Helicobacter Pylori IgG Research Articles

Vitamin D3 is well correlated with anti-Helicobacter pylori immunoglobulins and could be a well biomarker for immunity competence against the disease

Helicobacter pylori (HPY) provokes gastrointestinal disorders and gastric cancer. We supposed that HPY disrupts the 25-OH-Vitamin-D3 (Vit.D3) absorption. We evaluated the association between Vit.D3 and anti-HPY immunoglobulins (Igs) and the Vit.D3 potency as a predictive biomarker for HPY infection. 603 patients' raw data were gathered from a private clinical laboratory. Anti-HPY Igs including serum IgG, IgA, and IgM, in addition to HPY-stool antigen, were assessed by the immunoassay methods. Vit.D3 was determined by high-pressure liquid chromatography. Correlations, ordinal comparisons, cutoff points (COP), and odds ratio (OR) were estimated. The age mean ± standard deviation was 39.83 ± 18.426 for female and 38.82 ± 16.937 for male participants (P = 0.521). Significant correlations existed after age and gender adjustment between Vit.D3 serum levels and the HPY IgG (R = 0.298) and IgA (R = 0.271) but not for IgM (R = -0.103). Approximately, 48% of males and 36% of females had insufficient/deficient Vit.D3 serum levels (male/female OR: 1.65; 1.16-2.33; P = 0.0051). After age and gender adjustment, the best COP of Vit.D3 to predict an HPY IgG-positive patient was Vit.D3 >32.80 ng/mL with 66.23% diagnostic accuracy (DAAC), 30.43% specificity (SPC), and 90.41% sensitivity (SEN). For the HPY IgA, the values were Vit.D3 >37.83 ng/mL, DAAC = 60.45%, SPC = 58.82%, SEN = 64.20%. For HPY IgM, the values were Vit.D3 >37.32 ng/mL, DAAC = 58.97%, SPC = 57.33%, and SEN = 100%. Vit.D3 had a good association with anti-HPY Igs and may be a good biomarker for immunity competence against HPY infection if the patient's age and gender are considered when interpreting the laboratory results.

Assessing the level of infectious and inflammatory factors in acute ischemic stroke in diabetic and non-diabetic patients

Introduction. Stroke is one of the principal leading causes of death globally. In 2005, stroke cause approximate 5.7 million death, 87% of deaths was in low and middle-income countries. Recently period, collecting evidence indicated that inflammation and atherosclerosis play important roles in stroke evolution. Material and method. In this prospective longitudinal observational study including 340 patients with acute ischemic stroke with / without diabetes mellitus we analyzed as main criteria infectious factors, inflammatory factors and biochemical factors. The patients were divided into 2 groups: 101 diabetic patients (study group) and 239 non-diabetic patients (control group), were we analyzed as main criteria infectious factors (cytomegalovirus IgG plasma level, cytomegalovirus IgM plasma level, Helicobacter pylori IgG plasma level), inflammatory factors (leucocyte, C-reactive protein) and biochemical (plasma level of fibrinogen). Results. Cytomegalovirus-IgG levels are lower in diabetic versus non-diabetic patients with an arithmetic mean of 1170 AU/ml (95% CI 862.4169 to 1477.8326) versus 1398 AU/ml (95% CI 1169.6839 to 1627,2042) but this difference, although it exists, is not statistically significant p = 0.123. The cytomegalovirus-IgM level is lower in diabetic versus non-diabetic patients a statistically significant difference, p 0.001. The Helicobacter IgG level is lower in diabetic patients with a mean value of 1.0763 U/ml with a 95% CI from 0.8141 to 1.3386 versus non-diabetics with an average of 1.3943 U/ml with a CI of 95% from 1.1963 to 1.5923, but this difference, although there, is not statistically significant, p = 0.07.The number of leukocytes diagnosed in diabetic patients is lower than that of people without diabetes (p = 0.0001). The level of C-reactive protein at diagnosis of diabetic patients is lower (an average value of 3.0207mg/dl with a 95% CI of 0.9726 to 5.0688) than in people who do not suffer from diabetes (arithmetic mean of 5.8218 mg/dl with a 95% CI of 0.5894 to 11.0542), but this difference is not statistically significant. The serum level of fibrinogen at the diagnosis of diabetic patients is lower, with a mean value of 399.4 mg/dl with a 95% CI of 327.7993 to 471.0749, than that of people without diabetes, with an arithmetic mean of 653.8 mg/dl, with a 95% CI of 497.8700 to 809.8647, the difference being statistically significant, p = 0.041. Conclusions. Inflammatory markers such as leukocyte levels at intake, C-reactive protein (CRP), and fibrinogen levels showed lower levels at admission in diabetic patients versus non-diabetic patients. There is no statistically significant difference between diabetic vs. non-diabetic patients regarding cytomegalovirus IgG levels, referring to cytomegalovirus IgM level is lower in diabetic versus non-diabetic patients with significant statistically difference. The Helicobacter IgG level is lower in diabetic patients versus non-diabetics patients.

