Henna Tattoos Research Articles

LIBS for the detection of lead in ready to use henna paste and nutrients in fresh henna leaves and cultivated soils

Commercially available ready to use henna paste, procured from the local market in Pakistan were tested to find the level of hazardous element, lead present in the samples, using Laser Induced Breakdown Spectroscopy (LIBS). The source of lead in the ready to use henna is from the artificial chemical additives in henna paste, added to enhance the color and also to speed up the dying of the henna tattoo in hand, feet, and body. Henna tattooing is an inevitable practice in the social events in the cultures of the Indian subcontinent and the Arabian Peninsula. The frequent reckless use of henna, particularly lavishly administering it to the children is quite dangerous, although the level of lead found in the commercially available ready to use henna samples are within the permissible safe level. For the sensitive detection and quantification of lead, various experimental and plasma parameters in the LIBS detection system were optimized and the system was calibrated. The quantitative analysis of lead, carried out in five different samples of ready to use henna, using LIBS revealed that the concentration of lead in these samples is between 5.5 ± 0.2 mg Kg−1 and 16.0 ± 0.2 mg Kg−1. Moreover, LIBS elemental analysis of natural henna leaves, cultivated in Sindh and Punjab regions of Pakistan, and also the soils taken from the locations of cultivation were carried out and a correlation in the elemental compositions between the henna leaves and the cultivated soil in the proximity were studied. The quantitative analysis of LIBS results were validated by atomic absorption spectroscopy (AAS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES).

Are henna tattoos harmless? Report of clinical cases

The black henna tattoo has been of use in Mexico for a long time. It has become popular in the last few years mostly in young children and adolescents. The traditional henna tattoo is obtained from the Lawsonia alba and inermis plant. However, para-phenylenediamine (PPDA) is added to this so it can last longer, look darker, and look like a permanent tattoo. This increases its allergic potential since it has no sanitary regulation of the concentration of PPDA that it is used. We present three cases of children with contact allergic dermatitis caused by black henna which resolved with low potency topical steroids. The objective of this is to educate contact health-care professionals to be aware of this adverse effect on black henna and also to educate patients and patients' families about the risk of applying it, since it can have severe allergic reactions.

Allergic Contact Dermatitis to Henna Tattoo with Negative Patch to p-phenylenediamine (PPD)

Allergic Contact Dermatitis to Henna Tattoo with Negative Patch to p-phenylenediamine (PPD)

ALLERGIC CONTACT DERMATITIS NOT DUE TO P-PHENYLENEDIAMINE (PPD) IN A HENNA TATTOO

Introduction Henna tattoo application is a common practice in Eastern cultures but has become increasingly popular in the US as a form of temporary body art. The henna dyeing agent itself rarely leads to skin sensitization. The majority of cases of allergic contact dermatitis are associated with coloring agents, usually p-phenylenediamine (PPD). PPD is added to henna dye to make it darker and speed up the dyeing process. Case Description A 31-year-old healthy, Indian female who was also a first-year Allergy fellow, presented with intense itching and burning three days after the application (Panel B) of a temporary henna tattoo for her wedding (Panel A, original application). She began to have blistering on the fourth day (Panel C). After treatment with oral steroids, the dermatitis resolved without post-inflammatory hyperpigmentation. She previously had henna tattoos without reaction. T.R.U.E. TEST patch testing to 35 allergens (including PPD) and a negative control was negative. Additionally, patch testing to natural henna was negative also. Discussion In most cases, henna reactions are caused by chemical coloring additives, most commonly, PPD. Substances such as lemon, oil, vinegar, eucalyptus oil, and essential oils can also added to obtain different shades. Our patient's henna artist added a lemongrass-scented essential oil to the henna mixture. The essential oil used (unable to obtain for testing) may have been the sensitizing allergen. Allergists should be aware that the most common allergen may not be the culprit agent and more extensive testing may be required.

Allergic Contact Dermatitis Due to Paraphenylenediamine: An Update

Paraphenylenediamine (PPD) is an amine that is mainly used as an ingredient in hair dyes and henna tattoos. The incidence of allergic contact dermatitis to PPD is increasing, particularly in younger patients. In this article, we review the main sources of PPD and the substances with which it can interact and present a practical algorithm for diagnosing and treating suspected cases of PPD allergy.

