Hematological Data Research Articles

Comparing laboratory toxicity of selective intra-arterial radionuclide therapy for primary and metastatic liver tumors: resin versus glass microspheres.

To compare the acute (within 30days of treatment) laboratory toxicities of Yttrium-90 (Y-90) resin and glass microspheres. Selective intra-arterial radionuclide therapies (SIRTs) with Y-90 resin and glass microspheres were retrospectively reviewed. Liver-hematological data were collected at baseline and at 1week and 1month follow-up. The percentage change of laboratory data and the albumin-bilirubin (ALBI) score were calculated. A total of 219 SIRTs (n: 110 resin, n: 109 glass) from 177 patients were included. There was no difference in age, liver pathologies, extrahepatic disease, baseline liver function tests, and total blood counts between the two microsphere groups. Administered activity was higher in treatments with Y-90 glass microspheres (p < 0.001). An increase in serum liver enzymes was observed after treatment with both microspheres. The difference between the treatment groups was the higher percentage increase of AST and ALT at the first week following Y-90 glass treatment (p < 0.001). However, this situation was not observed after 1month. No difference in the percentage change of other laboratory parameters was found between two groups. The number of patients with an increase [resin n: 24 (24.7%) vs glass n: 26 (27.1%), p: 0.711) and decrease [resin n: 13 (13.4%) vs glass n: 8 (8.3%), p: 0.258] in the ALBI grade after SIRT was similar among groups. An increase in liver enzymes was observed in the early period after SIRT with both microspheres. No significant difference in liver and hematological data was detected during early follow-up between the two groups, except that the percentage increase of AST and ALT were higher at the first week in the Y-90 glass group, possibly due to higher administered activity.

Blood Morphology and Hematology of Adult Baikal Seals (Pusa sibirica Gmelin, 1788) Under Professional Care

Studying the blood cell morphology of marine mammals provides an opportunity to elucidate the physiological mechanisms of adaptive changes associated with the aquatic habitat that occur at the cellular level, as well as adaptations to changing environmental conditions and under various physiological and pathological processes. The Baikal seal [Pusa sibirica (family Phocidae)] is endemic to the freshwater Lake Baikal, but comprehensive hematology data are not available. We studied the morphological features of blood cells of twelve clinically normal, adult Baikal seals (n = 6 males, n = 6 females) from two oceanariums under professional care for eight years. The morphology of mature and immature erythrocytes and inclusions are described. The blood of Baikal seals is characterized by the presence of erythrocytes with a size of 8.2 ± 0.6 µm; Howell–Jolly bodies were rarely observed, the number of reticulocytes ranged from 4.1 to 93.1 × 109/L, and nucleated erythrocytes were absent. The morphological features of neutrophils, eosinophils, basophils, and platelets were described. Inter-individual and sex differences in the counts of basophils, platelets, red blood cells, and levels of hemoglobin, the mean corpuscular volume, and the mean corpuscular hemoglobin concentration were statistically observed. The results could be useful for Baikal seal veterinary care, immune response research, and comparative studies with other pinnipeds.

Nighttime systolic blood pressure a major factor of retinal vascular caliber changes in patients with newly diagnosed type 2 diabetes mellitus.

