Heavier Body Weight Research Articles

In ovo betaine and heat acclimation affect hatching results, growth performance and immunity of the broilers under cyclicheatstress.

This study investigated the effects of in ovo betaine and thermal manipulation during incubation on growth performance, and some immune parameters of broilers under cyclic heat stress (CHS). Eggs were divided into 5 groups and incubated at 1) 37.8°C and 60% relative humidity (Control incubation and not-injected, CI); 2) eggs were incubated at CI and in ovo betaine injected into yolk sac on d 11 (E11) (CI + In ovo); 3) eggs were exposed to 38.8°C for 8h between 10 and 18d of incubation (heat acclimation, HA); 4) eggs were incubated at HA and in ovo betaine applied (HA + In ovo); 5) positive control: eggs were incubated at CI and injected with saline. Hatched chicks were raised under standard management conditions until 21 d, between 21 and 42 d half of the chickens in each incubation treatment were kept either at optimum (OPT) or at CHS. In ovo and HA did not affect hatching performance. In ovo increased thymus and spleen weights of chicks. Serum IgG was higher in HA + In ovo chicks. From d 7 to 21, in ovo chicks were heavier body weights, consumed more feed, and better feed conversion than those from CI. The body weights of HA chickens were similar in OPT and CHS on d 28 and 35. CHS reduced the body weight of CI chickens which was compatible with their feed consumption. Moreover, feed intake of HA + In ovo chickens exposed to CHS was higher than those not injected indicating that HA + In ovo enhanced thermoregulation of chickens under CHS.

PSII-15 Comparative analysis of equine diets on growth metrics and economic feasibility

Abstract The objective of the study was to assess the impact of two commonly available low non-structural carbohydrate equine diets on growth parameters and economic factors in young growing horses. To do this, young stock type horses n = 11; 6 fillies, 5 colts; body weight (BW) = 380.3 ± 15 kg] were separated into two groups that were balanced by age, sex, and BW. All horses were backgrounded for 14 d on a hay-only diet prior to the beginning of the study. Additionally, each group was assigned to one of two dietary treatments fed at 0.75% BW/d (as fed) twice daily in 12 h intervals for 56 d. Dietary treatments consisted of a leading national brand feed (n = 5) and Thomas Moore Calm and Safe (n = 6). Horses were allowed ad libitum access to Coastal bermudagrass (Cynodon dactylon) hay and fresh water. For the 56-d period, horses were exercised at a light workload for 30 min/d and 4 d/wk. For all growth measurements, a baseline measurement was recorded per horse. Growth measurements were then recorded at 7 d intervals. Data from these growth measurements were analyzed using PROC MIXED in SAS v9.4 with the fixed effects of day and diet. Growth metrics increased on all horses throughout the length of the trial regardless of dietary treatment (P ≤ 0.01), which is expected from young growing horses. A Diet x Day trend towards significance (P = 0.075) was observed for BW, as the leading national brand feed dietary treatment group tended to have heavier BW than the Thomas Moore Calm and Safe during the duration of the study. Additionally, body fat percentage was greater (P = 0.04) in the national leading brand feed group compared with the Thomas Moore group. None of the remaining growth metrics had any statistical difference across diets. Furthermore, there was no difference in dietary intake between dietary groups (P > 0.5). These results suggest that the Thomas Moore Calm and Safe concentrate serves as a good alternative and economically friendly for young growing horses when compared with more traditional feeds currently on the market.

PSLBII-23 Effects of alternative production systems on growth and circulating blood profiles in Berkshire grower pigs

Abstract In this study, we compared the growth performance and circulating blood profiles of Berkshire grower pigs maintained in two different alternative swine production systems, the dry-lot production system, and the hoop structure production system. This study was conducted during a 75-d period spanning from May to July at the North Carolina Agricultural and Technical State University Swine Facility in Greensboro, N.C. Protein expression profiles associated with growth (FGF-19, PDGF-BB, IGF-1), feed intake (CCK), stress (cortisol) and the ability to handle oxidative stress (T-AOC) were analyzed in blood plasma obtained from 24 Berkshire nursery/grower pigs. Berkshire pigs (n = 12) were managed in a 12.19 m x 18.28 m hoop structure with the end walls removed for ventilation and a straw deep bedding system. Twelve additional Berkshire nursery/grower pigs were managed in a 0.08-ha dry-lot. These animals were provided with a canopy for shade and a wallowing area for evaporative cooling. All animals were provided with ad libitum ration and water. Individual body weights (BW) and blood samples were collected at 25-d intervals throughout the 75-d trial, culminating in three different time points. Plasma was isolated from the blood samples and used for ELISA and colorimetric assays. The BioTek 800 TS absorbance reader and Gen5 Microplate Reader and Imager software (Agilent Technologies, Inc., Santa Clara, CA) were used to obtain and calculate the optical densities of the plasma samples, respectively. The data were analyzed using the two-way ANOVA feature of JMP 17.2.0 software (SAS Institute, Inc., Cary, NC) with production system and growth period considered fixed variables, while either growth rate or protein concentration were considered as the response variable. There were four significant treatment effects observed (P < 0.05). Significantly greater concentrations of IGF-1, CCK, and T-AOC were detected in the plasma collected from Berkshire pigs managed in the dry-lot. Whereas those managed in the hoop structure production system had heavier BW. The results show that exposing pigs to higher temperatures can reduce feed intake and growth rates. However, this same stress elicits the release of hormones that promote growth, while enhancing the total antioxidant capacity in these animals. The results support the adoption of the hoop structure production system over the dry-lot production system when managing swine in regions that experience higher temperatures.