Actualización en la dermatitis de contacto alérgica por parafenilendiamina

Paraphenylenediamine (PPD) is an amine that is mainly used as an ingredient in hair dyes and henna tattoos. The incidence of allergic contact dermatitis to PPD is increasing, particularly in younger patients. In this article, we review the main sources of PPD and the substances with which it can interact and present a practical algorithm for diagnosing and treating suspected cases of PPD allergy.

Two cases of henna tattoo⁃induced allergic contact dermatitis

Two cases of henna tattoo⁃induced allergic contact dermatitis

Factors associated with p-phenylenediamine sensitization: data from the Information Network of Departments of Dermatology, 2008-2013.

Risk factors for p-phenylenediamine (PPD) sensitization include the use of hair dyes, the application of temporary black henna tattoos, working as a hairdresser, and, possibly, exposure to hair dye pretests. To quantify the impact of these (putative) risk factors on PPD sensitization. Six items related to PPD exposure were added to the routine Information Network of Departments of Dermatology questionnaire from 2008 to 2013. A retrospective analysis of data from 4314 patients tested with PPD 1% pet. was conducted. Of the PPD-positive patients (n = 271), 80% had their hair dyed, and, of these, 57% subsequently developed scalp dermatitis, whereas only 11% had had a henna tattoo. The self-administrated pretest with hair dye was performed by only a few patients, precluding a more detailed analysis. Hair dyeing [odds ratio (OR) 6.0; 95% confidence interval (CI): 3.9-9.4], henna tattoos (OR 2.4; 95%CI: 1.5-3.7) and being a hairdresser (OR 2.1; 95%CI: 1.3-3.2) increased the risk of PPD sensitization. Neither dyeing of own hair nor application of a temporary henna tattoo seemed to affect PPD sensitization in hairdressers. p-Aminoaryl compounds more often gave positive reactions in patients with henna tattoo. Hair dyeing is the major risk factor for PPD sensitization in this clinical setting, and application of a temporary black henna tattoo may also lead to (strong) PPD sensitization.

Boiardo and Ariosto in Contemporary Sicilian Puppet Theater and the Tuscan-Emilian Epic Maggio

