the therapeutic effect and possible pharmacological mechanism of WTX on colorectal cancer (CRC) based on network pharmacology, in vitro and in vivo experiments. Materials and methodsFirstly, colorectal cancer and WTX associated with targets were searched from GeneCards database and TCM Systems Pharmacology Database and Analysis Platform (TCMSP) respectively. The protein–protein interaction (PPI) network also was constructed to screening key targets. In addition, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were applied to predict the underlying biological function and mechanism involving in the anti-colorectal cancer effect of WTX. Next, CCK-8, colony formation and transwell assays were performed to verify the influence of proliferation and metastasizing ability of HCT116 cells after treated with WTX. Cell cycle, apoptosis and reactive oxygen species (ROS) were analysis by flow cytometry. Hoechst 33258 staining was conducted to observe nuclear morphology changes. Protein expression of apoptosis and PI3K/AKT signaling as well as mRNA expression of ferroptosis and apoptosis were determined by Western Blotting and RT-qPCR. The effects of WTX and LY294002 combination on the PI3K/Akt/mTOR signaling pathway were measured by Western Blotting. Finally, the xenograft tumor mouse model was established by subcutaneous injection of CT26 cells to measure tumors volume and weight. Hematoxylin and eosin (HE) staining and immunohistochemical analysis were used to observe the pathological changes and the protein expression in tumor tissues. ResultsThere were 286 potential treatment targets from 130 bioactive compounds in WTX, 1349 CRC-related targets were identified. Eleven core targets (TP53, AKT1, STAT3, JUN, TNF, HSP90AA1, IL-6, MAPK3, CASP3, EGFR, MYC) were found by PPI network analysis constructed of 142 common targets. The results of KEGG enrichment displayed PI3K/AKT signaling pathway as core pathway. After the treatment of WTX, the inhibitory of viability, metastases and cell cycle arrest at G2/M phase were observed in HCT116 cells. Moreover, WTX induced an increase in the expression of apoptosis proteins (Bak, cytochrome c, cleaved caspase-9/caspase-9 and cleaved caspase-3/caspase-3) and the levels of ROS and MDA, a decrease in the expression of PI3K/AKT signaling related proteins (PI3K, p-PI3K, p-AKT/AKT and p-mTOR/mTOR) and the level of SOD. WTX treatment significantly reduced the tumor weight, increased cleaved caspase-3 positive area and decreased that of ki67 in xenograft mouse model. ConclusionThrough a network pharmacology approach and in vitro experiments, we predicted and verified the effect of WTX on colorectal cancer cells mainly depended on the regulation of intrinsic apoptosis via PI3K/AKT signaling pathway, and further animal experiments proved that WTX has a good anti-colon cancer effect in vivo.

Efficacy and safety of Chinese herbal medicine Danggui Sini decoction for knee osteoarthritis: A protocol for systematic review and meta-analysis.

Knee osteoarthritis (KOA) often causes joint pain, weakness and mobility disorders, which seriously affects people's daily life and makes them unable to work and study normally. Traditional Chinese medicine (TCM) prescription Danggui Sini Decoction (DGSND) has been widely used in clinical practice and achieved good results. But there is no high-level evidence to support this result. The aim of this study is to evaluate DGSND's efficacy and safety in the management of KOA. We will search 7 electronic databases including Chinese National Knowledge Infrastructure (CNKI), Wanfang Data (WF), Chinese Scientific Journals Database (VIP), Chinese databases SinoMed (CBM), PubMed, Embase, and Cochrane Library databases. All the publications, with no time restrictions, will be searched without any restriction of language and status, the time from the establishment of the database to September 2022. Two reviewers will independently assess the quality of the selected studies, NoteExpress and Excel software will be used to extract data, and the content will be stored in an electronic chart. Different researchers will separately screen the titles and abstracts of records acquired potential eligibility which comes from the electronic databases. Full-text screening and data extraction will be conducted afterward independently. Statistical analysis will be conducted using RevMan 5.4 software. This study will compare the effects of DGSND and any other different methods on patients with KOA to provide high-quality, evidence-based clinical recommendations. The study provides a trustable clinical foundation for DGSND in the treatment of KOA.

