Heavy Alcohol Consumption Research Articles

Long-Term Survival Analysis of 5619 Total Ankle Arthroplasty and Patient Risk Factors for Failure.

Total ankle arthroplasty (TAA) has higher complication and failure rates compared to other surgical joint replacement procedures despite technological advances. This study aimed to find the long-term survivability of the TAA procedure and identify the patient risk factors for failure with one of the largest cohorts of patients in the literature. This retrospective cohort study involving cases between 2007 and 2018 analyzed patients who received an index primary TAA procedure in Korea. A total of 5619 cases were included in the final analysis. The TAA failure was defined as either a case with revision arthroplasty or a case with TAA implant removal and arthrodesis performed after primary TAA. During the study period, the 5-year survival rate was 95.4% (95% CI, 94.7-96.1%), and the 10-year survival rate was 91.1% (95% CI, 89.1-93.1%). A younger age (<55 years, adjusted hazard ratio [AHR], 1.725; 55-64 years, AHR, 1.812; p < 0.001 for both), chronic pulmonary disease (AHR, 1.476; p = 0.013), diabetes (AHR, 1.443; p = 0.014), and alcohol abuse (AHR, 1.524; p = 0.032) showed a significantly high odds ratio for primary TAA failure in Cox regression analysis. The 10-year TAA survivorship rate was 91.1%. A younger age, chronic pulmonary disease, diabetes, and heavy alcohol consumption are risk factors for TAA.

Discrimination and social identity processes predict impairment and dysfunction among heavy drinkers

BackgroundPrevious research has linked discrimination to poorer health. Yet health risk behaviours such as heavy alcohol consumption are often targeted with stigmatising public health campaigns. The current study sought to establish the link between experiencing discrimination and health outcomes among heavy drinkers, with a focus on exploring the multiple social identity processes that might underpin this relationship. MethodsA survey was conducted with 282 people who self-reported consuming alcohol above recommended guidelines. We measured discrimination experienced as a drinker, components of social identification as a drinker (centrality, satisfaction, solidarity, homogeneity, and self-stereotyping), and two health outcomes: psychological distress and severity of alcohol use disorder symptomatology. ResultsDiscrimination was a moderate-large predictor of psychological distress and alcohol use disorder symptoms. Three social identity constructs were implicated in the link between discrimination and ill-health: identity centrality and homogeneity positively mediated this relationship while identity satisfaction was a negative mediator. The model explained a large proportion of the variance (39–47%) in health outcomes. DiscussionResults are interpreted with an emphasis on the need to avoid stigmatising messaging and to prioritise social identity processes to prevent and treat substance use disorders. We further highlight the need for social identity researchers to consider the multidimensional nature of social identities, especially in the context of stigmatised groups.

Hydrogen gas alleviates acute ethanol-induced hepatotoxicity in mice via modulating TLR4/9 innate immune signaling and pyroptosis

Alcoholic liver disease (ALD), which is induced by chronic heavy alcohol consumption, accompanies complicated pathological mechanisms, including oxidative stress, inflammation, cell death, epigenetic changes and acetaldehyde-mediated toxicity. Hydrogen (H2) is the lightest gas with multiple biological effects such as high selective anti-oxidation, anti-inflammation and anti-apoptosis. However, the dose effects and innate immune mechanisms of intraperitoneal injection of H2 on ALD are limited. Here, we used acute ethanol-induced hepatotoxicity mice models to estimate the actions of intraperitoneal injection of H2 on ALD. The effects of H2 on acute ethanol-induced liver damage were examined by hepatic oil red O staining, quantitative PCR (qPCR) for lipid metabolic genes, hepatic triglyceride (TG) and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Hepatic mitochondrial superoxide (MitoSOX), 3-nitrotyrosine (3-NT), malondialdehyde (MDA), and glutathione (GSH) levels were examined to evaluate oxidative stress. Immunoblot, and immunofluorescence staining were used to further confirm the innate immune molecular targets of H2. Our results showed that intraperitoneal injection of H2 improved acute ethanol-induced liver injury in mice in a dose dependent manner, as indicated by decreasing serum ALT and AST levels, hepatic TG levels, and increasing lipid export genes (Mttp and Apob) mRNA levels and reducing fatty acid uptake gene (CD36) mRNA levels. Mechanistically, H2 inhibited hepatic oxidative stress as indicated by reducing reactive oxygen species (ROS), 3-NT, and MDA levels in the liver, while increasing hepatic GSH levels; inhibited the overactived TLR4/9-NF-κB-TNF-α/IL-1β/IL-18 innate immune signaling; suppressed the canonical Caspase-1-GSDMD pyroptosis signaling, and the non-canonical pyroptosis signaling, such as Caspase-11-GSDMD, Caspase-8-GSDMD and Caspase-3-GSDME signaling. Therefore, our study highlights that intraperitoneal injection of H2 may represent a novel therapeutic and safe strategy for ALD via modulating oxidative stress, innate immunity and pyroptosis.

