Increase in water temperature due to anthropogenic and climatic changes is expected to affect physiological functions of fish. In this study, we determined high temperature tolerance (CTmax) of a common aquacultured Indian major carp, rohu, Labeo rohita fingerlings (15.96±0.72g BW, 11.56±0.42cmTL) followed by acclimatization at three temperatures (30, 33, 36°C). To determine the CTmax, we analyzed the major hemato-biochemical indices - hemoglobin (Hb), red blood cell (RBC), white blood cell (WBC), blood glucose levels, and erythrocytic nuclear abnormalities (ENAs) and erythrocytic cellular abnormalities (ECAs) of peripheral erythrocytes in the fish sampled at the start and end point at each acclimated temperature. Significantly decreased CTmax of the fish was found at 36°C compared to 30°C and 33°C. The fish in the highest (36°C) temperature were found with significantly lower Hb and RBC content and significantly higher WBC and blood glucose levels than that of the fishes in the lowest (30°C) temperature both at the start and end points. The highest frequencies of ENAs and ECAs were found in the highest (36°C) temperature group compared to the lowest (30°C) temperature group at both the points. We also evaluated growth performance of the rohu fingerlings reared in the three temperatures for 60 days. The growth parameters - final weight gain, percent weight gain and specific growth rate were the highest at 33°C and the lowest at 36°C. The present study revealed that the highest temperature (36°C) tested here may be hazardous to rohu and the temperature should be kept below 36°C in the aquaculture setting to avoid physiological damage and growth and production loss to the fish.