You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology II1 Apr 2016MP61-09 EARLY DEVELOPMENT OF INTRAVESICAL REFLECTANCE SPECTROSCOPY FOR BLADDER TUMOR DETECTION AND STAGING Joel Slaton, Robert Hurst, Carole Davis, Pallavi Rajaputra, Youngjae You, Kenneth Bartels, and Daqing Piao Joel SlatonJoel Slaton More articles by this author , Robert HurstRobert Hurst More articles by this author , Carole DavisCarole Davis More articles by this author , Pallavi RajaputraPallavi Rajaputra More articles by this author , Youngjae YouYoungjae You More articles by this author , Kenneth BartelsKenneth Bartels More articles by this author , and Daqing PiaoDaqing Piao More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.883AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Cystoscopy provides direct imaging of macroscopic abnormalities of the urothelium within the visible spectrum. However, it is limited in diagnosing flat lesions such as CIS. The objective of this study is to develop the potential of single-fiber reflectance spectroscopy (SfRS) to discriminate neoplastic bladder tissue from normal tissue. METHODS Orthotopic AY-27 bladder tumors were developed in female Fisher rats (n=4) by urethral catheterized instillation of a suspension of GFP-labeled AY-27 cells into the urinary bladder. Two mice served as controls. At 7 days post-instillation, the animals were anesthetized and the bladder was bisected to expose the bladder surface. An optical fiber-probe of 320 µm in diameter tip was brought in contact with the bladder wall. SfRS measurements over a spectral range of 520-920 nm were taken from peripheral fat, normal bladder mucosa and tumor lesion. RESULTS Measurements were taken from sites on surface of normal (n-27 sites) and neoplastic tissue (n=81 sites). The neoplastic tissue demonstrated increased spectral reflectance as well as an increase of absorption, as evident by the notch of the oxyhemoglobin signature (~550nm) (Fig. 1). The spectrally-resolved total hemoglobin (HbT) increased significantly (p<0.001) in neoplastic bladder tissue (28.6% increase), indicating angiogenesis confirmed by microvessel staining. The spectrally-resolved MetHb fraction in HbT in neoplastic bladder tissue increased 30.2% (p<0.001) when compared to normal bladder tissue. The spectrally-resolved lipid fraction in the water-lipid body decreased significantly (p<0.001) in neoplastic bladder tissue, in agreement with the disruption of sub-urothelium tissue-matrix due to tumor invasion. CONCLUSIONS In this preclinical study using SfRS, bladder tumor was shown spectrally to exhibit an over-formation of MetHb within increased total hemoglobin content and a decreased lipid composition when compared to normal bladder tissue. As these spectrally-resolved changes are indicative of hemoglobin degradation within an angiogenic micro-vessel environment it is hypothesized that these physiological changes assessable by SfRS may be used as biomarkers of tissue malignancy © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e806 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Joel Slaton More articles by this author Robert Hurst More articles by this author Carole Davis More articles by this author Pallavi Rajaputra More articles by this author Youngjae You More articles by this author Kenneth Bartels More articles by this author Daqing Piao More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...