Maternal Prenatal Use of Alcohol, Tobacco, and Illicit Drugs and Associations with Childhood Cancer Subtypes.

The association between childhood cancer risk and maternal prenatal substance use/abuse remains uncertain due to modest sample sizes and heterogeneous study designs. We surveyed parents of children with cancer regarding maternal gestational use of tobacco, alcohol, and illicit drugs, using a Likert-type scale, and demographic, perinatal, and clinical variables. Multivariable log-Poisson regression assessed differences in frequency of prenatal substance use across fifteen childhood cancer subtypes, adjusting for birthweight, gestational age, and demographic factors. Respondents from 3,145 unique families completed the survey (92% biological mothers). A minority reported gestational use of tobacco products (14%), illicit drugs including marijuana or cocaine (4%), or more than a moderate amount of alcohol (2%). Prenatal illicit drug use was associated with increased prevalence of intracranial embryonal tumors [prevalence ratio (PR) = 1.94; confidence interval [CI], 1.05-3.58], including medulloblastoma (PR = 1.82) and supratentorial primitive neuroectodermal tumors (PNET; PR = 2.66), and was also associated with retinoblastoma (PR = 3.11; CI, 1.20-8.08). Moderate to heavy alcohol consumption was strongly associated with elevated prevalence of non-Hodgkin lymphoma (PR = 5.94; CI, 1.84-19.21). Prenatal smoking was not associated with elevated prevalence of any childhood cancer subtype. We identify novel associations between illicit drug use during pregnancy and increased prevalence of nonglioma central nervous system tumors, including medulloblastoma, supratentorial PNETs, and retinoblastoma. Gestational exposure to alcohol was positively associated with non-Hodgkin lymphoma. Although alcohol and tobacco use during pregnancy has declined, gestational cannabis use has risen. Investigating its impact on neurodevelopment and brain tumorigenesis is vital, with important implications for childhood cancer research and public health education.

Association between psychosocial factors and co-morbid cigarette smoking and alcohol use in a population experiencing homelessness

The prevalence of combustible cigarette smoking in populations experiencing homelessness in the United States is five times that of the general population. The psychosocial well-being of persons who smoke and experience homelessness is poorer if such persons also use alcohol heavily. The PTQ2 study was a randomized clinical trial among persons experiencing homelessness who were also current smokers and heavy alcohol consumers. Secondary data analysis of the PTQ2 baseline data was conducted to examine associations among psychosocial variables (anxiety, depression, hopelessness, social network size), heaviness of smoking (cigarettes/day) and alcohol consumption (drinking days/month), and duration and frequency of homelessness. Among the 420 participants, the majority were male (75%), black (70%) and non-Hispanic (94%) with a mean age of 46.6 years (SD = 11.6). Bivariate analyses show that heaviness of smoking was positively correlated with social network size (r = 0.16, p = .001). Heaviness of drinking was positively correlated with the MINI anxiety score (r = 0.13, p = .009) and marijuana use (median total number of drinks in past 30 days among those who used marijuana in past 30 days vs. did not use: 50 vs. 24, p < .0001), and associated with frequency of homelessness (median total number of drinks in past 30 days among those experiencing homelessness once vs. >1 time: 30 vs. 44, p = .022). The findings highlight the psychosocial factors that warrant consideration when addressing heavy smoking and alcohol consumption in persons experiencing homelessness.