Detection of Helicobacter pylori Infection by Helicobacter pylori IgG Serology Test in Pediatric Patients at the Philippine General Hospital

Detection of Helicobacter pylori Infection by Helicobacter pylori IgG Serology Test in Pediatric Patients at the Philippine General Hospital

Deep learning analyzes Helicobacter pylori infection by upper gastrointestinal endoscopy images.

Background and study aims Helicobacter pylori (HP)-associated chronic gastritis can cause mucosal atrophy and intestinal metaplasia, both of which increase the risk of gastric cancer. The accurate diagnosis of HP infection during routine medical checks is important. We aimed to develop a convolutional neural network (CNN), which is a machine-learning algorithm similar to deep learning, capable of recognizing specific features of gastric endoscopy images. The goal behind developing such a system was to detect HP infection early, thus preventing gastric cancer. Patients and methods For the development of the CNN, we used 179 upper gastrointestinal endoscopy images obtained from 139 patients (65 were HP-positive: ≥ 10 U/mL and 74 were HP-negative: < 3 U/mL on HP IgG antibody assessment). Of the 179 images, 149 were used as training images, and the remaining 30 (15 from HP-negative patients and 15 from HP-positive patients) were set aside to be used as test images. The 149 training images were subjected to data augmentation, which yielded 596 images. We used the CNN to create a learning tool that would recognize HP infection and assessed the decision accuracy of the CNN with the 30 test images by calculating the sensitivity, specificity, and area under the receiver operating characteristic (ROC) curve (AUC). Results The sensitivity and specificity of the CNN for the detection of HP infection were 86.7 % and 86.7 %, respectively, and the AUC was 0.956. Conclusions CNN-aided diagnosis of HP infection seems feasible and is expected to facilitate and improve diagnosis during health check-ups.

Relationship between Nausea, Vomiting and Helicobacter pylori IgG Seropositivity in Pregnants

Relationship between Nausea, Vomiting and Helicobacter pylori IgG Seropositivity in Pregnants

Detection and analysis on serum antibodies for five common pathogenic microbes in patients with Posner-Schlossmarm syndrome