Changes in retinal vessel caliber are crucial for detecting early retinopathy, a significant cause of blindness in individuals with Diabetes Mellitus type 2 (T2DM). This study aims to evaluate the changes in retinal vessel caliber and identify factors associated with these changes in recently diagnosed T2DM patients. The study included newly diagnosed T2DM patients (within 6 months of diagnosis) who were free of antidiabetic treatment (except metformin) and matched individuals based on age and blood pressure (BP). Data collected included somatometric measurements, BP (office and 24-hour), hematological data, albuminuria (via 24-hour urine collections), ten-year atherosclerotic cardiovascular disease risk (ASCVD score), endothelial dysfunction (measured by Asymmetric Dimethylarginine, ADMA), retinal microvascular changes, assessed as central retinal arteriolar equivalent (CRAE), central retinal venular equivalent (CRVE), and arteriovenous ratio (AVR) using specialized software on non-mydriatic fundus photographs. The study involved 87 T2DM patients and 90 controls, aged 57±11 years. Key findings include no significant differences in CRAE, CRVE, and AVR between T2DM patients and controls. Age (p=0.019) and nighttime systolic BP (SBP) (p=0.002) were independent predictors of AVR. CRAE was independently associated with nighttime SBP (p=0.048). CRVE was independently associated with age (p=0.016), dipping (p=0.002), and smoking (p=0.018). In normotensive subjects, AVR was significantly lower in T2DM patients (p=0.035). The study concludes that increased nighttime SBP is a more critical factor than hyperglycemia in affecting retinal vascular caliber changes in newly diagnosed T2DM patients. This highlights the importance of managing nocturnal hypertension to prevent retinal damage in this patient population.

Preoperative anemia is associated with prolonged hospital stay and increased facility discharges after glioblastoma resection

BackgroundDespite numerous operative and non-operative treatment modalities, patients with glioblastoma (GBM) have a dismal prognosis. Identifying predictors of survival and recurrence is an essential strategy for guiding treatment decisions, and existing literature demonstrates associations between hematologic data and clinical outcomes in cancer patients. As such, we provide a novel analysis that examines associations between preoperative hematologic data and postoperative outcomes following GBM resection.MethodsWe performed a retrospective analysis of patients who underwent GBM resection from January 2016 to December 2020. Standard demographic and clinical variables were collected, including pre-operative complete blood count (CBC), and inferential analyses were performed to analyze associations between CBC parameters and postoperative outcomes.ResultsOne hundred and eighty nine (189) patients met inclusion criteria, with a mean age of 60.7 years. On multivariate regression analysis, controlling for age, gender, and performance status, we observed trends suggesting anemic patients may have longer lengths of stay (t statistic = 3.23, p = 0.0015) and higher rates of discharge to inpatient facilities [OR 3.01 (1.09–8.13), p = 0.029], though these associations did not reach statistical significance after correction for multiple comparisons (Bonferroni-corrected significance threshold p &amp;lt; 0.01).ConclusionPreoperative anemia may be a useful pre-operative predictor of postsurgical GBM outcomes. Further study is required to determine whether pre-operative hemoglobin optimization can improve postoperative clinical outcomes, and whether other hematologic and inflammatory markers are predictive of postoperative recovery and functional status.

Environmental PM2.5 Exposure: An Ignored Factor Associated with Blood Cadmium Level in Hemodialysis Patients.

The negative impacts of particulate matter with an aerodynamic diameter of 2.5μm or less (PM2.5) are well known. Patients undergoing maintenance hemodialysis (HD) have significantly higher blood cadmium levels (BCLs) than healthy individuals. As elemental cadmium can be found in the PM2.5 particle fraction, we conducted this study to assess the effect of environmental PM2.5 exposure and other clinical variables on BCLs in maintenance HD patients. This cross-sectional study included 754hD patients who had previously participated in a BCL study. Demographic, hematological, biochemical and dialysis-related data were collected for analysis. For each patient, the mean PM2.5 concentrations in the living environment during the previous 12 and 24 months were recorded and analyzed. Of all patients, the median BCL of was 0.36µg/L (range: 0.21, 0.79µg/L). The mean PM2.5 concentration was 28.45 ± 3.57 μg/m3 during the 12 months and 29.81 ± 3.47 μg/m3 during the 24 months, respectively. From a multivariate linear regression analysis, log BCL was positively associated with the mean PM2.5 concentration during the previous 12and 24 months. In addition, log BCL was positively associated with the number of days with PM2.5 concentrations above the standard level during the previous 12 and 24 months. Moreover, according to the tertiles of days with a daily mean PM2.5 concentration above the normal limit in the previous 24 months, patients with the highest exposure days exhibited a significantly higher BCL than those in the other two patient groups. Chronic environmental exposure to PM2.5 is significantly associated with BCLs in maintenance HD patients, and exposure to PM2.5-bound cadmium may contribute to the harmfuleffectsonhealth in this population. Further studies are needed to confirm these observations and to explore the underlying mechanisms.