Boiardo and Ariosto in Contemporary Sicilian Puppet Theater and the Tuscan-Emilian Epic Maggio Jo Ann Cavallo Characters from medieval and Renaissance chivalric texts have been given tangible form through the centuries not only in operatic, melodramatic, theatrical and, more recently, cinematic adaptations, but also in popular performance traditions such as Sicilian puppet theater (opera dei pupi) and the folk operas (maggi epici) of the Tuscan-Emilian Apennines. This essay traces the reimagining of a sampling of non-Christian characters and non-European places from Matteo Maria Boiardo’s Orlando Innamorato and Ludovico Ariosto’s Orlando Furioso by a number of contemporary puppet theater and maggio companies. My contention is that an examination of select scenes available online, in conjunction with a close reading of the original episodes, may help students think more deeply about perennial issues addressed in the two poems—related to gender roles, the foreign and religious Other, violence, and political power—that continue to be relevant today.1 [End Page 48] Sicilian puppet theater No art form has devoted as sustained and elaborate attention to the Orlando Innamorato and the Orlando Furioso as Sicilian puppet theater.2 Although today we are past the time when Orlando and his fellow knights relived their adventures a puntate every evening in teatrini across Sicily and beyond, we can still encounter puppeteers, especially in Palermo, Catania, and Siracusa, who regularly present episodes from the two poems. The plays may be adapted for audiences who are no longer familiar with the original stories, but the puppeteers themselves have an intimate knowledge of their sources and make meaningful choices as they adapt narratives for the stage.3 Angelica The two most popular plays performed in Sicily today are the Innamorato’s battle between Rinaldo and Orlando for Angelica and the Furioso’s madness of Orlando. Since Angelica is central to both episodes, these plays offer ample material for a comparative examination of her character in contemporary Sicilian puppet theater. Regarding Angelica’s physical features, neither Boiardo nor Ariosto exoticize her beauty and a longstanding Italian and European artistic tradition has for the most part represented her with European features and dress. Most traditional Sicilian puppeteers have followed suit—with one difference. Although both Boiardo and Ariosto refer to Angelica as a blonde, matching Italian Renaissance standards for ideal beauty, most of the Angelicas I have encountered when attending puppet plays and visiting puppet museums in Sicily since 2000 have had dark brown hair, possibly reflecting the more Mediterranean colors of Sicilian women.4 When I asked members of the traditional public to [End Page 49] describe Angelica, the reply was invariably that she was beautiful, tout court. Nor did her clothing set her apart as a foreigner. As the puppeteer Enzo Rossi explained, the traditional puppeteer’s wife simply made sure that Angelica had the most elaborate and exquisite outfit.5 One can also find exceptions to the general Europeanization of Angelica. The puppeteer Enzo Mancuso (Associazione Carlo Magno, Palermo) remembers that his uncle constructed a brown-toned Angelica, remarking: “Questa è una Angelica che mio zio ha visto sempre così, ha visto sempre mulatta perché giustamente è indiana.”6 The puppeteer Alfredo Mauceri (Compagnia dei Pupari Vaccaro-Mauceri, Siracusa) decided to render more visible Angelica’s foreign status initially by constructing an Indian Angelica—complete with a bindi, nose earring, and henna tattoo—and subsequently by dressing her in Middle Eastern garb that, as the puppeteer himself explains, “non è certo un abito tipico del Catai, ma ciò consente di distinguerla dagli abiti europei indossati dalle altre dame.”7 In addition to giving Angelica a physical form, puppeteers also develop her character through action and dialogue. The question my students have found most compelling has been whether the plays pick up on and accentuate her more dangerous and seductive connotations or whether they instead bring her more in line with traditional precepts for female conduct. Most Sicilian puppet plays I’ve been able to film tend to temper her more compromising moments, perhaps to avoid any behavior that would be considered outside accepted norms. Regarding, for example, the staging of the Innamorato’s opening scene in Charlemagne’s court, the puppeteer Salvatore Oliveri (Compagnia...

The Case With Contact Dermatitis And Cellulite After The Second Time Temporary Henna Tattoos

The Case With Contact Dermatitis And Cellulite After The Second Time Temporary Henna Tattoos

Contact Dermatitis Due To A Henna Tattoo: A Case Report

Allergic contact dermatitis is a late hypersensitivity reaction caused after the skin is exposed to a specific allergen. Temporary henna tattoos have become very popular in children and adults.The organic compound, p-phenylendiamine (PPD) is frequently added to the henna pigment to make it darker. A 13 year old patient with a personal history of asthma and allergic rhinitis with immunotherapy for sensitization to Dermatophagoides farinae and Dermatophagoides pteronyssinus who presented since once week prior to consultation,skin lesions consisting of pruritic erythematous papules on his right forearm 3 days after applying a henna tattoo. During the physical examination, the skin lesions were marked in the shape and definition of the tattoo and mild desquamation was observed. Skin patch tests (Bial Aristegui Standard Contact Dermatitis Panel, Spain) were applied with positive results to p-phenylendiamine 1% with 2++ (strong positive reaction with vesicular erythema, infiltration and papules) in the first reading 48 hours later. The second reading was performed upon 96 hours of applying the tests, finding a 3 +++ (extreme positive reaction with intense erythema and infiltration, coalescing vesicles, bullous reaction) result and confirming it 7 days later. The patient was treated with a medium potency topical corticosteroid, mometasone 0.1% and cetirizine 10 mg twice a day for 7 days showing resolution of his skin lesions and symptoms. Temporary henna tattoos have been associated with a variety of inflammatory skin reactions.

