Herba-alba Extract Research Articles

The Therapeutic and Toxic Effects of Artemisia herba alba Extract on the Reproductive System and Fertility of Male Rabbits Oryctolagus cuniculus

The Therapeutic and Toxic Effects of Artemisia herba alba Extract on the Reproductive System and Fertility of Male Rabbits Oryctolagus cuniculus

In vitro anthelmintic activity of hydroethanolic extract of Artemisia herba aba and Juniperus phoenicea on abomasal Teladorsagia circumcincta of sheep.

The present study aimed to evaluate the in vitro anthelmintic activity of Juniperus phoenicea and Artemisia herba-alba against abomasal nematodes collected from naturally infected sheep. The anthelmintic activities of the extracts were assessed using egg hatch inhibition (EHIA) and adult motility assays (AMA) against Teladorsagia circumcincta. The parasites were stored in phosphate-buffered saline (PBS) until the in vitro assessment began. The leaf powders of J. phoenicea and A. herba-alba were extracted using the maceration procedure. Briefly, the leaf powders were mixed with a sufficient quantity of 99% ethanol and distilled water (80%/20%). The extracts were dissolved in a 3% dimethyl sulfoxide (DMSO) solution to improve water solubility. The hydroethanolic extracts were tested at concentrations of 1.562, 3.125, 6.25, 12.5, and 25 mg/ml. Adult motile worms were individually subjected to each treatment at laboratory temperature. The PBS and albendazole (25 mg/ml) were prepared and used as negative and positive controls, respectively. The test was repeated five times for each concentration. The results showed that both plant extracts exhibited anthelmintic effects. A. herba-alba extract at 25 mg/ml inhibited the motility of all T. circumcincta adult worms within 1 hour of administration, while J. phoenicea extract caused 100% mortality of adult worms after 4 hours at the same concentration. Neither of the extracts, at 1.562 mg/ml, had any inhibitory effect on motility. At 25 mg/ml, the A. herba-alba (98.67%) and J. phoenicea (96.24%) showed high activity with an inhibitory effect on hatchability compared to the other tested concentrations. The findings of the present study showed that hydroethanolic extracts of A. herba-alba and J. phoenicea leaves had in vitro anthelmintic activity on eggs and adult T. circumcincta of sheep.

Estimation of the Efficacy of Oral Administration of Egyptian Artemisia herba-alba Extract in Combating Thyroid Dysfunction in Diabetic Male Rats

Estimation of the Efficacy of Oral Administration of Egyptian Artemisia herba-alba Extract in Combating Thyroid Dysfunction in Diabetic Male Rats

Investigation of Antifungal Action of Fractions C17H31NO15 Isolated from Artemisia herba-alba extract versus Isolated Aspergillus niger from Zee maize.

Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning. Investigation of antifungal impact of various fractions of methanol extract of Artemisia herba-alba to Aspergillus niger as a plant pathogen. Artemisia herba-alba extract was purified using column chromatography, giving various antifungal fractions tested versus A. niger. The 6th fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02 ± 4.9 μg/ml, which was identified using Mass spectroscopy, 1HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated A. niger was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity. These results revealed the possibility of using Artemisia herba-alba methanol extract as a promising antifungal versus phytopathogenic fungi, especially A. niger after more verification of results.

Development and Evaluation of Bioconverted Milk with Anti-Microbial Effect against Periodontal Pathogens and α-Glucosidase Inhibitory Activity.