The relationship between hypertension and COVID-19 vaccine in the long term and occupational evaluation

Introduction: Many cardiovascular complications, especially myocarditis and pericarditis, have been observed with vaccination. One of these cardiovascular complications is hypertension with a rate of 1-5%. Hypertension emerged in the acute period after vaccination and tended to be persistent in patients with advanced age and comorbid diseases. In this study, we aimed to examine the relationship between hematological, biochemical markers and demographic characteristics between healthy individuals who have never been vaccinated or vaccinated and patients with new- onset hypertension. Material and Method: Patients diagnosed with new-onset hypertension and healthy COVID-19 vaccinated, and non-COVID-19 vaccinated control patients of similar age and number were included in the study. The relationship between hematological, biochemical and demographic data between newly diagnosed hypertension patients and healthy COVID-19 vaccinated, and healthy non-vaccinated patients was examined. Result: 56.3% of newly diagnosed hypertension patients were female, 46.9% were primary school graduates, 37.5% were housewives, 81.3% had stress in their lives. Hypertension patients were older (p&lt;0.01), had lower hemoglobin levels (p=0.05) and higher LDL-C levels (p&lt;0.01) than healthy unvaccinated patients. Conclusion: Although it is seen that the cause of hypertension cannot be attributed solely to the vaccine, since the hypertensive group was older, had a higher body mass index, hyperglycemia and hyperlipidemia compared to the healthy group, and the number of patients in the study was small, but being a housewife is the most important occupational group and stress is an important trigger. The majority of vaccinees were primary school graduates. Anemia and LDL elevation were found in hypertensive and vaccinated patients. To the best of our knowledge, it is one of the first studies to examine LDL elevation in vaccinated patients and the relationship between long- term newly diagnosed hypertension and vaccination, although confounding factors cannot be excluded.

Has the UK lost its position as a destination for world-leading clinical research? A comparative analysis of haematological cancer clinical trials performance before Brexit

ObjectivesTo understand the competitive position of the UK in comparison to Europe and the USA for haematological cancer clinical research.DesignUsing commercially available databases, clinical trial numbers, their effectiveness and publication...

Aeromonas hydrophila dose and post-immunostimulation period as immunomodulatory effect indicator on Nile tilapia (Oreochromis niloticus)

In fish challenge tests, there is no standardization in the concentration and analysis period of parameters. This study investigates the immunomodulatory and cytotoxic effects of A. hydrophila dose and postimmunostimulation periods on Nile tilapia. Four level of bacterial doses (½, ¼, ⅛, and 0 LD50) were administered, and immune parameters, hematological data, and erythrocyte abnormalities were analyzed at 3, 7, 10, and 14 days post-infection (dpi). The highest respiratory burst, total globulins, and lysozyme activity were detected at 7 dpi. Considering the bacteria doses, the lysozyme activity was higher in the ⅛ LD50 and ¼ LD50 of A. hydrophila. Erythrocytes, hematocrit, hemoglobin, leukocytes, lymphocytes, neutrophils, and monocytes counts remained unchanged. Ten days post-injection, the control group exhibited reduced abnormalities, showing a notable distinction from the ⅛ LD50 and ¼ LD50 treatments, particularly in hooked and notched nuclei abnormalities. Mortality rates were higher in infected groups, peaking at different times with the highest in ½ LD50. For evaluating Nile tilapia immunity post A. hydrophila infection, it is recommended ¼ LD50 dose and blood collection at 7 and 10 dpi for immunological parameters and abnormalities in Nile tilapia erythrocytes, respectively.

EFFECT OF DIPOTASSIUM ETHYLENEDIAMINETETRAACETIC ACID AND LITHIUM HEPARIN ANTICOAGULANT ON HEMATOLOGIC VALUES IN THE BEARDED DRAGON (POGONA VITTICEPS) IN A CLINICAL SETTING.