Background Posner-Schlossman syndrome (PSS) is often recurrent and is a cause of blindness.The etiology of PSS remains to be elucidated.It is reported that there is a certain association between pathogenic microorganisms and PSS in rather small samples. Objective This study was to analyze the related serum antibody levels of cytomegalovirus (CMV), herpes simplex virus (HSV), rubella virus (RV), helicobacter pylori (HP) and anti-streptolysin O (ASO) and provide a clue for the study on pathogenesis and therapy of PSS. Methods A prospective cases-controlled study was carried out in Shenzhen Eye Hospital from December, 2014 to December, 2016 under the approval of Ethic Committee of this hospital and informed consent of each subject prior to initial of any medical examination.Peripheral blood samples were collected from 82 PSS patients as the PSS group and 100 age-and gender-matched healthy blood donors as the normal control group.The positive rates of serum CMV IgG, CMV IgM, HSV IgG, HSV IgM, RV IgG, RV IgM, HP IgG and HP IgM in the subjects were detected by indirect ELISA, and the positive rate of serum ASO antibody was determined by immuno-scatter turbidmetry. Results The positive rates of serum CMV-IgG, CMV-IgM, HP-IgG, HP-IgM and ASO antibody were 22.0%, 17.1%, 22.0%, 17.1% and 17.1% in the PSS group, which were significantly higher than 5.0%, 0.0%, 10.0%, 2.0% and 7.0% in the normal control group (χ2=11.726, 18.496, 4.943, 12.766, 4.479, all at P 0.05). Conclusions CMV, HP and hemolytic streptococcal infection may participate in the occurrence and development of PSS. Key words: Posner-Schlossman syndrome; Etiology; Cytomegalovirus; Herpes simplex virus; Rubella virus; Helicobacter pylori; Anti-streptolysin O; Antibody

Point-of-care Helicobacter pylori testing: primary care technology update.

#### Clinical Question In adults attending primary care with upper gastrointestinal symptoms, what is the accuracy and utility of point-of-care testing to detect Helicobacter pylori infection? Helicobacter pylori (HP) infection causes approximately 5% of uninvestigated dyspepsia and a 20% lifetime risk of peptic ulcer disease.1 HP is a grade 1 carcinogen: 5.2% of cancers globally are attributable to HP infection.2 HP eradication results in: reduced gastric cancer incidence; prevention of recurrent duodenal (number needed to treat [NNT] = 2) and stomach (NNT = 3) ulceration; and resolution of dyspepsia (NNT = 13).3 Non-invasive Helicobacter pylori diagnostic tests are available for point-of-care (POC) use in primary care and include IgG serology, 13C-urea breath test (UBT), and monoclonal stool antigen. Only UBT is sufficiently accurate to confirm current infection or eradication.4 HP IgG serology cannot differentiate current from past infection. Rapid qualitative stool antigen testing currently lacks diagnostic accuracy. Isotope ratio mass spectrometry (IRMS) is the most commonly used 13C-UBT method in the UK. However, the sampling procedure involves many opportunities for test incompletion: collection of the test from the pharmacy; returning for a subsequent extended appointment; sending the test to the laboratory; and awaiting results. In comparison, non-dispersive isotope selective infrared spectroscopy (NDIRS) has potential as a POC device: it can be used by non-specialist staff outside the laboratory setting, it …

O Blood Group as a Risk Factor for Helicobacter Pylori IgG Seropositivity Among Pregnant Sudanese Women.

The objective was to investigate the prevalence and the association between blood groups and Helicobacter pylori IgG seropositivity among pregnant Sudanese women. A cross-sectional survey was carried-out at Saad Abul Ela Maternity Hospital, Khartoum, Sudan during the period of July 2014 through December 2015. Questionnaires covering socio-demographic and obstetrics information were administered. Specific H. pylori IgG antibody was analysed using ELISA. One hundred eighty six pregnant women were enrolled. The mean (SD) of the age, parity was 28.3 (2.6) years and 2.6 (3.5), respectively. Of the 186 women, 42 (22.6%), 24 (12.9%), 11(5.9%) and 109 (58.6%) had blood group A, B, AB and O, respectively. H. pylori IgG seropositivity rate was 132/186 (71.0%). There was no significant difference in age and parity between women with H. pylori IgG seropositive and seronegative. Compared with the women with H. pylori IgG seronegative, significantly higher numbers of women with H. pylori IgG seropositive had O blood group, [84/132(63.6) versus 25/54(46.3), P<0.001]. In binary logistic regression, women with O blood group (OR= 2.084, 95% CI=1.060-4.097, P=0.033) were at a higher H. pylori IgG seropositivity. The current study showed that women with blood group O were at higher risk for H. pylori IgG seropositivity.