To decrease periodontal pathogens and increase the number of beneficial bacteria, probiotics and bioactive compounds made via microbial bioconversion are recently used. In addition, the interest regarding probiotics-mediated bioconversion with popular medicinal plants is increasing. Artemisia herba-alba, a type of wormwood, has recently been attention as a medicinal plant due to its various bioactive compounds. Therefore, we developed bioconverted milk containing A. herba-alba that effectively inhibited periodontal pathogens and α-glucosidase. To select the appropriate lactic acid bacteria for the probiotic candidate strain, 74 strains of lactic acid bacteria were screened. Among them, Lactiplantibacillus plantarum SMFM2016-RK was chosen as the probiotic due to its beneficial characteristics such as high acid and bile tolerance, antioxidant activity, and α-glucosidase inhibition. Based on the minimal bactericidal concentration against three periodontal pathogens, the following appropriate concentrations of Artemisia herba-alba extract were added to milk: 5 mg/mL of A. herba-alba ethanol extract and 25 mg/mL of A. herba-alba hot-water extract. Four bioconverted milks (BM), BM1, BM2, BM3, and BM4, were produced by combining L. plantarum SMFM2016-RK alone, L. plantarum SMFM2016-RK and ethanol extract, L. plantarum SMFM2016-RK and hot-water extract, and L. plantarum SMFM2016-RK with both extracts. As a result of antimicrobial activity, BM3 inhibited the growth of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis the most, and BM4 suppressed the growth of Fusobacterium nucleatum the most. In addition, bioconverted milk containing A. herba-alba (BM2, BM3, and BM4) inhibited α-glucosidase more effectively than BM1. The whole genome of L. plantarum SMFM2016-RK was obtained, and 3135 CDS, 67 tRNA, and 16 RNA were predicted. The genome annotation of L. plantarum SMFM2016-RK revealed 11 CDS related to proteolysis and amino acid metabolism and 2 CDS of phenolic acid-metabolizing enzymes. In conclusion, A. herba-alba-added milk bioconverted by L. plantarum SMFM2016-RK displayed both the growth inhibitory effect on periodontal pathogens and the α-glucosidase inhibitory activity; thus, it necessitates to evaluate the effects on the alleviation of periodontal diseases and glycemic control through future animal experiments.

Effectiveness of Zinc Oxide Nanoparticles and Artemisia herba alba Extract on the Chemical Composition of Soft Cheese

This study was conducted to assess the impact of ZnONPs and Artemisia herba alba extract loaded on manufacture edible gelatin in the chemical composition of cheese during the various storage periods and five transactions of cheese were manufactured which included the (T1) control sample, (T2) membrane of gelatin without adding, membrane of gelatin with loaded of ZnONPs (T3), membrane of gelatin with loaded Artemisia herba alba extract (T4) and (T5) was added ZnONPs and Artemisia herba alba extract on edible gelatin. The results of the cheese storage showed that the humidity rate had decreased morally ( p < 0.05) up to the end of the storage period and the transaction (T1) was more moisture loss to record (53.34), also found that protein, fat and ash ratios did not show moral differences at the time of one day compared with the control sample (T1) These ratios then gradually increased as long as conservation lasted and reached its highest values at 21 days. pH values have decreased morally to be at the end of the storage period between (6.10-6.26) compared with the control sample which was (5.78) and found that the fatty acid values of all transactions were morally low compared to the control sample (T1) At the time of one day, the ratio of free fatty acids rose and reached its highest values among (0.57-0.40)% for all treatments compared to the (T1) control treatment at the end of the storage period which reached (0.65%).

Effectiveness of Soft Cheese Fortified by Zinc Oxide Nanoparticles and Artemisia Herba Alba Extract on Blood Lipid Profile of Hypercholesterolemic Rats

The study aims to investigate the effect of artemisia herba alba extract and ZnONPs on the level of lipid profile that included total cholesterol, triglycerides, low-density lipoproteins, and at the level of liver enzymes that included (AST, ALT, ALP) in adult rats Sprague-Dawley and lipoproteinosis. Rats were distributed to six aggregates in six repeaters each. (T1) Control Group, (T2) Nutritious Group on high cholesterol leeches, (T3) Nutritions high cholesterol food aggregates and soft cheese dosage (2 g/kg) (T4), ZnONPs 10mg/ml (T5) Artemisia herba alba (150/ml), (T6) Artemisia herba alba with ZnONPs. After the end of the feeding period for the vital experiment of 28 days. The Results showed a moral decrease (p≤0.05) in the rate of cholesterol, triglycerides and low-density lipoproteins in the T3, T4 group, T5, T6 compared with T1, while T2 treatment continued to rise in blood fat because it was left untreated. A moral decrease in the level of liver enzymes (AST, ALT, ALP) was also observed for animals treated with T3, T4, T5, T6 compared to T2, which was left untreated, with an increase in the concentration of liver enzymes.