The Moderating Role of Stress Mindset on the Relationship between Drinking to Cope and Alcohol Consumption among Undergraduate College Student Drinkers

Objective Previous research suggests drinking alcohol to cope with negative affect, including stress, is a risk for increased alcohol consumption and alcohol-related problems. Stress mindset, the individually held belief that stress can lead to either enhancing or debilitating outcomes, has yet to be studied within the context of alcohol use. Studying stress mindset among college students as it relates to alcohol consumption may provide important insight into heavy alcohol use in this population. Method A sample of 320 undergraduates (M age = 19.06 (SD = 0.06); 63.44% female; 65.49% White) who endorsed past-year alcohol use completed self-report measures of drinking motives, stress mindset, alcohol consumption, and alcohol-related consequences. Zero-inflated negative binomial regressions were utilized to examine the moderating effect of stress mindset on the relationship between drinking to cope and alcohol consumption. Results Stress mindset significantly moderated the relationship between drinking to cope and alcohol consumption (IRR = 0.98, se = 0.01, p < 0.05, CI = 0.96, 1.00), such that the relationship was stronger among those with a debilitating stress mindset compared to those with an enhancing stress mindset. Stress mindset did not significantly moderate the relationship between drinking to cope and alcohol-related consequences. Conclusions Individuals with high drinking to cope scores and who hold a debilitating stress mindset may be at a particular vulnerability for heavy alcohol consumption. The present study furthers our understanding of predictors of alcohol use in a college sample and suggests the importance of future research focused on stress mindset among college student drinkers.

A case study on Ayurvedic management on Kamala w.s.r. to Hepatitis E Virus

Hepatitis is defined as liver inflammation that can be caused on by a number of conditions, including prolonged drug use, heavy alcohol consumption, autoimmune conditions, and exposure to toxins. The term "viral hepatitis" describes the most common type of hepatitis, which is caused by an infection with a virus. In this case, report effect of Ayurveda intervention in HEV is reported. A 55-year, old non-alcoholic, married male patient from an urban area, presented with yellowish discolouration of sclera, urine, upper and lower extremities, reduced appetite, nausea, fatigue with generalized weakness and experiencing upper abdominal pain to the National Institute of Ayurveda Hospital, Jaipur, Rajasthan. On examination, icterus was present, pitting edema present on both lower legs. Hematological examination revealed altered total bilirubin, SGOT, SGPT, Triglycerides, ESR, CRP. Also, sonographic examination revealed a mild hepatomegaly in the patient. The total duration of the treatment was 2 months. The patient was assessed for improvement in hematological parameters, viral markers, and signs and symptoms. after two months, significant improvement was observed in signs and symptoms, and all altered hematological parameters, and viral markers were within normal limits. Hence, presenting this case is evidence to demonstrate the effectiveness of two months of Ayurvedic treatment in managing HEV. The total duration of the treatment was 60 days.

Non-Adherence to Antihypertensive Medications Among the US Population: A Pooled Analysis of 22 Million Patients

BackgroundNon-adherence is a barrier to the control of hypertension. This study aimed to analyze the factors and interventions affecting adherence rates in the US. MethodsPubMed, Scopus, Web of Science, and Embase were searched on January 21 st, 2022 for studies on the adherence to anti-hypertensives in the US. R software (4.2.1) and RevMan (5.4) were used for the analysis. ResultsOur analysis showed that non-adherence to antihypertensives was higher in Hispanics compared with Blacks (OR= 1.25, p-value= 0.0335) with further analysis showing lower non-adherence rates among Whites compared with Blacks (OR= 0.63, p-value= 0.0049). Younger individuals had higher non-adherence rates (53%) compared with individuals older than 60 (46%), however, no statistically significant difference was detected between both groups. Unemployment was associated with decreased adherence (OR= 0.40, p-value< 0.00001), while gender and marital status yielded insignificant associations. Patients who suffered from multiple comorbidities (OR= 0.43, p-value= 0.004), depression (OR= 0.65, p-value <0.00001), and poor mental health (OR= 0.38, p-value< 0.00001) had lower adherence rates. Heavy alcohol consumption was associated with a decline in adherence, conversely, obesity didn't significantly affect the adherence rates. Regarding interventions, Fixed-dose combination had lower non-adherence rates compared with the control group (OR= 0.83, p-value= 0.0029), while telepharmacy didn't prove to be as effective. Lastly, exercise was associated with higher adherence (OR= 3.08, p-value< 0.00001). ConclusionRace and unemployment play a role in adherence to anti-hypertensives. Patients who suffer from depression, multiple comorbidities, and heavy alcohol intake had lower adherence rates. Accordingly, interventions such as fixed-dose combinations and exercise should be targeted at susceptible groups.