PSII-2 Does environmental feeder enrichment for weaned pigs drive feeder usage and improve final body weight?

Abstract Transition from the sow to nursery pens is a stressful time for pigs and commonly leads to post-weaning anorexia and growth check. This can have negative impacts on pig health, welfare, and lifetime performance. The objective of this study was to test the relationship between enrichment use and feeder use immediately post-weaning on final nursery pig body weight (BW). At arrival, 29 mixed-sex pigs (Camborough 1050 X 337, PIC), 19 to 24d of age, were randomly sorted into 3 pens (~0.29 m2/pig) at the Iowa State University Swine Nutrition Farm. Each pen was equipped with a 4-hole, dry self-feeder measuring 0.6 m long. Each pen had two enrichment devices, consisting of one cotton rope and two biscuits, suspended from the feeder at pig height. Biscuits were replaced daily. Total number of interactions with the enrichment and feeder were collected at the individual pig level. All pigs were continually observed for two 1 h periods (h 1 and the h 6 after biscuit replacement) each day over wk 1 (d 0 – d 6). A pig was considered interacting with enrichment if its snout contacted the rope or enrichment biscuit. A pig was considered interacting with the feeder if 1) its head was over the feeder tray, and 2) its nose down in the feeder. Both enrichment and feeder interactions were considered terminated if the pig ceased active interaction for 3 s or more. Behaviors were summed together by time period and day for each pig. Individual BW were taken at the end of the 42-d nursery study. After the nursery trial was completed, the 29 pigs were allocated to a light BW treatment (n = 14) and a heavy BW treatment (n = 15) using the final average BW (24.22 kg) of the. Pigs with a final BW lighter than the group average were assigned to the light treatment, while pigs with a heavier BW than average were assigned to the heavy treatment. T-tests were conducted for enrichment and feeder interactions by final BW group and the pig was the experimental unit. A P ≤ 0.05 was considered significant. There were no observed differences between final pig body weight treatments and the enrichment and feeder interactions over the first week of nursery (P ≤ 0.87). Numerically, there were more overall enrichment interactions than feeder interactions for both BW treatments over wk 1 (Table 1). In conclusion, this enrichment device was of biological interest to the pigs, but other factors impact feeder usage in the first week and need to be considered in future work.

Effects of Mink-Origin Enterococcus faecium on Growth Performance, Antioxidant Capacity, Immunity, and Intestinal Microbiota of Growing Male Minks.

The purpose of this experiment was to explore the effects of dietary Enterococcus faecium (EF) on the growth performance, antioxidant capacity, immunity, and intestinal microbiota of growing male minks. A total of 60 male Regal White minks at 12 weeks of age were randomly assigned to two groups, each with 15 replicates of two minks per replicate. The minks in two groups were fed the basal diets and the basal diets with viable Enterococcus faecium (more than 107 cfu/kg of diet), respectively. Compared with the minks in control, Enterococcus faecium minks had heavier body weight (BW) at week 4 and week 8 of the study (p < 0.05), greater average daily gain (ADG), and a lower feed/gain ratio (F/G) of male minks during the initial 4 weeks and the entire 8-week study period (p < 0.05). Furthermore, Enterococcus faecium increased the apparent digestibility of crude protein (CP) and dry matter (DM) compared to the control (p < 0.05). Moreover, Enterococcus faecium enhanced the serum superoxide dismutase (SOD) activity and decreased the malondialdehyde (MDA) contents (p < 0.05). The results also confirmed that Enterococcus faecium increased the levels of serum immunoglobulin A (IgA), immunoglobulin G (IgG), and the concentrations of secretory immunoglobulin A (SIgA) in the jejunal mucosa while decreasing the interleukin-8 (IL-8) and interleukin-1β (IL-1β) levels in the jejunal mucosa (p < 0.05). Intestinal microbiota analysis revealed that Enterococcus faecium increased the species numbers at the OUT level. Compared with the control, Enterococcus faecium had significant effects on the relative abundance of Paraclostridium, Brevinema, and Comamonas (p < 0.05). The results showed that Enterococcus faecium could improve the growth performance, increase the antioxidant capacity, improve the immunity of growing male minks, and also modulate the gut microbiota.