Para-phenylenediamine allergic contact dermatitis due to henna tattoos in a child and adolescent population

Abstract Introduction Henna tattoos are a very common practice in the adolescent population. Henna is very often admixed with para-phenylenediamine (PPDA) to improve the appearance of the tattoo. PPDA is a potent allergen, and is a frequent cause of allergic contact dermatitis (ACD). Material and method A study was conducted on the results of 726 consecutive children who had been patch tested in the University General Hospital Consortium of Valencia between 1980 and 2015. Results Almost half (49.7%; (361 cases) of the children had one or more positive patch test findings, with 4.7% (34) being allergic to PPDA. Mean age of patients allergic to PPDA was 12.4 years, and 44.2% were male. There were 2 cases (5.9%) of atopic dermatitis. Of the positive reactions, 73.5% were considered to be current clinically relevant. The sensitisation origin was a Henna tattoo in 50% of cases. Conclusion PPDA sensitisation is relatively common in the child and adolescent population. The most frequent origin is the performing of Henna tattoos adulterated with PPDA. Adolescents are at the higher risk of developing ACD due to Henna tattoos. Henna tattooing should be strongly discouraged in children.

The T Cell Response to the Contact Sensitizer Paraphenylenediamine Is Characterized by a Polyclonal Diverse Repertoire of Antigen-Specific Receptors

Paraphenylenediamine (PPD) is a common component of hair dyes and black henna tattoos and can cause skin sensitization and allergic contact dermatitis (ACD). The cutaneous inflammatory reaction associated with ACD is driven by both CD4+ and CD8+ T cells. However, the characteristics of such responses with respect to clonal breadth and magnitude are poorly defined. In this study, we have characterized the in vitro recall response of peripheral blood T cells prepared from PPD-allergic individuals to a PPD–human serum albumin (HSA) conjugate (PPD–HSA). Quantitative high throughput sequencing was used to characterize the changes in the repertoire of T cell receptor (TCR) α and β genes after exposure to antigen in vitro. The PPD conjugate induced expansion of T cells carrying selected TCRs, with around 800 sequences (around 1%) being 8 or more times as abundant after culture than before. The expanded sequences showed strong skewing of V and J usage, consistent with an antigen-driven clonal expansion. The complementarity-determining region 3 sequences of the expanded TCRs could be grouped into several families of related amino acid sequence, but the overall diversity of the expanded sample was not much less than that of a random sample of the same size. The results suggest a model in which PPD–HSA conjugate stimulates a broad diversity of TCRs, with a wide range of stimulation strengths, which manifest as different degrees of in vitro expansion.

Henna Tattoos and Present Day Dangers

Henna Tattoos and Present Day Dangers

Para-phenylenediamine allergy: current perspectives on diagnosis and management.

Para-phenylenediamine (PPD) is the commonest and most well-known component of hair dyes. Oxidative hair dyes and dark henna temporary tattoos contain PPD. Individuals may be sensitized to PPD by temporary henna tattooing in addition to dyeing their hair. PPD allergy can cause severe reactions and may result in complications. In recent years, frequency of positive patch test reactions to PPD has been increasing. Cross-sensitization to other contact allergens may occur, in particular to other hair dye components. Hairdressers are at a high risk for PPD allergy and require counseling regarding techniques to minimize exposure and protective measures while handling hair dye. We focus this review on the current perspectives of diagnosis and management of PPD allergy.

Temporary Henna Tattooing: a Case of Contact Dermatitis

A case of a temporary henna tattoo is presented in which red henna containing p-phenylenediamine (PPD) caused a severe adverse reaction. The eruption began three days after its application and patient experienced itching and mild pain at the tattoo site along with an erythematous, papular lesion. The topic is discussed with pictures of this eruption.

Monitoring of Lawsone, p-phenylenediamine and heavy metals in commercial temporary black henna tattoos sold in Turkey.

Henna has a very low allergic potential, and severe allergenic contact dermatitis is mainly caused by p-phenylenediamine (PPD), which is added to temporary black 'henna tattoos', and potentially also by some heavy metals. To determine the presence of, and quantify, Lawsone, PPD and heavy metal contaminants (cobalt, nickel, lead, and chromium) in commercial temporary black henna tattoo mixtures (n = 25) sold in Turkey. Lawsone and PPD concentrations were analysed with high-performance liquid chromatography, and heavy metal quantification was performed with inductively coupled plasma mass spectrometry. PPD was found in all 25 black henna tattoo samples purchased from tattoo shops; levels varied between 3.37% and 51.6%. Lawsone was detected (0.002-88.2%) in 21 of the 25 temporary black henna tattoo samples analysed. Heavy metal contaminant levels were 0.44-3.11 ppm for Co, 1.13-2.20 ppm for Ni, 1.59-17.7 ppm for Pb, and 35.0-76.9 ppm for Cr. Our results suggest that commercial temporary black henna mixtures containing PPD levels up to 51.6% pose a risk of contact sensitization and severe allergic contact dermatitis among users. It is important to identify both the additives and metallic contaminants of black henna tattoo products; the significance of metal contaminants has still to be assessed.

