Hemato-biochemical Alterations Research Articles

Haemato-biochemical Alteration in Bacteria Associated Respiratory Tract Infection in Dog

The research was undertaken during October, 2022 to June, 2023 to study the changes in haemato-biochemical values of the dogs suffered from respiratory tract infection due to bacteria. Blood samples were collected from healthy control group (Group A) and each dog of different treatment groups (Group B, Group C and Group D) on the 0th day (pre-treatment) and the 10th day (post-treatment) in K3-EDTA vacutainer tubes as well as clot activator-coated vacutainer tubes for haemato-biochemical estimation. In the result of haemato-biochemical study the highly significant difference (**p<0.01) was found in total leucocyte count, absolute neutrophil count and C-reactive protein. Statistically no significant difference (p>0.05) was observed in total erythrocyte count, haemoglobin, absolute eosinophil count, absolute lymphocyte count and albumin. In case of total protein a highly significant difference (**p<0.01) was observed between Group A and Group B on day 0 and on day 10. A highly significant difference was also observed between (**p<0.01) Group B and Group C; Group B and Group D on day 0 and day 10. On the other hand no significant difference (p>0.05) was observed within treatment groups. In case of globulin, statistically no significant difference (p>0.05) was obtained between the Group A and all the treatment groups on day 0 and day 10. No significant difference (p>0.05) was observed within treatment groups. However, a significant difference (**p<0.01) was found between Group B and Group C; Group B and Group D on 0th day and on the 10th day.

Biomorphometric and hematobiochemical alterations in the juvenile african catfish Clarias gariepinus exposed to propranolol

Propranolol (PRO) is a beta-blocker drug used for the treatment of anxiety, chest pain, migraine and tremors. The present study investigated whether sublethal concentrations of PRO have effects on the body condition, biochemistry, and hematology of Clarias gariepinus juveniles. The 96-h median lethal concentration (LC50) of the drug, which was established through an acute toxicity study, was 9.48 mg/L. Based on these values, the fish were exposed for 21 days to the control and sublethal concentrations of 1.90, 0.95, and 0.63 mg/L, which are equivalent to the 1/5th, 1/10th, and 1/20th of the LC50 of PRO, respectively. After 21 days of exposure, the fish were removed from the toxicant and kept in toxicant-free water for 7 days to recover. The standard length and body weight of each fish were measured after each exposure period. The condition factor (CF) and hepatosomatic index (HSI) were not significantly affected by the drug. The red blood cell (RBC) count, hemoglobin (Hb) count and packed cell volume (PCV) decreased from day 7 to 21 at the tested concentrations, while the white blood cell (WBC) count significantly increased. There were alterations in the mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and mean corpuscular hemoglobin (MCH) in the exposed groups compared to those in the control group. When neutrophil counts increased, the lymphocyte counts decreased, but the monocyte, basophil cell and eosinophil cell counts were not affected. Among the liver enzymes, only aspartate aminotransferase was significantly stimulated in the groups that were exposed to the drug. The protein and glucose levels of fish exposed to the drug decreased. Most of the studied parameters returned to their original values after the 7-day recovery period. The information provided in the current study will be helpful in the monitoring of PRO contamination in aquatic environments.

Some haematobiochemical alterations in buffaloes suffering from milk fever with trail of treatment

Some haematobiochemical alterations in buffaloes suffering from milk fever with trail of treatment

Clinical abnormalities and haemato biochemical alterations in dogs affected with parvovirus infection

Clinical abnormalities and haemato biochemical alterations in dogs affected with parvovirus infection

Haematobiochemical alterations in cattle infected with Bovine tropical theileriosis in and around Tirupati district

Haematobiochemical alterations in cattle infected with Bovine tropical theileriosis in and around Tirupati district

Study on Hemato-biochemical Alterations, Oxidative Stress and Epidemiology of Parasitic Infestation along with Therapeutic Effect of Eprinomectin in Sheep in South Punjab

Study on Hemato-biochemical Alterations, Oxidative Stress and Epidemiology of Parasitic Infestation along with Therapeutic Effect of Eprinomectin in Sheep in South Punjab

Haematobiochemical alterations in equines with various pulmonary diseases

Haematobiochemical alterations in equines with various pulmonary diseases

Fipronil Toxicity Induced Hemato-Biochemical Alterations in Male Wistar Albino Rats and its Alleviation with Pomegranate Peel Extract (Punica granatum)

Fipronil Toxicity Induced Hemato-Biochemical Alterations in Male Wistar Albino Rats and its Alleviation with Pomegranate Peel Extract (Punica granatum)

A study on assessing the toxic effects of ethyl paraben on rohu (Labeo rohita) using different biomarkers; hemato-biochemical assays, histology, oxidant and antioxidant activity and genotoxicity.

