Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – EU funding. Main funding source(s): This publication is part of a project that has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 954783. Background Stereotactic arrhythmia radio-ablation (STAR) is a promising strategy for the non-invasive treatment of ventricular arrhythmias (VA). The management of the cardio-respiratory motion of the target and the reduction of the uncertainties related to patients’ positioning are two of the main challenges that STAR has to overcome. A prototype of a system was developed that can automatically acquire and interpret echocardiographic images using an artificial intelligence (AI) algorithm to calculate cardiac displacement in real-time. Purpose To evaluate the feasibility of this automatic acquisition system in patients with a history of VA. Methods We conducted a single center, feasibility study enrolling consecutive patients with a history of VA. Echocardiographic images were automatically acquired from the parasternal and apical views with a dedicated probe. The system was designed to hold the probe fixed to the chest in the supine position during both free-breathing and short expiratory breath-hold sequences, to simulate STAR treatment. The primary endpoint was the percentage of patients reaching a score ≥2 in a multi-parametric assessment evaluating the quality of automatically acquired images in terms of: A - allowing a proper identification of the cardiac cycle phase using the AI algorithm, B - allowing a correct measurement of the heart displacement by the AI algorithm, C - ability to visually distinguish typical cardiac structures, and D - stability of the image throughout the respiration cycle. Moreover, we investigated the potential impact of clinical and demographic characteristics on achieving the primary outcome. Results From May to September 2021, we enrolled 24 patients (63±14 years, 21% females). All of them had a history of VA and 21 (88%) had an ICD. Eight patients (33%) had coronary artery disease, 12 (50%) had non-ischemic cardiomyopathy, and 3 had idiopathic VA. Parasternal as well as apical images were obtained from all patients except from one, in whom parasternal view could not be collected due to the patient’s inability to maintain the supine position. The primary outcome was achieved in 23 patients (96%) for the apical view, in 20 patients (87%) for the parasternal view, and in all patients in at least one of the two views. The images quality was maximal (i.e. score=4) in at least one of the two windows in 19 patients (79%). Atrial fibrillation arrhythmia was the only clinical characteristics associated with a poor score outcome in both imaging windows (apical p=0,022, parasternal p=0,014). Conclusion An automatic ultrasonographic image acquisition system associated with an AI algorithm is feasible for real-time monitoring of cardiac motion in patients with a history of VA. The possibility of real-time, non-invasive monitoring of cardiac position would lead to a significant improvement in the quality and safety of STAR treatment, particularly in case of treatment with heavy particles such as protons and carbon ions.