HER2 In Gastric Cancer Research Articles

Expression and clinical significance of EGFR and Her-2 in gastric cancer

Objective To analyze the relationship between the survival and expressions of EGFR and Her-2, and explore the prognostic significance of EGFR and Her-2 in gastric cancer patient by detecting the expressions of EGFR and Her-2 in gastric cancer specimens. Methods EGFR and Her-2 were detected by immunohistochemistry. The relation between their expressions with their clinical characteristics and prognosis were analyzed. Results The over-expression rates of EGFR and Her-2 are 27.4 % and 32.1% respectively in 84 cases. The over-expression rates of EGFR and Her-2 in patients with Ⅲ + Ⅳ stages were significantly higher than those with Ⅰ + Ⅱ stages(respectively, P =0.014 and P =0.005). The positive rate in patients with T3+T4 stages were significantly higher than those with T1+T2 stages (P=0.033); and the positive rate in patients with positive lymph node was higher significantly than that with negative (P =0.033). The OS and PFS in over-expression patients were significantly longer than low-expression. Conclusion The overexpression of EGFR and Her-2 could be used to evaluate the degree of differentiation and prognosis of patients with gastric cancer. Key words: Stomach neoplasms; EGFR; Her-2

Down-Regulation of Human Epidermal Growth Factor Receptor 2/neu Oncogene by Corosolic Acid Induces Cell Cycle Arrest and Apoptosis in NCI-N87 Human Gastric Cancer Cells

Overexpression/amplification of human epidermal growth factor receptor (HER)2/neu (erbB-2) oncogene plays a causal role in carcinogenesis and correlates with a poor clinical prognosis. However, little is known about HER2 in gastric cancer. In this study, we explored the pharmacological activities of natural triterpenoid corosolic acid (CRA) in HER2 signaling and its role in gastric cancer development and progression. In this study, CRA dramatically inhibited HER2 expression in a dose- and time-dependent manner, effectively inhibited cell proliferation, and induced G(0)/G(1) arrest through the induction of p27(kip1) and cyclin D(1) down-regulation. CRA exposure enhanced apoptotic cell death, as confirmed by caspase-3 and poly (ADP-ribose) polymerase cleavage activities. CRA inhibited signaling pathways downstream of HER2, including phospho-proteins such as Akt and Erk. In addition, CRA combined with adriamycin and 5-fluorouracil enhanced this growth inhibition, but not with docetaxel and paclitaxel. These findings demonstrate that CRA suppresses HER2 expression, which in turn promotes cell cycle arrest and apoptotic cell death of gastric cancer cells, providing a rationale for future clinical trials of CRA in the treatment of HER2-positive gastric cancers.

Expression of HER2 in gastric cancer: Comparison between protein expression and gene amplification using a new commercial kit

Expression of HER2 in gastric cancer: Comparison between protein expression and gene amplification using a new commercial kit

Expression of HER2 in gastric cancer: Comparison between protein expression and gene amplification using a new commercial kit

4053 Background: Trastuzumab, a monoclonal antibody to HER2 protein, is widely used in patients with HER2-overexpressing breast cancer. Trastuzumab has also shown antitumor activity in human gastric cancer (GC) xenograft models with HER2 overexpression, however, the concordance of protein expression and gene amplification of HER2 in GC cells and the feasibility of using biopsy specimens has not been clarified Aim: The purpose of this study was to evaluate the frequency of HER2 overexpression and the concordance between protein expression and gene amplification in surgical and endoscopic biopsy GC specimens using two commercial immunohistochemical(IHC) kits and fluorescence in situ hybridization (FISH). Materials and Methods: The specimens consisted of formalin-fixed, paraffin-embedded sections of biopsy specimens and surgically resected tumors in 200 cases with invasive GCs who underwent surgery at the National Cancer Center Hospital East. The IHC analysis was performed using the Hercep Test kit (DAKO Cor...