Hengduan Mountains Research Articles

Biphyly of the genus Wendlandia and establishment of a new tribe Clavistigmateae and a new genus Clavistigma of Rubiaceae from Asia

AbstractWendlandia, as currently circumscribed, is a genus of Rubiaceae comprising about 90 species mainly in tropical and subtropical Asia. By sampling species from all four series (Clavigerae, Euexsertae, Montigenae, Subinclusae) and all tribes in the subfamily Dialypetalanthoideae, we examined the monophyly of Wendlandia based on phylogenetic reconstruction using the nuclear ribosomal internal transcribed spacer and four plastid DNA regions. Wendlandia was resolved as biphyletic, consisting of two distantly related lineages that correspond to the monotypic series Clavigerae (Wendlandia pendula), sister to the Chinese endemic genus Trailliaedoxa, and a clade containing the remainder sampled members of Wendlandia. Based on the isolated phylogenetic position of W. pendula and its unique morphological characteristics compared to the recognized tribes of the Vanguerieae alliance, we herein erect a new monotypic genus, Clavistigma, and a new tribe, Clavistigmateae to accommodate the unique species. The secondary pollen presentation and the unique seed morphology were firstly described for the species. The divergence time between Clavistigma and Trailliaedoxa was dated back to 13.8 mya, coinciding with the climate change in northwestern Yunnan driven by the uplift Tibet Plateau and the river incision in the Hengduan Mountains during the Miocene and Late Pliocene.

The evolution of cropping structure in prehistoric Xizang

The origin and spread of agriculture facilitated a decline in human mobility and eventually led to a predominantly sedentary lifestyle globally, including on the Tibetan Plateau. Previous studies have proposed an evolution of prehistoric agriculture, from millet-based to barley-based farming. However, details regarding the process are vague. Here, we present diachronic changes in cropping structure from Xizang on the basis of a quantitative analysis of archaeobotanical remains from 12 sites located in southeastern Xizang. The advent of agriculture in Xizang began in the southeastern region around 4800 cal a BP and resulted in a quick spread of millet agriculture from the Hengduan Mountains to the Yarlung Zangbo River region. Subsequently, the introduction of barley and wheat in Xizang led to the transformation of millet-based farming into mixed farming after 3600 cal a BP. Eventually, around 3000 cal a BP, barley and wheat dominated across the entire Xizang with declining occurrences of millet. It took more than 600 years for barley and wheat to dominate in the Tibetan cropping system, which may reflect the time required for these exotic species to adapt physiologically to their new niche. In addition to the diachronic changes in crop farming, the ratio of barley to wheat and foxtail millet to broomcorn millet also varied at different elevations possibly due to local environmental variations and the crops’ physiological requirements.

The impact of extreme precipitation on water use efficiency along vertical vegetation belts in Hengduan Mountain during 2001 and 2020

In the context of climate change, extreme precipitation events are continuously increasing and impact the water‑carbon coupling of ecosystems. The vertical vegetation zonation, as a characteristic of mountain ecosystems, reflects the differences in vegetation response to climate change at different elevations. In this study, we used the water use efficiency (WUE) as an indicator to evaluate the water‑carbon relationship. By using MODIS data, we analyzed the spatiotemporal patterns of gross primary productivity (GPP), evapotranspiration (ET), and WUE from 2001 to 2020, as well as the responses of WUE to extreme wetness factor Number of precipitation days (R0.1), extreme dryness factor Consecutive dry days (CDD), and meteorological factors under the vertical vegetation zonation. Our results showed that annual GPP and ET displayed a significant increasing trend between 2001 and 2020, whereas WUE showed a weak decreasing trend. Spatially, GPP and WUE decreased with increasing elevation. Analyzing the WUE of mountainous ecosystems as a unified whole may not precisely capture the reactions of vegetation to severe rainfall occurrences. In fact, across different vegetation belts in mountainous areas, there exists a negative correlation between WUE and R0.1, and a positive correlation with CDD. In terms of meteorological factors, the temporal variation of GPP was primarily associated with vapor pressure deficit (VPD) and temperature (Ta), while those of ET was mainly related to soil water content (SWC). WUE was affected by a combination of meteorological factors and had a certain degree of variation between different altitude intervals. These findings contribute to a better understanding and prediction of the relationship between extreme rainfall climate and water‑carbon coupling in mountainous areas.