Anticoagulant choice can have significant effect on complete blood counts of reptiles and has not been reported in bearded dragons (Pogona vitticeps). Whole blood was collected from 14 captive individuals and aliquoted into separate blood tubes containing dipotassium ethylenediaminetetraacetic acid (EDTA) and lithium heparin. Samples were shipped and processed within 24 h. A CBC, including a WBC count using a commercial phloxine method, was performed on blood from both tubes. A WBC estimate, WBC differential, and morphologic review were performed on blood smears made from the anticoagulated blood, as well as on a direct blood smear made from anticoagulant-free blood. Some CBC values were significantly different between the EDTA and lithium heparin samples. Hematologic data generated from direct blood smears were more similar to data from EDTA samples than to data from lithium heparin samples. Additionally, multiple lithium heparin samples had poor phloxine stain uptake and leukocyte clumping, so that many WBC parameters were altered or unreportable. These results support EDTA as an appropriate anticoagulant for bearded dragon hematologic evaluation and suggest it may be superior to heparin for some individuals in generating a WBC count using a commercial phloxine method.

Prognostic value of immunotherapy in advanced NSCLC based on baseline and dynamic changes in HALP.

Immune checkpoint inhibitors (ICIs) enhance the tumor-killing ability of T-cells in non-small cell lung cancer (NSCLC), improving overall survival (OS) and revolutionizing treatment for advanced stages. However, challenges remain, such as low response rates and the lack of effective markers for selecting candidates. This study evaluated the impact of hemoglobin, albumin, and platelet (HALP), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) on the efficacy of immunotherapy and survival outcomes in advanced NSCLC. Additionally, it aimed to develop a nomogram based on these parameters. Clinical and hematological data from NSCLC patients who received immunotherapy were analyzed. Efficacy was assessed using the immune Response Evaluation Criteria in Solid Tumors (iRECIST), and progression-free survival (PFS) and OS were evaluated. Prediction models incorporated baseline and post-treatment HALP, NLR, and PLR values. The 203 patients had a median follow-up of 16 months, a median PFS (mPFS) of seven months (6.0-8.0), while the median OS (mOS) was not reached (24.0-not available). Pretreatment PLR (PLR0) was associated with a higher disease control rate (DCR) (odds ratio [OR] = 0.258), while initial immunotherapy and NLR after four treatment cycles (NLR4C) significantly improved the objective response rate (ORR). Cox regression analysis showed that pretreatment HALP (HALP0), HALP after four cycles of treatment (HALP4C), and pretreatment NLR (NLR0) significantly impacted PFS. Additionally, HALP0, NLR0, and PLR after four treatment cycles (PLR4C) were associated with OS. The C-indices for PFS and OS were 0.823 and 0.878, respectively, indicating good predictive accuracy. HALP, NLR, and PLR at various time points effectively predicted immunotherapy response in advanced NSCLC patients, with low HALP combined with high NLR and PLR indicating a poor prognosis. These findings could serve as the basis for stratified randomized controlled trials (RCTs) in the future.

Hematological and Biochemical Characteristics Associated with Cytogenetic Findern Alterations in Adult Patients with Acute Lymphoblastic Leukemia (ALL) from the Northern Region of Brazil

Acute lymphoblastic leukemia (ALL) is an aggressive neoplasm derived from B and/or T cell lineage (B-ALL; T-ALL). For the first time, this study describes, cytogenetically, the karyotypic alterations in adults with ALL in the northern region of Brazil and their relationship with hematological and biochemical characteristics. Through banding analyses, immunophenotyping, as well as hematological and biochemical examination data obtained directly from patients’ records, we found that chromosome 21 aneuploidy was the most frequent. The cytogenetic structural alterations observed with the highest incidence among the patients were: t(9;22), t(4;11), t(1;19), del(6q), and del(9p). In patients presenting with chromosome alterations, we verified that patients with t(4;11) have elevated red blood cell levels and patients with del(9p) presented with distinct and high values of hematological parameters compared to other patients. Regarding biochemical alterations, we observed that patients with translocations (4;11) and del(6q) presented with elevated urea levels compared to other patients, highlighting its relationship to kidney changes and patient prognosis. Thus, our study highlights that variations in hematological and biochemical data are associated with specific cytogenetic changes and other factors, which may impact the prognosis of adult patients with ALL.