Mental vulnerability, Helicobacter pylori, and incidence of hospital-diagnosed peptic ulcer over 28 years in a population-based cohort

Objective: To examine whether mental vulnerability, an enduring personality characteristic, predicts incident hospital-diagnosed ulcer over three decades.Materials and methods: A population-based cohort study enrolled 3365 subjects with no ulcer history, ages 30–60, in 1982–3. Mental vulnerability, Helicobacter pylori IgG antibodies, socioeconomic status, and sleep duration were determined at baseline; non-steroidal antiinflammatory drug use, smoking, leisure time physical activity, and alcohol consumption both at baseline and in 1993–4. Hospital diagnoses of incident ulcer through 2011 were detected using the Danish National Patient Registry.Results: Ulcers were diagnosed in 166 subjects, including 83 complicated by bleeding or perforation. Age-, gender-, and socioeconomic status-adjusted associations were significant for mental vulnerability (Hazard Ratio (HR) 2.0, 95% Confidence Interval 1.4–2.8), Helicobacter pylori (HR 1.7, CI 1.2–2.3), smoking (HR 2.0, CI 1.3–3.1), heavy drinking (HR 1.6, CI 1.1–2.4), abstinence (HR 1.6, CI 1.1–2.5), non-steroidal antiinflammatory drugs (HR 2.1, CI 1.5–3.0), and sedentary lifestyle (HR 1.9, CI 1.4–2.7). Adjusted for all behavioral mediators, the HR for mental vulnerability was 1.5 (CI 1.0–2.2, p = .04). Mental vulnerability raised risk in Helicobacter pylori seropositive subjects and those exposed to neither Helicobacter pylori nor non-steroidal antiinflammatory drugs; its impact was virtually unchanged when analysis was limited to complicated ulcers.Conclusions: A vulnerable personality raises risk for hospital-diagnosed peptic ulcer, in part because of an association with health risk behaviors. Its impact is seen in ‘idiopathic’ and Helicobacter pylori-associated ulcers, and in acute surgical cases.

Effect of Helicobacter pylori on metabolic syndrome parameters in diabetic patients.

The aim of this study is to survey the effect of Helicobacter Pylori on metabolic syndrome parameters in diabetic patients. Helicobacter pylori (HP) infection is the most common infection in developing countries. Some studies showed the association between HP infection and insulin resistance. Insulin resistance is a major mechanism in the development of metabolic syndrome (MetS) and it is said that MetS is more prevalent among HP infected subjects. Also, some studies have shown that MetS is common among patients with type 2 diabetes mellitus. In this study, we aimed to investigate the prevalence of MetS in diabetic patients and its association with HP. This cross-sectional study was carried out from May to December 2014 on 211 diabetic patients. For each patient, the following data were collected: age, gender, diabetes duration, weight, body mass index (BMI), waist circumference, blood pressure (BP), HDL, cholesterol, triglyceride (TG), total cholesterol, and HbA1c. The lipid profile was performed on fasting samples. Anti- HP IgG antibody was measured and serum titer >30AU/mL was considered positive. MetS was diagnosed by The National Cholesterol Education Program's Adult Treatment Panel III report (NCEP-ATPIII) and IDF criteria. Totally 139 patients (65.9%) were HP+ and 72 patients (34.1%) were HP-. Age, gender and diabetes duration were not significantly different in both groups. BMI was significantly lower in HP+ women (29.05±5.26 vs. 31.45±4.8, p=0.02). Although the waist circumference of men was not different between the two groups but it was significantly lower in HP+ women (102.04±12.37 vs. 97.3±10, p=0.03). Although BP and TG levels were not statistically different in HP+ and HP- patients, but HP+ patients had lower HDL level (p=0.037) which was due to lower HDL in men (58.2±26.6 vs. 72.48±28.1, p=0.012). The prevalence of MetS according to the IDF criteria among HP+ and HP- patients was 76.6% vs. 69.8% (p=0.27). Also, the prevalence of MetS according to NCEP-ATP III criteria among HP+ and HP- patients was 90.4% vs. 87.2% (p=0.5). Duration of diabetes did not affect the prevalence of metabolic syndrome among HP+ and HP- patients. It seems that HP infection increases the prevalence of metabolic syndrome through an increase in insulin resistance. According to NCEP-ATPIII criteria, the increase in the prevalence of metabolic syndrome in HP+ patients is almost significant, however more complete studies is recommended to investigate this relationship.