Effects of environment and genotype-by-environment interaction on phenotype of Rorippa elata (Brassicaceae), an endemic alpine plant in the Hengduan mountains

Abstract Global climate change poses a severe threat to mountain biodiversity. Phenotypic plasticity and local adaptation are two common strategies for alpine plant to cope with such change. They may facilitate organismal adaptation to contrasting environments, depending on the influences of the environment or genotype or their interacted effects. In this study, we use an endemic alpine plant (Rorippa elata) in the Hengduan mountains (HDM) to unravel its phenotypic basis of adaptation strategy and evaluate the relative contributions of environment and genotype to its phenotype. We transplanted 37 genotypes of R. elata into two common gardens across low and high elevations (2800 vs. 3800 m) during 2021–2022. Nine fitness-related traits were measured, including flowering probability and glucosinolates (GS) content. We estimated the environmental or genotypic contributions to the phenotype and identified the main environmental components. Our results revealed that both environment and genotype-by-environment interactions contributed to the phenotypes of R. elata. Latitudinal heterogeneity was identified as a key factor that explained 24% of the total phenotypic variation. In particular, genotypes of the northern HDM showed significantly higher plasticity in flowering probability than those of the southern HDM. Furthermore, within the southern HDM, GS content indicated local adaptation to herbivory stresses for R. elata genotypes along elevations. In conclusion, our results suggest that R. elata may have adapted to the alpine environment through species-level plasticity or regional-level local adaptation. These processes were shaped by either complex topography or interactions between genotype and mountain environments. Our study provides empirical evidence on the adaptation of alpine plants.

Mother-reliant or self-reliant: the germination strategy of seeds in a species-rich alpine meadow is associated with the existence of pericarps.

Some plants germinate their seeds enclosed by a pericarp, whereas others lack the outer packaging. As a maternal tissue, the pericarp might impart seeds with different germination strategies. Plants in a community with different flowering times might separately disperse and germinate their seeds; therefore, flowering time can be considered as one manifestation of maternal effects on the offspring. The mass of the seed is another important factor influencing germination and represents the intrinsic resource of the seed that supports germination. Using seeds from a species-rich alpine meadow located in the Hengduan Mountains of China, a global biodiversity hotspot, we aimed to illustrate whether and how the type of seed (with or without a pericarp) modulates the interaction of flowering time and seed mass with germination. Seeds were germinated in generally favourable conditions, and the speed of germination [estimated by mean germination time (MGT)] was calculated. We quantified the maternal conditions by separation of flowering time for 67 species in the meadow, of which 31 produced seeds with pericarps and 36 yielded seeds without pericarps. We also weighed 100 seeds of each species to assess their mass. The MGT varied between the two types of seeds. For seeds with pericarps, MGT was associated with flowering time but not with seed mass. Plants with earlier flowering times in the meadow exhibited more rapid seed germination. For seeds without a pericarp, the MGT depended on seed mass, with smaller seeds germinating more rapidly than larger seeds. The distinct responses of germination to flowering time and seed mass observed in seeds with and without a pericarp suggest that germination strategies might be mother-reliant for seeds protected by pericarps but self-reliant for those without such protection. This new finding improves our understanding of seed germination by integrating ecologically mediated maternal conditions and inherent genetic properties.

