Rat Hepatic Tissue Research Articles

INFLUENCE OF REPEATED SUBTOTAL HEPATIC RESECTIONS ON IN VITRO SURVIVAL OF RAT LIVER CELLS.

SummaryHepatic tissue from immature rats demonstrates minimal cell yield in tissue culture while hepatic tissue from mature rats (> 230 g) does not survive under the conditions employed. Mature rat (> 230 g) hepatic tissue obtained following repeated subtotal hepatic resections in the same animal demonstrates relatively large cell yield in tissue culture, using the same technique. The yield in tissue culture using hepatic tissue from mature rats following repeated resection is greater than in any of the control groups, including the most immature (weanlings) rats, and markedly greater than in tissue obtained following single hepatic resection.

On the metabolic alteration of pteroylglutamic acid.

SummaryThe liver appears to be the main site of the conversion of pteroglyglutamic acid (PGA) to citrovorum factor (CF) in the body. Other tissues had very limited capacity to form CF, with the exception of bone marrow which, relative to the small amount present in this tissue, formed an appreciable quantity of the factor. The hepatic tissue of rats which had been depleted of folic acid and CF was flooded with CF within one hour following the intraperitoneal injection of PGA. Homogenates of rat or chick liver, when incubated with PGA under an atmosphere of nitrogen, were capable of forming CF. Little CF was found when similar preparations were incubated under oxygen. The yield of CF was consistently increased by the presence of ascorbate in the incubation mixture.

Acid and Alkaline Phosphatase of Serum and Hepatic Tissue of Rats Fed Carcinogens

Acid and Alkaline Phosphatase of Serum and Hepatic Tissue of Rats Fed Carcinogens

Note on the Splitting of α-α Diglycylaminopropionic Acid Hydrochloride in Extracts of Rat Hepatic Tissues

Note on the Splitting of α-α Diglycylaminopropionic Acid Hydrochloride in Extracts of Rat Hepatic Tissues