Estimation of genetic correlations and genomic prediction accuracy for reproductive and carcass traits in Hanwoo cows.

This study estimated the heritabilities (h2) and genetic and phenotypic correlations between reproductive traits, including calving interval (CI), age at first calving (AFC), gestation length (GL), number of artificial inseminations per conception (NAIPC), and carcass traits, including carcass weight (CWT), eye muscle area (EMA), backfat thickness (BF), and marbling score (MS) in Korean Hanwoo cows. In addition, the accuracy of genomic predictions of breeding values was evaluated by applying the genomic best linear unbiased prediction (GBLUP) and the weighted GBLUP (WGBLUP) method. The phenotypic data for reproductive and carcass traits were collected from 1,544 Hanwoo cows, and all animals were genotyped using Illumina Bovine 50K single nucleotide polymorphism (SNP) chip. The genetic parameters were estimated using a multi-trait animal model using the MTG2 program. The estimated h2 for CI, AFC, GL, NAIPC, CWT, EMA, BF, and MS were 0.10, 0.13, 0.17, 0.11, 0.37, 0.35, 0.27, and 0.45, respectively, according to the GBLUP model. The GBLUP accuracy estimates ranged from 0.51 to 0.74, while the WGBLUP accuracy estimates for the traits under study ranged from 0.51 to 0.79. Strong and favorable genetic correlations were observed between GL and NAIPC (0.61), CWT and EMA (0.60), NAIPC and CWT (0.49), AFC and CWT (0.48), CI and GL (0.36), BF and MS (0.35), NAIPC and EMA (0.35), CI and BF (0.30), EMA and MS (0.28), CI and AFC (0.26), AFC and EMA (0.24), and AFC and BF (0.21). The present study identified low to moderate positive genetic correlations between reproductive and CWT traits, suggesting that a heavier body weight may lead to a longer CI, AFC, GL, and NAIPC. The moderately positive genetic correlation between CWT and AFC, and NAIPC, with a phenotypic correlation of nearly zero, suggesting that the genotype-environment interactions are more likely to be responsible for the phenotypic manifestation of these traits. As a result, the inclusion of these traits by breeders as selection criteria may present a good opportunity for developing a selection index to increase the response to the selection and identification of candidate animals, which can result in significantly increased profitability of production systems.

Back pain in adolescent idiopathic scoliosis: frequency and risk factors

PurposeAlthough back pain is commonly reported in patients with adolescent idiopathic scoliosis (AIS), factors that influence the presence and severity of back pain in AIS, including curve-specific variables, have not been well studied. This study aims to describe the prevalence and severity of back pain in AIS patients and determine the extent to which patient characteristics, including curve-specific factors, are associated with a higher risk of back pain in AIS.MethodsThe study was a retrospective medical records review of adolescents (aged 10–17 years) diagnosed with AIS between 01/01/2018 and 12/31/2021 at an academic tertiary children’s hospital. Patients with previous spine surgery were excluded. Variables collected included demographics (age, sex, race, insurance), Lenke classification, major coronal curve, back pain-related information, Risser stage, vitamin D levels, post-diagnosis brace utilization, physical therapy or chiropractic treatment, and surgery.ResultsA total of 891 AIS patients were included in the analysis. The sample was predominantly female (73.3%) and insured by Medicaid (57.8) with a mean age of 12.8 years. The mean major coronal curve was 26.3 degrees. Most patients had Lenke type 1 (47%) and type 5 (41%) curves. Nearly half of patients reported back pain (48.5%) with average pain severity in the low-to-moderate range (4.7) on FACES pain scale (0–10). Among those who reported back pain, 63.2% specified a location with the majority reporting pain in the lumbar region (56%) and, less commonly, in the thoracic (39%) and scapular (8%) regions. Lumbar pain was associated with significantly higher pain intensity (p = 0.033). Additionally, the location of pain reported was associated with location of major coronal curve (p < 0.0001). No association was observed between pain presence and vitamin D deficiency (p = 0.571, n = 175), major coronal curve magnitude (p = 0.999), Lenke curve type (p = 0.577), and sex (p = 0.069). Older patients, those insured by Medicaid, and those with higher Risser scores were more likely to report pain scores (p = 0.001 for all).ConclusionNearly half (48%) of newly diagnosed AIS patients experience back pain which is higher than the prevalence of 33% seen in the general adolescent population. Pain was more prevalent among patients over the age of 13, with heavier body weight, and those insured by Medicaid. Pain was most commonly reported in the lumbar region, especially among patients with lumbar curves. This information can be helpful in counseling AIS patients, though further investigations are needed, especially to determine the underlying causes of back pain in AIS and to elucidate the discrepancy in pain between patients with Medicaid and commercial insurance.Level of evidence.Prognostic Study Level II.

Bigger isn't always better: Challenging assumptions about the associations between diapause, body weight, and overwintering survival.

