Helicobacter Pylori Specific IgG Research Articles

Relationship of Helicobacter Pylori Specific IgG Antibodies with Serum Magenisum and Serum Lipid Profile in Patients on Maintenance Hemodialysis

Background: Helicobacter pylori are thought to play an important role in the pathogenesis of activegastritis and other upper mucosal lesions in chronic hemodialysis patient which thoughtto result in dyspeptic symptoms. Aim:The aim of the study is to determine the prevalence of Helicobacter pylori infection in patient on maintenance hemodialysis and to assess the probable association of serum lipid profile and serum magnesium with H. pylori infection in these patients. Material &Methods: This cross-sectional study was conducted on patients with end stage renal disease who were undergoing maintenance hemodialysis treatment.were enrolled in the present studya total number of 42 hemodialysis patients (24 male and 18 female), of the total number; 10 were diabetic and 32 were non diabetic. Demographic data including age, gender, duration of hemodialysis and clinical data including diabetes mellitus, upper gastrointestinal symptoms and laboratory measurements were obtained from related questionnaires. Venous blood samples were collected for biochemical analysis of the following markers: serum magnesium, total cholesterol, triglyceride; high density lipoprotein-cholesterol, low density lipoprotein-cholesterol and very low density lipoprotein-cholesterol. Result & Conclusion: We conclude that there was no significant correlation between gender, age, duration of hemodialysis, DM, serum triglyceride, VLDL-C and magnesium and H. pylori infection in these patients.

Comparison of enzyme immunoassays detecting Helicobacter pylori specific IgG in serum and saliva with endoscopic and biopsy findings in patients with dyspepsia

Purpose: To compare the performance of two indirect enzyme-linked immunosorbent assays (ELISA) detecting Helicobacter pylori (HP)-specific IgG antibodies in serum and saliva with endoscopic observations and histologic findings of biopsies from dyspeptic patients, in an area of high HP prevalence. Materials and Methods: Sera, saliva and antral biopsies were obtained from 55 dyspeptic patients. IgG antibodies against HP were assayed in sera and saliva utilizing two indirect ELISAs. Biopsies were processed according to standard procedures in order to detect histological changes and the presence or absence of Helicobacter pylori. Laboratory data thus obtained were compared and statistically analyzed. Results: Forty-two (76.36%) biopsies were positive for HP. The organisms were detected in 4 of 16 (25%) cases with normal endoscopic findings, in all 16 cases of gastritis and in 22 of the 23 (95.6%) cases of duodenal ulcers (DU). Serum and saliva HP-specific IgG antibodies were detected in 4 normal cases with positive biopsies, in 12 and 14 cases of gastritis, respectively, and in all 22 (100%) biopsy positive cases of DU. The sensitivities of the serum and saliva tests were 90.5% and 95%, respectively, while the specificities were 84.5% and 70%, respectively. Conclusion: Due to their high sensitivity and specificity in diagnosing HP-associated DU and gastritis, serum and saliva antibody testing seems to offer a valuable alternative to invasive procedures especially in areas of high HP prevalence such as ours; saliva antibody testing is simple and practical especially in children and in difficult patients who resent venipuncture.

Helicobacter pylori infection is associated with decreased serum levels of high density lipoprotein, but not with the severity of coronary atherosclerosis

ObjectiveThe objective of this survey was to study the association between Helicobacter pylori infection and the severity of coronary atherosclerosis.MethodsThe study population consisted of 961 consecutive patients (711 males and 250 females) who underwent coronary angiography for suspected or known coronary atherosclerosis. The patients' body mass index, blood pressure, the blood lipid, blood glucose, leukocyte count (109/L), neutrophil count (109/L), and Helicobacter pylori-specific IgG antibodies were performed. Coronary angiograms were scored according to vessel score and Gensini's score.ResultsA significant association between H. pylori infection and coronary atherosclerosis as well as its severity was not find in this cross section study (p = 0.858). And, the level distribution of vessel score (p = 0.906) and Gensini's score (p = 0.905) were similar in the seropositivity group and seronegativity group of Helicobacter pylori infection. However, the level of fasting high-density lipoprotein cholesterol (mmol/L) (p = 0.013) was significantly lower in the seropositivity group than that in the seronegativity group of Helicobacter pylori infection.ConclusionsIn conclusion, in the present study, a significantly correlation between Helicobacter pylori seropositivity and angiographically evaluated severity of atherosclerosis was not find. And, the present study showed a good correlation between Helicobacter pylori infection and decreased HDL cholesterol. However, the exact mechanisms need further study.