Soil Hydrothermal Dynamics in the Hengduan Mountains of Southeast Tibet and Associated Influencing Factors

Soil water and soil temperature are important ecological factors and driving forces for ecosystem restoration and sustainable development, possessing great significance for climate modeling and prediction. The Hengduan Mountains in southeastern Tibet, China, are located in a climate-change-sensitive area, and the study of soil hydrothermal dynamics in this area is of great significance for local and global climatic change and water resource utilization. This study, based on the soil hydrothermal and meteorological data of the Hengduan Mountain area in Southeast Tibet, analyzes the dynamic change patterns of soil hydrothermal and meteorological factors and explores their influencing relationships. It was found that the dynamic change in soil water content affected by precipitation was “bimodal” type. Among the meteorological factors, soil water content has the strongest correlation with relative humidity. The intra-annual variation curve of soil temperature is similar to that of the atmospheric temperature, showing a “unimodal” type, and has the highest correlation with atmospheric temperature. Specifically, it takes 70 mm and 170 mm of precipitation to change the soil water content and soil temperature at the 150 cm depth. For every 20 °C change in atmospheric temperature, soil temperature above 150 cm changes by an average of 7.2 °C.

New species and new Chinese record of Hypocreaceae from China

Trichoderma clavaticapitatum sp. nov. is described and illustrated based on specimen collected from alpine Hengduan Mountains, Yunnan Province, China. The fungus is characterized by simple, clavate stromata with a capitate fertile part, flask-shaped, subglobose to oblong perithecia, and cylindrical asci containing 16 part-ascospores. Morphological characteristics and analyses of combined RPB2 and TEF1 DNA sequences support its placement in Trichoderma. Differences between the novel species and its close relatives are compared in detail. Additionally, Hypocreopsis rhododendri, a rare fungus, is reported for the first time from the country.

Geomorphic indicators of continental-scale landscape transience in the Hengduan Mountains, SE Tibet, China

Abstract. Landscapes are sculpted by a complex response of surface processes to external forcings, such as climate and tectonics. Several major river captures have been documented in the Hengduan Mountains, leading to the hypothesis that the region experiences exceptionally high rates of drainage reorganization driven by horizontal shortening and propagating uplift. Here we determine the prevalence, intensity, and spatial patterns of ongoing drainage reorganization in the Hengduan Mountains and evaluate the relative timescales of this transience by comparing drainage divide asymmetry for four geomorphic metrics that operate at different spatial and temporal scales. Specifically, we calculate the migration direction and the divide asymmetry index (DAI) for drainage divides using catchment-restricted topographic relief (CRR), hillslope gradient (HSG), normalized channel steepness (ksn), and normalized channel distance (χ). ksn and χ are both precipitation-corrected to account for the strong precipitation gradient across the region. The different spatial scales of these geomorphic metrics allow us to establish the relative timescales of observed landscape transience in the Hengduan Mountains, where local-scale metrics measure short-term change and integral quantities measure long-term disequilibrium. We find a high incidence of strongly asymmetric divides in all metrics across the Hengduan Mountain region. Although the magnitude of asymmetry varies significantly between metrics, possibly due to a combination of metric-specific thresholds and varying proxy relationships with erosion rate, a majority of divides agree on divide migration direction. Agreement in divide migration direction indicates an actively responding landscape when asymmetry is high and a state of quasi-equilibrium when asymmetry is low. Disagreements between the integral quantity, χ, and the other geomorphic metrics can be explained by different timescales of the underlying geomorphic processes, with χ reflecting a long-term response and CRR, HSG, and ksn capturing short-term perturbations to catchment structure. These perturbations include various transient mechanisms, such as differential tectonic uplift or erodibility, glacial alteration, and river captures. Our work confirms the high incidence of drainage reorganization across the Hengduan Mountains and highlights both transient and stable areas in the landscape with high resolution. We also offer valuable insights into the application of geomorphic metrics that can be generalized and applied to the study of landscape transience and drainage divide asymmetry in other settings.