Parabens are being used as preservatives due to their antifungal and antimicrobial effects. They are emerging as aquatic pollutants due to their excessive use in many products. The purpose of this study was to determine the toxic effect of ethyl paraben (C9H10O3) on the hematobiochemical, histological, oxidative, and anti-oxidant enzymatic and non-enzymatic activity; the study also evaluates the potential of ethyl paraben to cause genotoxicity in Rohu Labeo rohita. A number of 15 fish with an average weight of 35.45±1.34g were placed in each group and exposed to ethyl paraben for 21 days. Three different concentrations of ethyl paraben, i.e., T1 (2000μg/L), T2 (4000 μg/L), andT3 (6000 μg/L) on which fish were exposed as compared to the control T0 (0.00 μg/L). Blood was used for hematobiochemical and comet assay. Gills, kidneys, and liver were removed for histological alterations. The results showed a significant rise in all hemato-biochemical parameters such as RBCs, WBCs, PLT count, blood sugar, albumin, globulin, and cholesterol. An increase in aspartate aminotransferase (AST) and alanine transaminase (ALT) levels directed the hepatocytic damage. Histological alterations in the liver, gills and kidneys of fish were found. Ethylparaben induces oxidative stress by suppressing antioxidant enzyme activity such as SOD, GSH, CAT and POD. Based on the comet assay, DNA damage was also observed in blood cells, resulting in genotoxicity. Findings from the present study indicate that ethyl paraben induces hemato-biochemical alterations, tissue damage, oxidative stress, and genotoxicity.

Ameliorative effect of Anthocephalus cadamba, Brassica juncea and Pithecellobium dulce against fipronil-induced hemato-biochemical alterations in rats

Ameliorative effect of Anthocephalus cadamba, Brassica juncea and Pithecellobium dulce against fipronil-induced hemato-biochemical alterations in rats

Effects of Quercetin on Aspirin-Induced Haemato-Biochemical Alterations in Wistar Rats

Aspirin is a non-steroidal anti-inflammatory drug (NSAID). Quercetin is natural flavonoid present in high concentration in herbal drugs, fruits and vegetables and demonstrating broad bio-pharmacological properties. This study was aimed to evaluate the ameliorative effects of quercetin (@ 100 & 200 mg/kg b.wt.) in aspirin (@ 100 mg/kg b.wt.) induced haemato- biochemical alterations in Wistar rats (n=36) over a period of 28 days. There was significant decrease in mean values of haemoglobin, packed cell volume, platelets and increase in total leucocyte count in aspirin treated rats (Gr. II) over the control group (Gr I) at 28th day of experiment. Similarly, in biochemical parameters also there was significant elevation in aspartate aminotransferase, alanine aminotransferase, urea and creatinine and significant decrease inmean values of total protein, albumin, triglyceride and cholesterol in aspirin treated rats. Administration of quercetin at higher dose rate of 200 mg/kg b.wt. for 28 days (Gr. III) along with the aspirin in Wistar rats prevented such alterations in different haemato-biochemical parameters as the values on day 28 were almost similar to those on day 0 and at par with control group. Hence, quercetin @ 200 mg/kg b.wt. along with aspirin @ 100 mg/kg b.wt. can be recommended to avoid aspirin induced health issues in high risk mammals.

Molecular Diagnosis and Haemato-biochemical Alteration in Canine Parvoviral Infection in Dogs

The present study was undertaken to diagnose and assess the haematological & biochemical alteration in parvoviral gastroenteritis in dogs. Total of 12 dogs suspected for parvovirus infection were diagnosed by using lateral flow-based immunochromatographic assay rapid canine parvovirus (CPV) antigen test kit and polymerase chain reaction (PCR) technique in the faeces of affected dogs. All 12 dogs were found positive by a rapid antigen test kit and VP2 gene of CPV PCR assay using the CPV-2ab primer pair and expected size of 681 bp. The haemato-biochemical study revealed a decrease in Hb, TEC, serum total protein, albumin and globulin, whereas the increase in absolute neutrophil count and BUN in parvovirus-infected dogs, indicated anemia and hypoproteinemia in parvovirus infected dogs.