Advanced Anemia Classification Using Comprehensive Hematological Profiles and Explainable Machine Learning Approaches

Anemia is a common health issue with serious clinical effects, making timely and accurate diagnosis essential to prevent complications. This study explores the use of machine learning (ML) methods to classify anemia and its subtypes using detailed hematological data. Six ML models were tested: Gradient Boosting, Random Forest, Naive Bayes, Logistic Regression, Support Vector Machine, and K-Nearest Neighbors. The dataset was preprocessed using feature standardization and the Synthetic Minority Oversampling Technique (SMOTE) to address class imbalance. Gradient Boosting delivered the highest accuracy, sensitivity, and F1-score, establishing itself as the top-performing model. SHapley Additive exPlanations (SHAP) analysis was applied to enhance model interpretability, identifying key predictive features. This study highlights the potential of explainable ML to develop efficient, accurate, and scalable tools for anemia diagnosis, fostering improved healthcare outcomes globally.

Exploring hematological diversity, a comprehensive study of CBC variations among the population of Tehsil Kot-Momin Sargodha

Objectives: To ascertain the diversity and patterns observed in the complete blood count within the population of Tehsil Kot-Momin , Sargodha. Study Design: Observational Cross Sectional Study Place of Study: Rai Medical College,Sargodha Materials &amp; Methods: A comprehensive collection of 401 CBC samples was meticoulously acquired from individuals at Medina Medical Complex in Kot-Momin ,Sargodha, subsequent to securing verbal informed consent. Subsequent research activities were conducted at Rai Medical College,Sargodha, post-recipt of sanction from the institutional ethical review committee. The time frame for data accumulation extended from june 2023 through August 2023 and the results were analyzed on SPSS Ver 27. Results: The study aimed to explore gender differences in hematological parameters among a sample of 401 individuals. Key findings indicated significant gender-based variations in hemoglobin (p &lt; 0.001), total RBC count ( p&lt; 0.001), hematocrit (p &lt;0.001) , platlelt count (p=0.008), and mean platelet volume (MPV) (p=0.033). In this investigation, male subjects were observed to possess higher mean concentration of hemoplobin , total erythrocyte count and hematocrit levels relative to their female counterparts, who demonstrated an augmented mean thrombocyte count. Significantly, the mean thrombocyte volume (MTV)-a proxy for platelet volume - was markedly distinct between genders, alluding to potential differences in thrombocyte function or production. Conversly, the study revealed no statistically significant disparities in parameters such as leukocyte count (WBC) , mean corpuscular volume ( MCV), mean corpuscular hemoglobin (MCH) , mean corpuscular hemoglobin concentration (MCHC), and proportions of hematocrit (PCT), as well as neutrophil,lymphocyte ,monocyte, eosinophil and basophil counts, thereby indicating a homogeneity in these hematological indices across genders. Conclusion: This study offers a comprehensive analysis of variations in hematological parameters across different groups, highlighting significant differences in hemoglobin , total RBC count, hematocrit, platelet count and mean platelet volume. These findings underscore the potential influence of biological and physiological factors on blood profiles, suggesting a need for personalized approaches in the interpretation of hematological data for clinical or diagnostic purposes.