Cancer Risk According to Alcohol Consumption Trajectories: A Population-based Cohort Study of 2.8 Million Korean Men.

Alcohol drinking behaviors change temporally and can lead to changes in related cancer risks; previous studies have been unable to identify the association between the two using a single-measurement approach. Thus, this study aimed to investigate the association of drinking trajectories with the cancer risk in Korean men. A trajectory analysis using group-based trajectory modeling was performed on 2,839,332 men using data on alcohol drinking levels collected thrice during the Korean National Health Insurance Service's general health screening program conducted between 2002 and 2007. Cox proportional hazards regression was performed to evaluate the associations between drinking trajectories and cancer incidence, after adjustments for age, income, body mass index, smoking status, physical activity, family history of cancer, and comorbidities. During 10.5 years of follow-up, 189,617 cancer cases were recorded. Six trajectories were determined: non-drinking, light, moderate, decreasing-heavy, increasing-heavy, and steady-heavy. Light-to-heavy alcohol consumption increased the risk for all cancers combined in a dose-dependent manner (adjusted hazard ratio [aHR] 1.03; 95% confidence interval [CI], 1.02-1.05 for light drinking, aHR 1.06; 95% CI 1.05-1.08 for moderate drinking, aHR 1.19; 95% CI, 1.16-1.22 for decreasing-heavy drinking, aHR 1.23; 95% CI, 1.20-1.26 for increasing-heavy drinking, and aHR 1.33; 95% CI, 1.29-1.38 for steady-heavy drinking [P-trend <0.001]). Light-to-heavy alcohol consumption was linked to lip, oral cavity, pharyngeal, esophageal, colorectal, laryngeal, stomach, and gallbladder and biliary tract cancer risks, while heavy alcohol consumption was associated with hepatic, pancreatic, and lung cancer risks. An inverse association was observed for thyroid cancer. The cancer risks were lower for decreasing-heavy drinkers, compared to steady-heavy drinkers. No safe drinking limits were identified for cancer risks; reduction in heavy intake had protective effects.

P59: Associations Between Smoking, Alcohol Consumption, Physical Activity and Depression in Middle-Aged Premenopausal and Postmenopausal Women

Background:Changes in lifestyle factors are known to affect mood. However, there is insufficient evidence supporting the association between smoking, alcohol consumption, physical activity and depression in middle-aged women who are likely to experience rapid hormonal changes.Methods:We used a nationwide database of medical records in South Korea. 901,721 premenopausal and 943,710 postmenopausal women aged 40 years or older included in this study. Information on smoking, alcohol consumption, physical activity was identified from health examination data and followed up for the occurrence of depression using claims data.Results:Compared with never-smokers, ex-smokers and current smokers among premenopausal and postmenopausal women showed an increased risk of depression in a dose-dependent manner (aHR 1.13 for ex-smokers; aHR 1.23 for current smokers). Compared with non-drinkers, mild drinkers showed a decreased risk of depression (aHR 0.98 for premenopausal women; aHR 0.95 for postmenopausal women), and heavy drinkers showed an increased risk of depression both among premenopausal (aHR 1.20) and postmenopausal women (aHR 1.05). The risk of depression due to smoking and heavy alcohol consumption was higher in premenopausal women than in postmenopausal women. Compared with those who had not engaged in regular physical activity, those who had engaged showed a decreased risk of depression both among premenopausal (aHR 0.96) and postmenopausal women (aHR 0.95).Conclusions:Smoking and heavy alcohol consumption increased the risk of depression, and the increased risk was prominent in premenopausal than in postmenopausal women. Regular physical activity decreased the risk of depression both in premenopausal and postmenopausal women.