Correlation of Serum Leptin with Circulating Anti-Helico Bacter Pylori IgG Antibodies in End-Stage Renal Failure Patients on Regular Hemodialysis

Leptin is synthesized mainly by the adipose tissue. Leptin also is detected in gastric chief cells. To consider the association of serum leptin with helicobacter pylori- IgG antibodies as parameters of nutritional status, on a group of hemodialysis patients, serum Leptin and serum helicobacter pylori specific IgG (H. Pylori-IgG) antibody titer were measured. Total patients were 36. In all patients there were a significant positive correlation of serum leptin with BMI and a near significant positive correlation of serum leptin with age. In the diabetic HD group a significant inverse correlation of helicobacter pylori IgG antibody titer and serum leptin was seen. This is the first report of the association of serum leptin with pylori-IgG antibodies in hemodialysis patients which may explain nutritional problems that is frequently seen in diabetic hemodialysis patients .More research is needed regarding the clinical consequences of this association.

Preferential placental transfer of Helicobacter pylori specific IgG

Objective: To evaluate the placental transfer of Helicobacter pylori-specific IgG in Iranian mothers. Method: The antibodies were measured in sera of 156 mother/newborn pairs using a commercially available indirect Enzyme Linked Immunosorbent Assay (ELISA). The study population was among healthy pregnant women who attended to the Zeinabieh hospital of Shiraz University of Medical Sciences in 1999. Results: In total 74.7% of mothers were seropositive and more than 82% of seropositive mothers transferred Helicobacter pylori-specific IgG antibodies to their fetuses. The mean maternal Helicobacter pylori-specific IgG was significantly higher than that of the newborns (104.01 vs. 68.30 IU/ml, p < 0.001), however, there was a good correlation between maternal and neonatal antibodies. The level of maternal Helicobacter pylori-specific IgG was significantly lower in carriers of blood group B + compared to carriers of blood groups A + and O + . However, the cord/maternal ratio of Helicobacter pylori-specific IgG was significantly higher in blood group B + phenotype compared to blood group phenotypes A + and O + . Conclusion: The high rate of seropositivity among mothers highlights the risk of acquisition of Helicobacter pylori infection in early infancy for Iranian children. Our results also suggest that mothers having blood group B + are more likely to transfer Helicobacter pylori-specific IgG to their neonates.

Preferential placental transfer of Helicobacter pylori specific IgG

To evaluate the placental transfer of Helicobacter pylori-specific IgG in Iranian mothers. The antibodies were measured in sera of 156 mother/newborn pairs using a commercially available indirect Enzyme Linked Immunosorbent Assay (ELISA). The study population was among healthy pregnant women who attended to the Zeinabieh hospital of Shiraz University of Medical Sciences in 1999. In total 74.7% of mothers were seropositive and more than 82% of seropositive mothers transferred Helicobacter pylori-specific IgG antibodies to their fetuses. The mean maternal Helicobacter pylori-specific IgG was significantly higher than that of the newborns (104.01 vs. 68.30 IU/ml, p < 0.001), however, there was a good correlation between maternal and neonatal antibodies. The level of maternal Helicobacter pylori-specific IgG was significantly lower in carriers of blood group B + compared to carriers of blood groups A + and O + . However, the cord/maternal ratio of Helicobacter pylori-specific IgG was significantly higher in blood group B + phenotype compared to blood group phenotypes A + and O + . The high rate of seropositivity among mothers highlights the risk of acquisition of Helicobacter pylori infection in early infancy for Iranian children. Our results also suggest that mothers having blood group B + are more likely to transfer Helicobacter pylori-specific IgG to their neonates.