The Intensity of the Biological Effect and Stress of Extracts of the Aerial Parts of Artemisia herba-alba-on Algae (Klisinema persicum)

Inhibitory impact of Artemisia herba-alba extract on Klisinema persicum growth was investigated. This study looked at the effects of different concentrations of A. herba-alba extracts on the growth and antioxidant enzyme (superoxide dismutase and Catalase) activity of K. persicum in order to determine the optimal dose range for the good anti-algal action. Concentrations were used (2.5,5, 10, 20, 40,80 and 160) mg. L-1 as well as control. Results showed that both crude extracted, alcoholic extract, and aqueous extract of A. herba-alba had an obvious inhibitory effect on K. persicum growth, as such the obtained results demonstrated A. herba-alba crude extracted inhibited algal growth more efficiently than alcoholic extract and aqueous extract of A. herba-alba. This investigation found that a dose of 10 mg. L-1 with above 50% of IR of extracted crude, alcoholic extractand aqueous of A. herba-alba was best, based on changes in algal cell density and inhibitory ratio (IR). Additionally, after 4 days, the extracted A. herba-alba group saw a significant decline in Chlorophyll-a concentration and antioxidant enzymes activity, falling below the detection threshold. Our findings may pave the way for further investigation into the mechanisms underlying inhibitory effects on dangerous algae, which in turn could lead to the creation of novel anti-algal materials.

Ethanolic Extract of Artemisia herba-alba Ameliorates Hyperinsulinemia and Hyperglycemia-Induced HepG2 Cells Through Cell Survival Promotion, Oxidative Stress Mitigation, and Insulin Signaling Restoration

Background: In recent years, diabetes and its risk factors were linked to an augmented occurrence of cardiovascular diseases (CVD), which are considered major causes of morbidity and mortality in diabetes mellitus subjects. Hyperinsulinemia (HI) and hyperglycemia (HG) are recognized as insulin resistance-inducers, which can trigger several alterations in cellular biogenesis besides inflammatory signaling activation. As a folk medicine in many countries, Artemisia herbaalba has been preclinically studied for treatment of type 2 diabetes mellitus (T2DM) and showed beneficial effects on hyperglycemia. Methods: In the current work, we explored the potential mechanisms underlying the antihyperglycemic behavior of the ethanolic extract of A. herba-alba and its bioactive compounds. Results: Obtained data demonstrated that ethanolic extract of A. herba-alba enhances HepG2 viability and proliferation, decreases considerably the apoptosis, through the regulation of pro- and anti-apoptotic pathways implying p53, p21 and Bcl-2 genes expression and Pan caspases activation under IR concentration at the lowest concentrations. A. herba-alba extract treatment additionally exhibited a potential antioxidant effect, evidenced by the significant decrease in total intracellular reactive oxygen species (ROS) levels and the improved mitochondrial transmembrane potential in HG/HI-challenged HepG2 cells (p &lt; 0.001). Furthermore, A. herba-alba positively upregulated Irs1 and Irs2 as well as Akt/Pi3K pathway compared to insulin-resistant untreated cells, and subsequently stimulated glucose uptake in response to insulin infusion, as a consequence of its signal transduction restoration. Conclusion: Overall, the obtained data highlighted the beneficial effects of A. herba-alba ethanolic extract in ameliorating insulin signaling and liver cells metabolic balance, and shed the light for its use as a promising and safe therapeutic lead for the management of type 2 diabetes and underlying metabolic failures.

Extraction Yield, Phytochemicals Analysis and Certain In-Vitro Biological Activities of Artemisia herba alba Extracts

The objectives of this study were to determine the extraction yield and phytochemical composition of different Artemisia herba-alba (AHA) extracts, as well as to investigate in-vitro their antioxidant, anti-inflammatory, anti-glycation, and antibacterial activities. Four solvents with different polarities were used to prepare AHA extracts. The extraction yield was determined using the gravimetric method. The qualitative analysis was performed using the standard methods. The quantitative analysis, the antioxidant, and the anti-inflammatory activities were estimated using the colorimetric method. The anti-glycation activity was measured using the spectrofluorimetric method. The antibacterial activity was performed using an agar diffusion method. Our findings showed that 80% aqueous ethanolic extract significantly (p ≤ 0.05) compared to other extracts exhibited high extraction yield (15.3%), high phytochemical content (263.93±2.46 mg GAE/g E for total phenols), (40.94±1.45 mg QE /g E for total flavonoids), and (35.99±1.20 mg GAE/g E for total tannins), better Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging capacity (IC50 = 4.13±0.02 mg/mL) with no significant differences (p ≤ 0.05) when compared to methanolic extract, as well as high anti-glycation activity (IC50 = 3.96±0.09 mg/mL). The methanolic extract had a significant inhibitory effect against the majority of the tested bacteria ccompared to other extracts. The anti-inflammatory potential of distilled water extract was significantly (p ≤0.05) higher (IC50 = 2.96±0.06 mg/mL) compared to the rest yet failed to show any antimicrobial activity against all assayed bacteria. In summary, the solvent type had a significant effect on the chemical and biological characteristics of AHA. The diversity and significance of Artemisia herba-alba’s biological activities demonstrate its potential application within the pharmaceutical and food areas.

Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products

The rationale for undertaking this study is lethality of diabetes mellitus as predicted by 6.7 million deaths in 2021 and immense pharmacological potential of Artemisa herba-alba. The current research examined how Artemisia herba-alba extract (AHE) affects the peripheral artery disease in diabetic rats through lowering of advanced glycation end products (AGEs). The in-vitro AGE inhibiting potential of AHE was determined by spectrofluorimetric method. The blood glucose levels and HbA1c (A1C) of the rats from each group were determined by automatic analyser. The levels of AGEs in vascular smooth muscle cells (VSMCs) of different rat groups were observed through western blotting. Expression of COX-1 and COX-2 were determined by qRT-PCR. The AHE inhibition of AGEs formation was reported in vitro and exhibited an IC50 of 45 μg/mL which was significantly lower than that of standard AGEs inhibitor aminoguanidine (IC50: 60 μg/mL). Analysis of metabolic profiles revealed that AHE normalised the blood glucose, cholesterol, and triglycerides with no apparent changes on Hb1Ac levels. Western blot analysis showed that AHE exhibited protective effects in VSMCs of diabetic rats by inhibiting fabrication of AGEs. Moreover, the manifestation of COX-2 was inhibited by AHE in diabetic rats. However, the expression of COX-1 remained unaltered. Collectively, the results revealed AHE inhibits AGEs formation in vitro and in VSMCs of diabetic rats. These findings point towards the prospective of AHE applications towards the management of diabetic peripheral artery disease.

Protective effect of Artemisia herba-alba extract on the liver of diabetic albino male rats

Protective effect of Artemisia herba-alba extract on the liver of diabetic albino male rats

Antioxidant Activity and Polyphenols Content of Artemisia Herba-Alba Extract and their Cytotoxicity against Human Lung Cancer Cells NCI-N417

ABSTRACT Phytochemicals in the ethanol extract of Artemisia herba-alba were examined and evaluated for the antioxidant and cytotoxicity potential. The extraction was performed using an accelerated solvent extraction (ASE300), analyzed by GC-MS and the total phenolics was estimated by Folin–Ciocalteau method. The antioxidant activity of the extract was evaluated using three methods and its cytotoxicity was evaluated in-vitro on NCI-N417 tumor cell line using MTT test and flow cytometry. Twenty-one compounds were identified in the A. herba-alba extract, which was rich in phenolic compounds (248.56 ± 11.05 μg GAE mg−1 extract) and had antioxidant activity characterized by a high DPPH-radical-scavenging activity (IC50 = 69.01 ± 0.03 μg mL−1), inhibitory effect of β-carotene oxidation (95.75%±0.013), and reducing power (DO700nm = 0.751 ± 0.001). Ethanol extract from A. herba-alba demonstrated cytotoxic and antiproliferative activity.

Evaluation of the Effects of Artemisia herba-alba on Intestinal Parasite Infections in Domestic Pigeons (Columba livia) in Albeida City, Libya

The first aim of the current study is to investigate the presence of intestinal parasitic infections in domestic pigeons (Columba livia) in Albeida City, Libya.&#x0D; individually faces samples from 100 birds were collected and examined using direct smearing and the flotation method. The majority of the pigeons (60%) were likely to have mixed infections with one or two of intestinal parasites (Eimeria 40%, Capillaria 50%, Heterakis 8% and Raillietina 2%). The second goal is to determine the anti-parasites effects of Artemisia herba-alba aqueous extract on intestinal parasite infection. The birds were divided into 5 groups of ten each for different concentration of Artemisia herba-alba extracts (5 %, 10 %, 15 % 20 % and control group) administered orally once a day for 3 days. The results showed that there was no significant reduction in the numbers of internal parasites (Eimeria oocysts and helminths eggs) in infected pigeons.