Helicobacter pylori seroprevalence in children with sleep-disordered breathing

ObjectiveChronic Helicobacter pylori (HP) infection is considered to be a factor involved in obstructive sleep apnea syndrome (OSAS). This cross-sectional study examined the seroprevalence of HP in children with sleep-disordered breathing (SDB) in respect to OSAS severity and in reference to other common pediatric medical conditions. MethodsOvernight polysomnography with pH-metry (PSG) was performed at a Sleep Laboratory (in the years 2008–2011). OSAS severity was determined based on Obstructive Apnea Index (OAI). Subjects were classified into primary snoring group (OAI < 1/hours), mild - moderate OSAS (OAI: 1–5/hour), and severe OSAS (OAI: ≥5/hour). HP IgG was tested by an enzyme-linked immunosorbent assay in the SDB (n = 115) and reference (n = 387) groups [reference group consisted of 4 subgroups based on ICD-10 diagnoses encompassing conditions affecting the skin, respiratory system, food hypersensitivity, and gastrointestinal tract]. Analyses were performed by nonparametric statistical tests. ResultsHP seropositivity was 10.4% (12/115) in the SDB group and 11.6% (45/387) in the reference group. HP positive and negative subjects did not differ in PSG, acid gastro-esophageal reflux index nor in age, sex, nutritional status (BMI-z score), and hematological indices in the SDB group. Seropositivity was found in 16.7% of the primary snoring group, 10.2% of mild-moderate OSAS, and in 11.1% of severe OSAS (chi2 p = 0.832). ConclusionsChildren with SDB are not more predisposed to a chronic HP infection than children with other common chronic pediatric conditions. HP seropositivity does not influence OSAS severity but possible infection should none-the-less be considered on a case-by-case basis.

Detection of Helicobacter pylori IgG and IgM Antibodies in Iraqi Dyspeptic Patients

This study is conducted to detect H. pylori IgG and IgM antibodies by different serologicaltechniques (Immunochromatography test for rapid H. pylori IgG antibodies detection andenzyme linked immunosorbant assay test for H. pylori IgM antibodies detection). One hundredand twenty five dyspeptic patients are subjected to esophageal gastroduodenoscopy at theEndoscopy Unit at Baghdad Teaching Hospital from 10/1/2013 to 8/1/2014. Venous bloodsamples are collected from each patient. This study recorded, (89%) sensitivity for each of IgMTest by ELISA kit and Rapid IgG Test by Immunochromatography kit, but Rapid IgG Test hasmore specificity (85.64%) than IgM Test (80.8%).The higher prevalence 31.2% of H. pylori IgMantibodies observed among age group (21-40) years, whereas the IgM showed a slight rise 2.4%from the (10-20) years age group as acute H. pylori infection, although statistically there is a nonsignificant (P ≥0.05) association between H. pylori IgM antibodies and gender, but the females(32%) were more affected than males (27.2%). Also recorded significant (P ≤0.05) associationbetween chronic H. pylori infection and gender (male 45% and female 55%), and highlysignificant association of blood groups phenotypes and Rh factor with H. pylori infectionrecorded by rapid IgG test (P ≤0.01), as the seropositivity of H. pylori according to rapid IgG testwas (35, 15, 0 and 50) % for (A, B, AB and O) patients respectively, and (85and 15) % for Rh+and Rh- patients, respectively.