Evidence for Helicobacter pylori specific IgG secretion by the human gastric mucosa

Evidence for Helicobacter pylori specific IgG secretion by the human gastric mucosa

IgG and IgA Antibodies in Helicobacter pylori Infections

We compared Helicobacter pylori-specific IgG and IgA antibodies in serum, salivary and gastric juice of H. pylori-infected and noninfected patients using the ELISA method. Serum and salivary anti-H. pylori IgG and IgA titres were higher in H. pylori-positive than H. pylori-negative patients. Serum H. pylori IgG titres were higher than those of serum-specific IgA. In contrast, H. pylori IgA titres in saliva samples were higher than those of specific IgG. In all patients, the levels of IgG antibodies were very low in gastric juice. H. pylori-positive patients with increased levels of IgA in sera, also exhibited increased IgA levels in gastric juice. The determination of IgA antibodies in serum and saliva may be used as a test that complements the IgG antibody assay.

Relationship between local immune response to Helicobacter pylori and the diversity of disease: investigation of H. pylori-specific IgA in gastric juice.

In order to evaluate the relationship between local immune response to Helicobacter pylori and the diversity of disease, 77 asymptomatic subjects who underwent a health examination were studied. Helicobacter pylori-specific IgG in serum and H. pylori-specific IgA in gastric juice were measured by ELISA, and the measured IgA titre was classified into two grades, low or high. Histological classification of gastritis was performed according to the Sydney system. Cytokines in gastric juice were also measured, and the cytotoxin-associated gene A (cagA) status of H. pylori was tested by PCR. Of the 65 subjects who were positive for H. pylori-specific IgG in serum, 38 (58.5%) were classified as H. pylori-specific IgA low titre in gastric juice and 27 (41.5%) had high titres. In the IgG-positive, IgA-low group, the rate of peptic ulcers (especially duodenal ulcers) in endoscopic findings was higher (P < 0.05); the score of activity and the density of H. pylori were higher (P < 0.001 and P < 0.05, respectively); the score of metaplasia was lower (P < 0.05); and the level of interleukin-1 beta was lower (P < 0.05) than in the IgG-positive, IgA-high group. The positive rate of the cagA gene was 84.4% and there was no significant difference between the two groups. There were differences in endoscopic and histological findings between the IgG-positive, IgA-low and the IgG-positive, IgA-high groups. It is suggested that persons infected with H. pylori can be divided into two different states of disease according to local immune response.

Helicobacter pylori-specific IgG in chronic haemodialysis patients: relationship of hypergastrinaemia to positive serology

Helicobacter pylori-specific IgG in chronic haemodialysis patients: relationship of hypergastrinaemia to positive serology

Helicobacter pylori-specific IgG in chronic haemodialysis patients: relationship of hypergastrinaemia to positive serology

Chronic haemodialysis (HD) patients frequently suffer from dyspeptic symptoms and hypergastrinaemia is a common finding in these patients. Helicobacter pylori (H. pylori) infection is associated with dyspepsia and hypergastrinaemia. The aim of this study was to determine whether H. pylori is frequently found in HD patients and to explore the relationship of H. pylori with dyspeptic symptoms and/or hypergastrinaemia in these patients. Serum H. pylori specific IgG were measured by an in-house enzyme-linked immunosorbent assay (sensitivity and specificity is 97% and 91% respectively) in 103 chronic HD patients. The patients (53 M, 50 F, mean age f60 +/- 13 years) completed a questionnaire exploring the type, frequency and intensity of dyspeptic symptoms. Fasting plasma gastrin levels were also measured. Serum and plasma samples from 103 hospital patients matched for age, sex and dyspepsia were use as controls. There was no significant difference in terms of serum H. pylori IgG between HD patients and controls (0.977 +/- 0.295 vs 1.046 +/- 0.306 OD respectively). The prevalence of subjects with positive serology was relatively high in both groups, but did not differ between HD patients (73%) and controls (78%). Dyspepsia was reported in 72 (70%) cases. There was no relationship between presence (and grading) of dyspepsia or type of dyspeptic symptoms and H. pylori serology. In the HD group, patients seropositive for H pylori had a significantly higher gastrinaemia than those who were seronegative: 598 +/- 413 ng/ml vs 309 +/- 252 ng/ml (P < 0.0001). The relationship between seropositivity for H. pylori and hypergastrinaemia was significant (P = 0.00038), after adjustment by multiple regression analysis for sex, age, smoking, alcohol, months on dialysis, renal function, drugs, and dyspepsia. Data of this study suggest that H. pylori may play a role in contributing to hypergastrinaemia of HD patients.