Hematological parameters of the European hake (Merluccius merluccius) in Toroneos Gulf, northern Greece: A case study

Background: The European hake (Merluccius merluccius) is a commercially valuable demersal species widely distributed in the Mediterranean Sea. Assessing the condition of fish populations in their natural habitats is challenging due to the lack of reliable reference points. Objective: This study aimed to utilize hematological analysis as an economical method to evaluate the physiological and health status of European hake, addressing the gap in hematological data for this species. Methods: Blood samples were collected from the caudal vein of 40 adult European hakes caught from the Toroneos Gulf (northern Greece) using a commercial bottom otter trawl. An automated hematological analyzer was used to assess hematological parameters alongside biometric and biological indices. Results: Female hakes showed significantly higher white blood cell (WBC) counts, thrombocyte (TC) counts, and red cell distribution width (RDW) than their male counterparts. Strong correlations were observed among various hematological parameters, notably between WBC and red blood cells (RBCs), hematocrit (Ht), and hemoglobin (Hb); between RBC and both Ht and Hb; between TC and both mean platelet volume and platelet distribution width (PDW); and between mean corpuscular Hb concentration and RDW. Significant differences were noted in RBCs, Hb, and Ht compared to data from wild-caught European hake populations in Argentina and Denmark. Both trawling depth and duration were found to significantly affect RBC, WBC, Hb, and Ht values, while having no notable impact on TC. Fish captured at an average depth of 80 m and with a trawling duration of 30 min exhibited significantly elevated hematological indices. Conclusion: This study demonstrates that hematological analysis is a valuable, cost-effective tool for assessing the physiological and health status of European hake populations in the Mediterranean. Notable differences in hematological parameters based on sex, as well as significant correlations among key blood metrics, underscore the importance of understanding species-specific hematological profiles. The influence of trawling depth and duration on certain blood parameters highlights the need for standardized sampling protocols in population health assessments. These findings contribute essential baseline hematological data for European hake, facilitating more informed fisheries management and conservation strategies.

Real-world analysis of the incidence and risk factors of pneumonitis in non-small cell lung cancer patients treated with combined thoracic radiotherapy and immunotherapy

Abstract Objectives Combining radiotherapy with immune checkpoint inhibitor (ICI) treatment has emerged as an important therapeutic regimen. However, this combined treatment may increase the risk of pneumonitis. The aim of this study is to analyze the incidence and risk factors for pneumonitis in non-small cell lung cancer (NSCLC) patients receiving combined thoracic radiotherapy and ICI (RT + ICI) treatment in the real-world clinical setting, offering a reference and guidance for clinical physicians. Methods This study identified 447 patients with pathologically confirmed NSCLC at West China Hospital of Sichuan University from 2016 to 2021. Clinical characteristics, treatment regimens, immune-related adverse events (irAEs), and hematological data were collected and analyzed. Results Patients receiving combined RT + ICI treatment had a higher risk of developing pneumonitis than those receiving ICI treatment alone (26.9 vs. 6.7 %, p&lt;0.001). The multivariate logistic analysis identified the following independent risk factors for pneumonitis in patients undergoing combined RT + ICI treatment: history of lung disease (p=0.032), first-line ICI treatment (p=0.001), anti-PD-L1 instead of anti-PD-1 treatment (p=0.035), and the development of immunotherapy-related thyroid dysfunction (p=0.019). The independent risk factors were incorporated into a nomogram to predict the incidence of pneumonitis. The area under the receiver operating characteristic curve is 0.727, suggesting an acceptable predictive efficacy. Conclusions Compared to ICI monotherapy, NSCLC patients receiving the combination of thoracic radiotherapy and ICI treatment are at higher risk of developing pneumonitis. The nomogram holds promise for facilitating the risk assessment and early identification of pneumonitis in NSCLC patients receiving combined RT + ICI treatment.