A review: Alcoholic liver disease pathophysiology, current molecular and clinical perspectives

Alcohol-related liver damage (ALD) is a term used for a variety of liver conditions comprising cirrhosis, fibrosis, and steatohepatitis with chronic inflammation that is predominantly brought on by heavy alcohol consumption. ALD is currently regarded as one of the most common causes of liver disease-related mortality on a global scale. The majority of cirrhosis in the liver cases seen in district general hospitals in the UK are caused by alcohol, which is a key factor in the Western world. Alcoholic cirrhosis, acute alcoholic hepatitis, and alcoholic fatty liver (steatosis) are the three most widely recognized types of alcoholic liver disease. According to the National Institute on Alcohol Abuse and Alcoholism's the most recent surveillance report, liver cirrhosis was among the top twelve causes of death in the country, contributing to a total of 14,477 deaths in the year 2014.This page speaks about the various types of alcoholic liver damage (ALD), with a focus on cirrhosis because it is the type of liver diseases that is most frequently linked to alcohol misuse and has the most studies investigating it. Epidemiological studies have assessed the incidence of ALD and the factors who frequently cause the condition. Although excessive alcohol use is the main cause of ALD, gender and cultural variations also have a significant role in the prevalence of liver disease. Since the 1970s, cirrhosis-related mortality rates have decreased in the United States and certain other countries as well. This dip may have been caused by a variety of variables, including increasing enrollment in alcohol rehab facilities and in Alcoholics Anonymous, drops in alcohol consumption, and more

Risk factors for early-onset lung cancer in Korea: analysis of a nationally representative population-based cohort.

We examined the associations of socioeconomic factors, health behaviors, and comorbidities with early-onset lung cancer. The study included 6,794,287 individuals aged 20-39 years who participated in a Korean national health check-up program from 2009 to 2012. During the follow-up period, 4,684 participants developed lung cancer. Multivariable Cox regression analysis was used to estimate the independent associations of potential risk factors with incident lung cancer. Older age (multivariable hazard ratio [mHR], 1.13; 95% confidence interval [CI], 1.12 to 1.14) and female sex (mHR, 1.62; 95% CI, 1.49 to 1.75) were associated with increased lung cancer risk. Current smoking was also associated with elevated risk (<10 pack-years: mHR, 1.12; 95% CI, 1.01 to 1.24; ≥10 pack-years: mHR, 1.30; 95% CI, 1.18 to 1.45), but past smoking was not. Although mild alcohol consumption (<10 g/day) was associated with lower lung cancer risk (mHR, 0.92; 95% CI, 0.86 to 0.99), heavier alcohol consumption (≥10 g/day) was not. Higher income (highest vs. lowest quartile: mHR, 0.86; 95% CI, 0.78 to 0.94), physical activity for at least 1,500 metabolic equivalent of task-min/wk (vs. non-exercisers: mHR, 0.83; 95% CI, 0.69 to 0.99) and obesity (vs. normal weight: mHR, 0.89; 95% CI, 0.83 to 0.96) were associated with lower lung cancer risk, whereas metabolic syndrome was associated with increased risk (mHR, 1.13; 95% CI, 1.03 to 1.24). In young adults, age, female sex, smoking, and metabolic syndrome were risk factors for early-onset lung cancer, while high income, physical activity, and obesity displayed protective effects.

Betel Quid Chewing and Alcohol Consumption Co- Variate with Detection of Epstein - Barr Virus (EBV) DNA in Oral Squamous Cell Carcinoma Tissues: Preliminary Evidence from a Subset of an Unmatched Case-Control Study

Introduction: There is a dearth of information on the detection of oncogenic EBV in oral squamous cell carcinoma tissues in the Sri Lankan context. This subset of an unmatched case-control study aims to detect EBV-DNA in oral squamous cell carcinoma tissues of a group of Sri Lankan male patients. Method: Subset was selected representing the vast majority of OSCC patients in Sri Lanka, from an unmatched case-control study. Incisional biopsies of cases and excisional biopsies of controls were collected and stored at -800 C. DNA was extracted from frozen specimens, and Real-Time PCR was performed to detect target DNA of this ubiquitous virus, in histologically confirmed 27 Oral Squamous Cell Carcinoma (OSCC) cases and clinically diagnosed 26 Fibroepithelial-Polyp (FEP) controls with better quality DNA. Socio-demographic and substance abuse data were collected from 29 cases and 25 controls by a pretested interviewer-administered questionnaire, and the data were entered and analyzed using the SPSS-21 Statistical Package. Results: Descriptive and inferential statistics were used to determine the significance of qualitative and quantitative data statistically. The overall EBV prevalence was 34/53 (64.2%). In OSCC cases the EBV positivity was higher 21(77.8%) than the FEP controls 13 (50.0%), and this difference was statistically significant (p = 0.035). The cases were higher betel quid chewers (p = 0.003) and heavy alcohol consumers (p =0.001) than the control group. Conclusion: Betel quid chewing and alcohol consumption co-variate with the detection of Epstein-Barr virus (EBV) DNA in oral squamous cell carcinoma tissues which warrants further investigations.

