Objective To evaluate the effect of hepatic artery angiography on liver perfusion using two contrast agent with different concentrations and injection rates and analysed the nursing factors. Methods 104 patients underwent liver perfusion examination by 320 slices CT with Omnipaque 350 and Ultravist 370 contrast agent, and then they were respectively divided into two groups based on different injection rates (4, 6ml/s).Hepatic artery CT peak value and vascular reconstruction image quality were evaluated; care factors were summarized. Results There were significant differences in liver perfusion between Omnipaque 350 and Ultravist 370 contrast agent (P <0. 01). The hepatic artery CT peak value with 4 ml/s injection rate was significantly smaller than that with 6ml/s. Conclusions The hepatic artery CT peak value and vascular reconstruction image quality with high injection rate and contrast agent concentration in liver perfusion examination by 320 slices CT arrive 3 class or above. Good nursing measures will improve image quality and success rate of examination. Key words: CT; Liver perfusion; CT peak; Injection rate; Concentrations of contrast agent; Nursing