Clinical Study on Acupoint Application of Jianpi Powder Combined with Chinese Medicine Decoction in Chronic Atrophic Gastritis

Background To investigate the clinical effects of acupoint application with Jianpi Powder combined with Chinese herbal decoction in chronic atrophic gastritis (CAG).Methods: 140 patients with CAG of spleen deficiency and phlegm stasis type from January 2018 to January 2020 were selected and divided into control and observation groups, with 70 cases in each group. The patients in control group was given Chinese medicine decoction, and those in the observation group was given acupoint application of Jianpi Powder combined with Chinese medicine decoction. The clinical efficacy, the levels of gastrograph index and inflammatory factors, life quality and the occurrence of adverse effects were compared.Results: After treatment, the total effective rate of the observation group was obviously higher than that of the control group. The amplitudes of the pre-prandial and postprandial electrogastrograms were obviously higher, and those in the observation group were obviously higher compared with the control group. Interleukin-8 (IL-8), interleukin-11 (IL-11), and tumor necrosis factor (TNF-α) were decreased than those before treatment, and those in the observation group was lower. The scores of physical function, health status, social function, and mental health were obviously higher than those before treatment, with the significantly increase in patients of the observation group . There was no significant difference in the incidence of adverse effects.Conclusion: Acupoint application of Jianpi Powder with traditional Chinese medicine decoction has a significant effect on patients with CAG, with clinical symptoms improvement, inflammation reduction, and the recovery of gastric motility promotion.

Efficacy and safety of Chinese herbal medicine Buzhong Yiqi decoction for postmenopausal women with osteoporosis: A protocol for systematic review and meta-analysis.

Postmenopausal osteoporosis (PMOP) is a disorder of bone metabolism caused by estrogen deficiency in women after menopause, which manifests clinically as pain, spinal deformities, and even fragility fractures, affecting the quality of life of patients and possibly shortening their life span. Traditional Chinese medicine prescription Buzhong Yiqi Decoction (BZYQD) has been widely used in clinical practice and achieved good results. But there is no high-level evidence to support this result. The aim of this study is to evaluate BZYQD's efficacy and safety in the management of PMOP. We will search articles in 7 electronic databases including Chinese National Knowledge Infrastructure, Wanfang Data, Chinese Scientific Journals Database, Chinese databases SinoMed, PubMed, Embase, and Cochrane Library databases. All the publications, with no time restrictions, will be searched without any restriction on language and status, the time from the establishment of the database to September 2022. Two reviewers will independently assess the quality of the selected studies, NoteExpress and Excel software will be used to extract data, and the content will be stored in an electronic chart. Different researchers will separately screen the titles and abstracts of records acquired potential eligibility which comes from the electronic databases. Full-text screening and data extraction will be conducted afterward independently. Statistical analysis will be conducted using RevMan 5.4 software. This study will evaluate the efficacy and safety of BZYQD in the treatment of PMOP, to provide high-quality, evidence-based clinical recommendations. The study provides a trustable clinical foundation for BZYQD in the treatment of PMOP.

Zishen Pill alleviates diabetes in Db/db mice via activation of PI3K/AKT pathway in the liver

BackgroundThe rising global incidence of type 2 diabetes mellitus (T2DM) highlights a need for new therapies. The Zishen Pill (ZSP) is a traditional Chinese herbal decoction that has previously shown hypoglycemic effects in C57BL/KsJ-db/db mice, although the therapeutic mechanism remains unknown. This study aims to explore the underlying mechanisms of ZSP’s hypoglycemic effects using db/db mice.MethodsDb/db mice were divided into two groups: model group and ZSP group, while wt/wt mice were used as a normal control. ZSP was given to mice by gavage for 40 days. During treatment, blood glucose level and body weight were monitored continuously. Oral glucose tolerance test (OGTT) was performed at day 35. Blood and tissue samples were collected at the end of treatment for further analyses. Mice liver samples were analyzed with mRNA transcriptomics using functional annotation and pathway enrichment to identify potential mechanisms that were then explored with qPCR and Western Blot techniques.ResultsZSP treatment significantly reduced weight gain and glycemic severity in db/db mice. ZSP also partially restored the glucose homeostasis in db/db mice and increased the hepatic glycogen content. Transcriptomic analyses showed ZSP increased expression of genes involved in glycolysis including Hk2, Hk3, Gck and Pfkb1, and decreased expression of G6pase. Additionally, the gene and protein expression of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway, and Csf1 and Flt3 mRNA expression were significantly upregulated in ZSP group.ConclusionZSP treatment reduced the severity of diabetic symptoms in db/db mice. ZSP increased expression of genes associated with glycogen synthesis and glycolysis, and decreased gluconeogenesis via the enhancement of the PI3K/AKT signaling in the liver.