Relation between soil glycerol dialkyl glycerol tetraethers and temperature in different climatic regions in China

AbstractTemperature variation in the past periods can provide valuable insights into the characteristics and driving mechanisms of current global warming and future changes. Branched glycerol dialkyl glycerol tetraethers (brGDGTs) are commonly used to as a temperature proxy to quantitatively reconstruct past temperature changes. However, it still remains unclear whether the relation between brGDGTs and temperature is influenced by other environmental factors, thereby affecting the accuracy of quantitative reconstructions. A comprehensive analysis of the relation between brGDGTs and temperature across various climate regions can greatly enhance the accuracy of reconstructing past temperature changes. Here, we investigate the distribution patterns of soil brGDGTs using 28 new topsoils collected from altitudes ranging from 2100 to 4000 m.a.s.l. in the Hengduan Mountains in the southeastern margin of the Tibetan Plateau (TP), compiled previously published soil brGDGT data in China. Our results reveal distinct distribution patterns of soil brGDGTs between humid and arid regions. Furthermore, we further investigated the correlation between brGDGT indices and various environmental factors from both altitudinal and regional scales. At the altitudinal scale, brGDGT indices exhibit a stronger correlation with mean annual air temperature (MAAT) in alkaline soils (pH > 7) than that in acidic soils (pH < 7). At the regional scale, this correlation is higher in alkaline soils than that in acidic soils as well and is stronger in humid soils (mean annual precipitation [MAP] > 800 mm) than that in semi‐humid and semi‐arid soil (400 < MAP < 800 mm) and arid soil (MAP < 400 mm). In addition, brGDGT‐inferred temperature is more affected by MAP in humid alkaline soils but less affected in humid acidic soils. Therefore, caution is needed to use suitable brGDGT index for palaeotemperature reconstructions in various climate regions.

The carrying capacity for vegetation of forest land across China: Near real-time monitoring and short-term forecasting based on satellite observation

Ecological restoration projects implemented over the past 20 years have substantially increased forest coverage in China, but the high tree mortality of new afforestation forest remains a challenging but unsolved problem. It is still not clear how much vegetation can be sustained by the forest lands with given water, energy and soil conditions, i.e., the carrying capacity for vegetation (CCV) of forest lands, which is the prerequisite for planning and implementing forest restoration projects. Here, we used a simplified method to evaluate the CCV across forest lands nationwide. Specifically, based on leaf area index (LAI) dataset, we use boosted regression tree and multiple linear regression model to analyze the CCV during 2001–2020 and 2021–2030 and explore the contribution of environmental factors. We find that there are three typical regions with lower CCV located in the Loess Plateau and the southern region of the Inner Mongolia Plateau, the Hengduan Mountain region, and the Tianshan Mountains. More importantly, the vegetation in the regions near the dry-wet climate transition zone show excess local carrying capacity for vegetation over the past two decades and they are more susceptible to potential climatic stress. In comparison, in the Greater Khingan Mountains and Hengduan Mountains, there is high potential to improve the forest growth. Temperature, precipitation and soil affects the CCV by shaping the vegetation in the optimal range. This indicates that more consideration should be given to restrictions of regional environmental constraints when planning afforestation and forest management. This study has important implications for guiding future forest scheme in China.

Southbound transmission of metallurgy: new excavations at Jicha in the Hengduan Mountains, Yunnan

AbstractJicha is a Bronze Age settlement located next to the upper Mekong River in the Hengduan Mountains of Yunnan, south-west China. Recent excavations have revealed details of successive occupation and copper-base industrial activity. The site's position and chronology provide evidence of north–south demographic movement and technological transmission along the eastern Qinghai-Tibet Plateau corridor.