Quantitative evaluation of choroidal and retinal microvasculature post-alcohol consumption: A pilot study

PurposeThe aim of this study was to assess the impact of acute, heavy alcohol consumption on the ocular microvasculature, providing insight into the largely unexplored response of microvascular structures to excessive drinking. MethodsHealthy volunteers in this prospective pilot study were tasked with consuming spirits, wine, and water at different times. Alcohol intake was measured according to body weight (g/kg). The ocular microvascular parameters primarily including choroidal volume (CV) and choroidal vessel volume (CVV) reflecting arteriolovenularity, and choroidal capillary density (CCD) reflecting capillary, were evaluated using swept-source optical coherence tomography angiography at baseline and 0.5-, 1-, 2-, and 3-hour post-consumption. ResultsA total of 34 eyes underwent 170 successful examinations in this study. After consuming spirits or wine, we observed significant decreases in CV and CVV values (all P < 0.01 for 0.5-, 1-, 2-, and 3-hour post-consumption), along with significant increase in CCD (P < 0.05 at 0.5-, 1-, 2-hour post-spirits consumption and 1-hour post-wine consumption). The most pronounced changes occurred 1-hour after spirits or wine consumption (all P < 0.001 in both univariate and multivariate model). However, post-consumption changes in the ocular microvasculature showed no significant differences between spirits and wine (P > 0.05). Additionally, no significant differences were observed in any parameters after water intake (all P > 0.05). ConclusionsExcessive alcohol consumption leads to ocular arteriolovenular vasoconstriction and capillary vasodilation, most evident 1-hour post-consumption of spirits and wine. Our research provides insight into alcohol's immediate ocular microvascular effects, hinting at systemic microvascular effects.

Knowledge of the health consequences of heavy alcohol consumption among individuals with different substance use statuses: A cross-sectional analysis of 2019 HINT Survey.

The aim of this study was to assess and compare knowledge of the health consequences of heavy alcohol consumption among individuals with different substance use statuses. We used a cross-sectional study design to analyze the Health Information National Trends Survey (HINTS) 5, Cycle 3 (2019). Participants were classified into four categories based on their substance use status: (a) nonsmoker and nondrinkers; (b) nonsmokers but drinkers; (c) smokers but nondrinkers; and (d) smokers and drinkers. Weighted logistic regression models were utilized to evaluate the association between knowledge of heavy alcohol consumption and health conditions by participants' substance use status. More than 79% of participants were aware that drinking too much alcohol causes liver disease. However, less than 40% of them realized that heavy alcohol consumption also contributes to cancer. The odds of correctly identifying heavy alcohol consumption as a risk factor for diabetes were 3.00 times (95% confidence interval (CI): 1.29,7.00) higher among smokers but nondrinkers than smokers and alcohol drinkers. Education level was significantly associated with participants' awareness of risk factor (p < 0.01). There is an urgent need for targeted educational campaigns and interventions to increase understanding of the impact of heavy alcohol consumption on cancer risk.

Association between PNPLA3 and TM6SF2 gene polymorphisms and non-alcoholic fatty liver disease patients in Kazakhstan