CORRELATION OF OXIDATIVE STRESS MARKERS IN MULTIPLE BIOFLUIDS OF END-STAGE RENAL DISEASE PATIENTS

A study established that patients with ESRD have high oxidative stress levels that spike the progression of the disease and its complications. Knowledge about the levels of the Oxidative stress biomarkers in various biofluids including Urine, serum, and saliva can explain the oxidative condition and probable diagnostic accuracy of these markers in ESRD patients. A descriptive cross-sectional exploratory research design was used to perform a comparison of 50 ESRD patients with 50 healthy subjects. On demographic, clinical, and hematological parameters data were obtained. Blood MDA, 8-OHdG, and saliva TAC were determined as the indicators of oxidative stress. Multivariate analysis was conducted to compare the levels of these markers in the biofluids and probable neurological disorders, and the participants’ demographic and clinical data were evaluated. Demographic profiling revealed that ESRD patients’ mean BMI was 26.3 ± 3.9 kg/m², compared to controls 24.8 ± 3.4 km/m², p = 0.032; more of them being smokers’ p = 0.045. ESRD patient’s general hematologic profile showed that their mean hemoglobin level was 10.2 ± 1.5 g/dL and the mean hematocrit was 32.5 ± 4.3 % and the difference was significant when compared with the control group (p&lt;0.05). ESRD was associated with an increase in WMBC, percentage of neutrophils, and CRP level compared with the control group (p&lt;0.05). Acute phase marker study indicates that the MDA level was significantly higher in serum (5.6 ± 1.1 nmol/L) than in urine (3.2 ± 0.8 nmol/L) and saliva (2.8 ± 0.7 nmol/L, p=0.016). The levels of 8-OHdG were significantly higher in serum (10.3 ± 2.1 ng/mL) than in urine and saliva (p=0.027). Salivary TAC was the least suggesting reduced antioxidant defense (p&lt;0.05). The study revealed that ESRD patients have increased levels in the markers of oxidative stress in urine and serum samples, as well as saliva compared to healthy individuals. The studies have identified serum as the most representative biofluid for evaluating oxidative stress in ESRD and therefore assured the practical application of the results for the identification of the best candidates for early interventions.

Investigation of the Relationship Between Brown HT Dye Exposure and Mammary Tumor Development in Female Rats: An Assessment of the Potential Risk of Breast Cancer.

Azo dyes featuring one (monoazo) or several intramolecular NQN bonds are utilized in the food, pharmaceutical, and textile industries. The food azo dye chocolate brown HT (E155) adversely affects hepatic and renal function upon prolonged consumption. This study aimed to assess the carcinogenic potential of E155 in the development of mammary tumors and breast cancer. A total of 20 female Long-Evans rats (eight to nine weeks old) were randomly assigned to five groups, each consisting of four rats. The control (female control) group received a regular diet, whereas the positive control (female positive control) group received 7,12-dimethylbenz(a)anthracene. The remaining three groups received 200, 400, or 600 mg/kg body weight (BW)/day E155 for 40 weeks. Tumor development, BW, and biochemical, hematological, and histological data were monitored. BW decreased significantly with increasing dosages in the female moderate dose (FMD) group. Blood counts indicated potential microcytic anemia and inflammation in the treatment groups, especially in the female high-dose (FHD) group. E155 dose-dependently impaired renal function and increased blood creatinine and uric acid levels.Elevated serum glutamic pyruvic transaminase (SGPT) and serum glutamic-oxaloacetic transaminase levels indicate abnormal liver function. FHD animals had more tumors and larger sizes. Higher alpha-fetoprotein (AFP) and cancer antigen levels were detected even at low doses. Histopathological analysis revealed that E155 causes mammary gland fibroadenomas, ductal carcinoma in situ, and hyperplasia. It also causes circular layer granulomas, fibrosis, and crypt abscesses in the intestines of FMD and FHD. The current studysuggests that prolonged exposure to E155 may result in a higher incidence of mammary tumors, indicating an elevated risk for the onset of breast cancer.

Mosaic chromosomal alterations (mCAs) in individuals with monoclonal B-cell lymphocytosis (MBL)