Optical Properties and Possible Origins of Atmospheric Aerosols over LHAASO in the Eastern Margin of the Tibetan Plateau

The accuracy of cosmic ray observations by the Large High Altitude Air Shower Observatory Wide Field-of-View Cherenkov/Fluorescence Telescope Array (LHAASO-WFCTA) is influenced by variations in aerosols in the atmosphere. The solar photometer (CE318-T) is extensively utilized within the Aerosol Robotic Network as a highly precise and reliable instrument for aerosol measurements. With this CE318-T 23, 254 sets of valid data samples over 394 days from October 2020 to October 2022 at the LHAASO site were obtained. Data analysis revealed that the baseline Aerosol Optical Depth (AOD) and Ångström Exponent (AE) at 440–870 nm (AE440–870nm) of the aerosols were calculated to be 0.03 and 1.07, respectively, suggesting that the LHAASO site is among the most pristine regions on Earth. The seasonality of the mean AOD is in the order of spring > summer > autumn = winter. The monthly average maximum of AOD440nm occurred in April (0.11 ± 0.05) and the minimum was in December (0.03 ± 0.01). The monthly average of AE440–870nm exhibited slight variations. The seasonal characterization of aerosol types indicated that background aerosol predominated in autumn and winter, which is the optimal period for the absolute calibration of the WFCTA. Additionally, the diurnal daytime variations of AOD and AE across the four seasons are presented. Our analysis also indicates that the potential origins of aerosol over the LHAASO in four seasons were different and the atmospheric aerosols with higher AOD probably originate mainly from Northern Myanmar and Northeast India regions. These results are presented for the first time, providing a detailed analysis of aerosol seasonality and origins, which have not been thoroughly documented before in this region, also enriching the valuable materials on aerosol observation in the Hengduan Mountains and Tibetan Plateau.

Quantifying the relative importance of influencing factors on NPP in Hengduan Mountains of the Tibetan Plateau from 2002 to 2021: A Dominance Analysis

Net primary productivity (NPP) stands as a crucial indicator of ecosystem health and carbon cycling, shaped by a complex interplay of climate change and anthropogenic disturbances. Understanding the relative contributions of these factors is essential for informing strategies aimed at achieving carbon neutrality and peaking emissions. In this study, we used the Carnegie-Ames-Stanford-Approach (CASA) model to assess NPP dynamics in the ecologically fragile area of the middle Hengduan Mountains on the southeastern Tibetan Plateau (TPHM). Dominance Analysis (DA) was employed to quantify and visualize the relative importance of climate change and anthropogenic disturbances on NPP. Our findings revealed significant annual fluctuations in NPP, with 61.8% of the area experiencing improvement in NPP, although the overall trend was not statistically significant. Needleleaf forests and mixed forests below medium elevation (< 4000 m) suffered negative impacts from human activity intensity, while vegetation at medium-high elevation (4000–5000 m) showed positive effects. The area dominated by anthropogenic disturbances influencing NPP accounted for 49.16% of the study area, followed by combined effects (36.86%) and climatic factors (13.98%). Human activity intensity, particularly in nature reserves, emerged as the predominant driver of NPP in plateau regions, while climatic factors primarily drove NPP decline in river valleys and a few alpine mountains. Moreover, combined effects contributed to NPP enhancement in most regions. This study underscores the critical role of human activity intensity in shaping NPP dynamics in high-elevation regions of the Tibetan Plateau, providing valuable insights for ecological conservation and carbon stock enhancement efforts in ecologically fragile areas.

Substantial variation of the two‐dimensional spectrum of senescent leaf in subalpine forest along elevational gradients