&lt;b&gt;Background:&lt;/b&gt; Non-alcoholic fatty liver disease (NAFLD) is a growing burden on a global scale and considered as the most common liver disease of the 21&lt;sup&gt;st&lt;/sup&gt; century, affecting both adults and children. Genome-wide association studies (GWAS) in the field of liver diseases have made a significant contribution to the understanding of genetic background for NAFLD development. Targeted genes like PNPLA3 and TM6SF2 showed some relationship with the steatosis and hepatocellular carcinoma within NAFLD patients. In this study, we tried to analyze the frequency of PNPLA3 and TM6SF2 gene polymorphisms and their relationship to changes in instrumental and laboratory markers, the composition of the gut microbiome, the development and progression of NAFLD stage in Kazakhstan.&lt;br /&gt; &lt;b&gt;Materials and methods:&lt;/b&gt; 39 individuals were involved in this study, including 18 men and 21 women: patients with a history of heavy alcohol consumption (&amp;gt;20 g/day) and other specific diseases such as hepatitis B and C virus infection, etc. were excluded. The diagnosis was established based on the results of clinical assessment and laboratory-instrumental results. The microbiome composition of the large intestine was studied by semiconductor sequencing of the bacterial genome using biochips. The degree of steatosis and liver fibrosis were evaluated by fibroscanning on fibroscan touch 502. Genotyping of PNPLA3 and TM6SF2 were carried out by PCR.&lt;br /&gt; &lt;b&gt;Results:&lt;/b&gt; According to PNPLA3 genotyping: 21 patients (53.85%) were C/G, 7 (17.95%) were C/C and 11 (28.20%) were G/G. Within analyzed variables, GGT showed statistically significant difference among nucleotide variability with p-value of 0.012. Other parameters within metabolic panel also showed statistically significant difference between groups, namely, total cholesterol (p=0.036) and LDL (p=0.006). Genotyping of TM6SF2 includes 24 patients (61.54%) with C/C, 15 (38.46%) with C/T and 0 with T/T. The relationship between TM6SF2 liver function test results showed no statistically significant differences between groups. All other parameters including gut microbiome analysis are not statistically significant.&lt;br /&gt; &lt;b&gt;Conclusions:&lt;/b&gt; In this study, C/G genotype possesses the highest risk and GGT along with LDL were the statistically significant parameter between them in PNPLA3 gene. TM6SF2 and gut microbiome analysis did not reveal any statistically significant differences. Additional studies with larger sample size are recommended to obtain for more detailed and sensitive results.

Investigating Diabetic Foot Pathophysiology and Amputation Prevention Strategies through Behavioral Modification

Diabetic foot complications stem from intricate interactions between macrovascular and microvascular changes, neuropathy, inflammation, immune responses, hyperglycemia, oxidative stress, and infection susceptibility. Macrovascular factors like atherosclerosis lead to tissue ischemia, while microvascular dysfunction worsens perfusion deficits. Neuropathy contributes significantly to such complications, causing sensory loss, motor problems, and autonomic dysfunction. This results in unnoticed injuries, muscle atrophy, deformities, and dry skin, elevating the risk of non-healing wounds and infections. Inflammation and immune responses amplify tissue damage and hinder healing. Chronic hyperglycemia generates advanced glycation end products, stiffening tissues, while oxidative stress exacerbates damage. Mitochondrial dysfunction further compromises cellular energy production, worsening tissue repair challenges. These multifaceted factors collectively contribute to diabetic foot complications. The impact of modifiable behavior factors such as smoking, heavy alcohol consumption, and exercise on the risk of lower extremity amputation (LEA) in diabetic patients is also not to be ignored. Substantial data had identified increased LEA risk from smoking and heavy alcohol intake and reduced risk from regular exercise. The cumulative impact of these behaviors underscores the significance of behavior modification in preventing LEA and enhancing the well-being of diabetic patients. Understanding these mechanisms is vital for developing effective preventive strategies, diagnostics, and treatments, addressing the impact of diabetic foot complications on individuals and healthcare systems.

Detrimental Effects of Alcohol on the Heart: Hypertension and Cardiomyopathy.

Epidemiological evidence suggests a J-shaped association between alcohol consumption and cardiovascular mortality, with higher cardiovascular event rates occurring among abstainers and heavy drinkers compared to moderate consumers. However, this hypothesis has been challenged by more recent studies. Furthermore, ethnicity, gender, type of alcoholic beverage, and pattern of alcohol intake, influence the relationship between alcohol and heart health. We undertook a review of the relavent literature utilizing PubMed. Heavy alcohol consumption causes resistant hypertension, cardiomyopathy, arrhythmias, hemorrhagic strokes, as well as hepatic cirrhosis and pancreatitis. Excessive drinking is the third most preventable cause of death worldwide behind hypertension and smoking. In this review, we discuss the effects of alcohol abuse on hypertension (a major cause of myocardial infarction and stroke) and alcoholic cardiomyopathy. Another article in this Special Issue "Alcohol and Heart Health" discusses the problem with alcohol and arrhythmias sudden cardiac death.

