We discuss the constraint on the size of QCD instanton effects in low-energy effective theory. Among various instanton effects in meson mass spectrum and dynamics, we concentrate on the instanton-induced masses of light quarks. The famous instanton-induced six-quark interaction, so-called ’t Hooft vertex, could give nonperturbative quantum corrections to light quark masses. Many works have already been achieved to constrain the mass corrections in light meson system, or the system of [Formula: see text], [Formula: see text], [Formula: see text] and [Formula: see text], and now we know for a fact that the instanton-induced mass of up-quark is too small to realize the solution of the strong CP problem by vanishing current mass of up-quark. In this work, we give a constraint on the instanton-induced mass correction to light quarks from the mass spectrum of heavy mesons, [Formula: see text], [Formula: see text], [Formula: see text] and their antiparticles. To accomplish this, the complete second-order chiral symmetry breaking terms are identified in heavy meson effective theory. We find that the strength of the constraint from heavy meson masses is at the same level of that from light mesons, and it would be made even stronger by more precise data from future [Formula: see text] factories and lattice calculations.
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