During the winter, animals face limited food availability. Many animals enter dormancy to reduce their winter energy expenditure. Most insects spend the winter in diapause, a state of programmed dormancy. It is often assumed that diapausing insects need nutrient stores to fuel their many months of basal metabolism and must grow heavier than their non-diapause-programmed counterparts. However, the extent to which food limitation affects body weight during overwintering preparation as well as the likelihood and duration of diapause remainsunclear. We limited the duration of the feeding period and thus the total quantity of food available to diapause-destined larvae of the pupal-diapausing flesh fly, Sarcophaga crassipalpis, to test how food limitation affects body weight in the context of diapause programming. We also tested the extent to which food deprivation and body weight affect the likelihood and duration of diapause. We hypothesized that diapause-destined larvae grow more quickly and pupariate at a heavier body weight than non-diapause larvae. We also hypothesized that body weight is more dramatically reduced by food limitations when a larva is programmed for diapause. Finally, we hypothesized that larvae with lighter body weight (i.e., food limited) are less likely to enter pupal diapause and also stay in diapause for a shorter duration than heavier, well-fed, individuals. Contrary to our hypotheses that diapausing insects are heavier than their non-diapausing counterparts, we found diapausing pupae weighed less than non-diapausing pupae, especially when larvae received limited food. We found light pupae did not abort their diapause program. In both diapausing and non-diapausing pupae, body weight was positively correlated with simulated winter survival. However, above a weight threshold, body weight no longer affected simulated winter survival in diapausing pupae. Contrary to our predictions and the general consensus in much of the diapause literature, we also found that lighter pupae stayed in diapause longer than heavier pupae. Overall, our results challenge the precept that body weight and diapause are positively associated. The relationship between body weight and diapause is complex and may be affected by the availability of food before and after winter, the availability of high-quality overwintering sites, and the life history of a particular insect.

Effect of energy level of pullet diet and age on laying performance and expression of hypothalamus-pituitary-gonadal related genes in laying hens

Dietary energy density influences feed intake (FI) and development of layer-type pullets. A total of 384 nine-wk-old Hy-Line Brown pullets were randomly assigned to one of 3 dietary treatments: fed a diet with 2,600, 2,750, and 2,900 Kcal metabolizable energy/kg (ME/kg) from 10 to 21 wk of age. The results showed that the 2,900 and 2,600 ME groups had lower feed and ME intake (P < 0.01) from 10 to 21 wk of age. The 2,600 ME pullets had heavier body weight (BW) and longer shank length (P < 0.05) at 21 wk of age than the 2,750 ME group. The eggshell percentage was increased by the 2,600 and 2,900 kcal/kg treatments (P = 0.002). Serum concentration of 17-β-estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) decreased at 70 wk of age (P < 0.05). Pullet diet and its interaction with age had a significant influence (P < 0.001) on the expression of gonadotropin-releasing hormone 1 (GnRH-1) and gonadotropin-inhibitory hormone (GnIH) in the hypothalamus and of gonadotropin releasing hormone 1 receptor (GnRH-1R) and gonadotropin-inhibitory hormone receptor (GnIHR) in the pituitary. In the hypothalamus, GnRH-1 expression increased from 9 to 40 wk of age and then decreased; however, GnIH expression was highest at 70 wk of age. Follicle-stimulating hormone receptor (FSHR) expression increased (P < 0.001) at wk 40 and decreased at wk 70 compared to wk 21 at various follicular stages. In conclusion, the energy level of pullet diet had no unfavorable influence on feed intake, laying rate, egg mass, and FCR, whereas change egg weight and mortality during the laying period from 21 to 70 wk of age. during the laying period. These results suggest that pullet dietary energy can activate the expression of genes related to reproduction in the hypothalamus, whereas it plays a minor role in the regulation of genes in the pituitary and ovary. Age-induced gene expression in the hypothalamus-pituitary-gonadal (HPG) axis is associated with laying performance in hens.

PSIV-A-3 Effect of fiber and stimbiotic on growth performance and the gut microbiome in pigs challenged with F18 enterotoxigenic Escherichia coli (ETEC)

