Purpose: To study the hemostatic, anti-inflammatory and antibacterial effects of Sanqixiantao dressing. Methods: Sanqixiantao dressing was prepared by mixting with sanqixiiantao extract (8 %) with membrane-forming matrix (5:4:9:2 volume ratio of polyvinyl alcohol: Na CMC: gelatin: glycerol). Rats with local surface wounds were used to evaluate the effects of Sanqixiantao dressing on hemostatic time, wound healing time and infection rate. Serum levels of tumor necrosis factor (TNF)-α and interleukin (IL) 6 were determined. The anti-inflammatory and analgesic effects of Sanqixiantao extracts were assessed by dimethylbenzene-induced ear edema and acetic acid-induced abdominal writhing tests. In in vitro studies, the effect of the extract on blood clotting time, and its antibacterial activities against six pathogenic bacteria ( Escherichia coli , Staphylococous aureus , Pseudomonas aeruginosa , Staphylococcus epidermidis , Clostridium perfringens and Clostridium tetani ) were evaluated. Results: Sanqixiantao dressing significantly decreased hemostatic time ( p < 0.01), wound healing time ( p < 0.01) and infection rate (10 vs 100 %), when compared to control rats. Sanqixiantao extract significantly shortened blood clotting time in vitro ( p < 0.01), and showed antibacterial activities against E. coli (minimum inhibitory concentration, MIC: 0.4 mg/mL, MBC: 1.6 mg/mL), S. aureus (MIC: 0.8 mg/mL, minimum bacterial concentration, MBC: 3.2 mg/mL), P. aeruginosa (MIC: 0.8 mg/mL, MBC: 3.2 mg/mL), S. epidermidis (MIC: 1.6 mg/mL, MBC: 3.2 mg/mL). Besides, Sanqixiantao extracts (100, 200, 400 and 600 mg/kg) dose-dependently decreased dimethyl-benzene-induced ear edema and acetic acid-induced abdominal writhes in mice ( p < 0.05, p < 0.01, p < 0.01, p < 0.01). Conclusion: The results demonstrate that Sanqixiantao dressing has significant hemostatic, antiinflammatory and antibacterial effects in vivo and in vitro , and thus provide some support for the therapeutic application of Sanqixiantao dressing for treating skin wounds. Keywords: Sanqixiantao dressing, Acute skin wound, Hemostatic, Anti-inflammatory activity, Antibacterial effect, Herbal medicine