Green synthesis of Ag nanoparticles in oil-in-water nano-emulsion and evaluation of their antibacterial and cytotoxic properties as well as molecular docking

Increasing use of silver nanoparticles (AgNPs) in various fields as mentioned recently like cosmetology, clinical nanotechnologies and medicine causes the growth of a nontoxic and green synthesis methodology. The present study provided a facile stable one-step synthesis of AgNPs in the presence of Artemisia herba-alba, Mentha, Rosmarinus and Ranunculus aqueous oil extract as reductant and stabilizer via oil-in-water nano-emulsion. Due to experimental results, in the presence of Artemisia herba-alba extract, AgNPs indicated smaller size, uniform shape and well dispersed NPs. Synthesized nanostructures characterized by using various techniques like XRD, UV–Vis, FT-IR, TEM, SEM, nano sizer and zeta potential measurements. The effect of some reaction parameters (the molar ratio of AgNO 3 , extract volume, pH and temperature) on the AgNPs investigated as well. Additionally, strong antibacterial effects of AgNPs found against four gram-negative and three gram-positive bacteria due to cell death according to increasing membrane permeability and bacterial wall integrity disruption. WST-1 exposed cytotoxicity potent index on the cancerous cell lines as established by the low IC50 value of 1 ppm. Binding energy of Ag metal with bacteria calculated by molecular docking and indicated that all bacteria have good interaction with silver NPs and confirmed antibacterial manner of Ag. Therefore, Produced AgNPs can be introduced as an effective antibacterial agent as well as a potent index of cytotoxicity effect on cancerous cell lines that leads to effective usage in nono-drug design and formulations in near future.

Anti-inflammatory and prolonged protective effects of Artemisia herba-alba extracts via glutathione metabolism reinforcement

Artemisia herba-alba (AHA) is a medicinal plant known for its preventive capacities against chronic diseases. Inflammation is a naturally occurring cellular response to noxious stimuli, and both acute and chronic inflammation are involved in human disorders. Preventing and limiting the negative impact of inflammation by using phytocompounds has attracted a lot of attention. Here, we show that AHA extracts are potent anti-inflammatory products at low doses in white blood cells, treated with phorbol myristate acetate (PMA), a pro-inflammatory trigger. Regardless of the mode of extraction, i.e. maceration or microwave, all extracts reduced on dose-dependent manner reactive oxygen species (ROS) production induced by the pro-inflammatory compound PMA in white blood cells and leukemic Jurkat cell line. The induction of the anti-inflammatory effect was evaluated by the measurement of glutathione metabolism (glutathione reductase, transferase, and peroxidase), and protein-free thiols in Jurkat cells. Jurkat cells incubated with low to high concentrated AHA extracts, ranged from 10 to 100 g/mL, induced a fast cell response measured at one and six hours. Interestingly, extracts from the microwave showed a stronger induction, 5 to 10 fold changes at early time points, and maintained over 72 h. On the other hand, AHA extracts showed a moderate anti-proliferative effect in the leukemic Jurkat line. We concluded that AHA extracts enhance directly anti-inflammatory cell response, earlier via ROS induction. Later, however, vigorously maintained, independently from ROS, suggesting the intervention of the cellular metabolism in enhancing these effects. Considering the preventive and anti-inflammatory potential of AHA, regular consumption is advisable, and phytotherapeutic applications are encouraged to treat local inflammations.

Improvement of the Ethanol activity by using leaves extract of Artemisia herb alba against Pseudomonas aeuroginosa

The current study objective to improve the influence of ethanol as antibacterial via mixing it with Artemisia herba-alba leaf extract by using a good diffusion method. Ethanol is well known as antimicrobial so that has been used as a hand sanitizer and disinfectant for a long time. Nowadays microbial resistance to disinfectants become a major problem in health care centers, Pseudomonas aeruginosa one of these microbes that tend to create biofilm in low concentrations of ethanol and considered a healthcare-associated pathogen causing nosocomial infections. Seven concentrations of ethanol 70, 60, 50, 40, 30, 20 and 10% were used and mixed with Artemisia herba-alba leaf extract at 50, 25, 12.5, 6.25, 3.12, 1.56 and 0.78 mg/ml in all concentration of ethanol. The results showed that the clear zone (C.Z.) of ethanol alone was 10, 10, 8 and 7 mm by using 70, 60, 50 and 40% respectively, well the low concentrations don't show any antibacterial effect. Additionally, the antibacterial of A. Herba-alba extracts also calculated, showing high C.Z. was 33, 29 and 27 mm at 50, 25 and 12.5 mg/ml, respectively. All the doses of mixture ethanol and A. Herba-alba extract showed a higher effect comparing to previous results, where 50 mg extract mixed with 70% and 60% ethanol showed C.Z. 39 mm also the low concentration of ethanol showed antibacterial effect up to 33 mm, Even 0.78 mg of extract show inducing in the effect of ethanol against P. Aeruginosa. In conclusion, mixing A. Herba-alba leaf extract can improve the antibacterial effect of ethanol.