Prevalence of Helicobacter pylori among dyspeptic patients in a tertiary care centre in South Kerala, India

Background: Helicobacter pylori (HP) is the most common bacterial infection in humans. It affects > 50% of world population and remains the major aetiological agent of ailments of upper gastrointestinal (GI) tract of humans. HP eradication prevents pre-neoplastic changes of gastric mucosa and regression of gastric mucosa-associated lymphoid type lymphoma. For these reasons and many others, it is worthwhile testing patients with dyspepsia for HP, who might benefit from eradication therapy. Objective : To study the prevalence of HP infection among dyspeptic patients in a tertiary care centre in South Kerala. Materials and Methods: Gastric biopsy specimens were collected from referred patients with dyspepsia from Southern districts of Kerala (Thiruvananthapuram, Kollam and Pathanamthitta) attending the Gastroenterology Outpatient Department, who underwent upper GI endoscopy, during a one-year period from December 2009 to November 2010. The serum samples were collected for doing HP IgG enzyme-linked immunosorbent assay test. The biopsy specimens were used for doing rapid urease test (RUT) and culture was done in patients showing urease positivity and/or high level of IgG antibody titre. Results: The study was conducted on 250 consecutive dyspeptic patients, with an age range of 7-73 years. Significant IgG titres (>37.5 IU/ml) were detected in 89 cases (35.6%), while the RUT was positive in 30 cases (12%). Subjects were considered positive for HP infection by combined IgG antibody and/or urease test positivity and negative when both the tests were negative. In this study, ninety patients (36%) were positive for HP infection, and 160 (64%) were negative. Culture was done in patients showing urease positivity and/or high level of IgG antibody titre and proportion of culture positivity among this group was 34%. Conclusion: Seroprevalence of HP infection in South Kerala was found to be low (35.6%). Low infection rates were seen in children, similar to that in developed countries, and it is likely that the prevalence of HP may fall in the coming years. Due to the lower percentage of HP infection in this area, a test and treat policy is applicable as in many developed countries.

Elderly women who received Helicobacter pylori-eradicating therapy have reduced risk of low skeletal muscle mass.

BackgroundSarcopenia is associated with adverse outcomes such as physical disability, poorer quality of life, and death. Helicobacter pylori (HP) eradication increases ghrelin secretion, which may be a possible treatment for sarcopenia. We investigated whether HP eradication reduces the risk of low muscle mass (LMM), which is an integral component of sarcopenia.Materials and methodsHealthy, asymptomatic women aged ≥60 years who participated in a health screening program were enrolled. Subjects with a history of HP eradication were compared with those who were HP IgG+, but had not received HP-eradicating therapy. Body composition was measured by multifrequency bioelectrical impedance analysis. LMM was defined as body muscle mass 2 standard deviations below the mean muscle mass of healthy women aged 20–39 years from the same program. Multivariable analysis was used to identify sarcopenia risk factors.ResultsThree hundred seventy-two women had received HP eradication, while 689 HP IgG+ women had not. The prevalence of LMM was significantly lower in those who received HP eradication (13.7% vs 21.6%, P=0.002). Multivariable analysis identified risk factors for LMM as age, white blood cell count, serum total protein concentration, and the metabolic syndrome. HP eradication (odds ratio: 0.632, 95% confidence interval: 0.440–0.824, P=0.013) was a significant preventive factor, and exercise (odds ratio: 0.710, 95% confidence interval: 0.504–1.002, P=0.051) had a preventive tendency.ConclusionHP eradication might reduce LMM risk. This finding should be confirmed in prospective longitudinal studies to determine the long-term effects of HP eradication on sarcopenia.

