Hemoglobin Glutamer-200 Research Articles

Effects of haemoglobin-based oxygen carrier Hemoglobin glutamer-200 (bovine) on intestinal perfusion and oxygenation in a canine hypovolaemia model

The objective of this investigation was to study the effects of the first marketed haemoglobin-based oxygen carrier, Hemoglobin glutamer-200 (bovine) (Hb-200) (Oxyglobin) on splanchnic perfusion and oxygenation in a canine model of acute hypovolaemia. Twelve anaesthetized dogs [mean weight 30.8 (S.D. 1.4) kg] were instrumented for recordings of heart rate (HR), mean arterial pressure (MAP), central venous pressure (CVP), cardiac output and cranial mesenteric arterial (CMA) and venous blood flows (CMV). Total and plasma haemoglobin (Hb), oxygen content and saturation, lactate concentration, pH and blood gases were analysed in arterial, mixed venous and mesenteric venous blood samples. Measurements were made before (baseline) and after 1 h of haemorrhage, after which animals were resuscitated with either shed blood (controls) or Hb-200 until HR, MAP and CVP returned to prehaemorrhage levels. Recordings were repeated immediately and 3 h after termination of fluid resuscitation, after which organ specimens were obtained for microscopic examination. Haemorrhage (average 32 ml kg(-1)) reduced MAP to 50 mm Hg, increased HR and systemic vascular resistance (SVR), and was accompanied in both the systemic and the splanchnic circulation by significant decreases in blood flow, Hb content and oxygen delivery (DO2), and lactic acidosis. In controls, all variables recovered to baseline after isovolaemic resuscitation with shed blood. In dogs resuscitated with a small volume of Hb-200 (10 ml kg(-1)), HR, MAP, CVP and CMA and CMV blood flows returned to baseline. However, cardiac output, total Hb, oxygen content and systemic and mesenteric DO2 remained depressed while SVR increased further. Mesenteric and systemic acid-base status recovered in both groups, and there was no difference in microscopic tissue damage between groups. Thus, Hb-200 reconstituted splanchnic perfusion and oxidative metabolism in spite of pronounced systemic vasoconstriction and insufficient restoration of CO and DO2; it may improve diffusive oxygen transport in the microvasculature by virtue of haemodilution and its high efficiency in the uptake and release of oxygen.

Radiographic, biomechanical, and pathologic effects of hemoglobin glutamer-200 in dogs undergoing cemented total hip arthroplasty

Abstract Objective To determine whether use of hemoglobin glutamer-200 (bovine) as a partial blood volume replacement in dogs undergoing cemented total hip replacement caused any deleterious effects on the bone-cement or cement-prosthesis interface, exerted any deleterious effects on body organs, or caused any complications during the anesthetic, immediate recovery, or long-term recovery period. Animals 9 adult dogs. Methods Dogs were anesthetized, and 15% of the blood volume was removed. Simultaneously, lactated Ringer's solution was infused, and 6 dogs were given hemoglobin glutamer (1 g/kg of body weight, IV). Unilateral total hip replacement was performed. Limb use was assessed visually, and force-plate and radiographic evaluations were performed before, and 8 weeks after, surgery. Eight weeks after surgery, dogs were euthanatized, necropsies were performed, and prosthetic component pullout forces were determined. Results There were no significant differences between treated and control dogs in regard to biomechanical (visual assessment of gait, force-plate analysis, femoral and acetabular component pullout forces) and pathologic evaluations (physical examination, CBC, serum biochemical analyses, necropsy, and histologic evaluations). Radiographic signs of loosening of the femoral component were seen in 4 dogs treated with hemoglobin glutamer. Conclusions and Clinical Relevance Administration of hemoglobin glutamer as a blood substitute did not appear to have any deleterious effects in dogs undergoing total hip arthroplasty. The radiographic findings, which were discordant with the biomechanical results, merit further investigation. (Am J Vet Res 1999;60:1337–1340)

Effect of splenectomy on blood picture, blood volume, and plasma proteins in beagles.

ARTICLESEffect of splenectomy on blood picture, blood volume, and plasma proteins in beaglesRJ Dellenback, S Usami, S Chien, and MI GregersenRJ Dellenback, S Usami, S Chien, and MI GregersenPublished Online:01 Sep 1969https://doi.org/10.1152/ajplegacy.1969.217.3.891MoreSectionsPDF (2 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByA study on the correlation between spleen volume estimated via cavalieri principle on computed tomography images with basic hemogram and biochemical blood parametersAnatomy & Cell Biology, Vol. 55, No. 1Effects of low-volume hemoglobin glutamer-200 versus normal saline and arginine vasopressin resuscitation on systemic and skeletal muscle blood flow and oxygenation in a canine hemorrhagic shock model*Critical Care Medicine, Vol. 35, No. 9Effects of isovolemic resuscitation with hemoglobin-based oxygen carrier Hemoglobin glutamer-200 (bovine) on systemic and mesenteric perfusion and oxygenation in a canine model of hemorrhagic shock: a comparison with 6% hetastarch solution and shed bloodVeterinary Anaesthesia and Analgesia, Vol. 33, No. 6Splenic denervation worsens lipopolysaccharide-induced hypotension, hemoconcentration, and hypovolemiaPeter S. Andrew, and Susan Kaufman1 May 2001 | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 280, No. 5Effects of haemoglobin-based oxygen carrier Hemoglobin glutamer-200 (bovine) on intestinal perfusion and oxygenation in a canine hypovolaemia modelBritish Journal of Anaesthesia, Vol. 86, No. 5Transvascular fluid distribution of hyperoncotic Dextran solutionJournal of Anesthesia, Vol. 12, No. 1Plasma Concentrations of Thyroid Hormones in Dogs: Influence of Sampling Hour, Breed and Age7 July 2009 | Chronobiology International, Vol. 2, No. 2Relationship between apexcardiogram, left ventricular pressure and wall stressJournal of Biomedical Engineering, Vol. 4, No. 1Hematologic DisordersCirculatory Effects of Splenectomy in the Horse113 May 2010 | Zentralblatt für Veterinärmedizin Reihe A, Vol. 20, No. 6 More from this issue > Volume 217Issue 3September 1969Pages 891-897 Copyright & PermissionsCopyright © 1969 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1969.217.3.891PubMed5807717History Published online 1 September 1969 Published in print 1 September 1969 Metrics