Abstract Dietary fiber has been shown to promote a healthy gut microbiota, while stimbiotics enhance fermentable substances and boost the expansion of the commensal microbiome, improving host health by alleviating deleterious effects of proinflammatory responses. The intended purpose of this study was to gain an understanding of the effectiveness of dietary fiber sources and stimbiotic on the sustainability of F18 enterotoxigenic Escherichia coli (ETEC) challenge. A total of 216 piglets were blocked (n = 8) by weaning body weight (BW) and randomly assigned to 1 of 4 dietary treatments, including corn-SBM based control diet (CC), high fiber diet (HF: 5.0, 3.8. 2.5% sugar beet pulp and 4.0, 3.0 and 2.0% soy hulls for phases 1, 2, and 3, respectively), CC plus 0.01% stimbiotic (CS: Signis, ABVISTA Marlborough, UK), and HF plus 0.01% stimbiotic (FS). Pigs were fed a four-phase feeding regimen: phase 1 (d 0-9), phase 2 (d 9-16), phase 3 (d 16-22), phase 4 (d 22-41). Dietary fiber and stimbiotic were provided from weaning (d 0) to d 22, and then a common diet was fed from d 22 to the end of the trial. On d 5 postweaning, all pigs were orally inoculated with 10 mL of F18 ETEC (108 CFU/mL). One median BW pig from each pen was selected at weaning for fecal sampling. Before the challenge, the same pigs were sampled on d 5 and again on d 8 and d12. Samples were used to quantify the E. Coli using 3M Petrifilm (3M Food Safety, St. Paul, MN) and virulence genes of the isolate were qualified using multiplex PCR. Colony-forming-count was log-transformed. All data normality was verified using Univariate procedure and analyzed using Mixed procedure of SAS (Cary, NC). Pigs fed both CS and HF increased average daily gain (ADG) in phase 2 and phase 1-3 compared with pigs fed CC diets, while adding stimbiotic had no additional benefit to HF (Fiber×Stimbotic P = 0.03 and 0.06, respectively). This response led to heavier BW in pigs fed CS at the end of phase 3 compared with pigs fed CC with HF and FS as intermediate (Fiber×Stimbotic P = 0.06, respectively). Overall, average daily feed intake (ADFI) was greater in pigs fed CS than pigs fed FS while no difference was found between CC and CF (Fiber×Stimbotic P = 0.02). Dietary fiber did not impact gain to feed ratio (G:F); however, G:F was improved in stimbiotic fed pigs in phase 2 (Stimbiotic P = 0.01). Total coliform count was reduced from d 2 to d 7 (day; P &amp;lt; 0.01), but neither fiber nor stimbiotic altered the coliform count. Results of this study demonstrated that both high fiber and stimbiotic help restore ADG when pigs were challenged with ETEC. Additionally, stimbiotic also benefited feed efficiency and resulted in the heaviest BW.

Influence of BOVAMINE DEFEND Plus on growth performance, carcass characteristics, estimated dry matter digestibility, rumen fermentation characteristics, and immune function in finishing beef steers.

One hundred and eighty crossbred beef steers (406.0 ± 2.2kg) were used to determine the impact of a novel direct-fed microbial (DFM) on growth performance, carcass characteristics, rumen fermentation characteristics, and immune response in finishing beef cattle. Steers were blocked by body weight (BW) and randomly assigned, within block, to 1 of 2 treatments (3 replicates/treatment: 30 steers/replicate). Treatments included: (1) no DFM (control) and (2) DFM supplementation at 50 mg ∙ animal-1 ∙ d-1 (BOVAMINE DEFEND Plus). All steers were fed a high-concentrate finishing diet and individual feed intake was recorded daily via the GrowSafe system. BWs were collected every 28 d. On day 55, 10 steers per pen were injected with ovalbumin (OVA). Jugular blood samples were collected from each steer on days 0, 7, 14, and 21 post injection. On day 112, the same steers were injected again with OVA and intramuscularly with a pig red blood cell solution. Jugular blood samples were collected from each steer on days 0, 7, 14, and 21 post injection. On day 124, rumen fluid was collected from 3 steers per treatment and used to estimate in vitro rumen fermentation characteristics. Equal numbers of steers per treatment were transported to a commercial abattoir on days 145, 167, and 185 of the experiment, harvested, and carcass data were collected. Initial BW was similar across treatments. On days 28 and 55, steers receiving DFM had heavier BW (P < 0.01) compared to controls. The average daily gain was greater in DFM-supplemented steers from days 0 to 28 (P < 0.01) and days 0 to 55 (P < 0.01) of the experiment compared to controls. Overall dry matter intake (DMI) was greater (P < 0.04) and overall feed efficiency was similar in DFM-supplemented steers compared to controls. Dressing percentage (P < 0.02) was greater in steers receiving DFM compared to controls. Antibody titers to injected antigens were similar across treatments. However, red blood cell superoxide dismutase activity was greater (P < 0.05) in DFM-supplemented steers compared to controls. In vitro molar proportions of isobutyric and butyric acid were greater (P < 0.01) and dry matter (DM) digestibility tended (P < 0.07) to be greater in rumen fluid obtained from steers supplemented with DFM. These data suggest that BOVAMINE DEFEND Plus supplementation improves growth performance during the initial period of the finishing phase, increases overall DMI and dressing percentage, and may impact antioxidant status in beef cattle.