AbstractLeaf senescence have shed light on its contribution to the recovery of nutrients and signaling of the transition from the active to the dormant stage in winter. The manner by which climatic and edaphic factors and plant phylogeny affect the senescent leaf economics spectrum (more mineral elements included) of different growth habits along elevational gradients in mountain ecosystems is highly uncertain. To fill this knowledge gap, we examined 15 representative subalpine forest species' senescent leaf functional traits (i.e., specific leaf area [SLA], leaf dry matter content [DMC], pH, carbon [C], nitrogen [N], phosphorus [P], potassium [K], calcium [Ca], sodium [Na], magnesium [Mg], manganese [Mn], aluminum [Al], iron [Fe], C:N, N:P, and C:P ratios) to determine the senescent leaf economics spectrum along elevational gradients in the Hengduan Mountains, China. Our results demonstrate that all the functional traits of senescent leaf showed strong correlations with one another, and K was observed as the hub for the leaf economics spectrum. Irrespective of the difference among growth habits (arbor vs. shrub), leaf habits (deciduous vs. evergreen), and leaf types (broad vs. needle), the variations in the two‐dimensional spectrum of the senescent leaf trait exhibited uniformly positive associations with elevation, primarily attributed to climatic drivers. However, the modulation of the senescent leaf economics spectrum by soil properties and plant phylogeny was surprisingly modest. Collectively, our results revealed that the resource trade‐off of senescent leaf showed a trend from acquisition to conservation along elevational gradients, which could broaden the plant economics spectrum to include senescent leaf.

Quantifying the impact of earthquakes and geological factors on spatial heterogeneity of debris-flow prone areas: A case study in the Hengduan Mountains

Quantifying the impact of earthquakes and geological factors on spatial heterogeneity of debris-flow prone areas: A case study in the Hengduan Mountains

Distribution Pattern of Species Richness of Endemic Genera in Mountainous Areas of Southwest China and Its Influencing Factors

China’s southwest mountainous region is one of the hotspots of biodiversity in the world. However, the study on the pattern of species richness distribution of endemic plants in this region and its influencing factors is still in the exploration stage. A clear regional spatial distribution pattern of plants and its influencing factors are very important for the protection and management of plant diversity in southwest China. In this study, 511 county-level regions in the southwest mountainous region were taken as examples to establish a plant species resource bank in the southwest mountainous region, and the distribution pattern of the proportion and abundance of endemic plant families and genera was analyzed based on 19 climate and environmental factors and 8 human disturbance factors in the southwest mountainous region. The geographical weighted regression model (GWR) was used to reveal the main factors affecting the spatial distribution of species. The results showed that: (1) A total of 4688 species investigated in the southwest mountain belong to 19 families and 32 genera. (2) The endemic species richness in the southwest mountainous region showed a significant uneven spatial pattern, mainly concentrated in the Heng Duan Mountain area, southwest Sichuan and northern Yunnan area, and the Ruoergai-Hongyuan area of West Sichuan Plateau. (3) The results of the GWR model showed that the diurnal mean temperature range, precipitation seasonality and distance from the main road had significant effects on species richness. The study on the distribution of endemic genera and their influencing factors in the southwest mountainous region has important implications for the exploration of the evolutionary history and species conservation of the mountain flora.

Analysis of Quantified Hillslope Erosion by Excess Topography in the Hengduan Mountain

Analysis of Quantified Hillslope Erosion by Excess Topography in the Hengduan Mountain

Bazzania calypogeoides (Marchantiophyta)—a peculiar new taxon from Eastern Hengduan (China)

Bazzania calypogeoides is described from several localities on the eastern edge of the Hengduan mountain system in Sino-Himalaya. The species is characterized by shallowly emarginate underleaves, strongly convex leaves, curved to the ventral side and with a very short sinus resulting in two unequal lobes. The position of the species was assessed using morphological and molecular genetic analyses. A morphologically similar species, B. wooroonooran, is known from Australasia and eastern Melanesia, but differs in several features. A description of the species, illustrations and discussions on the ecology and geography of the new taxon are provided.

Leaf ecological stoichiometry and anatomical structural adaptation mechanisms of Quercus sect. Heterobalanus in southeastern Qinghai–Tibet Plateau