242 A Combination of Fasting Serum Gastrin Concentration, Pepsinogen 1/2 Ratio and Helicobacter pylori IgG Antibody Serotype Accurately Predicts Gastric Mucosal Preneoplasia in a Large European Cohort

242 A Combination of Fasting Serum Gastrin Concentration, Pepsinogen 1/2 Ratio and Helicobacter pylori IgG Antibody Serotype Accurately Predicts Gastric Mucosal Preneoplasia in a Large European Cohort

Association of Helicobacter pylori IgG Antibody with Microvascular Complications in Type II Diabetic Patients

Association of Helicobacter pylori IgG Antibody with Microvascular Complications in Type II Diabetic Patients

The Correlation of Infectious and Inherent Agents with Acute Myocardial Infarction in Patients of Teaching Hospitals in Qazvin in 2013

Background: Acute myocardial infarction (AMI) is one of the most common reasons of hospitalization in western countries. The rate of early mortality (the first 30 days) caused by AMI is about 30%. Many studies have introduced the infection with Helicobacter pylori and mycoplasma pneumonia as causes of AMI. However, some other studies on the effect of mycoplasma pneumonia, Helicobacter pylori, and blood group on the incidence of heart attack showed contradictions that might be due to the ethnical and racial heterogeneities. Therefore, this study was conducted to determine the correlation of the infection with Helicobacter pylori and mycoplasma pneumonia and blood group with AMI in Qazvin Province, Iran. Objectives: In this respect, the infection with Helicobacter pylori and mycoplasma pneumonia can be taken as a risk factor for AMI thereafter if there is a strong correlation between infection with Helicobacter pylori and mycoplasma pneumonia and AMI, and also the high-risk blood groups can be identified, and consequently, risk factors for AMI be reduced through early treatment. Materials and Method: This case-control study was performed in teaching hospitals in Qazvin. After completing the sample size in both groups (blood serum of 90 patients with a history of infarction as the case group and blood serum of 90 other people that matched for age and sex with the first group and had no history of infarction as the control group), the studies were performed to determine the correlation of AMI with mycoplasma pneumonia through the level of IgG antibody, with Helicobacter pylori through the level of relevant antibodies (IgA and IgG), and with blood groups through back-type method. Results: Men and women of the case group comprised 63.3% and 36.7%, respectively, and men and women of the control group comprised 67.8% and 32.2%, respectively. The level of Helicobacter pylori IgG was considered negative in 1.1%, borderline in 5.6%, and positive in 93.3% in the case group and negative in 5.6%, borderline in 10%, and positive in 84.4% in the control group. According to the Chisquare test, the difference between the two groups was significant (P Helicobacter pylori IgA was negative in 20%, borderline in 26.7%, and positive in 53.3% in the case group and negative in 12.2%, borderline in 20%, and positive in 67.8% in the control group. Based on the Chisquare test, the difference between the two groups in this regard was not significant (P > 0.05). The level of mycoplasma pneumonia IgG in the samples was negative in 17.8%, borderline in 5.6%, and positive in 76.7% in the case group and negative in 27.8%, borderline in 2.2%, and positive in 70% in the control group. Based on the Chisquare test, there was no significant difference between the two groups in this regard (P > 0.05). The results on testing the ABO blood groups showed that 23.3%, 21.1%, 27.8%, and 27.8% of the patients with AMI in the case group had the blood group O, AB, A, and B, respectively, and 30.7%, 13.6%, 34.1%, and 21.6% of the people in the control group had the blood group O, AB, A, and B, respectively. The analysis of results did not show a significant difference between case and control group in terms of ABO blood groups (P > 0.05). Conclusion and Recommendation: This study did not show a significant difference between the two groups in terms of the level of IgG except infection with Helicobacter pylori. However, more studies should be performed at local and national levels in order to find the role of risk factors of AMI in each region.

Associated factors of atrophic gastritis diagnosed by double-contrast upper gastrointestinal barium X-ray radiography: a cross-sectional study analyzing 6,901 healthy subjects in Japan.