Vitamin E: An assistant for black soldier fly to reduce cadmium accumulation and toxicity

Cadmium (Cd) is a toxic heavy metal associated with osteoporosis, liver, and kidney disease. The black soldier fly (BSF) Hermetia illucens may be exposed to Cd during the transformation of livestock manure. The BSF has a high tolerance to Cd. In the previous work of the laboratory, we found that vitamin E (VE) may play a role in the tolerance of BSF to Cd exposure. The main findings are as follows: The BSF larvae pretreated with exogenous VE had heavier body weight, lower content and toxicity of Cd under similar Cd exposure. Even in high Cd exposure at the concentrations of 300 and 700 mg/kg, the BSF larvae pretreated with exogenous VE at a concentration of 100 mg/kg still reduced the Cd toxicity to 85.33 % and 84.43 %, respectively. The best-fitting models showed that metallothionein (MT) content, oxidative damage (8-hydroxydeoxyguanosine content, malondialdehyde content), antioxidant power (total antioxidant power, peroxidase activity) had a great influence on content and toxicity of Cd bioaccumulated in the larvae. The degree of oxidative damage was reduced in the larvae with exogenous VE pretreatments. This variation can be explained by their changed MT content and increased antioxidant power because of exogenous VE. These results reveal the roles of VE in insects defense against Cd exposure and provide a new option for the prevention and therapy of damage caused by Cd exposure.

The Effect of Comb Cell Size on the Development of Apis mellifera Drones.

The growth and development of honeybees are influenced by many factors, one of which is the cell size of the brood comb. Larger worker bees can be obtained by being raised in bigger cells. However, whether cell size has the same effect on drone development is still unknown. Here, using 3D-printed foundations, we observed the development of drones kept in comb cells of different sizes from the late larval stage through eclosion. The results showed that drones in larger cell-size combs had heavier body weights, longer body lengths, and larger head widths, thorax widths, and abdomen widths compared to those in smaller cell-size combs. Furthermore, regardless of developmental stages, the drones' body weights increased linearly with the comb's cell size. However, the other morphological changes of drones in different developmental stages were out of proportion to the cell-size changes, resulting in smaller cells with a higher fill factor (thorax width/cell width). Our findings confirm that comb cell size affects the development of honeybees; drones become bigger when raised in large cells.

Blood flow rate to the femur of extinct kangaroos implies a higher locomotor intensity compared to living hopping macropods

The stocky skeletons and post-cranial anatomy of many extinct kangaroos indicate that they might have engaged in varied locomotor behaviors, rather than bipedal hopping, as their primary mode of locomotion. This study investigates support for this idea by estimating femoral bone perfusion, which is a correlate of locomotor intensity, in extinct kangaroos compared to living hopping species. Femur blood flow rates can be estimated from the sizes of nutrient foramina on the femur shaft of living and extinct species, without preservation of soft tissue. Estimated femur blood flow rates among the extinct Macropus, Protemnodon and Sthenurinae (Sthenurus, Simosthenurus and Procoptodon) are not significantly different from one another but are significantly greater than in living hopping macropods after accounting for the effect of body mass, consistent with their purportedly different locomotor style. The giant sthenurines have more robust femora than extrapolated from data of living hopping macropods, possibly due to the larger sthenurines requiring relatively stronger leg bones to support their heavier body weights, especially if loaded onto a single limb during bipedal striding.

Population pharmacokinetics of rotigotine extended-release microspheres for intramuscular injection in patients with early-stage Parkinson's disease.

Rotigotine extended-release microspheres is a weekly intramuscular injection formulation to treat Parkinson's disease. This study aimed to develop a population pharmacokinetics (PK) model for rotigotine extended-release microspheres to investigate its PK ethnic differences. Data for the study were obtained from three studies in China, Japan and the US. The population PK model was developed using the Phoenix NLME 8.3.5 software. Two parallel absorption models were created to include both zero- and first-order absorptions. The elimination phase was evaluated for one- and two-compartment linear models. Moreover, covariates including sex, body weight, body mass index, albumin, creatinine clearance and race were input into the model using a stepwise covariate method. We constructed a one-compartment linear model with the first parallel absorption model identified as the best-fitting model. Simulation results in patients with lighter body weight (45 kg) exhibited a 27% increase in Cmax,ss and a 31% increase in AUCtau,ss compared to those with median body weight (65 kg). Patients with heavier body weight (103 kg) showed a 27% decrease in Cmax,ss and a 29% decrease in AUCtau,ss compared to the median body weight group. Asian patients displayed only a 21% increase in Cmax,ss and a 6% increase in AUCtau,ss compared to non-Asian. While we could not fully conclude that race does not affect rotigotine exposure, dosage adjustments based on race were not deemed necessary. Exposure differences were mainly attributed to body weight, while dose adjustments were not needed for patients of different racial identities.