BackgroundWith the dramatic uplift of the Qinghai–Tibet Plateau (QTP) and the increase in altitude in the Pliocene, the environment became dry and cold, thermophilous plants that originally inhabited ancient subtropical forest essentially disappeared. However, Quercus sect. Heterobalanus (QSH) have gradually become dominant or constructive species distributed on harsh sites in the Hengduan Mountains range in southeastern QTP, Southwest China. Ecological stoichiometry reveals the survival strategies plants adopt to adapt to changing environment by quantifying the proportions and relationships of elements in plants. Simultaneously, as the most sensitive organs of plants to their environment, the structure of leaves reflects of the long-term adaptability of plants to their surrounding environments. Therefore, ecological adaptation mechanisms related to ecological stoichiometry and leaf anatomical structure of QSH were explored. In this study, stoichiometric characteristics were determined by measuring leaf carbon (C), nitrogen (N), and phosphorus (P) contents, and morphological adaptations were determined by examining leaf anatomical traits with microscopy.ResultsDifferent QSH life forms and species had different nutrient allocation strategies. Leaves of QSH plants had higher C and P and lower N contents and higher N and lower P utilization efficiencies. According to an N: P ratio threshold, the growth of QSH species was limited by N, except that of Q. aquifolioides and Q. longispica, which was limited by both N and P. Although stoichiometric homeostasis of C, N, and P and C: N, C: P, and N: P ratios differed slightly across life forms and species, the overall degree of homeostasis was strong, with strictly homeostatic, homeostatic, and weakly homeostatic regulation. In addition, QSH leaves had compound epidermis, thick cuticle, developed palisade tissue and spongy tissue. However, leaves were relatively thin overall, possibly due to leaf leathering and lignification, which is strategy to resist stress from UV radiation, drought, and frost. Furthermore, contents of C, N, and P and stoichiometric ratios were significantly correlated with leaf anatomical traits.ConclusionsQSH adapt to the plateau environment by adjusting the content and utilization efficiencies of C, N, and P elements. Strong stoichiometric homeostasis of QSH was likely a strategy to mitigate nutrient limitation. The unique leaf structure of the compound epidermis, thick cuticle, well-developed palisade tissue and spongy tissue is another adaptive mechanism for QSH to survive in the plateau environment. The anatomical adaptations and nutrient utilization strategies of QSH may have coevolved during long-term succession over millions of years.

Adaptive thermogenesis of the brown adipose tissue in Apodemus chevrieri during cold acclimation

Abstract To investigate the effect of low temperature on body mass, thermogenic activity, and Uncoupling protein-1 (UCP1) content of brown adipose tissue (BAT) in Chevrier’s field mouse (Apodemus chevrieri), 50 healthy adult mice with similar body mass were used in our experiment. They were divided into five groups of ten individuals as follows: a control group (0 d), where animals were maintained under 25 ± 1°C and a 12L:12D (light:dark, lights on 08:00) photoperiod; the other groups were maintained under 5 ± 1°C and a 12L:12D photoperiod for 7 d, 14 d, 21 d and 28 d, respectively. At the end of the experiment, the changes in body mass, resting metabolic rate (RMR), nonshivering thermogenesis (NST), BAT mass, mitochondrial protein (MP) content, cytochrome c oxidase (COX) activity, and UCP1 content were measured. The results showed that compared with the control group (0 d), the body mass of the cold acclimation groups decreased significantly. In contrast, RMR, NST, BAT mass, MP content, COX activity, and UCP1 content of the cold acclimation group increased significantly. After cold acclimation for 28 days, RMR increased by 89.4%, NST increased by 50.4%, BAT mass increased by 44.6%, and UCP1 content increased by 36.0%. The ratio of (NST − RMR)/RMR was 1.03 after seven days and then dropped to 0.59 on day 21, and remained at a steady level thereafter. Evidently, NST was significantly positively correlated with BAT mass and UCP1 content. The results indicated that under continuous cold exposure, A. chevrieri could take appropriate measures to reduce body mass, increase RMR, induce BAT tissue proliferation, and unregulated UCP1 expression, thereby enhancing BAT thermogenic activity to cope with a low-temperature environment. In the early stage of cold acclimation, NST was dominant in thermogenesis, but as time continued, it reduced. This may represent a unique energy adaptation strategy of small rodents originating from the north and spreading southward toward the Hengduan Mountain regions.