In this study, the heavy metal accumulation Arsenic, Beryllium, Boron, Cadmium, Chromium, Cobalt, Copper, Iron, Lithium, Lead, Manganese, Nickel, Rubidium, Strontium, Uranium, Vanadium and Zinc (As, Be, B, Cd, Cr, Co, Cu, Fe, Li, Pb, Mn, Ni, Rb, Sr, U, V and Zn) in the muscle and liver tissues of Cyprinus carpio fish caught from three different regions of Büyük Menderes River were investigated. Average concentrations of heavy metals in fish muscle tissue samples collected from each region investigated in mg/l were found as As: 0.0267±0.01314, Be: 1.5654±0.04758, B: 1.7020±0.08111, Cd: 0.0944±0.01386, Cr: 1.3272±0.09183, Co: 0.0949±0.02840, Cu: 1.4317±0.25331, Fe: 23.4205±1.55185, Li: 0.2579±0.00933, Mn: 0.3203±0.02315, Ni: 0.6651±0.06915, Pb: 0.4472±0.02485, Rb: 1.4729±0.22366, Sr: 1.1816±0.5771, , U: 0.2225±0.0659, V: 26.0355±1.04913, Zn: 12.5085±1.16400, and the levels of Li, Be, B, Cr, Co, Rb, Cd, V and U were higher than the limit levels. Li, Cr, Mn, Fe, Ni, Zn, Pb, Cd, V and U heavy metals in liver; Be, B, Co, Rb, Sr and As heavy metals were found to be higher in muscle tissue. Results demonstrate that the third region has the highest levels of heavy metal pollution among the three regions investigated. In addition to that, the source of the heavy metal toxicity determined in the second region was found as the third region. According to obtained results, human consumption of fish caught in all three region may be risky to health.