Sensitization and Clinically Relevant Allergy to Hair Dyes and Clothes from Black Henna Tattoos: Do People Know the Risk? An Uncommon Serious Case and a Review of the Literature

Henna (Lawsonia inermis L.) tattooing has been used in Egypt and India since ancient times. Today this temporary body art is becoming increasingly popular among young people. Various chemicals are added to henna to darken and enhance the definition of tattoos, especially para-phenylenediamine (PPD), which is a strong sensitizer known to cause cross sensitive reactions to azoic dyes and other para-amino compounds. We present the case of an 18-year-old girl who became clinically sensitive to textile dyes after having showed a serious reaction both to her first hair dying when she was 16 years old and following the application of a temporary henna tattoo when she was a kid. The evidence from our literature review showed 33 cases of manifest sensitization to hair dye and only one of observable contact allergy to both hair and textile dyes from henna tattoos. The sensitization of children may have long-life lasting consequences, because of cross-reaction to dyes and other chemicals contained in hair colourants, clothes and drugs. Since tattoos are very popular and globalization has increased the circulation of unauthorized products we point out the need for informative campaigns about the risk of sensitization caused by temporary tattoos.

Dermatitis alérgica de contacto a parafenilendiamina por tatuajes con henna en población pediátrica

IntroductionHenna tattoos are a very common practice in the adolescent population. Henna is very often admixed with para-phenylenediamine (PPDA) to improve the appearance of the tattoo. PPDA is a potent allergen, and is a frequent cause of allergic contact dermatitis (ACD). Material and methodA study was conducted on the results of 726 consecutive children who had been patch tested in the University General Hospital Consortium of Valencia between 1980 and 2015. ResultsAlmost half (49.7%; (361 cases) of the children had one or more positive patch test findings, with 4.7% (34) being allergic to PPDA. Mean age of patients allergic to PPDA was 12.4 years, and 44.2% were male. There were 2 cases (5.9%) of atopic dermatitis. Of the positive reactions, 73.5% were considered to be current clinically relevant. The sensitisation origin was a Henna tattoo in 50% of cases. ConclusionPPDA sensitisation is relatively common in the child and adolescent population. The most frequent origin is the performing of Henna tattoos adulterated with PPDA. Adolescents are at the higher risk of developing ACD due to Henna tattoos. Henna tattooing should be strongly discouraged in children.

Prevalence of Contact Allergy to p-Phenylenediamine in the European General Population

Population-based studies on contact allergy to p-phenylenediamine (PPD) are scarce. A cross-sectional study was performed to assess the prevalence of contact allergy to PPD and its risk factors in the general population of 5 European countries. A total of 10,425 subjects were interviewed, and a random sample (n = 2,739) was patch tested to PPD. Overall, 5,286 individuals (50.9%) reported having used hair colorants at least once in their lifetime (78% female, 20% male), and 35% had used hair colorants during the last 12 months. Hair colorant avoidance because of any skin problem during the lifetime was reported by 6%. Black henna tattoos had been used by 5.5% during their lifetime. The prevalence of PPD contact allergy was 0.8% (95% confidence interval 0.6-1.0%), with no statistically significant association with gender or hair dye use. The prevalence of PPD in black henna tattoo users was 3.2% versus 0.6% in nonusers (P < 0.001). A clinically relevant positive patch test reaction to PPD related to hair coloring products was found in 0.1% (95% confidence interval 0.0-0.2%). A significant association with PPD contact allergy was observed for subjects who had black henna tattoos in their lifetime, with an age- and gender-adjusted odds ratio of 9.33 (95% confidence interval 3.45-25.26, P < 0.001). Black henna tattoos are an important risk factor for PPD contact allergy.