Clinical findings, haemato-biochemical alterations and conservative treatment of caecal dilatation in a buffalo

This article describes the clinical findings, haemato-biochemical alterations, and conservative treatment of a buffalo suffering from caecal dilatation. The clinical findings were anorexia, scanty faces, reduced rumen motility, distension of right flank, ping sounds on auscultation and percussion of right flank and dilated caecum at pelvic brim on per-rectal examination. The hemato-biochemical alterations were neutrophilic leukocytosis, increased AST, BUN and lactate, and low calcium levels. Conservative treatment resulted in uneventfully after a period of five days.

Haemato-biochemical alterations associated with the use of eggshell membrane as a dressing material for full-thickness wounds in a rabbit model

Haemato-biochemical alterations associated with the use of eggshell membrane as a dressing material for full-thickness wounds in a rabbit model

Clinico-therapeutic Studies on Haemonchosis in Goats

Background: Haemonchosis is one of the major parasitic infestations in small ruminants. Haemonchus contortus is a highly pathogenic parasite that localises in the abomasum of affected animals and exerts its pathogenicity by blood-sucking activity, adversely affecting the health and productivity of animals. In recent years, there is widespread development of anthelmintic resistance against Haemonchus spp. Therefore, a sustainable strategy needs to be implemented to effectively control haemonchosis in goats. The aim of the present study was to find out the efficacy of herbal anthelmintic drug (Carica papaya aqueous seed extract) and haemato-biochemical alterations in Haemonchus infection. Methods: In this study, goats were screened for Haemonchus spp. by the FAMACHA technique. Hemato-biochemical examination was done before and after the therapeutic trial. Herbal anthelmintic drug was prepared from Papaya seed. In-vitro and in-vivo trial were carried out to test the efficacy of prepared herbal anthelmintic drug. Result: The present study showed that FAMACHA can be used for the early screening of Haemonchus spp. in a herd. Significant improvement in haemato-biochemical examination was found in the group treated with the prepared herbal anthelmintic drug. Thus, the herbal anthelmintic (Carica papaya seed extract) can be a good alternative for controlling Haemonchosis in goats.

Haemato-biochemical Alterations in Canine Rotaviral Enteritis of Dogs and its Management

Background: Rotaviruses cause acute diarrhoea, mostly in young puppies. Rotavirus associated gastroenteritis in dog is of great concern not only because of their high morbidity and mortality, but became of the reason that, they act as potential source of zoonotic infection and inter-species transmission. There is no report on rotavirus infection in dog from Assam. Therefore, there is a need to study the haemato-biochemical alterations in rotavirus infected dogs while treating such type of clinical cases. Methods: A total of 157 faecal and blood samples were collected from the dogs affected with gastroenteritis, registered at Veterinary Clinical Complex, Khanapara, Assam during November, 2020 to April, 2021. Diagnosis of rotavirus was done by RNA-PAGE and RT-PCR. Haematological study of the canine rotavirus (CRV) infected dogs were carried out with automated haematological cell counter and biochemical analysis was done with semi-automated biochemistry analyser using commercial kit. Result: Out of 157 samples, 8 samples were found positive for canine rotavirus by RNA-PAGE and 17 samples by RT-PCR. Haemato-biochemical alterations in canine rotavirus infected dogs revealed significant increase in Hb, PCV, TEC, monocyte, lymphocyte, AST, ALT, BUN and creatinine while significant decrease in TLC, neutrophil, total protein, serum sodium, potassium and chloride.

Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different physiological responses of male African catfish, Clarias gariepinus, and the ameliorative effect of Chlorella vulgaris. The fish (160 ± 5.10 g) were exposed to 1/20 LC50 of OXY (0.58 mg/L) for 60 consecutive days with or without co-administration of C. vulgaris (25 g/kg diet) in triplicate groups. The results revealed that chronic exposure to a sublethal level of OXY induced severe anemia and leukopenia. OXY-exposed fish experienced hypoproteinemia, marked lower AchE levels, and a significant increase in glucose, liver, and kidney function biomarkers. The DNA fragmentation of the liver increased by 15 % in fish compared to the control. On the other hand, lipid peroxidation, superoxide dismutase, and catalase activities were markedly increased in the liver and testes homogenates of the OXY-exposed fish. Meanwhile, total antioxidant capacity and glutathione S-transferase levels declined in the same tissues. Exposure to OXY induced a significant reduction in testosterone and luteinizing hormone levels and a significant increase in follicle stimulating hormone and estradiol. Meanwhile, C. vulgaris dietary supplementation succeeded in alleviating the negative impact of OXY on hematobiochemical parameters and restoring the antioxidant balance in the liver and testes. Furthermore, it ameliorated endocrine disruption and repaired sex hormone levels. In conclusion, exposure to OXY could induce systemic stress, oxidative stress, and endocrine disruption in male C. gariepinus. The dietary supplementation of C. vulgaris could be a potential protective strategy against the toxicity of OXY.

Insights into the Prognostic Role of Serum Interleukin-6 and Hematobiochemical Alterations in Cattle during Recent Outbreaks of Lumpy Skin Disease in Lodhran District, Pakistan.

Lumpy skin disease (LSD) is a highly infectious disease of cattle caused by a virus of the Poxviridae family, genus Capripoxvirus. The present study was designed to determine the prognostic ability of serum IL-6 in LSD using a binary logistic regression model at baseline sampling. A 17-day cohort study was conducted on a recent outbreak of LSD among cattle in the Lodhran District of Punjab, Pakistan. Infected cattle were divided into two categories based on their clinical status on day 17 as recovered (n = 33) or unrecovered (n = 17). Nodular lesions and scab specimens (n = 50) were used for the isolation of the lumpy skin disease virus and were confirmed by PCR. In recovered animals, hematological results showed marked leukocytosis, eosinophilia, lymphocytosis, neutrophilia, and monocytopenia. However, marked erythrocytosis, leukopenia, and thrombocytopenia were observed in the unrecovered animals at the final sampling point of the study. Serum levels of total protein, albumin, and glucose were significantly higher in the recovered animals. Meanwhile, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine phosphokinase, total bilirubin, and direct bilirubin were found considerably higher in the unrecovered group. Receiver-operating characteristic curve analysis for serum IL-6 at baseline predicts the extended clinical conditions at the cut-off value of 85.16 pg/mL (55% specificity, 94% sensitivity, area under the curve 0.8039, respectively). In conclusion, the disease-induced hematological and biochemical alterations were significantly ameliorated in the recovered animals. In addition, the study revealed that serum IL-6 can be used as a valid marker for predicting the clinical worsening of LSD in cattle.

CLINICAL AND HEMATO-BIOCHEMICAL STUDIES ON GOATS NATURALLY INFESTED WITH SUCKING AND BITING LICE

The current study was performed to assess the most common clinical symptoms and hemato-biochemical alterations in goats naturally infested with sucking and biting lice. A total of 4599 goats were included in the clinical investigation. The changes in concentrations of some hematological and biochemical parameters were evaluated using 20 naturally infested animals divided into 2 equal groups – group 1 (infested with Bovicola caprae) and group 2 (infested with Linognathus stenopsis). Blood samples were collected before the treatment with Eprinomectin and on the 30th and 60th-day post-treatment (DPT). After sampling, the blood was examined for changes in the complete blood cell count including RBC, HGB, HCT, RBC indices (MCV, MCH, MCHC, RDW), WBC, leukocyte differential count as well as some biochemical parameters (Ca, P, Fe, total protein, albumin, bilirubin, ASAT, ALAT, AP). Clinical demonstrations in infested goats were not specific, represented with rubbing, scratching, broken hairs, alopecia, skin secretion, inflamed lesions (papules), crusts, and keratinization. The changes found in the hematological and biochemical parameters were normocytic hypochromic anemia (p˂0.001) and hypoferremia (p˂0.01) in the goats infested with sucking lice (L. stenopsis) and leukocytosis (p˂0.001), hyperproteinemia (p˂0.001) and hyperalbumineamia (p˂0.05) in the animals infested with biting lice (B. caprae). All changed parameters have returned to the reference ranges on the 30th and 60th DPT.

Haemato-biochemical alterations in naturally occurring babesiosis in dogs

Haemato-biochemical alterations in naturally occurring babesiosis in dogs