BackgroundDouble-contrast upper gastrointestinal barium X-ray radiography (UGI-XR) is one of the most widely conducted gastric cancer screening methods. It has been executed to find gastric cancer, but has not been usually executed to detect premalignant atrophic mucosa of stomach. To understand the meaning of UGI-XR-based atrophic gastritis, we analyzed its association with several causative factors including Helicobacter pylori (HP) infection.MethodsWe evaluated 6,901 healthy adults in Japan. UGI-XR-based atrophic gastritis was diagnosed based on the irregular shape of areae gastricae and its expansion in the stomach.ResultsOf the 6,433 subjects with no history of HP eradication and free from gastric acid suppressants, 1,936 were diagnosed as UGI-XR-based atrophic gastritis (mild: 234, moderate: 822, severe: 880). These were univariately associated with serum HP IgG and serum pepsinogen I/II ratio with statistical significance. The multiple logistic analysis calculating standardized coefficients (β) and odds ratio (OR) demonstrated that serum HP IgG (β = 1.499, OR = 4.48), current smoking (β = 0.526, OR = 1.69), age (β = 0.401, OR = 1.49), low serum pepsinogen I/II ratio (β = 0.339, OR = 1.40), and male gender (β = 0.306, OR = 1.36) showed significant positive association with UGI-XR-based atrophic gastritis whereas drinking and body mass index did not. Among the age/sex/smoking/drinking-matched 227 pairs derived from chronically HP-infected and successfully HP-eradicated subjects, UGI-XR-based atrophic gastritis was detected in 99.1% of the former but in only 59.5% of the latter subjects (p<0.0001). Contrastively, UGI-XR-based atrophic gastritis was detected in 13 of 14 HP-positive proton pump inhibitor users (92.9%) and 33 of 34 HP-positive histamine H2-receptor antagonist users (97.1%), which are not significantly different from gastric acid suppressant-free subjects.ConclusionsThe presence of UGI-XR-based atrophic gastritis is positively associated with Helicobacter pylori infection, current smoking, age, decreased serum pepsinogen I/II ratio, and male gender. Eradication of Helicobacter pylori seems to superficially improve UGI-XR-based atrophic gastritis whereas intake of gastric acid suppressants does not.

&lt;i&gt;HELICOBACTER&lt;/i&gt; INFECTION AND ALLERGY IN MAJORITY AND ROMA POPULATION IN THE CZECH REPUBLIC

In our paper we decided to evaluate the hypothesis about the relation between living conditions, infection with Helicobacter pylori and development of allergic problems. Two groups of children, one from the Roma population and one from the majority population, were chosen for this study. Comparing the levels of total IgE and specific IgE antibodies, IgA and IgG Helicobacter pylori antibodies in both populations, we found significant differences in all tested aspects. We conclude that changes in lifestyle together with changes in improvements in living conditions and reduction of risk factors can significantly influence the health conditions of thepopulation and overall quality of life.

Evaluation of the relationship between preeclampsia and seropositivity of infectious disease in maternal plasma.

The aim of the present study was to investigate the association between maternal infections and preeclampsia. Fifty-four pregnant women with preeclampsia (study group) and 54 healthy pregnant women (control group) were enrolled in this study. Chlamydia pneumoniae (CP), Mycoplasma hominis (MH), Helicobacter pylori (HP), Cytomegalovirus (CMV), Herpes simplex virus type 2 (HSV-2), and Toxoplasma gondii (TG) antibodies were analyzed in the maternal plasma of all of the participants. There was no difference in the demographic data between groups. Maternal plasma seropositivity for CP IgM; MH IgA and IgG; HP IgM, IgA and IgG; TG, CMV and HSV-2 IgM and IgG were not significantly different between the preeclampsia and control groups. The seropositivity for CP IgG was significantly higher in women with preeclampsia versus the control women. Our results suggested that preeclampsia is associated with CP IgG seropositivity.