MBL is a precursor condition to chronic lymphocytic leukemia (CLL), characterized by monoclonal B-cells in blood. Mosaic chromosomal alterations (mCAs) are a form of clonal hematopoiesis that include gains, losses, and copy-neutral loss-of-heterozygosity of large DNA segments. Both MBL and mCAs have been found to increase the risk of CLL and lymphoid malignancies, and the aim of our study was to investigate how mCAs relate to MBL, which is currently unknown. We analyzed genetic, flow cytometric, and hematologic data from 4632 individuals from the Mayo Clinic Biobank and CLL Database. MBL was detected using flow cytometry and classified as high-count (HC) or low-count (LC) MBL based on clone size. mCAs were detected primarily from whole blood DNA using sensitive SNP-array-based analyses. mCAs commonly altered in CLL (deletion of 6q, 11q, 13q, 17p, and trisomy 12) were specific (>99%) to individuals with MBL and CLL. HC-MBL and LC-MBL individuals were 881-fold and 8-fold, respectively, more likely to harbor CLL-associated mCAs than those without MBL. The cell fraction bearing these mCAs typically exceeded the B-cell fraction, suggesting their origin prior to the B-cell lineage. Integrating genetic and blood count data enabled detecting HC-MBL with high specificity in a biobank sample. These results quantify the contribution of mCAs to MBL and could enable large studies of HC-MBL without the need for flow cytometric screening.

Physiological effects of research handling on the northern elephant seal (Mirounga angustirostris)

Wildlife researchers must balance the need to safely capture and handle their study animals to sample tissues, collect morphological measurements, and attach dataloggers while ensuring their results are not confounded by stress artifacts caused by handling. To determine the physiological effects of research activities including chemical immobilization, transport, instrumentation with biologgers, and overnight holding on a model marine mammal species, we collected hormone, blood chemistry, hematology, and heart rate data from 19 juvenile northern elephant seals (Mirounga angustirostris) throughout a translocation experiment. Across our six sampling timepoints, cortisol and aldosterone data revealed a moderate hormonal stress response to handling accompanied by minor changes in hematocrit and blood glucose, but not ketone bodies or erythrocyte sedimentation rate. We also examined heart rate as a stress indicator and found that interval heart rate, standard deviation of heart rate, and apnea-eupnea cycles were influenced by handling. However, when seals were recaptured after several days at sea, all hormonal and hematological parameters had returned to baseline levels. Furthermore, 100 % of study animals were resighted in the wild post-translocation, with some individuals observed over four years later. Together, these findings suggest that while northern elephant seals exhibit measurable physiological stress in response to handling, they recover rapidly and show no observable long-term deleterious effects, making them a robust species for ecological and physiological research.

Standardization of transthoracic impedance values for estimating heart failure and its utility

Abstract Background The use of intrathoracic impedance for examining pleural effusion in heart failure patients has been explored previously. However, due to concerns about its accuracy and complexity, transthoracic impedance values have had limitations in quantifying extravascular lung water. Our dual objectives were to clarify the relationship between intrathoracic conditions and percutaneous transthoracic impedance values, and to establish a basic model for a new machine learning-based estimation system for assessing intrathoracic conditions in patients with heart failure. Methods First, we developed a live porcine congested lung model that induced pleural effusion by substantial fluid loading, and then longitudinally evaluated its correlation with percutaneous transthoracic impedance values. Second, we conducted multi-frequency bioelectrical impedance analysis to simultaneously collect electrical, physical, and hematological data from 63 hospitalized heart failure patients and 82 healthy volunteers Results In the porcine model, pleural effusion correlated with a concurrent decrease in SpO2 and a gradual decrease in impedance. We indexed and generated features from the measured values and developed an intrathoracic estimation model based on electrical measurements and clinical findings using a decision tree-based machine learning approach. Among the 286 features collected per individual, the model used 16 features. Notably, the model demonstrated high accuracy in discriminating patients with pleural effusion, achieving an AUC of 0.905 (95% CI: 0.870-0.940) in cross-validation, significantly outperforming the conventional frequency-based method. Conclusions Transthoracic impedance values, when indexed, reflect intrathoracic conditions and improve estimation. Our results highlighted the potential of machine learning and thoracic impedance measurement for accurate estimation of pleural effusion. This approach provides an effective means of assessing intrathoracic conditions. Figure: The upper figure shows the relationship between impedance values and pleural effusion in the congested lung model. On these results, human data were collected to construct the estimation model. The lower illustrates the accuracy of the model and provides a comprehensive view of the system.