Cancer risk based on alcohol consumption levels: a comprehensive systematic review and meta-analysis.

Alcohol consumption is a well-established risk factor for cancer. Despite extensive research into the relationship between alcohol consumption and cancer risk, the effect of light alcohol consumption on cancer risk remains a topic of debate. To contribute to this discourse, we conducted a comprehensive systematic review and meta-analysis. Our systematic review aimed to investigate the associations between different levels of alcohol consumption and the risk of several cancer types. We focused on analyzing prospective associations using data from 139 cohort studies. Among them, 106 studies were included in the meta-analysis after a quantitative synthesis. Our analysis did not find a significant association between light alcohol consumption and all-cause cancer risk (relative risk, 1.02; 95% confidence interval, 0.99 to 1.04), but we observed a dose-response relationship. Light alcohol consumption was significantly associated with higher risks of esophageal, colorectal, and breast cancers. Light to moderate drinking was associated with elevated risks of esophageal, colorectal, laryngeal, and breast cancers. Heavy drinking was also found to contribute to the risk of stomach, liver, pancreas, and prostate cancers, thereby increasing the risk of almost all types of cancer. Additionally, females generally had lower cancer risks compared to males. Our findings highlight that cancer risks extend beyond heavy alcohol consumption to include light alcohol consumption as well. These findings suggest that there is no safe level of alcohol consumption associated with cancer risk. Our results underscore the importance of public health interventions addressing alcohol consumption to mitigate cancer risks.

OR24-06 The Role Of Oxidative Stress In The Association Of Smoking And Alcohol Consumption With Hepatic Steatosis

Abstract Disclosure: M. Shaheen: None. K. Schrode: None. D. Pan: None. S.M. Najjar: None. T.C. Friedman: None. Hepatic steatosis (HS) is the most prevalent chronic liver disease in the United States. While heavy alcohol consumption is known to be a risk factor for HS, the effect of moderate consumption is still debated. Similarly, while smoking is a known risk factor for many chronic conditions, its contribution to HS is still in question. A potential mechanism by which these factors could increase risk for HS is through oxidative stress. Oxidative stress is an imbalance between free radicals and antioxidants in the body that causes inflammation and cellular damage. Behaviors such as smoking and alcohol consumption are thought to increase oxidative stress. This study aims to determine the role of oxidative stress in the relationship between smoking and moderate alcohol consumption with HS among the adult population of the United States. We analyzed data for 3444 participants from the National Health and Nutrition Examination Survey 2017-2018. Hepatic steatosis was measured by FibroScan® using controlled attenuation parameter (CAP) values, and we used levels of γ-glutamyltransferase (GGT) as a biomarker for oxidative stress. We categorized alcohol consumption and smoking as none, former (for smoking), light, and moderate-heavy following the National Center for Health Statistics. We used bivariate chi square analysis and multivariable logistic regression adjusting for the demographics, laboratory, and co-morbidity and consider the design and weight. We developed two models with and without oxidative stress. In bivariate analyses, oxidative stress was significantly associated with heavier smoking and alcohol consumption, and with HS (all p&amp;lt;0.05). A multivariable model without oxidative stress showed that light drinking alone, light smoking alone, and combination of light smoking and light drinking were not associated with HS (p&amp;gt;0.05), but moderate-heavy smoking combined with light drinking or moderate-heavy drinking increased the odds of HS compared to abstinence from both smoking and drinking (Adjusted Odds Ratio [AOR]=3.9, 95% Confidence Interval [95% CI]= 1.5 - 10.2, p=0.006; AOR=6.2, 95% CI= 1.4 - 26.9, p= 0.014, respectively). When we included oxidative stress in the model, oxidative stress was independently associated with HS (AOR=1.1, 95% Ci=1.0-1.1, p=0.011) and the odds ratios for the interaction of smoking and alcohol did not change, indicating that oxidative stress was not a mediator of these relationships. Our findings indicated that smoking and alcohol consumption can interact to increase the odds of HS in the US population independent of oxidative stress. Further research is needed to identify the underlying mechanisms.Grants: R01MD012579, R24DA017298, U54MD007598, S21MD000103, UL1TR001881 Presentation: Saturday, June 17, 2023