PSIV-12 Dietary Supplementation of a Botanical Blend Improved Growth Performance and Reduced Diarrhea Incidence in Commercially Housed Weaned Pigs

Abstract The study evaluated the effects of dietary botanical supplementation on the growth performance and diarrhea incidence of commercially housed weaned piglets. The study design was a randomized complete block with two dietary treatments [control (CON) and botanical blend (BB)] and 36 replicate pens per treatment (16 pigs/pen, 576 pigs/treatment). All piglets were fed a three-phase feeding program: P1, days 1-10; P2, days 11-21; P3, days 21-42. Diets were corn-soy and contained 150 ppm total zinc; P1 and P2 diets also contained 250 ppm of chlortetracycline. The botanical supplement was included in the P1 and P2 BB diets at a rate of 250 ppm. Body weight (BW) and feed disappearance were recorded weekly to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR). Body condition scoring and diarrhea frequency and severity were measured weekly for the first 4 weeks. Data were analyzed using PROC GLMMIX of SAS with appropriate models for the different data distributions. Pen was the experimental unit. The overall herd mortality and culling rate were 0.17% and 3.04%, respectively. Further, more than 95% of the pigs were in good body condition and did not differ between treatments. Pigs fed BB had a greater survival rate at the end of the study (97.9 vs 95.7%; P = 0.01) and diarrhea incidence was reduced by 5% for pigs fed BB compared with CON. In early nursery (days 1-21), pigs fed BB had a lower FCR (1.21 vs. 1.23 kg/kg; P = 0.049) and greater ADG (0.276 vs. 0.267 kg/d; P = 0.016), resultant in heavier BW at day 21 (12.1 vs. 11.9 kg; P = 0.046) than those fed CON. Considering the entire nursery (days 1-42), pigs fed BB tended to have greater ADG (0.430 vs 0.421 kg/d; P = 0.06) and ADFI (0.580 vs. 0.568 kg/d; P = 0.054) overall compared with CON. In conclusion, dietary supplementation of the botanical blend reduced diarrhea incidence and significantly improved early (1-21 days post-weaning) growth performance and feed efficiency in commercially housed weaned piglets. These early growth benefits persisted to the end of the nursery period, as a tendency for increased growth rate and feed intake.

199 Effects of an Additional Injection of Iron Administered to Piglets on Hemoglobin Concentration, Growth Performance, and Carcass Characteristics Through Market Weight

Abstract An experiment was conducted to evaluate the effects of an additional injection of iron dextran (Uniferon, Pharmacosmos Inc.) administered at d 6 to 8 of age. Crossbred pigs [n = 144; equal barrows and gilts; initial body weight (BW) = 2.86 ± 0.01 kg] from 21 litters were assigned to either the control (CON) or an added-injection treatment (+Fe). Pigs were paired by sex and BW within a litter and randomly assigned to the iron treatment within each pair. All pigs had received a 200 mg Fe intramuscular (IM) injection &amp;lt; 24 h after birth. All pigs were weaned at 22 to 24 days, housed 6 pigs/pen for a total of 12 pens/treatment, and received a common corn-soybean meal diet formulated to meet or exceed the NRC (2012) requirement estimates relative to BW until slaughter. Body weight and feed disappearance were recorded at least biweekly to determine average daily gain (ADG) and feed intake (ADFI). Hemoglobin (Hb) concentrations were measured at birth, initiation of experiment (d 6 to 8), weaning, and at the end of the nursery and finisher periods. At the time of slaughter, 1 pig/pen (n = 12 pigs/treatment) was selected and slaughtered for carcass characteristic measures. Data were subjected to ANOVA by the GLM procedure of SAS; the individual pig served as the experimental unit for BW, Hb, ADG, and carcass characteristics whereas pen served as the experimental unit for ADFI, and gain/feed ratio data. The statistical model included sex, treatment, sex × treatment, and allotment pair for Hb and ADG. There were expected sex effects for ADG but no sex × treatment interactions for any response measures. The +Fe pigs had a greater Hb at weaning (13.1 vs. 10.7 g/dL, P &amp;lt; 0.0001) and end of the nursery (12.1 vs. 11.7 g/dL, P = 0.01) compared with CON pigs (Table 1). During the finisher period, +Fe pigs had a greater ADG (P = 0.05) compared with CON pigs. Overall, pigs receiving the added iron injection had an ~ 4% increase in ADG (P = 0.04) from weaning to slaughter. The cumulative improvement in ADG from weaning to slaughter observed for +Fe group resulted in +Fe pigs having a heavier BW at the end of the experiment (115.77 vs. 112.79 kg; P = 0.04). Following slaughter, +Fe pigs had ~ 2.5% heavier cold carcass weight (82.99 vs. 80.93 kg; P = 0.34) and trimmed loin (10.67 vs. 9.95 kg; P = 0.04) compared with the CON pigs. In conclusion, administering an additional iron injection resulted in greater Hb at weaning and the end of the nursery as well as improved growth performance from weaning to slaughter weight and increased carcass measures at slaughter.