Toxicological study of the effect in vivo and in vitro of Artemisia herba-alba aqueous extract in rats

Background: Artemisia herba-alba (AHA) is largely used in folk medicine in different countries. However, rare studies provided toxicological evaluation regarding their safety on human health. Objective: This study investigated the safety of the standardized aqueous extract of AHA, like used by patients, to evaluate their toxicity in vivo and in vitro. Materials and Methods: For toxicological evaluation in vivo we used acute (during 14 days) and sub-acute oral gavages in Wistar rats (rats treated daily for 42 days at 1–5 g/kg bw) and the 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay was performed to determine the level of cell viability and the degree of cytotoxicity in vitro (0–30 μg/ml) on cultured spleen cells. Results: The LD50was up to 2 g/kg. Signs of mortality and toxicity were observed after single doses and no-observed-adverse-effect levels in the sub acute toxicity was up to 2 g/kg bw. Compared to the control, the treatment did not produce any statistically significant changes on alanine aminotransferase and aspartate aminotransferase serum titer. However, for creatinine and urea serum value, a significant increase (P

Effect of Zinc Oxid Nanoparticles on the Oxidative Stress (Malonaldehyde MDA, Lipid Peroxidation Level LPO) and Antioxidants (GSH glutation)

The plant was selected as the source of the nanoscale study because it possesses more chemical compounds than the other plants,ZnO nanoparticles ( ZnONPs) were prepared from the plant Artemisia herba alba extract and from the flavonoid compound. The samples were diagnosed by Atomic Force Microsco (AFM) and Fourier transform infrared (FT-IR) spectroscopy , and effect ZnONPs the synthesis from Artemisia herba alba extract , from the flavonoid compound , Artemisia herba alba and flavonoid on the criteria for oxidative stress (Malonaldehyde MDA, Lipid peroxidation level LPO) and antioxidants (GSH glutation) were determine effective time and effective dose. The results showed that MDA and LPO showed a significant decrease compared with the control group, while the period of 32 days showed a significant decrease compared to 62 days. The analysis of GSH found that the coefficients of the study showed a significant increase compared with the control, while the period of 32 days showed a significant decrease compared with 62 days.

Evaluation of anthelminthic activity of both pomegranate peels and Artemisia herba-Alba extracts in comparison with praziquantel in experimentally infected mice with Hymenolepis nana

Background Praziquantel (PZQ), is the drug of choice in the treatment of Hymenolepis nana infection, although it has some adverse effects (side effects and toxicity). Natural biological products that have minimal side effects and more safe. Many researchers demonstrated the antimicrobial and antiparasitic therapeutic effect of pomegranate and Artemisia. Objective Our study aims to evaluate the therapeutic effect of pomegranate and Artemisia as an alternative treatment in H. nana infection. Subjects and Methods 32 male albino mice were inoculated with human fecal sediments containing H. nana eggs (about 100 eggs/gm) and were divided into four groups after proven to be infected with H. nana: Group 1; treated with Pomegranate peels extract, Group 2; treated with Artemisia herb-Alba extract, Group 3; treated with PZQ, and finally, Group 4; treated with Pomegranate peels and Artemisia herb-Alba mixture’ extract. Results Both Pomegranate peels and Artemisia herb-Alba extracts presented a significant decrease (P-value = 0.002 and 0.001, respectively) in the number of viable eggs after 7 days of treatment in comparison to the first day. Pomegranate peels and Artemisia herb-Alba extracts ‘results established anthelmintic effect against H. nana. Conclusion Our results showed that both Pomegranate peels and/or Artemisia herb-Alba extracts can be used as alternative, less expensive and more safe therapeutic approach in H. nana infection.