PSII-5 Impacts of Birth Body Weight Classification on Performance, Feed Efficiency, and Structural Characteristics in Beef Steers

Abstract The objective was to evaluate the relationship of birth body weight (BBW) classification on performance, feed efficiency, and structural characteristics in fall-born beef steers. Steers were classified based on BBW as either light (LtBBW; &amp;lt; -0.5 SD from mean), average (AvgBBW; ± 0.5 SD from mean), or heavy (HvyBBW; &amp;gt; +0.5 SD from mean). Steers (n = 167), either Angus or Hereford sired by commercial dams, were utilized in the analysis. Steers were early weaned at 81 ± 7 days of age. Following weaning, steers were managed in drylot pens until shipping on day 1 (140 ± 6 days of age). Upon feedlot arrival, steers were randomly assigned to pens and fed a growing diet for 87 days until transitioning to a finishing diet (DM basis; 45% high moisture corn, 25% corn silage, 20% modified wet distillers grains with solubles, 10% corn-based supplement). On day 98, steers began a 56-day evaluation utilizing GrowSafe system to determine dry matter intake (DMI). Steers were weighed monthly except during the intake evaluation period, where weights were collected biweekly. Foot, leg, and claw scores were collected for structural characteristics on days 58 and 237. Hip height was collected on day 237. As designed, birth body weight differed (P &amp;lt; 0.01) between classifications (30, 34, and 39 kg for LtBBW, AvgBBW, and HvyBBW, respectively). The BBW classification influenced (P &amp;lt; 0.01) body weight on days 1, 29, 59, 77, 98, 112, 126, 140, 154, 182, and 237; HvyBBW were heavier than LtBBW and AvgBBW, which were not different from each other. During the intake evaluation period, DMI was greater (P = 0.01) for HvyBBW compared with LtBBW and AveBBW, which did not differ from each other. Similarly, ADG differed (P = 0.03) by classification; HvyBBW was greater than LtBBW, with AvgBBW being intermediate but not different from either. Consequently, gain to feed did not differ (P = 0.44) by BBW classification. Following the intake evaluation, ADG tended (P = 0.09) to differ from day 154 to 237. Overall ADG from day 1 to day 237 differed (P = 0.01) by BBW classification; LtBBW were least and HvyBBW were greatest, with AvgBBW being intermediate but not different than either. Foot, leg, and claw scores were not different (P ≥ 0.15) by BBW classification on days 58 and 237. Hip height differed (P = 0.05) by BBW classification on day 237; HvyBBW were taller than LtBBW and AvgBBW, which did not differ from each other. In summary, heavy birth body weight steers had greater growth performance and DMI than light steers but did not differ in feed efficiency or structural characteristics.

Distribution and Prevalence of Elbow Pain (EP) in Elite Swimmers in Tianjin, China-A Regional Epidemiological Study.

Elbow pain (EP) in overhead sports is a common phenomenon. Swimming is classified as an overhead sport, but a lack of attention regarding EP in swimming has created a gap in the knowledge around elite freestyle swimming in Tianjin, China. The purpose of this study was to identify the distribution and prevalence of EP among elite freestyle swimmers in Tianjin, China. The methodology involved a total of 311 qualified participants who volunteered to undertake all measurements. The main findings showed that 183 of the elite freestyle swimmers in this study had EP (accounting for 58.84% of the total 311 participants), with 147 in slight pain and 36 in critical pain. The characteristics of a heavier body weight, taller height, longer left/right forearm length, and longer weekly average training hours serve as contributing factors to the occurrence of EP issues. Gender, age, weekly average training hours, and left/right forearm length are the primary factors correlating with and influencing the assessment scores. In conclusion, swimmers with longer weekly training hours, older ages, heavier bodyweight, and longer forearm lengths should receive greater attention in relation to EP. Regular assessments at a high frequency serve as a means to identify the risk of EP.

The effects of body weight and age on performance, egg quality, blood parameters, and economic production of laying hens

This study was conducted to investigate the effects of body weight on performance, egg quality, blood parameters, and economics of production of Lohmann white laying hens. A total of 288 Lohmann white layers, 44 wks of age, were allocated randomly to three groups, each formed 24 replicate cages as subgroups, comprising of four hens. The study was conducted over a period of 36 wks. Feed and water were offered ad libitum in the experiment. Performance parameters were significantly affected by body weight except for cracked egg. Considering the egg production, differences among the groups were significant (P&lt;0.001). Egg production in the light group was higher than that of the medium and the heavy group. However, egg weight was determined to be lower in the light group (66.58 g) than in medium (67.54 g) and heavy hens (68.84 g). Hens in light body weight had lower feed intake and feed conversion ratio (FCR) than hens in heavy body weight. There were no alterations in egg quality parameters in response to increased body weight except for SS and yolk color. SS decreased linearly (P&lt;0.001) and YC increased with BW. Other egg quality parameters did not change as BW increased. There was no change in the metabolic profile in response to increased BW except for glucose. Heavy hens had greater serum glucose concentrations than light and medium hens. This study emphasized that body weight affected the laying performance, some egg quality parameters, but had no significant effect on metabolic profile except for glucose. As a result, it was found that there was a positive relationship between the egg weight and the body weight of the hens. In this case, it is possible to produce more eggs with less feed by increasing the number of light and medium-weight chickens in the herd for profitable livestock.